##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934619.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	821625
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.150096455195495	31.0	31.0	33.0	28.0	34.0
2	31.206438460368172	31.0	31.0	34.0	28.0	34.0
3	30.949518332572644	31.0	31.0	34.0	27.0	34.0
4	34.89195557584056	35.0	35.0	37.0	32.0	37.0
5	35.14221816522136	35.0	35.0	37.0	33.0	37.0
6	35.09254830366651	36.0	35.0	37.0	32.0	37.0
7	30.88510330138445	35.0	35.0	37.0	0.0	37.0
8	32.91081576144835	35.0	35.0	37.0	17.0	37.0
9	36.309559713981436	39.0	35.0	39.0	32.0	39.0
10	36.12944713220752	37.0	35.0	39.0	32.0	39.0
11	36.3317571884984	38.0	35.0	39.0	32.0	39.0
12	36.398802373345504	38.0	35.0	39.0	32.0	39.0
13	36.362054465236575	38.0	35.0	39.0	32.0	39.0
14	37.444261068005474	39.0	36.0	41.0	32.0	41.0
15	37.570728738779856	39.0	36.0	41.0	32.0	41.0
16	37.530290582686746	39.0	36.0	41.0	32.0	41.0
17	37.40871443785182	39.0	36.0	41.0	32.0	41.0
18	37.28760079111517	39.0	36.0	40.0	32.0	41.0
19	37.02494081849992	39.0	36.0	40.0	31.0	41.0
20	36.965811653734974	39.0	35.0	40.0	31.0	41.0
21	37.034956336528225	39.0	35.0	40.0	32.0	41.0
22	37.13004229423399	39.0	35.0	40.0	32.0	41.0
23	37.183140727217406	39.0	35.0	40.0	32.0	41.0
24	37.06847649475125	39.0	35.0	40.0	32.0	41.0
25	36.76867914194432	39.0	35.0	40.0	31.0	41.0
26	36.69034413509813	38.0	35.0	40.0	31.0	41.0
27	36.72498767685988	39.0	35.0	40.0	31.0	41.0
28	36.68466879659212	39.0	35.0	40.0	31.0	41.0
29	36.63535797961357	39.0	35.0	40.0	31.0	41.0
30	36.385002890613116	38.0	35.0	40.0	30.0	41.0
31	36.3786715350677	38.0	35.0	40.0	30.0	41.0
32	36.15313068614027	38.0	35.0	40.0	30.0	41.0
33	35.92818621633957	38.0	35.0	40.0	29.0	41.0
34	35.7436969420356	38.0	35.0	40.0	28.0	41.0
35	35.60877285866423	38.0	35.0	40.0	27.0	41.0
36	35.50653156853796	38.0	35.0	40.0	27.0	41.0
37	35.43583630001521	38.0	35.0	40.0	26.0	41.0
38	35.231016582991025	38.0	34.0	40.0	25.0	41.0
39	35.13091495511943	38.0	34.0	40.0	25.0	41.0
40	34.93866666666667	38.0	34.0	40.0	24.0	41.0
41	34.76991449870683	38.0	34.0	40.0	23.0	41.0
42	34.780404685836	38.0	34.0	40.0	24.0	41.0
43	34.70967290430549	38.0	34.0	40.0	23.0	41.0
44	34.529012627415185	37.0	34.0	40.0	23.0	41.0
45	34.41516750342309	37.0	33.0	40.0	23.0	41.0
46	34.27392180130838	37.0	33.0	40.0	23.0	41.0
47	34.1428230640499	37.0	33.0	40.0	23.0	41.0
48	34.07777514072722	37.0	33.0	40.0	23.0	41.0
49	33.954117754450024	36.0	33.0	40.0	23.0	41.0
50	33.79542126882702	36.0	33.0	40.0	22.0	41.0
51	32.068402251635476	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	29.0
9	64.0
10	68.0
11	51.0
12	41.0
13	39.0
14	46.0
15	85.0
16	169.0
17	320.0
18	583.0
19	986.0
20	1701.0
21	2664.0
22	3614.0
23	5201.0
24	7144.0
25	10284.0
26	13370.0
27	15057.0
28	15900.0
29	17604.0
30	20709.0
31	25495.0
32	32519.0
33	43882.0
34	63791.0
35	71311.0
36	84889.0
37	120239.0
38	149749.0
39	114014.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49733759318424	18.524752776509963	22.737867031796743	15.240042598509051
2	33.525270044119885	25.30972158831584	24.1246310664841	17.040377301080177
3	30.647558192606116	25.0296668188042	28.979522288148484	15.343252700441198
