##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934615.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	290259
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.025242972655455	33.0	31.0	34.0	30.0	34.0
2	32.225998160263764	33.0	31.0	34.0	30.0	34.0
3	32.110897508776645	33.0	31.0	34.0	30.0	34.0
4	35.87036749937125	37.0	35.0	37.0	35.0	37.0
5	32.188135423880055	37.0	35.0	37.0	10.0	37.0
6	34.03329440258528	37.0	35.0	37.0	19.0	37.0
7	20.609686521348177	32.0	0.0	37.0	0.0	37.0
8	28.027640831119793	35.0	17.0	37.0	17.0	37.0
9	34.87766787593149	37.0	32.0	39.0	32.0	39.0
10	36.32815175412304	37.0	35.0	39.0	32.0	39.0
11	36.75966636693436	39.0	37.0	39.0	33.0	39.0
12	36.977967952759435	39.0	37.0	39.0	33.0	39.0
13	36.638271336978356	39.0	35.0	39.0	33.0	39.0
14	37.99644110949187	40.0	37.0	41.0	33.0	41.0
15	38.23593066881647	40.0	37.0	41.0	33.0	41.0
16	38.33434622182258	40.0	37.0	41.0	34.0	41.0
17	38.31743374021133	40.0	37.0	41.0	34.0	41.0
18	38.23218918276436	40.0	37.0	41.0	34.0	41.0
19	38.17625982312349	40.0	37.0	41.0	34.0	41.0
20	38.174602682431896	40.0	37.0	41.0	34.0	41.0
21	38.04254820694621	40.0	36.0	41.0	34.0	41.0
22	38.00743129412008	40.0	36.0	41.0	34.0	41.0
23	37.95854047591978	40.0	36.0	41.0	34.0	41.0
24	37.8391092093613	40.0	36.0	41.0	33.0	41.0
25	37.69898263275213	40.0	35.0	41.0	33.0	41.0
26	37.55001223045625	39.0	35.0	41.0	33.0	41.0
27	37.49936091559607	39.0	35.0	41.0	33.0	41.0
28	37.416204148708566	39.0	35.0	41.0	33.0	41.0
29	37.419542546484344	39.0	35.0	41.0	33.0	41.0
30	37.281717362769115	39.0	35.0	41.0	33.0	41.0
31	37.09540789432886	39.0	35.0	41.0	32.0	41.0
32	37.00464412817518	39.0	35.0	41.0	32.0	41.0
33	36.86866212589446	39.0	35.0	41.0	31.0	41.0
34	36.723257504504595	39.0	35.0	41.0	31.0	41.0
35	36.60176945417713	39.0	35.0	41.0	31.0	41.0
36	36.444995676275326	39.0	35.0	41.0	30.0	41.0
37	36.354331820891	39.0	35.0	41.0	30.0	41.0
38	36.24401655073572	39.0	35.0	40.0	30.0	41.0
39	36.130545478348644	39.0	35.0	40.0	30.0	41.0
40	36.025611608942356	39.0	35.0	40.0	29.0	41.0
41	35.904640338456346	39.0	35.0	40.0	29.0	41.0
42	35.81831054334233	38.0	35.0	40.0	29.0	41.0
43	35.74033535566511	38.0	35.0	40.0	28.0	41.0
44	35.531132540248535	38.0	35.0	40.0	27.0	41.0
45	35.42347007327938	38.0	34.0	40.0	27.0	41.0
46	35.268343100472336	38.0	34.0	40.0	26.0	41.0
47	35.22865785384777	38.0	34.0	40.0	26.0	41.0
48	35.13077286147889	38.0	34.0	40.0	26.0	41.0
49	35.01930000447876	38.0	34.0	40.0	26.0	41.0
50	34.85399246879511	37.0	34.0	40.0	26.0	41.0
51	32.970343727498545	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	10.0
10	13.0
11	7.0
12	5.0
13	4.0
14	7.0
15	16.0
16	31.0
17	55.0
18	111.0
19	186.0
20	321.0
21	565.0
22	901.0
23	1354.0
24	1924.0
25	2612.0
26	3116.0
27	3603.0
28	3963.0
29	4837.0
30	6000.0
31	7774.0
32	10212.0
33	15624.0
34	22149.0
35	28083.0
36	32801.0
37	54675.0
38	55279.0
39	34007.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.750767418064555	21.159378348302724	26.655159702197	14.434694531435719
2	31.74130690176704	26.18592360615864	26.895290068525007	15.177479423549311
3	32.41243165586597	23.546556695916408	27.692509103938207	16.34850254427942
4	28.329870908395606	26.075332720087925	27.33317485418196	18.261621517334518
5	23.230976472736415	36.76165080152553	23.24751342766288	16.759859298075167
6	27.519560116998953	34.36896013560303	24.16979318470745	13.941686562690563
7	49.79277128357777	43.33302326542846	4.493228461477508	2.3809769895162596
8	86.74907582538354	4.124936694469422	6.8462993395553635	2.2796881405916785
9	81.19851580829534	7.19667607205978	8.023868338277195	3.580939781367675
10	41.92600401710197	33.353315487202806	13.389076652231283	11.33160384346394
