##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934620.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	806641
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.099761851926694	31.0	31.0	33.0	28.0	34.0
2	31.15332347351548	31.0	31.0	34.0	28.0	34.0
3	30.897402933895005	31.0	31.0	34.0	27.0	34.0
4	34.87958211893519	35.0	35.0	37.0	32.0	37.0
5	35.12337706612979	35.0	35.0	37.0	33.0	37.0
6	35.082311709918045	36.0	35.0	37.0	32.0	37.0
7	31.193433014190948	35.0	35.0	37.0	0.0	37.0
8	33.07725245803275	35.0	35.0	37.0	17.0	37.0
9	36.27108713789654	39.0	35.0	39.0	32.0	39.0
10	36.047459774546546	37.0	35.0	39.0	32.0	39.0
11	36.27523396405588	37.0	35.0	39.0	32.0	39.0
12	36.31274011611113	38.0	35.0	39.0	32.0	39.0
13	36.1643841560248	38.0	35.0	39.0	32.0	39.0
14	37.17755730244309	39.0	36.0	40.0	32.0	41.0
15	37.29087413111905	39.0	36.0	41.0	32.0	41.0
16	37.35446623714887	39.0	36.0	40.0	32.0	41.0
17	37.2077045426652	39.0	36.0	40.0	32.0	41.0
18	37.12411717232325	39.0	36.0	40.0	32.0	41.0
19	36.870120660864	39.0	35.0	40.0	31.0	41.0
20	36.78990901776627	39.0	35.0	40.0	31.0	41.0
21	36.898591814698236	39.0	35.0	40.0	31.0	41.0
22	37.00771966711338	39.0	35.0	40.0	32.0	41.0
23	37.065309102810296	39.0	35.0	40.0	32.0	41.0
24	36.96164960620648	39.0	35.0	40.0	32.0	41.0
25	36.695027899648046	38.0	35.0	40.0	31.0	41.0
26	36.628519998363586	38.0	35.0	40.0	31.0	41.0
27	36.67786165096989	38.0	35.0	40.0	31.0	41.0
28	36.67867985882195	39.0	35.0	40.0	31.0	41.0
29	36.61717790194151	39.0	35.0	40.0	31.0	41.0
30	36.39756223648438	38.0	35.0	40.0	30.0	41.0
31	36.367912119517854	38.0	35.0	40.0	30.0	41.0
32	36.16905289961706	38.0	35.0	40.0	30.0	41.0
33	35.94200393979478	38.0	35.0	40.0	29.0	41.0
34	35.80720791529317	38.0	35.0	40.0	29.0	41.0
35	35.73062242063074	38.0	35.0	40.0	28.0	41.0
36	35.638898840996184	38.0	35.0	40.0	28.0	41.0
37	35.55423044452241	38.0	35.0	40.0	27.0	41.0
38	35.311494208700026	38.0	34.0	40.0	26.0	41.0
39	35.189451069310884	38.0	34.0	40.0	25.0	41.0
40	35.01197682736186	38.0	34.0	40.0	25.0	41.0
41	34.890283285873146	38.0	34.0	40.0	24.0	41.0
42	34.87886432750133	38.0	34.0	40.0	24.0	41.0
43	34.83362859066177	38.0	34.0	40.0	24.0	41.0
44	34.613498446024934	38.0	34.0	40.0	23.0	41.0
45	34.49534303364198	37.0	33.0	40.0	23.0	41.0
46	34.3075519345037	37.0	33.0	40.0	23.0	41.0
47	34.171078583905356	37.0	33.0	40.0	23.0	41.0
48	34.14398970545757	37.0	33.0	40.0	23.0	41.0
49	34.041557520631855	37.0	33.0	40.0	23.0	41.0
50	33.89487888664226	36.0	33.0	40.0	22.0	41.0
51	32.08603703506269	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	24.0
9	52.0
10	55.0
11	40.0
12	28.0
13	22.0
14	45.0
15	79.0
16	150.0
17	258.0
18	552.0
19	1024.0
20	1650.0
21	2484.0
22	3785.0
23	5121.0
24	7029.0
25	9587.0
26	12420.0
27	14112.0
28	15189.0
29	17389.0
30	20657.0
31	25939.0
32	33388.0
33	45253.0
34	65518.0
35	71241.0
36	81707.0
37	115393.0
38	145382.0
39	111058.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.472346434163406	18.452322656547338	22.25773299398369	12.817597915305571
2	34.01661953706792	26.230380057547286	23.159869136332023	16.593131269052776