4	26.568933515898372	24.353445915107258	30.62370302753689	18.453917541457475
5	25.838186520614638	28.422942339875245	26.84764947512551	18.891221664384602
6	24.75837517115472	34.79933059485775	26.37054617374106	14.071748060246463
7	67.93330290582686	16.978305187889852	11.778852883006238	3.3095390232770425
8	79.41457477559713	5.783903849079568	11.090095846645367	3.711425528677925
9	74.27622090369694	5.8076373041229274	12.170394036208734	7.745747755971398
10	41.46064202038643	24.404929256047467	18.916415639738325	15.218013083827781
11	31.10543130990415	24.169785486079416	25.710025863380498	19.014757340635935
12	25.89392971246006	21.876403468735734	31.340575079872206	20.889091738931995
13	23.64898828541001	23.079141944317662	33.573467214361784	19.698402555910544
14	20.859516202647193	26.086596683401797	31.93342461585273	21.12046249809828
15	19.553689335158982	22.909599878289974	35.827780313403316	21.708930473147724
16	23.398509052183172	23.537136771641563	30.98323444393732	22.081119732237944
17	23.655560626806633	23.38901567016583	30.638186520614635	22.317237182412903
18	25.048166742735432	22.83535676251331	30.122622851057358	21.9938536436939
19	23.9531416400426	25.311182108626195	27.565860337745324	23.169815913585882
20	26.55165069222577	24.806937471474214	29.549003499163245	19.09240833713677
21	25.488878746386735	25.235113342461585	29.113038186520612	20.162969724631065
22	24.77870074547391	21.21442263806481	29.848288452761295	24.158588163699985
23	24.562178609462954	24.779309295603223	29.8728738779857	20.785638216948122
24	24.125483036665145	23.511942796287844	29.820660276890308	22.5419138901567
25	23.668705309599876	26.662650235813175	27.860155180282973	21.808489274303973
26	23.499041533546325	23.801856077894417	28.536497794005783	24.16260459455348
27	24.356610375779706	23.849688118058726	28.61865206146356	23.175049444698008
28	21.305583447436483	25.039281910847404	29.72572645671687	23.92940818499924
29	23.877924844059027	25.555940970637458	28.143130990415337	22.42300319488818
30	25.02528525787312	23.79735280693747	29.850600943252704	21.32676099193671
31	26.303118819412752	23.568294538262588	26.813570667883766	23.315015974440893
32	26.403407880724174	24.515685379583143	28.020203864293318	21.06070287539936
33	24.59418834626502	23.84092499619656	27.75694507835083	23.807941579187585
34	23.35019017191541	25.53731933668036	28.854404381560933	22.258086109843298
35	24.443754754297885	25.967929408184997	28.201064962726303	21.387250874790812
36	23.720675490643544	29.128251939753536	25.969633348547088	21.181439221055832
37	24.714194431766316	26.302266849231703	26.737745321770877	22.245793397231097
38	23.371976266544955	26.670561387494296	27.021573102084282	22.935889243876463
39	24.9296211775445	24.955058572949948	26.150981287083525	23.96433896242203
40	24.467853339418834	23.853947968963944	30.05592575688422	21.622272934732997
41	21.88261068005477	26.780100410771336	26.97276738171307	24.364521527460823
42	23.357736193518942	25.5099345808611	29.02674577818348	22.105583447436484
43	24.23173588924388	24.36500836756428	27.577422790202345	23.825832952989504
