##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934627.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	898962
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.135746561033727	31.0	31.0	33.0	28.0	34.0
2	31.178728355592337	31.0	31.0	34.0	28.0	34.0
3	30.975122418967654	31.0	31.0	34.0	28.0	34.0
4	34.937507925807765	35.0	35.0	37.0	32.0	37.0
5	35.15101973164605	35.0	35.0	37.0	33.0	37.0
6	35.10651618199657	36.0	35.0	37.0	32.0	37.0
7	30.81178848494152	35.0	35.0	37.0	0.0	37.0
8	32.89067168578872	35.0	35.0	37.0	17.0	37.0
9	36.30487718057048	39.0	35.0	39.0	32.0	39.0
10	36.02222785835219	37.0	35.0	39.0	32.0	39.0
11	36.23728700434501	37.0	35.0	39.0	32.0	39.0
12	36.294046911882816	38.0	35.0	39.0	32.0	39.0
13	36.27690046965278	38.0	35.0	39.0	32.0	39.0
14	37.34530825552137	39.0	36.0	40.0	32.0	41.0
15	37.482973696329765	39.0	36.0	41.0	32.0	41.0
16	37.393251327642325	39.0	36.0	40.0	32.0	41.0
17	37.279980688838904	39.0	36.0	40.0	32.0	41.0
18	37.17969391364707	39.0	36.0	40.0	32.0	41.0
19	36.96996758483673	39.0	35.0	40.0	31.0	41.0
20	36.95139060382975	39.0	35.0	40.0	31.0	41.0
21	36.98119275342005	39.0	35.0	40.0	31.0	41.0
22	37.05457627797393	39.0	35.0	40.0	32.0	41.0
23	37.09280481266171	39.0	35.0	40.0	32.0	41.0
24	36.99512771396344	39.0	35.0	40.0	31.0	41.0
25	36.69406382027272	38.0	35.0	40.0	31.0	41.0
26	36.61710617356462	38.0	35.0	40.0	31.0	41.0
27	36.66254858381111	39.0	35.0	40.0	31.0	41.0
28	36.6568475641907	39.0	35.0	40.0	31.0	41.0
29	36.60048033176041	39.0	35.0	40.0	31.0	41.0
30	36.35135411730418	38.0	35.0	40.0	30.0	41.0
31	36.333020750599026	38.0	35.0	40.0	30.0	41.0
32	36.146793746565486	38.0	35.0	40.0	30.0	41.0
33	36.00279322151548	38.0	35.0	40.0	30.0	41.0
34	35.86007194964859	38.0	35.0	40.0	29.0	41.0
35	35.73317782064203	38.0	35.0	40.0	28.0	41.0
36	35.71928179389118	38.0	35.0	40.0	29.0	41.0
37	35.62593747010441	38.0	34.0	40.0	28.0	41.0
38	35.47414684936627	38.0	34.0	40.0	27.0	41.0
39	35.353557769961355	38.0	34.0	40.0	27.0	41.0
40	35.26130692954764	38.0	34.0	40.0	26.0	41.0
41	35.06340090014928	38.0	34.0	40.0	26.0	41.0
42	35.099529234828616	38.0	34.0	40.0	26.0	41.0
43	35.02586316218038	38.0	34.0	40.0	26.0	41.0
44	34.83508869117938	37.0	34.0	40.0	25.0	41.0
45	34.68776210785328	37.0	33.0	40.0	24.0	41.0
46	34.55216015804895	37.0	33.0	40.0	24.0	41.0
47	34.49824797933617	37.0	33.0	40.0	24.0	41.0
48	34.40659004496297	37.0	33.0	40.0	24.0	41.0
49	34.29166972575037	36.0	33.0	40.0	24.0	41.0
50	34.11894607336016	36.0	33.0	40.0	24.0	41.0
51	32.354717996978735	35.0	30.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	5.0
8	51.0
9	70.0
10	93.0
11	52.0
12	42.0
13	35.0
14	55.0
15	65.0
16	153.0
17	252.0
18	546.0
19	1012.0
20	1545.0
21	2515.0
22	3645.0
23	5128.0
24	7096.0
25	9519.0
26	12041.0
27	13938.0
28	16419.0
29	19021.0
30	23211.0
31	28689.0
32	37102.0
33	50573.0
34	75807.0
35	80584.0
36	95498.0
37	134109.0
38	162182.0
39	117903.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.81496214522972	20.45737194675637	25.27826537717946	13.44940053083445
