##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934618.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1013973
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178630989188076	31.0	31.0	33.0	28.0	34.0
2	31.2361246305375	31.0	31.0	34.0	28.0	34.0
3	31.009013060505556	31.0	31.0	34.0	28.0	34.0
4	34.992772983107045	35.0	35.0	37.0	32.0	37.0
5	35.225304815808705	35.0	35.0	37.0	33.0	37.0
6	35.198587141866696	36.0	35.0	37.0	32.0	37.0
7	31.276129640532833	35.0	35.0	37.0	0.0	37.0
8	33.21775431890198	36.0	35.0	37.0	17.0	37.0
9	36.54379554485178	39.0	35.0	39.0	32.0	39.0
10	36.14253929838368	37.0	35.0	39.0	32.0	39.0
11	36.39600166868349	38.0	35.0	39.0	32.0	39.0
12	36.41608898856281	38.0	35.0	39.0	32.0	39.0
13	36.38439386453091	38.0	35.0	39.0	32.0	39.0
14	37.33286685148421	39.0	36.0	40.0	32.0	41.0
15	37.53912579526279	39.0	36.0	41.0	32.0	41.0
16	37.48117849291845	39.0	36.0	41.0	32.0	41.0
17	37.395973068316415	39.0	36.0	40.0	32.0	41.0
18	37.29701185337282	39.0	36.0	40.0	32.0	41.0
19	37.06065940611831	39.0	35.0	40.0	32.0	41.0
20	37.055154328566935	39.0	35.0	40.0	32.0	41.0
21	37.04538483766333	39.0	35.0	40.0	31.0	41.0
22	37.164862377992314	39.0	35.0	40.0	32.0	41.0
23	37.205671156924296	39.0	35.0	40.0	32.0	41.0
24	37.13618508579617	39.0	35.0	40.0	32.0	41.0
25	36.807583633883745	39.0	35.0	40.0	31.0	41.0
26	36.78936520005957	39.0	35.0	40.0	31.0	41.0
27	36.801460196671904	39.0	35.0	40.0	31.0	41.0
28	36.80428867435326	39.0	35.0	40.0	31.0	41.0
29	36.768248266965685	39.0	35.0	40.0	31.0	41.0
30	36.49385437284819	38.0	35.0	40.0	30.0	41.0
31	36.46301528738931	38.0	35.0	40.0	30.0	41.0
32	36.324501737225745	38.0	35.0	40.0	30.0	41.0
33	36.183834283555875	38.0	35.0	40.0	30.0	41.0
34	36.07208574587292	38.0	35.0	40.0	30.0	41.0
35	35.95039710130349	38.0	35.0	40.0	29.0	41.0
36	35.87099459255818	38.0	35.0	40.0	29.0	41.0
37	35.78305339491288	38.0	35.0	40.0	29.0	41.0
38	35.54872269774442	38.0	34.0	40.0	27.0	41.0
39	35.47023540074539	38.0	34.0	40.0	27.0	41.0
40	35.24640794182883	38.0	34.0	40.0	26.0	41.0
41	35.05354481825453	38.0	34.0	40.0	26.0	41.0
42	35.10608665122247	38.0	34.0	40.0	26.0	41.0
43	35.08160473700976	38.0	34.0	40.0	26.0	41.0
44	34.88853943842686	38.0	34.0	40.0	25.0	41.0
45	34.78593118357195	37.0	34.0	40.0	25.0	41.0
46	34.6606319892147	37.0	34.0	40.0	24.0	41.0
47	34.53823523900538	37.0	33.0	40.0	24.0	41.0
48	34.46435654598298	37.0	33.0	40.0	24.0	41.0
49	34.34853788019996	36.0	33.0	40.0	24.0	41.0
50	34.190828552634045	36.0	33.0	40.0	24.0	41.0
51	32.48666285985919	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	32.0
9	82.0
10	86.0
11	80.0
12	38.0
13	34.0
14	52.0
15	78.0
16	174.0
17	333.0
18	612.0
19	1084.0
20	1711.0
21	2708.0
22	3941.0
23	5670.0
24	7998.0
25	10776.0
26	13613.0
27	15752.0
28	17744.0
29	20166.0
30	24813.0
31	30688.0
32	39466.0
33	54118.0
34	81028.0
35	88916.0
36	104533.0
37	152021.0
38	193115.0
39	142497.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16966329478201	20.73082813842183	24.642668000035503	12.456840566760654