44	23.816461280998023	24.17392362695877	27.718241290126272	24.291373801916933
45	23.427719458390385	23.509021755667124	27.457660124752774	25.605598661189717
46	24.462376388254985	24.831888026776205	26.736406511486383	23.969329073482427
47	21.815791875855773	25.478898524265936	30.407180891525936	22.29812870835235
48	22.82987981134946	25.71854556519093	28.010588772250117	23.440985851209494
49	23.426258938080025	24.06012475277651	30.272082762817586	22.24153354632588
50	22.54970333181196	24.87241746538871	29.32554389167808	23.252335311121254
51	22.531568537958314	23.92003651300776	27.75524113798874	25.793153811045183
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	264.0
1	710.5
2	1157.0
3	7450.5
4	13744.0
5	9432.5
6	5121.0
7	4710.5
8	4300.0
9	4233.0
10	4166.0
11	4105.5
12	4045.0
13	3873.5
14	3702.0
15	3500.0
16	3298.0
17	3154.5
18	3011.0
19	2992.5
20	2974.0
21	3161.0
22	3348.0
23	3413.5
24	3479.0
25	3877.0
26	5212.5
27	6150.0
28	7467.0
29	8784.0
30	10259.5
31	11735.0
32	13090.5
33	14446.0
34	16481.0
35	18516.0
36	20657.0
37	22798.0
38	24349.5
39	25901.0
40	27487.0
41	29073.0
42	31878.5
43	34684.0
44	39652.5
45	44621.0
46	60417.0
47	76213.0
48	66411.5
49	56610.0
50	57668.0
51	58726.0
52	55720.0
53	52714.0
54	50904.5
55	49095.0
56	48081.5
57	47068.0
58	45993.5
59	44919.0
60	42852.5
61	40786.0
62	36857.5
63	32929.0
64	29522.0
65	26115.0
66	22516.0
67	18917.0
68	16935.5
69	14954.0
70	12921.0
71	10888.0
72	9186.0
73	7484.0
74	6317.5
75	3981.0
76	2811.0
77	2007.0
78	1203.0
79	998.5
80	794.0
81	545.5
82	297.0
83	231.0
84	165.0
85	125.5
86	86.0
87	73.0
88	60.0
89	41.5
90	23.0
91	19.5
92	16.0
93	11.5
94	7.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	821625.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.737246903200734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.7963562306264	24.222113969027973
2	8.974678939327859	6.055619185061193
3	4.180193903397521	4.230847014665298
4	2.5775378309117207	3.4783612081523674
5	1.7532464209672325	2.9574853693162266
6	1.296269268203822	2.62395338126542
7	1.000448541300847	2.3626665616269587
8	0.8337457760623876	2.2502629681213984
9	0.7662143428712098	2.3264966219597736
>10	6.727866978226844	42.208203191473935
>50	0.0615866297107285	1.309110215565097
>100	0.027607786607670836	1.681315749016902
>500	0.0014157839285982336	0.33483021024452186
>1k	0.001769729910747792	1.4061913537230706
>5k	0.0010618379464486752	2.5525430007799215
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	8409	1.0234596074851665	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7474	0.9096607333029059	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	5493	0.668553172067549	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	4698	0.5717937015061615	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT	2616	0.3183934276586034	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	2045	0.24889700289061312	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTT	1336	0.16260459455347634	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT	1081	0.1315685379583143	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC	991	0.12061463563061006	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.43420051726761E-4	0.0	0.0	0.060489882854100105	0.0