2	30.99574842985577	25.196615652274513	26.68099430231756	17.12664161555216
3	31.15927035848011	24.707718457509884	27.93788836458048	16.19512281942952
4	28.099407983874734	25.23732927531976	28.390855230810647	18.272407509994864
5	26.26151049766286	28.5523748501049	25.734903143848125	19.451211508384112
6	25.146780397836615	35.61540977260441	24.568335480253893	14.669474349305087
7	70.26626264513962	17.201728215430688	9.069905068289872	3.4621040711398257
8	83.06157546147668	5.535384143044979	7.8037781352270725	3.599262260251267
9	78.1069722635662	5.634275976070178	9.24822183807547	7.01052992228815
10	45.51215735481588	22.067451126966432	16.361314493827326	16.05907702439035
11	31.770642140602163	23.451158113468644	23.68487210805351	21.093327637875685
12	27.217724442189994	20.881972764143534	30.150328934927174	21.749973858739303
13	24.45431508784576	21.75920672953918	32.859119740322726	20.92735844229233
14	21.408246399736587	24.904167250673552	31.09519645991711	22.592389889672756
15	20.419216830077357	22.500951096931797	35.086355151830666	21.99347692116018
16	24.18055490665901	22.475032315047798	29.9652265613007	23.379186216992487
17	25.232212262587296	22.532876806806073	28.609996863048714	23.624914067557917
18	25.338000938860596	23.09852919255764	28.957286292412803	22.60618357616896
19	25.468818481760074	24.505040257541474	26.422918877549883	23.603222383148566
20	27.281242143716867	23.986108422825435	27.92287104460478	20.80977838885292
21	26.681328020539247	25.29372765478407	27.42084759978731	20.604096724889374
22	25.87117141770175	21.8614357447812	27.666464210945513	24.600928626571537
23	25.6383473383747	23.216776682440415	28.075491511320834	23.069384467864047
24	25.492734954313978	21.807929589904802	28.80499954391843	23.894335911862793
25	24.53140399705438	25.074029825509864	26.38476376087087	24.00980241656488
26	23.860741610880105	23.438254342230262	27.248204039770314	25.45280000711932
27	23.471626164398497	23.827703506933553	27.16800042715932	25.53266990150863
28	21.887799484294106	23.938275477717635	28.630576153385796	25.543348884602462
29	24.925413977453996	22.861144297534267	27.644216329500022	24.56922539551171
30	23.905126134363854	23.68175740465114	28.147129689575312	24.265986771409693
31	26.22713752082958	22.729325599969744	26.368077849786754	24.675459029413922
32	27.337195565552268	23.014209721879233	27.014601284592676	22.63399342797582
33	26.81192308462427	22.129745195013804	26.553068984006	24.50526273635593
34	25.27726422251441	22.683050006563125	28.727243198266443	23.31244257265602
35	23.573966419047746	24.198241972408177	28.62334559191601	23.604446016628067
36	25.68072955252836	25.096166467548127	26.733721781343373	22.48938219858014
37	23.853399810003094	24.914846233767392	27.698834878448697	23.532919077780818
38	24.063976007884648	25.33533119308714	26.653184450510697	23.947508348517513
39	24.69870806552446	23.88009726773768	28.685973378185064	22.735221288552797
40	26.053937763776446	23.232016481230573	28.384292105784226	22.329753649208754
