##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934622.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 918962 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.113842574556944 31.0 31.0 33.0 28.0 34.0 2 31.18722101675586 31.0 31.0 34.0 28.0 34.0 3 30.845723762244795 31.0 30.0 34.0 27.0 34.0 4 34.78284956287638 35.0 35.0 37.0 32.0 37.0 5 35.090718658660535 35.0 35.0 37.0 33.0 37.0 6 35.071579673588246 36.0 35.0 37.0 32.0 37.0 7 31.11470876924182 35.0 35.0 37.0 0.0 37.0 8 33.081010966721145 36.0 35.0 37.0 17.0 37.0 9 36.39633521299031 39.0 35.0 39.0 32.0 39.0 10 36.135668286610326 37.0 35.0 39.0 32.0 39.0 11 36.37215575834474 38.0 35.0 39.0 32.0 39.0 12 36.55846270030752 38.0 35.0 39.0 32.0 39.0 13 36.598920303559886 39.0 35.0 39.0 32.0 39.0 14 37.76247113591204 39.0 37.0 41.0 33.0 41.0 15 37.84022625527497 40.0 37.0 41.0 33.0 41.0 16 37.78830245429082 39.0 37.0 41.0 33.0 41.0 17 37.593614317022904 39.0 36.0 41.0 32.0 41.0 18 37.43602020540566 39.0 36.0 40.0 32.0 41.0 19 37.169860124792976 39.0 36.0 40.0 32.0 41.0 20 37.05847793488741 39.0 35.0 40.0 32.0 41.0 21 37.156093505498596 39.0 35.0 40.0 32.0 41.0 22 37.22574926928426 39.0 35.0 40.0 32.0 41.0 23 37.28823716323417 39.0 35.0 40.0 32.0 41.0 24 37.18907963550179 39.0 35.0 40.0 32.0 41.0 25 36.91379621790673 39.0 35.0 40.0 31.0 41.0 26 36.88089823083 39.0 35.0 40.0 31.0 41.0 27 36.929425808684144 39.0 35.0 40.0 32.0 41.0 28 36.921299248499935 39.0 35.0 40.0 31.0 41.0 29 36.86555483251756 39.0 35.0 40.0 31.0 41.0 30 36.60516539312833 39.0 35.0 40.0 31.0 41.0 31 36.52741789105534 39.0 35.0 40.0 30.0 41.0 32 36.233401381123485 39.0 35.0 40.0 30.0 41.0 33 35.94898374470326 39.0 35.0 40.0 29.0 41.0 34 35.682838898670454 39.0 35.0 40.0 27.0 41.0 35 35.48043227032239 39.0 35.0 40.0 25.0 41.0 36 35.38521179330593 38.0 35.0 40.0 25.0 41.0 37 35.2330139875207 38.0 35.0 40.0 24.0 41.0 38 35.14728791832524 38.0 35.0 40.0 24.0 41.0 39 35.066615376914385 38.0 34.0 40.0 23.0 41.0 40 34.980666229942045 38.0 34.0 40.0 23.0 41.0 41 34.83219110257007 38.0 34.0 40.0 23.0 41.0 42 34.84688159031603 38.0 34.0 40.0 23.0 41.0 43 34.80480150430594 38.0 34.0 40.0 23.0 41.0 44 34.633968542768905 38.0 34.0 40.0 22.0 41.0 45 34.5785777866767 38.0 34.0 40.0 22.0 41.0 46 34.453794607393995 38.0 34.0 40.0 22.0 41.0 47 34.35380244232079 38.0 33.0 40.0 22.0 41.0 48 34.2945170746995 38.0 33.0 40.0 21.0 41.0 49 34.189494233711514 38.0 33.0 40.0 20.0 41.0 50 34.050421018496955 37.0 33.0 40.0 20.0 41.0 51 32.42672819986028 36.0 31.0 39.0 15.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 8.0 8 23.0 9 52.0 10 57.0 11 60.0 12 27.0 13 25.0 14 37.0 15 55.0 16 136.0 17 283.0 18 603.0 19 1102.0 20 1947.0 21 2753.0 22 4194.0 23 6075.0 24 9013.0 25 13053.0 26 17490.0 27 18908.0 28 18435.0 29 18757.0 30 21219.0 31 25657.0 32 32716.0 33 43165.0 34 61770.0 35 73614.0 36 89344.0 37 132364.0 38 178419.0 39 147588.