##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934617.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	540246
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00037760575738	33.0	31.0	34.0	30.0	34.0
2	32.19765255087497	33.0	31.0	34.0	30.0	34.0
3	32.04368565431304	33.0	31.0	34.0	30.0	34.0
4	35.81898061253577	37.0	35.0	37.0	35.0	37.0
5	32.152286180739885	37.0	35.0	37.0	10.0	37.0
6	34.007640963561045	37.0	35.0	37.0	19.0	37.0
7	20.649898379627057	32.0	0.0	37.0	0.0	37.0
8	28.03622979161345	35.0	17.0	37.0	17.0	37.0
9	34.83351103016034	37.0	32.0	39.0	32.0	39.0
10	36.26656560159631	37.0	35.0	39.0	32.0	39.0
11	36.73424328916827	39.0	37.0	39.0	33.0	39.0
12	37.07859382577566	39.0	37.0	39.0	34.0	39.0
13	36.82994598756863	39.0	37.0	39.0	33.0	39.0
14	38.239564938935224	40.0	38.0	41.0	34.0	41.0
15	38.425831935821826	40.0	38.0	41.0	34.0	41.0
16	38.50505695553507	40.0	38.0	41.0	34.0	41.0
17	38.478226585666526	40.0	38.0	41.0	34.0	41.0
18	38.38560211459224	40.0	38.0	41.0	34.0	41.0
19	38.32303802341896	40.0	37.0	41.0	34.0	41.0
20	38.29351443601619	40.0	37.0	41.0	34.0	41.0
21	38.21241064255913	40.0	37.0	41.0	34.0	41.0
22	38.167673615353	40.0	37.0	41.0	34.0	41.0
23	38.11004431314623	40.0	37.0	41.0	34.0	41.0
24	37.98802582527219	40.0	36.0	41.0	33.0	41.0
25	37.84918907312595	40.0	36.0	41.0	33.0	41.0
26	37.72634688641841	40.0	36.0	41.0	33.0	41.0
27	37.68559322975089	40.0	36.0	41.0	33.0	41.0
28	37.62679038808247	40.0	36.0	41.0	33.0	41.0
29	37.63438692743676	40.0	36.0	41.0	33.0	41.0
30	37.49030626788537	40.0	36.0	41.0	33.0	41.0
31	37.35238206298612	40.0	36.0	41.0	32.0	41.0
32	37.26429071200897	40.0	36.0	41.0	32.0	41.0
33	37.119795426527915	40.0	35.0	41.0	32.0	41.0
34	36.97765462400462	40.0	35.0	41.0	31.0	41.0
35	36.866747740843984	40.0	35.0	41.0	31.0	41.0
36	36.68666126172151	39.0	35.0	41.0	30.0	41.0
37	36.62926888861741	39.0	35.0	41.0	30.0	41.0
38	36.52193445208294	39.0	35.0	41.0	30.0	41.0
39	36.462896532320464	39.0	35.0	41.0	30.0	41.0
40	36.340837322256895	39.0	35.0	41.0	30.0	41.0
41	36.246458095015974	39.0	35.0	40.0	30.0	41.0
42	36.1633478082207	39.0	35.0	40.0	29.0	41.0
43	36.063633974152516	39.0	35.0	40.0	29.0	41.0
44	35.94123047648664	39.0	35.0	40.0	29.0	41.0
45	35.84381004209194	39.0	35.0	40.0	28.0	41.0
46	35.692838077468416	38.0	35.0	40.0	27.0	41.0
47	35.65179011043117	38.0	35.0	40.0	27.0	41.0
48	35.541190494700565	38.0	35.0	40.0	27.0	41.0
49	35.40254809845885	38.0	34.0	40.0	27.0	41.0
50	35.27470078445745	38.0	34.0	40.0	26.0	41.0
51	33.37366310902811	36.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	24.0
10	42.0
11	20.0
12	16.0
13	8.0
14	14.0
15	17.0
16	46.0
17	87.0
18	153.0
19	282.0
20	564.0
21	954.0
22	1553.0
23	2319.0
24	3387.0
25	4712.0
26	5661.0
27	6386.0
28	7100.0
29	8403.0
30	10276.0
31	13366.0
32	17737.0
33	25816.0
34	36485.0
35	47061.0
36	61519.0
37	104642.0
38	110057.0
39	71513.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.61639697471152	21.26253595584234	25.790658329723865	13.330408739722275
2	32.14091358381182	24.837573994069366	25.701254613638973	17.32025780847984
3	31.5326721530562	23.993514065814463	28.19956094075662	16.274252840372718
4	28.292481573209244	26.127912099302907	26.775950215272303	18.803656112215545
5	23.036542612069315	37.45515931631146	22.4273756770064	17.080922394612823
6	26.985299289582894	34.821359158605524	24.522347227003994	13.670994324807587
7	49.44839943285096	43.27972812385469	4.787078479063242	2.4847939642311094
8	85.52326162525961	4.944784413026658	7.021986280324148	2.5099676813895893
9	78.93755807539529	8.827830284722145	8.285484760646076	3.949126879236496
10	37.65303213721157	35.624326695616446	14.731992462692922	11.99064870447907
