FastQCFastQC Report
Sat 14 Jan 2017
SRR2934624.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934624.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903875
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50290.5563822431199004No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC26280.290748167611672No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG20010.22138016871801966No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC18790.20788272714700595No Hit
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC11060.12236205227492739No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG253.8901413E-545.0000041
TATCGTG207.032486E-445.01
CGCTTAC302.1649848E-644.99999618
CGTTTTT30400.042.18751
CACGACC4400.040.39772827
ACACGAC4500.039.526
GCTATCG403.457135E-739.3751
GCGAGAC4600.038.64130821
TCAAGCG4600.038.64130817
AACGTCG356.246808E-638.571431
ACGACCA4800.037.96874628
ACAACGA4500.037.513
CAAAACG301.139729E-437.4999961
GTCAAGC4900.037.1938816
CGAGACA4800.037.03124622
GACCGAT4450.036.9101149
TAGACGG1100.036.818182
AACACGT4550.036.59340741
CATGGTC4800.036.562512
GACACGA4900.036.2755125
Produced by FastQC (version 0.11.5)