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.35814320940365 18.83440229302191 23.18322193953613 17.624232558038308 2 35.342157782367494 25.059578089191938 23.979555193794738 15.618708934645827 3 29.062137498612568 25.132377617355235 30.898774922140415 14.906709961891787 4 25.486581599674413 23.260047749526098 34.068764540862404 17.184606109937082 5 25.810751695935195 27.06433998359018 29.412532835960576 17.71237548451405 6 23.339811657065255 33.91065136534481 29.143207227284694 13.606329750305235 7 66.5821872939251 15.873888147714489 14.553376526994589 2.9905480313658237 8 77.58188042595884 5.939527423331977 13.12099956254992 3.357592588159249 9 72.17665148286872 6.362722288843282 14.124414284812648 7.336211943475355 10 33.62739699791722 32.7514086545472 20.92175737408076 12.699436973454834 11 24.898744452980644 24.183154472110928 33.269602007482355 17.648499067426073 12 22.23062542303164 22.23138715202587 36.2087877409512 19.329199683991284 13 21.373245030806498 23.432960231217397 37.70406175663412 17.489732981341994 14 18.961828671914617 26.92494357764521 33.9213155712641 20.191912179176068 15 17.84665742435487 25.526844417941113 37.99177767959937 18.634720478104644 16 21.238854272537928 25.941769082943583 33.057188436518594 19.762188207999895 17 21.38369159987029 24.978834815803047 33.4956178819146 20.14185570241207 18 21.74094249816641 24.68720142943887 33.512049464504514 20.059806607890206 19 21.05854213775978 26.158970664728248 31.65060143945016 21.131885758061813 20 22.964932173473983 25.520968222842725 33.924688942524284 17.589410661159004 21 22.68646581686729 26.51263055490869 32.934332431591294 17.866571196632723 22 21.25125957330118 22.93663938225955 33.38538481460604 22.426716229833225 23 20.169169127776772 26.205653770232068 34.2391741987155 19.386002903275653 24 20.80619220381256 24.610484437876647 33.92349193981906 20.659831418491734 25 20.012905865530893 28.916429623858225 31.3856285678842 19.685035942726685 26 21.12198328113676 25.840676763565853 31.78695092941819 21.250389025879198 27 21.395552808494802 25.670920016279236 31.941255459964612 20.99227171526135 28 19.619962522933484 26.04014094162762 33.47483356221476 20.865062973224138 29 22.36425445230597 24.419181641895964 32.32016122538255 20.896402680415513 30 22.046831098565555 24.417658183907495 34.23438618789461 19.301124529632347 31 23.733734365512394 25.555898938149785 30.6241172105049 20.08624948583293 32 23.31554514767749 27.103841072862643 30.934140911158458 18.64647286830141 33 23.289646361873505 25.373845708527664 30.163271168992843 21.173236760605988 34 22.815959745887206 27.04039992948566 30.945675664499728 19.197964660127404 35 21.23722199612171 26.05918416648349 32.538668628300194 20.16492520909461 36 25.221826364963952 27.323219023202267 28.704342508177703 18.75061210365608 37 21.65475830339013 28.176137859889742 31.084745615161452 19.084358221558674 38 23.301181115214774 26.94409562092883 29.357905985231163 20.39681727862523 39 23.17157836776711 27.798973189315774 28.956583623697174 20.072864819219944 40 23.096602471048858 26.140036258300125 31.930700072473073 18.832661198177945 41 21.636259170672997 28.03347690111234 29.735070655805135 20.595193272409524 42 21.52243509524877 26.302937444638623 31.834939856054984 20.339687604057623 43 23.716541053928236 25.812275153923665 29.065293233017254 21.405890559130846 44 22.207882371632344 26.16549977039312 29.719509620637197 