2	2.43420051726761E-4	0.0	0.0	0.43085349155636693	0.0
3	2.43420051726761E-4	0.0	0.0	0.5615700593336376	0.0
4	2.43420051726761E-4	0.0	0.0	1.0375779704853187	0.0
5	2.43420051726761E-4	0.0	0.0	2.4503879507074395	0.0
6	2.43420051726761E-4	0.0	0.0	2.966195040316446	0.0
7	2.43420051726761E-4	0.0	0.0	3.407637304122927	0.0
8	2.43420051726761E-4	0.0	0.0	4.067183934276586	0.0
9	2.43420051726761E-4	0.0	0.0	4.369511638521224	0.0
10	2.43420051726761E-4	0.0	0.0	5.567503423094477	0.0
11	2.43420051726761E-4	0.0	0.0	6.036573862771946	0.0
12	2.43420051726761E-4	0.0	0.0	7.050540088239769	0.0
13	2.43420051726761E-4	0.0	0.0	7.264019473604138	0.0
14	2.43420051726761E-4	0.0	0.0	7.386703179674425	0.0
15	2.43420051726761E-4	0.0	0.0	7.577422790202343	0.0
16	2.43420051726761E-4	0.0	0.0	7.7977179370150616	0.0
17	2.43420051726761E-4	0.0	0.0	8.050509660733303	0.0
18	2.43420051726761E-4	0.0	0.0	8.31717632739997	0.0
19	2.43420051726761E-4	0.0	0.0	8.511912368781378	1.217100258633805E-4
20	2.43420051726761E-4	0.0	0.0	8.694233987524722	1.217100258633805E-4
21	2.43420051726761E-4	0.0	0.0	8.90114103149247	1.217100258633805E-4
22	2.43420051726761E-4	0.0	0.0	9.14310056290887	1.217100258633805E-4
23	2.43420051726761E-4	0.0	0.0	9.383234443937319	1.217100258633805E-4
24	2.43420051726761E-4	0.0	0.0	9.580039555758406	1.217100258633805E-4
25	2.43420051726761E-4	0.0	0.0	9.74726913129469	1.217100258633805E-4
26	2.43420051726761E-4	0.0	0.0	9.904883614787769	1.217100258633805E-4
27	2.43420051726761E-4	0.0	0.0	10.110086718393427	1.217100258633805E-4
28	2.43420051726761E-4	0.0	0.0	10.288635326335006	1.217100258633805E-4
29	2.43420051726761E-4	0.0	0.0	10.47205233531112	1.217100258633805E-4
30	2.43420051726761E-4	0.0	0.0	10.730199300167351	1.217100258633805E-4
31	3.651300775901415E-4	0.0	0.0	10.931629392971246	1.217100258633805E-4
32	3.651300775901415E-4	0.0	0.0	11.159957401490948	1.217100258633805E-4
33	3.651300775901415E-4	0.0	0.0	11.365038795070744	1.217100258633805E-4
34	3.651300775901415E-4	0.0	0.0	11.576083979917845	1.217100258633805E-4
35	3.651300775901415E-4	0.0	0.0	11.805385668644455	1.217100258633805E-4
36	3.651300775901415E-4	0.0	0.0	12.013631522896699	1.217100258633805E-4
37	3.651300775901415E-4	0.0	0.0	12.237943100562909	1.217100258633805E-4
38	3.651300775901415E-4	0.0	0.0	12.49146508443633	1.217100258633805E-4
39	3.651300775901415E-4	0.0	0.0	12.875460216035297	1.217100258633805E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTG	20	7.03211E-4	45.000004	17
TGTAACG	20	7.03211E-4	45.000004	1
TAATCGT	40	6.8102963E-9	45.000004	21
GCGAGAC	335	0.0	41.641792	21
CGTTTTT	4085	0.0	41.640152	1
CGTTTTA	290	0.0	41.120693	1
ACACGAC	335	0.0	40.97015	26
GTATGCG	55	6.002665E-11	40.909092	1
TTAATCG	45	1.9272193E-8	40.0	20
GACACGA	345	0.0	39.782608	25
AAACACG	345	0.0	39.130436	40
CACGTGA	340	0.0	39.044117	43
AACACGT	340	0.0	39.044117	41
TACGGCT	555	0.0	38.51351	7
CTATCTC	255	0.0	37.941177	5
AGACACG	370	0.0	37.7027	24
CACGACC	365	0.0	37.60274	27
ACGACCA	365	0.0	37.60274	28
GAATCTG	1140	0.0	37.105263	1
ACACGTG	360	0.0	36.875	42
>>END_MODULE