SRR2934620.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934620.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	806641
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	9727	1.2058648147069142	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG	9262	1.148218352402122	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	7810	0.9682126249471574	RNA PCR Primer, Index 40 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5320	0.6595251171214952	No Hit
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	2668	0.3307543256541634	Illumina PCR Primer Index 8 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	1805	0.22376745045193588	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCT	1707	0.21161830355759254	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTC	1388	0.17207159070763822	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTA	1213	0.15037668553916797	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	932	0.11554086638293863	RNA PCR Primer, Index 40 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATA	20	7.0320343E-4	45.0	27
GACCGAA	20	7.0320343E-4	45.0	32
CCGTCTA	20	7.0320343E-4	45.0	30
TACGCTA	25	3.8897677E-5	45.0	1
TCGTCGT	20	7.0320343E-4	45.0	26
CGCACCT	20	7.0320343E-4	45.0	33
ATCGGTT	20	7.0320343E-4	45.0	11
GACGATA	20	7.0320343E-4	45.0	8
TATACGG	45	3.8380676E-10	45.0	2
CGTTTTT	3355	0.0	41.84799	1
CTAACGG	70	0.0	41.785713	2
TATAGCG	55	6.002665E-11	40.909092	1
TACGGCT	1045	0.0	40.47847	7
CTACGAA	90	0.0	40.0	11
TACGAAT	90	0.0	40.0	12
CGAATAT	90	0.0	40.0	14
ACGGCTG	1185	0.0	39.873417	8
CACGGGA	225	0.0	39.0	4
ACGGGTA	35	6.245973E-6	38.571426	5
TTACGCG	35	6.245973E-6	38.571426	1