2	33.86017181917072	23.170538071526558	25.487463670137174	17.48182643916554
3	32.770103345947085	23.294505869485675	27.019358503628794	16.91603228093845
4	27.89630493119639	26.131169173143665	26.46165134574589	19.51087454991405
5	25.57730827152202	29.819630305737928	25.709658935691582	18.893402487048473
6	26.12061662391405	34.80773156681687	24.436745357124895	14.63490645214419
7	72.5229370012811	16.59038258415165	7.846165529062412	3.0405148855048405
8	84.27916719675967	4.885041317668222	7.538662272072333	3.297129213499768
9	79.12873419706442	6.037833354537054	9.399362704924096	5.434069743474431
10	42.99325524446903	25.95591795836773	16.801630812654775	14.249195984508464
11	33.5628266235886	25.717252826258687	21.95778388576422	18.7621366643885
12	27.646002408348153	24.390984769811425	27.260193318757008	20.70281950308342
13	26.571023094303296	24.61584282816209	29.780871877259056	19.03226220027555
14	22.869741107504833	27.306348393892144	29.47179066898231	20.35211982962071
15	21.421872180028462	26.341332560137204	31.86317584393273	20.373619415901604
16	26.987109124207446	25.15767185122286	27.985459178893322	19.869759845676363
17	27.035236638450925	24.667224866934326	26.8589005821654	21.438637912449344
18	27.762376315740163	23.21886282968087	27.13583103297622	21.882929821602744
19	27.271633465585378	25.084494360303477	25.13479155756613	22.509080616545017
20	28.717036844176324	26.76067311457011	25.485491231028835	19.03679881022473
21	26.057301328536365	27.02724826006215	26.51973967748648	20.395710733915006
22	26.081463707613516	22.530087093048827	27.311969845350916	24.076479353986745
23	25.6493022989764	25.699993984060722	27.379032775034446	21.271670941928434
24	24.912103182234635	23.752802096308283	28.275111861952933	23.05998285950415
25	23.917796627720854	25.474642815932967	26.313422546754207	24.29413800959197
26	23.69205097177144	24.44197232076199	27.012750832615858	24.85322587485071
27	23.112745605652222	24.34709799965088	28.359039146012766	24.181117248684135
28	21.21269501258909	27.32459345564428	27.45368959528508	24.009021936481542
29	24.10419212345891	26.690552904268657	26.364705963571023	22.840549008701416
30	25.24564263545479	25.275031978169043	27.523316695809452	21.956008690566712
31	25.045538687913783	26.105527464735257	26.190539590304674	22.65839425704629
32	25.427896009065332	24.845040252551104	26.741441833263806	22.98562190511976
33	24.39670484322561	24.954313379153092	26.896672791090097	23.7523089865312
34	22.886210974059466	26.141425856507027	28.428469002626304	22.543894166807203
35	22.512335141073773	25.906212492837582	28.027570753856363	23.553881612232278
36	23.90112951725539	28.876705789996382	26.09103003728896	21.13113465545927
37	24.016024095316148	27.56207512428832	25.745853193329605	22.676047587065927
38	25.39850666635108	28.166726332949693	24.517812604477633	21.9169543962216
39	25.390518287962305	25.83352811169528	24.845927850149856	23.93002575019256