41	22.276803691368492	24.21426044704893	29.22848796723332	24.280447894349262
42	22.76336485858134	23.577192361857342	29.282661558553087	24.37678122100823
43	23.241583070252137	23.02010541046229	28.014977273789103	25.72333424549647
44	22.979391787417043	22.475032315047798	28.681078844267056	25.864497053268103
45	24.02037016025149	22.290931096086375	27.558228267713208	26.13047047594893
46	25.22442550408137	23.42612924684247	27.8248691268374	23.52457612223876
47	21.867553912178714	23.03011695711276	30.826775770277276	24.275553360431253
48	23.201425644243027	23.696329766997938	28.61900725503414	24.483237333724897
49	23.388752806014047	22.47414239978998	30.639337369099028	23.497767425096946
50	22.695731298987056	22.9001893294711	29.691911337742866	24.71216803379898
51	22.61619512281943	22.759360239921154	28.446141216202687	26.178303421056732
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	236.0
1	623.0
2	1010.0
3	4300.0
4	7590.0
5	5337.5
6	3085.0
7	2853.5
8	2622.0
9	2531.0
10	2440.0
11	2428.0
12	2416.0
13	2349.0
14	2282.0
15	2206.0
16	2130.0
17	2024.0
18	1918.0
19	1998.5
20	2079.0
21	2239.5
22	2400.0
23	2978.0
24	3556.0
25	4105.0
26	5162.5
27	5671.0
28	6983.0
29	8295.0
30	9899.0
31	11503.0
32	13092.0
33	14681.0
34	18057.0
35	21433.0
36	22773.0
37	24113.0
38	26082.5
39	28052.0
40	30253.5
41	32455.0
42	36530.5
43	40606.0
44	44942.0
45	49278.0
46	63392.5
47	77507.0
48	69995.5
49	62484.0
50	62811.0
51	63138.0
52	59496.5
53	55855.0
54	53848.5
55	51842.0
56	52112.0
57	52382.0
58	52137.0
59	51892.0
60	51281.5
61	50671.0
62	48341.5
63	46012.0
64	41056.5
65	36101.0
66	31783.0
67	27465.0
68	22936.5
69	18408.0
70	15634.0
71	12860.0
72	10060.5
73	7261.0
74	6157.5
75	3949.0
76	2844.0
77	2062.5
78	1281.0
79	1031.0
80	781.0
81	549.5
82	318.0
83	225.5
84	133.0
85	96.5
86	60.0
87	54.0
88	48.0
89	35.0
90	22.0
91	22.5
92	23.0
93	16.0
94	9.0
95	7.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	898962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.713743118312237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.55664848397805	22.28486263062581
2	8.499525514107557	5.221044865356805
3	3.8973362668879843	3.5910535484063866
4	2.4727835155297946	3.037937507327167
5	1.6576359009056025	2.545610162205338
6	1.190049692301454	2.193052832842403
7	0.8995175727358745	1.9339286161592177
8	0.7088282325736572	1.7416614600219673
9	0.5662253253335998	1.5651809270441064
>10	7.378417792847522	48.9769916569161
>50	0.1475024473731111	2.7165776483307273
>100	0.02091980652947203	1.1528433252058468
>500	0.0021274379521493848	0.4201463771626464
>1k	0.0017728649601244874	1.4435044658607772
>5k	7.091459840497949E-4	1.175603976534673
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	5551	0.6174899495195569	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	5244	0.5833394515007309	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	4894	0.5444056589711245	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3685	0.4099172156331413	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT	1835	0.2041243122623648	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1834	0.20401307285513737	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCT	1007	0.11201808307803889	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2247881445489352E-4	0.0	0.0	0.07252809351229529	0.0