11	32.150723929469166	25.453959862729203	24.781118231324246	17.61419797647738
12	31.600233967488883	22.330382825601667	27.43250297086883	18.636880236040618
13	25.37047937421101	26.26229532472244	27.51283674474221	20.85438855632434
14	19.584226444989874	28.76060165184009	28.12274408325096	23.532427819919075
15	19.820230043350623	27.667581064922274	31.91897765092199	20.593211240805115
16	22.966204284714742	25.65627510430434	31.36460057085106	20.012920040129867
17	22.31113233600989	25.4302669524624	28.711735024414804	23.54686568711291
18	23.18517860382122	26.423703275915045	29.099706430033724	21.291411690230007
19	24.312998152693403	27.788822129178186	26.74152145504085	21.156658263087557
20	26.660632378583088	25.747159627280908	28.836863206761365	18.75534478737464
21	26.10033207094546	26.450913102549578	27.10894666503778	20.339808161467182
22	25.039333933060128	25.29144130636784	27.306264183353505	22.362960577218526
23	23.538351047485776	27.066743668625033	28.24990837507358	21.144996908815614
24	21.99442476205284	25.501345683262812	31.00698570651148	21.49724384817287
25	22.18970617089252	27.763278210296793	27.980956823373056	22.066058795437634
26	21.830240297938346	29.193552566793645	26.946798310399338	22.02940882486867
27	21.266423073932987	28.5425528370409	28.714696638198156	21.476327450827956
28	19.88057292418639	29.01437493290094	29.48434602014638	21.620706122766293
29	21.019683625607595	26.27747359536211	30.243814854714334	22.45902792431596
30	22.04273608689375	27.467301932823197	29.08212184819508	21.407840132087973
31	23.110027654068702	28.52126623797307	27.222783694835318	21.14592241312291
32	23.271065403538387	28.26379093968303	27.439351702742826	21.025791954035753
33	22.857735179899528	28.68489539950319	26.76132724721701	21.696042173380274
34	21.280490739403902	28.203633159708723	28.245651055260012	22.270225045627363
35	22.836078379108777	26.353364948560472	27.96985077168549	22.84070590064526
36	22.610255328128297	29.485086423592215	26.753923212758636	21.150735035520853
37	22.06365248423866	28.757825138918196	28.373000447944086	20.805521928899058
38	21.24939379467872	27.729219651788263	28.673789347815624	22.347597205717395
39	22.0133050499217	27.311632108335832	28.248797769904822	22.426265071837644
40	23.522987675984645	27.295528333388862	28.007796448284672	21.17368754234182
41	20.34776749850994	26.911258944999126	30.032059469204768	22.70891408728616
42	21.542408458368964	27.321257353131724	27.998541405211704	23.137792783287615
43	22.23376017591986	27.482850405185786	28.393916845288995	21.889472573605357
44	21.51575393431881	27.851941522935846	27.5667010954269	23.065603447318445
45	21.342499527992803	28.62899493934245	26.16567267504063	23.86283285762412
46	21.02597705489721	29.692399388426754	26.16548757417917	23.116135982496864
47	21.43856687509024	28.26971416724973	29.13320968595788	21.15850927170215
48	21.54648067732107	26.857394594314442	29.19262706248635	22.403497665878135
49	20.875119852807796	27.460268100087738	29.056948131036602	22.607663916067867
50	20.333329631316104	27.548561211003875	28.904795222916967	23.213313934763054
51	20.341659170081776	26.843326928843524	28.018717399110777	24.796296501963923
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	433.0
1	989.0
2	1545.0
3	3631.0
4	5717.0
5	4045.0
6	2373.0
7	2122.0
8	1871.0
9	1831.5
10	1792.0
11	1813.0
12	1834.0
13	1819.0
14	1804.0
15	1795.0
16	1786.0
17	1803.0
18	1820.0
19	1778.5
20	1737.0
21	2019.5
22	2302.0
23	2688.0
24	3074.0
25	3805.5
26	5485.0
27	6433.0
28	7628.0
29	8823.0
30	10586.5
31	12350.0
32	14310.0
33	16270.0
34	17598.0
35	18926.0
36	20091.5
37	21257.0
38	23242.0
39	25227.0
40	27382.0
41	29537.0
42	31501.0
43	33465.0
44	35397.5
45	37330.0
46	38253.0
47	39176.0