11	36.55356078536755	23.156215655673037	22.804116323697112	17.4861072352623
12	32.80105009663783	22.08096906555869	26.168353091549275	18.949627746254208
13	24.837128219969063	28.152443162830437	27.859256732780036	19.151171884420464
14	20.391788023799435	29.326222442714954	29.322088203983338	20.959901329502273
15	19.38131117381373	24.40923451124685	35.83075804712343	20.378696267815986
16	22.935722923320206	22.634612535700875	34.114359933714375	20.315304607264547
17	23.228220313582007	22.28044608435914	28.83907131217292	25.65226228988593
18	24.11673712098505	23.560337491688458	30.12998735611988	22.192938031206612
19	25.826933876296685	25.447272952776657	27.28184138993106	21.443951780995594
20	28.082850144181577	24.158079508301206	27.62946196328107	20.129608384236146
21	25.37147857603037	25.124802331710644	29.171533010173672	20.33218608208531
22	25.60402950468375	23.148291698104106	27.808956828212043	23.438721969000103
23	22.93916812226322	25.367344337298757	27.880272446332416	23.813215094105608
24	22.8278881964039	23.77635146541537	31.8894504563166	21.506309881864127
25	22.81720807968056	25.179580994904548	29.770653106363625	22.23255781905126
26	21.12389279918969	28.525558208358742	27.498888923340882	22.85166006911069
27	21.75643132512687	27.56538126294103	29.142248819158063	21.53593859277404
28	20.027975015417265	27.170905983966044	32.31010924725848	20.49100975335821
29	20.95473353108775	24.96287798138904	31.063636269676394	23.01875221784682
30	22.411019124299333	26.036746491926177	29.825431769557532	21.726802614216957
31	25.2202343424321	26.42364233322653	26.72888695957748	21.62723636476388
32	26.368519150138326	26.469807999062905	26.578676285662116	20.582996565136654
33	23.801501417699363	27.181241580795085	27.086498609862225	21.930758391643327
34	21.87563520855512	27.816191745992374	28.356398940256806	21.951774105195703
35	22.81720807968056	26.523208582679608	28.29197372002246	22.367609617617372
36	24.801298150961728	27.34867824942551	26.669974057651956	21.1800495419608
37	22.9877454273597	28.11936925297751	28.294040839388266	20.598844480274515
38	22.368298657405973	28.233060818096938	26.65205902314829	22.746581501348796
39	22.579489352612665	27.36245904519756	28.289217560868053	21.768834041321718
40	24.19356505741424	25.523756369311545	27.732817931571457	22.549860641702754
41	21.20347689477329	25.87757830075898	29.014431938372283	23.904512866095455
42	23.027020695310053	25.22988089947254	27.285286588874076	24.457811816343337
43	22.60429478500236	25.18268167395326	27.37348368181521	24.83953985922917
44	22.04307187718555	27.532651872982406	27.534029952559614	22.890246297272434
45	22.378978774129312	27.976393496842476	26.73129859883759	22.913329130190625
46	22.347627463747894	28.76568857468674	26.77160742647084	22.11507653509452
47	22.106808057631287	27.708357019076068	28.318501751883662	21.866333171408982
48	22.376222614974903	25.352874501738103	29.708984045283692	22.5619188380033
49	22.28802552203377	25.694293716990686	29.064731842940272	22.952948918035272
50	21.802252471068943	27.943664106883855	28.080093985027165	22.17398943702004
51	20.985051281786266	27.59535449374524	27.20122373466456	24.218370489803934
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	386.0
1	653.0
2	920.0
3	1658.5
4	2397.0
5	1716.5
6	1036.0
7	984.5
8	933.0
9	968.5
10	1004.0
11	957.5
12	911.0
13	969.5
14	1028.0
15	1036.0
16	1044.0
17	1033.0
18	1022.0
19	1128.5
20	1235.0
21	1288.5
22	1342.0
23	1477.5
24	1613.0
25	2009.0
26	2885.0
27	3365.0
28	3693.5
29	4022.0
30	4555.5
31	5089.0
32	6074.0
33	7059.0
34	7895.0
35	8731.0
36	9174.0
37	9617.0
38	10606.0
39	11595.0
40	13061.0