3	30.443902553924236	26.775480046265937	26.94804752052028	15.832569879289549
4	26.315424085807688	24.508052528944102	30.98181223121562	18.194711154032586
5	27.633854465617297	27.333968890745698	25.910535169920696	19.121641473716313
6	24.392015779014457	36.596701630589074	25.27555628836124	13.73572630203523
7	67.00006570456993	16.39713329721648	13.129260724411479	3.4735402738021004
8	77.23609387571423	8.022527989526939	10.768607100308564	3.9727710344502696
9	72.15080810422481	6.249000484726167	12.171213712171834	9.428977698877196
10	40.68513750230896	24.82145093046349	19.571283879693695	14.922127687533859
11	32.91042731524929	24.843393777405314	24.608221005379097	17.637957901966303
12	27.441699591267987	22.887877010962747	29.694498543961934	19.97592485380733
13	24.046756859619087	24.369701019412602	33.7706612979008	17.812880823067513
14	21.62225822887753	26.604648164425065	31.12313904202737	20.649954564670033
15	19.923733110516327	23.768814131689314	37.32515456070297	18.982298197091396
16	23.860924500490306	24.524292715098785	29.462053131442612	22.152729652968297
17	24.51102783022435	24.127213965072443	29.92322482988095	21.438533374822256
18	26.51427338803755	22.544229713094175	30.352164097783273	20.589332801084993
19	24.67615705127808	25.2870855808222	28.32821540189502	21.708541966004706
20	26.15736120529455	25.817433034026287	30.027980229122992	17.997225531556168
21	24.53470626957965	28.089323503268492	29.475441987203727	17.90052823994813
22	23.948943830030956	22.250790624329785	30.669281625902972	23.130983919736288
23	22.131778573119888	27.01077678917883	30.398901122060494	20.458543515640788
24	22.750889181184693	24.78797879106071	29.515980467147095	22.945151560607506
25	21.6644083303477	29.10662859933973	28.151432917493658	21.077530152818913
26	21.251461306826705	25.421470021980035	29.496641008825485	23.83042766236777
27	22.793039282654863	25.69544568153615	28.60293488677119	22.9085801490378
28	19.263588138961445	26.52183561212485	31.206943361421004	23.0076328874927
29	22.993500206411525	27.02850462597364	27.8744819566573	22.10351321095754
30	22.416291758043542	26.3051345022135	29.632637071510125	21.64593666823283
31	24.1764304070832	26.77957108552628	26.13355879505257	22.910439712337954
32	25.861566669683288	26.906269331710142	26.660806976089734	20.571357022516832
33	23.167679302192674	26.19814762701127	25.580772611359947	25.053400459436105
34	21.773626681510112	27.264297252433238	28.98166594556934	21.980410120487303
35	20.79834771602237	26.981271718149706	28.31185124485366	23.90852932097426
36	22.789692068714583	29.985086302332757	25.011870212399316	22.213351416553337
37	22.61117399189974	27.38343327452981	26.80275364133487	23.20263909223558
38	21.708417995118026	29.788096563403048	25.93706493966957	22.566420501809358
39	24.242258947908674	25.542837520036795	25.750240813447373	24.464662718607162
40	23.680670831261985	26.01715013246289	29.006584093791414	21.295594942483707
41	20.92479802043288	25.847557959488793	26.16951035218889	27.058133667889432
42	21.870571914891507	24.62532898774052	30.530682174598116	22.973416922769857
43	22.60559530199928	24.929305601872457	26.83387033389079	25.631228762237473