48	38470.5
49	37765.0
50	36527.0
51	35289.0
52	33204.5
53	31120.0
54	29470.0
55	27820.0
56	27083.0
57	26346.0
58	25398.5
59	24451.0
60	23636.5
61	22822.0
62	20486.0
63	18150.0
64	15152.5
65	12155.0
66	10121.5
67	8088.0
68	6716.0
69	5344.0
70	4367.0
71	3390.0
72	2953.0
73	2516.0
74	2092.0
75	1314.0
76	960.0
77	712.5
78	465.0
79	358.0
80	251.0
81	184.0
82	117.0
83	85.0
84	53.0
85	34.0
86	15.0
87	16.0
88	17.0
89	15.0
90	13.0
91	10.0
92	7.0
93	5.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	540246.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.362425903532085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.41681592262269	24.16000655388222
2	7.043242109084483	4.699592859699362
3	3.3041722091349515	3.307056014993242
4	2.326415493995836	3.1045945815706024
5	1.7465143471080173	2.9133977747423474
6	1.445171739716405	2.892866105050027
7	1.2044837571897777	2.812915007087633
8	1.0783531295081557	2.8781181104846225
9	0.9342937891527754	2.8053276581465725
>10	8.427600882993426	47.35955216373433
>50	0.05105563364544871	1.1075452267895871
>100	0.019636782171326087	1.1335632741993658
>500	0.0011221018383614908	0.28432671953580524
>1k	0.0011221018383614908	0.5411379500842745
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1872	0.3465088126520141	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1019	0.188617777827138	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	839	0.15529962276444434	No Hit
GATACTAAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	680	0.1258685857923983	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01962069131469738	0.0
2	0.0	0.0	0.0	0.10421178500164739	0.0
3	0.0	0.0	0.0	0.1423425624622857	0.0
4	0.0	0.0	0.0	0.18473065973649042	0.0
5	0.0	0.0	0.0	0.3265179196143979	0.0
6	0.0	0.0	0.0	0.44646327784009504	0.0
7	0.0	0.0	0.0	0.5619662153907664	0.0
8	0.0	0.0	0.0	0.8394324067184209	0.0
9	0.0	0.0	0.0	0.9479015115336347	0.0
10	0.0	0.0	0.0	1.2744194311480326	0.0
11	0.0	0.0	0.0	1.7634559071237919	0.0
12	0.0	0.0	0.0	2.130881117120719	0.0
13	0.0	0.0	0.0	2.291918866590405	0.0
14	0.0	0.0	0.0	2.355778663793901	0.0
15	0.0	0.0	0.0	2.491827796966567	0.0
16	0.0	0.0	0.0	2.7533753142087125	0.0
17	0.0	0.0	0.0	3.0715636950574368	0.0
18	0.0	0.0	0.0	3.4008581275937257	0.0
19	0.0	0.0	0.0	3.604098873476157	0.0
20	0.0	0.0	0.0	3.8114118383106956	0.0
21	0.0	0.0	0.0	4.079252784842461	0.0
22	0.0	0.0	0.0	4.387445719172377	0.0
23	0.0	0.0	0.0	4.713593437063856	0.0
24	0.0	0.0	0.0	4.952373548346494	0.0
25	0.0	0.0	0.0	5.178751901911352	0.0
26	0.0	0.0	0.0	5.3755141176427035	0.0
27	0.0	0.0	0.0	5.577459157494919	0.0
28	0.0	0.0	0.0	5.801801401583723	0.0
29	0.0	0.0	0.0	6.040026210281983	0.0
30	0.0	0.0	0.0	6.2982419120178585	0.0
31	0.0	0.0	0.0	6.5479429741266015	0.0
32	0.0	0.0	0.0	6.78320616904151	0.0
33	0.0	0.0	0.0	7.022541582908527	0.0
34	0.0	0.0	0.0	7.262062097637003	0.0
35	0.0	0.0	0.0	7.526756329523957	0.0
36	0.0	0.0	0.0	7.783121022645239	0.0
37	0.0	0.0	0.0	8.037634707151927	0.0
38	0.0	0.0	0.0	8.294184501134668	0.0
39	0.0	0.0	0.0	8.576648415721726	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGT	25	3.8880513E-5	45.0	45
CGAACCG	20	7.0299633E-4	45.0	30
CAACGCT	20	7.0299633E-4	45.0	30
CGCATAG	45	3.8380676E-10	45.0	2
CCAATCG	20	7.0299633E-4	45.0	21
TGACGTT	20	7.0299633E-4	45.0	28
TAGCACG	25	3.8880513E-5	45.0	1
TATAGCG	40	6.8030204E-9	45.0	1
ACCGTGT	20	7.0299633E-4	45.0	37
CGATCTA	25	3.8880513E-5	45.0	10
CACGACC	60	0.0	44.999996	27
AGGCGAT	205	0.0	42.804874	7
CGAATAT	175	0.0	42.42857	14
TATCGCG	70	0.0	41.785713	32
AATAGGC	65	0.0	41.53846	4
TACGAAT	185	0.0	41.351353	12
CGTTTTA	420	0.0	41.25	1
GCGATAC	55	6.002665E-11	40.909092	9
ATGGGCG	50	1.0786607E-9	40.5	5
TACGGGT	50	1.0786607E-9	40.5	4
>>END_MODULE