41	14527.0
42	16474.5
43	18422.0
44	19617.0
45	20812.0
46	21003.5
47	21195.0
48	20968.0
49	20741.0
50	20111.5
51	19482.0
52	18631.5
53	17781.0
54	17128.5
55	16476.0
56	15917.0
57	15358.0
58	14428.5
59	13499.0
60	12745.0
61	11991.0
62	10972.5
63	9954.0
64	8663.0
65	7372.0
66	6301.5
67	5231.0
68	4514.0
69	3797.0
70	3283.0
71	2769.0
72	2263.5
73	1758.0
74	1372.0
75	764.0
76	542.0
77	436.5
78	331.0
79	283.0
80	235.0
81	193.5
82	152.0
83	96.0
84	40.0
85	28.0
86	16.0
87	23.0
88	30.0
89	18.0
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	290259.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.81345281283268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.36907311746671	22.106119017842683
2	7.831464449204149	5.139547783186741
3	4.288984082986855	4.222091304662388
4	3.0406114820881105	3.9909184555862174
5	2.3476544454243835	3.851732418288494
6	1.8415858216790557	3.6257273676268436
7	1.7155936331947417	3.940618551018229
8	1.4741086052664734	3.8696474527921616
9	1.396413422367813	4.123903134786518
>10	8.65041367435219	43.640679531039524
>50	0.03464785183318635	0.7441629716908003
>100	0.008399479232287599	0.5216031199721628
>500	0.0010499349040359498	0.2232488915072401
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	648	0.2232488915072401	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	402	0.13849699750912114	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02687255175550112	0.0
2	0.0	0.0	0.0	0.12264908237126153	0.0
3	0.0	0.0	0.0	0.16502502937032099	0.0
4	0.0	0.0	0.0	0.20567837689787397	0.0
5	0.0	0.0	0.0	0.33349525768365496	0.0
6	0.0	0.0	0.0	0.4327169872424283	0.0
7	0.0	0.0	0.0	0.5222921597607654	0.0
8	0.0	0.0	0.0	0.7393396931705821	0.0
9	0.0	0.0	0.0	0.8151340699168673	0.0
10	0.0	0.0	0.0	1.0184008075546322	0.0
11	0.0	0.0	0.0	1.3157214763366512	0.0
12	0.0	0.0	0.0	1.5975387498751115	0.0
13	0.0	0.0	0.0	1.7070960762629237	0.0
14	0.0	0.0	0.0	1.7539507818879	0.0
15	0.0	0.0	0.0	1.8511053920808658	0.0
16	0.0	0.0	0.0	2.089857678831664	0.0
17	0.0	0.0	0.0	2.3423907613545145	0.0
18	0.0	0.0	0.0	2.653492225908585	0.0
19	0.0	0.0	0.0	2.8216179343276178	0.0
20	0.0	0.0	0.0	3.0111038761933306	0.0
21	0.0	0.0	0.0	3.2305630488632566	0.0
22	0.0	0.0	0.0	3.4603578183622212	0.0
23	0.0	0.0	0.0	3.7104792616249624	0.0
24	0.0	0.0	0.0	3.895486444864759	0.0
25	0.0	0.0	0.0	4.065679272649599	0.0
26	0.0	0.0	0.0	4.226914583182606	0.0
27	0.0	0.0	0.0	4.36885677963474	0.0
28	0.0	0.0	0.0	4.529058530484843	0.0
29	0.0	0.0	0.0	4.713721193830338	0.0
30	0.0	0.0	0.0	4.922844769671225	0.0
31	0.0	0.0	0.0	5.129901226146304	0.0
32	0.0	0.0	0.0	5.3090515711829775	0.0
33	0.0	0.0	0.0	5.500604632414499	0.0
34	0.0	0.0	0.0	5.690435094174513	0.0
35	0.0	0.0	0.0	5.906793587795727	0.0
36	0.0	0.0	0.0	6.099724728604453	0.0
37	0.0	0.0	0.0	6.296445588250494	0.0
38	0.0	0.0	0.0	6.523139678700747	0.0
39	0.0	0.0	0.0	6.752934448199711	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	30	2.1598753E-6	45.000004	1
GTACATC	25	3.8835773E-5	45.000004	36
GACCGTC	25	3.8835773E-5	45.000004	26
GATCAAT	25	3.8835773E-5	45.000004	9
ACGGGTG	20	7.0245634E-4	45.000004	5
CCCCGTG	20	7.0245634E-4	45.000004	13
CCGTAGG	30	2.1598753E-6	45.000004	3
ATATAGC	25	3.8835773E-5	45.000004	12
TACGAAT	45	3.8198777E-10	45.000004	12
AGACCTA	40	6.7848305E-9	45.000004	32
AGACCGT	25	3.8835773E-5	45.000004	25
TCGTCCC	30	2.1598753E-6	45.000004	38
AAATTCG	20	7.0245634E-4	45.000004	30
CGAATAT	45	3.8198777E-10	45.000004	14
AATTCGC	20	7.0245634E-4	45.000004	12
CGTACAT	25	3.8835773E-5	45.000004	35
GCTACGA	45	3.8198777E-10	45.000004	10
CACCGTT	20	7.0245634E-4	45.000004	31
AGGATAC	20	7.0245634E-4	45.000004	7
TATACGA	20	7.0245634E-4	45.000004	33
>>END_MODULE