44	22.92333268455236	24.93203296137935	28.077670239920856	24.066964114147435
45	21.815032957660225	24.800747792388435	27.208014469881892	26.17620478006945
46	23.565253935765725	26.770149298138822	26.53522446788596	23.12937229820949
47	19.96117231829277	25.56874743535228	31.839938708793625	22.630141537561318
48	22.354802198251765	26.863499375806587	27.073753999610734	23.707944426330922
49	22.8315942284114	23.994441145441407	30.33406434832844	22.839900277818757
50	21.53671831707042	25.285597930182075	28.45144246325193	24.726241289495572
51	21.719451404032277	25.020176261806675	26.230504028433966	27.029868305727085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	153.0
1	605.0
2	1057.0
3	5577.5
4	10098.0
5	6971.5
6	3845.0
7	3646.0
8	3447.0
9	3385.5
10	3324.0
11	3321.5
12	3319.0
13	3381.0
14	3443.0
15	3338.0
16	3233.0
17	3057.5
18	2882.0
19	3074.5
20	3267.0
21	3128.0
22	2989.0
23	3396.0
24	3803.0
25	4478.0
26	5670.5
27	6188.0
28	7063.5
29	7939.0
30	9140.5
31	10342.0
32	12318.0
33	14294.0
34	17189.5
35	20085.0
36	21478.5
37	22872.0
38	25623.0
39	28374.0
40	31155.0
41	33936.0
42	37369.5
43	40803.0
44	45290.5
45	49778.0
46	71213.5
47	92649.0
48	78360.5
49	64072.0
50	65070.0
51	66068.0
52	60462.0
53	54856.0
54	51672.0
55	48488.0
56	45181.0
57	41874.0
58	39052.0
59	36230.0
60	33239.5
61	30249.0
62	27582.0
63	24915.0
64	22278.5
65	19642.0
66	17139.5
67	14637.0
68	12088.5
69	9540.0
70	8169.0
71	6798.0
72	5680.5
73	4563.0
74	3946.5
75	2704.5
76	2079.0
77	1451.5
78	824.0
79	704.0
80	584.0
81	438.5
82	293.0
83	219.0
84	145.0
85	121.0
86	97.0
87	66.5
88	36.0
89	27.5
90	19.0
91	15.0
92	11.0
93	12.0
94	13.0
95	8.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	806641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.508131655317477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.66554309314527	23.84079893549919
2	8.372034069960705	5.275743033982506
3	3.56696004032558	3.3716473967950473
4	2.053023141780484	2.58747693770532
5	1.384954161760496	2.1818659032664782
6	1.0431482634123599	1.9720591691767462
7	0.7431693027772577	1.6391113363867436
8	0.5910697113024369	1.4898801824950139
9	0.47025646627029155	1.333521238590784
>10	5.647588699892957	41.08356363376085
>50	0.42940257915867563	8.289943615750552
>100	0.027766468870908934	1.5605947798194948
>500	0.0015643081054033201	0.334052305415764
>1k	0.00195538513175415	1.0820041659372273
>5k	0.0015643081054033201	3.957737365418267
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	9727	1.2058648147069142	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG	9262	1.148218352402122	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	7810	0.9682126249471574	RNA PCR Primer, Index 40 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5320	0.6595251171214952	No Hit