40	25.95335378752689	24.6246201821942	27.791864280409833	21.630161749869078
41	22.215976165045813	26.17239315050795	26.935036731747296	24.676593952698937
42	23.017180930853186	25.978403764202795	28.559044471598355	22.44537083334566
43	22.847847033402267	24.794348567466788	28.81220703115369	23.54559736797725
44	23.221624244432544	24.552034423007317	28.926509877481944	23.299831455078195
45	23.790377061322147	24.438027442545316	26.695188136173254	25.07640735995929
46	25.17887557163751	25.206588341109676	25.59939958953542	24.015136497717396
47	22.5485293987118	27.43948803370504	27.084547616159405	22.927434951423756
48	22.09417805010587	27.02823447961632	26.625561035648875	24.252026434628927
49	23.389380190596793	25.23075072018683	26.891544449408418	24.488324639807963
50	21.541500611949232	26.437784832534987	26.776551249392245	25.244163306123536
51	21.53321636769421	26.09625700092606	25.469514474251287	26.901012157128445
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	190.0
1	639.0
2	1088.0
3	4864.0
4	8640.0
5	5950.5
6	3261.0
7	3147.0
8	3033.0
9	2927.5
10	2822.0
11	2825.5
12	2829.0
13	2837.5
14	2846.0
15	2737.0
16	2628.0
17	2582.0
18	2536.0
19	2661.0
20	2786.0
21	3063.0
22	3340.0
23	3486.5
24	3633.0
25	4410.5
26	6060.0
27	6932.0
28	8450.0
29	9968.0
30	11863.5
31	13759.0
32	15547.5
33	17336.0
34	20680.0
35	24024.0
36	25708.0
37	27392.0
38	30478.5
39	33565.0
40	37061.5
41	40558.0
42	43779.0
43	47000.0
44	51620.0
45	56240.0
46	65143.0
47	74046.0
48	73421.5
49	72797.0
50	77966.5
51	83136.0
52	83459.0
53	83782.0
54	80325.0
55	76868.0
56	71095.0
57	65322.0
58	59625.5
59	53929.0
60	49269.5
61	44610.0
62	40535.5
63	36461.0
64	32613.0
65	28765.0
66	24867.5
67	20970.0
68	18628.5
69	16287.0
70	14227.5
71	12168.0
72	10497.0
73	8826.0
74	7382.0
75	4719.5
76	3501.0
77	3059.0
78	2617.0
79	1884.0
80	1151.0
81	902.5
82	654.0
83	496.0
84	338.0
85	223.0
86	108.0
87	79.0
88	50.0
89	40.5
90	31.0
91	25.5
92	20.0
93	11.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1013973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.298563570554553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9804194292864	21.675200216252367
2	8.439322657474339	4.945200627448665
3	3.647459486713138	3.2059597092746155
4	2.2429782106890626	2.6286415877296876
5	1.5799256469565228	2.3144776002052607
6	1.154268339508745	2.0291042593545248
7	0.8596732400859364	1.7631033752193694
8	0.6487350726748874	1.5205604613770813
9	0.5320913581663392	1.4030561234321282
>10	6.279733039709439	44.71759474621534
>50	0.598095137422311	10.698802722424562
>100	0.035317936287480124	1.8661548013574183
>500	3.3007417091055066E-4	0.07543145281948269
>1k	0.0016503708545527532	1.1567123168895288
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4495	0.44330568959923	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	2267	0.2235759729302457	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	2068	0.20395020380227086	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	1973	0.19458111803765976	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1158	0.11420422437283832	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.862195541695883E-5	0.0	0.0	0.050494441173482923	0.0