2	2.2247881445489352E-4	0.0	0.0	0.46776170739141365	0.0
3	2.2247881445489352E-4	0.0	0.0	0.5972443774041617	0.0
4	2.2247881445489352E-4	0.0	0.0	0.9817990081894452	0.0
5	2.2247881445489352E-4	0.0	0.0	2.1007562054903324	0.0
6	2.2247881445489352E-4	0.0	0.0	2.5114520969740655	0.0
7	2.2247881445489352E-4	0.0	0.0	2.943283475831014	0.0
8	2.2247881445489352E-4	0.0	0.0	3.5903631076730718	0.0
9	2.2247881445489352E-4	0.0	0.0	3.80783614880273	0.0
10	2.2247881445489352E-4	0.0	0.0	4.936137456310723	0.0
11	2.2247881445489352E-4	0.0	0.0	5.515361049744038	0.0
12	2.2247881445489352E-4	0.0	0.0	6.646220863618262	0.0
13	2.2247881445489352E-4	0.0	0.0	6.878266267094716	0.0
14	2.2247881445489352E-4	0.0	0.0	6.971039932722406	0.0
15	2.2247881445489352E-4	0.0	0.0	7.178390187794367	0.0
16	3.337182216823403E-4	0.0	0.0	7.481295093674705	0.0
17	3.337182216823403E-4	0.0	0.0	7.827694607780974	0.0
18	3.337182216823403E-4	0.0	0.0	8.23305100771779	0.0
19	3.337182216823403E-4	0.0	0.0	8.503140288466032	0.0
20	3.337182216823403E-4	0.0	0.0	8.746643350886911	0.0
21	3.337182216823403E-4	0.0	0.0	9.043652568184195	0.0
22	3.337182216823403E-4	0.0	0.0	9.377482029273763	0.0
23	3.337182216823403E-4	0.0	0.0	9.709865378069374	0.0
24	3.337182216823403E-4	0.0	0.0	9.988075135545218	0.0
25	3.337182216823403E-4	0.0	0.0	10.221344172501174	0.0
26	3.337182216823403E-4	0.0	0.0	10.45116478783308	0.0
27	4.4495762890978704E-4	0.0	0.0	10.689550837521496	0.0
28	4.4495762890978704E-4	0.0	0.0	10.930829111797829	0.0
29	4.4495762890978704E-4	0.0	0.0	11.17911546872949	0.0
30	4.4495762890978704E-4	0.0	0.0	11.463554633010071	0.0
31	4.4495762890978704E-4	0.0	0.0	11.717847917932016	0.0
32	4.4495762890978704E-4	0.0	0.0	11.974922188034645	0.0
33	4.4495762890978704E-4	0.0	0.0	12.227546881848175	0.0
34	4.4495762890978704E-4	0.0	0.0	12.495522613859094	0.0
35	5.561970361372339E-4	0.0	0.0	12.786302424351641	0.0
36	5.561970361372339E-4	0.0	0.0	13.04949486185178	0.0
37	5.561970361372339E-4	0.0	0.0	13.321586451930115	0.0
38	5.561970361372339E-4	0.0	0.0	13.638396283713883	0.0
39	5.561970361372339E-4	0.0	0.0	13.98835545885143	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCCG	20	7.032465E-4	45.0	1
GTAATCG	20	7.032465E-4	45.0	1
TATAGCG	75	0.0	45.0	1
CTCACGA	50	1.0804797E-9	40.5	24
TACGGCT	600	0.0	40.125	7
TCGAATG	45	1.927583E-8	40.000004	1
CGTTTTT	2080	0.0	39.69952	1
GCTATCG	30	1.1397239E-4	37.500004	20
ACGATAC	30	1.1397239E-4	37.500004	45
ATACGCG	30	1.1397239E-4	37.500004	1
CGGGATA	110	0.0	36.81818	6
ATAGCGG	215	0.0	36.627907	2
CGAGATA	235	0.0	36.38298	19
GCTACGA	130	0.0	36.346157	10
TACGAAT	125	0.0	36.000004	12
TCACGAC	50	4.8768925E-8	36.0	25
TATCGAC	25	0.0021068652	36.0	15
CTATGCG	45	8.697116E-7	35.000004	1
ACTACGG	45	8.697116E-7	35.000004	2
GTACGAG	45	8.697116E-7	35.000004	1
>>END_MODULE