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	2668	0.3307543256541634	Illumina PCR Primer Index 8 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	1805	0.22376745045193588	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCT	1707	0.21161830355759254	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTC	1388	0.17207159070763822	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTA	1213	0.15037668553916797	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	932	0.11554086638293863	RNA PCR Primer, Index 40 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2397088667697278E-4	0.0	0.0	0.07388664845947578	0.0
2	1.2397088667697278E-4	0.0	0.0	0.5982834991030707	0.0
3	1.2397088667697278E-4	0.0	0.0	0.742089727648359	0.0
4	1.2397088667697278E-4	0.0	0.0	1.1650783929901902	0.0
5	1.2397088667697278E-4	0.0	0.0	2.7043009219714844	0.0
6	1.2397088667697278E-4	0.0	0.0	3.172910873610441	0.0
7	1.2397088667697278E-4	0.0	0.0	3.7222258724761077	0.0
8	1.2397088667697278E-4	0.0	0.0	4.529028402969846	0.0
9	1.2397088667697278E-4	0.0	0.0	4.802508178979249	0.0
10	1.2397088667697278E-4	0.0	0.0	6.680295199475355	0.0
11	1.2397088667697278E-4	0.0	0.0	7.357176240731627	0.0
12	1.2397088667697278E-4	0.0	0.0	9.277361304471258	0.0
13	2.4794177335394556E-4	0.0	0.0	9.592371327517446	0.0
14	2.4794177335394556E-4	0.0	0.0	9.75849231566459	0.0
15	2.4794177335394556E-4	0.0	0.0	10.09668489451937	0.0
16	2.4794177335394556E-4	0.0	0.0	10.49029245971876	0.0
17	2.4794177335394556E-4	0.0	0.0	10.927041893481734	0.0
18	2.4794177335394556E-4	0.0	0.0	11.407677021128357	0.0
19	2.4794177335394556E-4	0.0	0.0	11.751076377223573	0.0
20	2.4794177335394556E-4	0.0	0.0	12.058276234409112	0.0
21	2.4794177335394556E-4	0.0	0.0	12.411097377891776	0.0
22	2.4794177335394556E-4	0.0	0.0	12.800613903830824	0.0
23	2.4794177335394556E-4	0.0	0.0	13.185791448736179	0.0
24	2.4794177335394556E-4	0.0	0.0	13.490263946414824	0.0
25	2.4794177335394556E-4	0.0	0.0	13.762999897104164	0.0
26	2.4794177335394556E-4	0.0	0.0	14.035859818680182	0.0
27	2.4794177335394556E-4	0.0	0.0	14.365250464580898	0.0
28	2.4794177335394556E-4	0.0	0.0	14.635383026650022	0.0
29	2.4794177335394556E-4	0.0	0.0	14.950517020582886	0.0
30	2.4794177335394556E-4	0.0	0.0	15.336686332581657	0.0
31	2.4794177335394556E-4	0.0	0.0	15.648473112574242	0.0
32	2.4794177335394556E-4	0.0	0.0	15.961747543206954	0.0
33	2.4794177335394556E-4	0.0	0.0	16.274030206746247	0.0
34	2.4794177335394556E-4	0.0	0.0	16.586932724718928	0.0
35	2.4794177335394556E-4	0.0	0.0	16.941737402388423	0.0
36	2.4794177335394556E-4	0.0	0.0	17.242614744353435	0.0
37	2.4794177335394556E-4	0.0	0.0	17.555517262326116	0.0
38	2.4794177335394556E-4	0.0	0.0	17.942554370531624	0.0
39	2.4794177335394556E-4	0.0	0.0	18.5208785568797	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATA	20	7.0320343E-4	45.0	27
GACCGAA	20	7.0320343E-4	45.0	32
CCGTCTA	20	7.0320343E-4	45.0	30
TACGCTA	25	3.8897677E-5	45.0	1
TCGTCGT	20	7.0320343E-4	45.0	26
CGCACCT	20	7.0320343E-4	45.0	33
ATCGGTT	20	7.0320343E-4	45.0	11
GACGATA	20	7.0320343E-4	45.0	8
TATACGG	45	3.8380676E-10	45.0	2
CGTTTTT	3355	0.0	41.84799	1
CTAACGG	70	0.0	41.785713	2
TATAGCG	55	6.002665E-11	40.909092	1
TACGGCT	1045	0.0	40.47847	7
CTACGAA	90	0.0	40.0	11
TACGAAT	90	0.0	40.0	12
CGAATAT	90	0.0	40.0	14
ACGGCTG	1185	0.0	39.873417	8
CACGGGA	225	0.0	39.0	4
ACGGGTA	35	6.245973E-6	38.571426	5
TTACGCG	35	6.245973E-6	38.571426	1
>>END_MODULE