21.907108237337344 45 21.623962688337496 25.5022514532701 29.10152976945728 23.772256088935126 46 23.0091124551396 27.225717711940213 28.961480452945825 20.80368937997436 47 21.119262820443065 25.789314465668873 32.76033176562252 20.331090948265544 48 21.64463818960958 26.363984582605156 30.02855395544103 21.96282327234423 49 21.40425828271463 25.07187457152744 32.258461176849536 21.2654059689084 50 21.089664208095655 25.612049246867663 31.579543006130827 21.71874353890585 51 21.177807134571395 24.903858919084794 29.863149945264333 24.05518400107948 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 245.0 1 606.0 2 967.0 3 10917.0 4 20867.0 5 14659.5 6 8452.0 7 7803.0 8 7154.0 9 6941.5 10 6729.0 11 6582.0 12 6435.0 13 6172.5 14 5910.0 15 5619.5 16 5329.0 17 5128.0 18 4927.0 19 4764.5 20 4602.0 21 4773.5 22 4945.0 23 5180.0 24 5415.0 25 6506.5 26 8815.0 27 10032.0 28 11386.5 29 12741.0 30 15444.0 31 18147.0 32 20147.5 33 22148.0 34 26267.5 35 30387.0 36 32317.5 37 34248.0 38 36303.5 39 38359.0 40 41564.5 41 44770.0 42 48141.5 43 51513.0 44 56380.0 45 61247.0 46 81065.5 47 100884.0 48 86081.5 49 71279.0 50 69821.0 51 68363.0 52 61546.5 53 54730.0 54 50180.0 55 45630.0 56 42210.0 57 38790.0 58 35261.0 59 31732.0 60 29526.5 61 27321.0 62 24874.5 63 22428.0 64 18922.0 65 15416.0 66 12347.5 67 9279.0 68 8221.0 69 7163.0 70 5861.0 71 4559.0 72 3981.0 73 3403.0 74 2760.0 75 1782.5 76 1448.0 77 992.0 78 536.0 79 423.0 80 310.0 81 247.0 82 184.0 83 127.5 84 71.0 85 68.5 86 66.0 87 62.5 88 59.0 89 38.5 90 18.0 91 11.5 92 5.0 93 2.5 94 0.0 95 1.5 96 3.0 97 1.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 918962.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.259391218991546 #Duplication Level Percentage of deduplicated Percentage of total 1 73.40632001898803 25.882621554959893 2 9.170416733231466 6.466866224763894 3 4.147780798904678 4.3874467763760405 4 2.468127942878685 3.480987548659374 5 1.6389870600084888 2.8894842975852035 6 1.0964011528381878 2.3195062308525 7 0.910066502914604 2.246187360309587 8 0.7062254169945906 1.9920862613286165 9 0.6055320669610753 1.9215622840162623 >10 5.73114319505154 39.47657786656419 >50 0.09423291557451731 2.0408386530791054 >100 0.019329713681262146 1.2633241176064844 >500 0.002416214210132031 0.6408736862268278 >1k 0.0018121606575990234 1.1998544072312511 >5k 6.040535525330077E-4 1.4638500649508102 >10k+ 6.040535525330077E-4 2.3279326654899397 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11346 1.2346538812268624 No Hit GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC 10514 1.144116949340669 RNA PCR Primer, Index 17 (95% over 21bp) GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG 7176 0.7808810375184174 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC 6570 0.7149370703032334 No Hit GAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT 3397 0.3696561990593735 TruSeq Adapter, Index 9 (95% over 21bp) GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC 2505 0.27259016150831045 RNA PCR Primer, Index 47 (95% over 23bp) GAATGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTT 1541 0.16768919715940378 No Hit GAATGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCT 1431 0.15571917010714262 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTC 1343 0.14614314846533372 No Hit GAATCTATCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC 1050 0.11425934913521996 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 924 0.10054822723899355 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.1763685549565706E-4 0.0 0.0 0.07813163112294089 0.0 2 2.1763685549565706E-4 0.0 0.0 0.501326496634246 0.0 3 2.1763685549565706E-4 0.0 0.0 0.6856649132390675 0.0 4 2.1763685549565706E-4 0.0 0.0 1.23878898148128 0.0 5 2.1763685549565706E-4 0.0 0.0 2.8420108774900377 0.0 6 2.1763685549565706E-4 0.0 0.0 3.48131914050853 0.0 7 2.1763685549565706E-4 0.0 0.0 3.988630650668907 0.0 8 2.1763685549565706E-4 0.0 0.0 4.734363336024776 0.0 9 2.1763685549565706E-4 0.0 0.0 5.0466722236610435 0.0 10 2.1763685549565706E-4 0.0 0.0 6.3990676437110565 0.0 11 2.1763685549565706E-4 0.0 0.0 6.906596790726929 0.0 12 2.1763685549565706E-4 0.0 0.0 8.173787381850392 0.0 13 2.1763685549565706E-4 0.0 0.0 8.46052393896592 0.0 14 2.1763685549565706E-4 0.0 0.0 8.631477688957759 0.0 15 2.1763685549565706E-4 0.0 0.0 8.881324799066773 0.0 16 2.1763685549565706E-4 0.0 0.0 9.160335247812206 0.0 17 3.264552832434856E-4 0.0 0.0 9.481240791240552 0.0 18 3.264552832434856E-4 0.0 0.0 9.830439125883334 0.0 19 3.264552832434856E-4 0.0 0.0 10.089318165495417 0.0 20 3.264552832434856E-4 0.0 0.0 10.317401590054866 0.0 21 3.264552832434856E-4 0.0 0.0 10.573451350545508 0.0 22 3.264552832434856E-4 0.0 0.0 10.882930959060332 0.0 23 3.264552832434856E-4 0.0 0.0 11.17967881152866 0.0 24 4.3527371099131413E-4 0.0 0.0 11.41918817100163 0.0 25 4.3527371099131413E-4 0.0 0.0 11.638457302913505 0.0 26 4.3527371099131413E-4 0.0 0.0 11.847279865761587 0.0 27 4.3527371099131413E-4 0.0 0.0 12.08265412498014 0.0 28 4.3527371099131413E-4 0.0 0.0 12.31324037337779 0.0 29 4.3527371099131413E-4 0.0 0.0 12.553511461844995 0.0 30 4.3527371099131413E-4 0.0 0.0 12.863317525643062 0.0 31 4.3527371099131413E-4 0.0 0.0 13.122087746827399 0.0 32 4.3527371099131413E-4 0.0 0.0 13.411218309353378 0.0 33 4.3527371099131413E-4 0.0 0.0 13.667703343555011 0.0 34 5.440921387391426E-4 0.0 0.0 13.917441635236278 0.0 35 5.440921387391426E-4 0.0 0.0 14.196778539264953 0.0 36 5.440921387391426E-4 0.0 0.0 14.449454928495411 0.0 37 6.529105664869712E-4 0.0 0.0 14.710183881379209 0.0 38 6.529105664869712E-4 0.0 0.0 14.999640899188432 0.0 39 6.529105664869712E-4 0.0 0.0 15.313364426385421 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATACG 20 7.032548E-4 45.0 10 CGTTTTT 6825 0.0 43.516487 1 TACGGCT 890 0.0 41.713486 7 CGTTTTA 420 0.0 40.714287 1 ACGGCTG 1035 0.0 39.782604 8 ACCGCGC 40 3.4572076E-7 39.375 35 TATTGCG 35 6.2469207E-6 38.57143 1 CGAATAT 175 0.0 38.57143 14 GCTACGA 170 0.0 38.38235 10 CACGACC 300 0.0 38.25 27 TGATACC 940 0.0 38.05851 4 CGGCTGT 1090 0.0 37.77523 9 AACACGT 300 0.0 37.5 41 CGGTTTA 60 1.546141E-10 37.499996 1 TTACGTA 30 1.139744E-4 37.499996 45 CGGTCTA 30 1.139744E-4 37.499996 31 ACACGAC 310 0.0 37.01613 26 GACACGA 310 0.0 37.01613 25 ACGACCA 310 0.0 37.01613 28 TTGGACG 55 2.748493E-9 36.81818 1 >>END_MODULE