2	9.862195541695883E-5	0.0	0.0	0.29280858563295076	0.0
3	9.862195541695883E-5	0.0	0.0	0.38659806523447865	0.0
4	9.862195541695883E-5	0.0	0.0	0.5809819393613045	0.0
5	9.862195541695883E-5	0.0	0.0	1.1755737085701492	0.0
6	9.862195541695883E-5	0.0	0.0	1.540474943612897	0.0
7	9.862195541695883E-5	0.0	0.0	1.892653946406857	0.0
8	9.862195541695883E-5	0.0	0.0	2.6026334034535434	0.0
9	9.862195541695883E-5	0.0	0.0	2.9218726731382394	0.0
10	9.862195541695883E-5	0.0	0.0	3.6971398646709526	0.0
11	9.862195541695883E-5	0.0	0.0	4.296662731650645	0.0
12	9.862195541695883E-5	0.0	0.0	5.03977916571743	0.0
13	9.862195541695883E-5	0.0	0.0	5.281008468667312	0.0
14	9.862195541695883E-5	0.0	0.0	5.388900887893465	0.0
15	9.862195541695883E-5	0.0	0.0	5.577663310561523	0.0
16	9.862195541695883E-5	0.0	0.0	5.86544217646821	0.0
17	9.862195541695883E-5	0.0	0.0	6.212985947357573	0.0
18	9.862195541695883E-5	0.0	0.0	6.601457829744973	0.0
19	9.862195541695883E-5	0.0	0.0	6.855310742988226	0.0
20	9.862195541695883E-5	0.0	0.0	7.104232558460629	0.0
21	9.862195541695883E-5	0.0	0.0	7.392405912188983	0.0
22	9.862195541695883E-5	0.0	0.0	7.721310133504541	0.0
23	9.862195541695883E-5	0.0	0.0	8.019641548640841	0.0
24	9.862195541695883E-5	0.0	0.0	8.271916510597423	0.0
25	9.862195541695883E-5	0.0	0.0	8.505256057113947	0.0
26	1.9724391083391767E-4	0.0	0.0	8.728043054400857	0.0
27	1.9724391083391767E-4	0.0	0.0	8.98574222390537	0.0
28	1.9724391083391767E-4	0.0	0.0	9.225492197523998	0.0
29	1.9724391083391767E-4	0.0	0.0	9.484078964627264	0.0
30	1.9724391083391767E-4	0.0	0.0	9.840794577370403	0.0
31	1.9724391083391767E-4	0.0	0.0	10.102241381180761	0.0
32	1.9724391083391767E-4	0.0	0.0	10.397811381565386	0.0
33	1.9724391083391767E-4	0.0	0.0	10.686576467026242	0.0
34	1.9724391083391767E-4	0.0	0.0	10.966268332588738	0.0
35	2.958658662508765E-4	0.0	0.0	11.259767271909608	0.0
36	2.958658662508765E-4	0.0	0.0	11.54212193026836	0.0
37	2.958658662508765E-4	0.0	0.0	11.827632491200456	0.0
38	2.958658662508765E-4	0.0	0.0	12.180797713548586	0.0
39	2.958658662508765E-4	0.0	0.0	12.74008282271816	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	20	7.032893E-4	45.0	1
TCGATTG	25	3.890479E-5	45.0	1
GTAATCG	20	7.032893E-4	45.0	1
ACCCGTG	25	3.890479E-5	45.0	37
GATTACG	20	7.032893E-4	45.0	1
TACGTGC	30	2.1652486E-6	44.999996	16
CGTTTTT	2555	0.0	41.38943	1
TACGGGT	80	0.0	39.375	4
ACGGGTA	80	0.0	39.375	5
CTAACGG	40	3.4576806E-7	39.375	2
AACACGT	515	0.0	38.883495	41
CACAACG	530	0.0	38.632076	12
TAACGAC	35	6.247559E-6	38.57143	21
CTTTCGT	35	6.247559E-6	38.57143	15
AGCGACT	465	0.0	38.225807	19
CACGACC	525	0.0	38.142857	27
TTACCGG	65	9.094947E-12	38.076927	2
TACGGCT	320	0.0	37.96875	7
CGGATTA	30	1.1398272E-4	37.499996	42
GACGATT	30	1.1398272E-4	37.499996	38
>>END_MODULE