##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934614.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556576
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6554163312827	31.0	31.0	34.0	30.0	34.0
2	31.826065083654342	31.0	31.0	34.0	30.0	34.0
3	30.896427442074398	31.0	31.0	34.0	26.0	34.0
4	35.11990096590583	37.0	35.0	37.0	32.0	37.0
5	31.59231803024205	35.0	35.0	37.0	0.0	37.0
6	33.68814142183637	37.0	35.0	37.0	17.0	37.0
7	21.47971705571207	32.0	0.0	37.0	0.0	37.0
8	28.55014948542517	35.0	17.0	37.0	17.0	37.0
9	35.112990858391306	37.0	32.0	39.0	32.0	39.0
10	36.39193569251998	37.0	35.0	39.0	32.0	39.0
11	36.74669407232795	39.0	37.0	39.0	33.0	39.0
12	37.175384853101825	39.0	37.0	39.0	34.0	39.0
13	37.06649945380325	39.0	37.0	39.0	34.0	39.0
14	38.53763008106709	40.0	38.0	41.0	34.0	41.0
15	38.68570150347841	40.0	38.0	41.0	35.0	41.0
16	38.70851420111539	40.0	38.0	41.0	35.0	41.0
17	38.67260356177773	40.0	38.0	41.0	35.0	41.0
18	38.45500165296384	40.0	38.0	41.0	34.0	41.0
19	38.251455326855634	40.0	37.0	41.0	34.0	41.0
20	38.03148536767665	40.0	36.0	41.0	34.0	41.0
21	37.922612545276834	40.0	36.0	41.0	33.0	41.0
22	37.794428793192665	39.0	35.0	41.0	33.0	41.0
23	37.6414254297706	39.0	35.0	41.0	33.0	41.0
24	37.43304059104237	39.0	35.0	41.0	33.0	41.0
25	37.277149931006726	39.0	35.0	41.0	33.0	41.0
26	37.18098336974645	39.0	35.0	41.0	33.0	41.0
27	37.1083104553556	39.0	35.0	41.0	33.0	41.0
28	37.038424222388315	39.0	35.0	41.0	33.0	41.0
29	36.998091904789284	39.0	35.0	41.0	33.0	41.0
30	36.815847251767956	39.0	35.0	41.0	32.0	41.0
31	36.57225428333238	39.0	35.0	41.0	31.0	41.0
32	36.26018369458978	39.0	35.0	40.0	30.0	41.0
33	35.76497369631461	39.0	35.0	40.0	26.0	41.0
34	35.236551701834074	39.0	34.0	40.0	23.0	41.0
35	34.712872994883	39.0	34.0	40.0	20.0	41.0
36	34.31896991605819	38.0	34.0	40.0	16.0	41.0
37	34.127039254297706	38.0	34.0	40.0	15.0	41.0
38	33.95507352095671	38.0	33.0	40.0	15.0	41.0
39	33.85734562755131	38.0	33.0	40.0	13.0	41.0
40	33.78699584603001	38.0	33.0	40.0	12.0	41.0
41	33.67270956706721	38.0	33.0	40.0	10.0	41.0
42	33.50876609843041	38.0	33.0	40.0	10.0	41.0
43	33.39665562295176	38.0	33.0	40.0	10.0	41.0
44	33.26618826539412	38.0	33.0	40.0	10.0	41.0
45	33.185025944345426	38.0	33.0	40.0	10.0	41.0
46	33.05183478985799	38.0	33.0	40.0	10.0	41.0
47	33.01578760133387	38.0	33.0	40.0	10.0	41.0
48	32.91607794802507	38.0	32.0	40.0	10.0	41.0
49	32.81083984936469	38.0	32.0	40.0	10.0	41.0
50	32.64866253665268	38.0	31.0	40.0	10.0	41.0
51	31.1797328666705	35.0	28.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	11.0
10	22.0
11	15.0
12	17.0
13	9.0
14	15.0
15	20.0
16	44.0
17	86.0
18	206.0
19	442.0
20	941.0
21	1795.0
22	3475.0
23	5973.0
24	9956.0
25	14642.0
26	16880.0
27	15832.0
28	13832.0
29	12487.0
30	12537.0
31	14204.0
32	17322.0
33	23665.0
34	32274.0
35	43200.0
36	54266.0
37	92972.0
38	100826.0
39	68595.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.312431725406775	18.697536365204392	23.026504915770712	24.96352699361812
2	41.773091186109355	20.94466883228885	22.859950842292875	14.422289139308916
3	27.489507273040882	20.743438452250906	38.03954895647674	13.727505318231472
4	22.878636520439258	22.29956016788363	39.45822313574426	15.363580175932848
5	18.910984304030357	34.20305582705686	33.37729258897257	13.508667279940207
6	21.9421965733341	30.10711924337377	36.12193123670442	11.828752946587707
7	43.41958690277698	40.33249726901627	14.32688437877307	1.9210314494336804
8	75.55949232449835	3.037321048697752	19.648529580865866	1.7546570459380213
9	70.57724371873742	6.30839274420744	20.672468809291093	2.4418947277640433
10	31.98467055712068	32.14242080147186	27.227189099062844	8.64571954234462
11	21.090560857816364	23.68373771057322	41.240369688955326	13.985331742655092
12	20.205326855631576	22.337111194158567	41.89059535445294	15.566966595756915
13	18.396768815040534	24.045054044730637	42.7451057896855	14.813071350543321
14	15.944273558328062	26.78609929281895	41.128614960041396	16.14101218881159
15	15.733340999252574	25.78767320186282	43.485885126200195	14.993100672684415
16	19.23564796182372	24.966761053297304	41.62594147070661	14.171649514172369
17	19.531744092450985	24.654853964238487	39.78648019318117	16.026921750129365
18	19.485568907031563	24.262814063128847	40.882467084459265	15.369149945380325
19	18.94835566032312	26.282304662795376	39.297957511642615	15.47138216523889
20	19.115808083711837	26.16659719427356	39.98753090323694	14.73006381877767
21	19.029027482320473	25.509903409417582	40.99458115333755	14.466487954924395
22	19.23205456218019	23.462024952567127	40.629312079572244	16.676608405680447
23	17.107996032886792	25.27327804289082	40.94535157822112	16.673374346001264
24	16.85699706778589	25.359339964353477	41.8345383200138	15.949124647846835
25	16.59270252400391	26.872879894210318	40.2604496061634	16.273967975622377
26	17.29772753406543	27.45968205599954	39.123497958949	16.119092450986027
27	16.409439142183636	27.29222963261082	40.49941068245846	15.798920542747084
28	15.689681193583628	26.394059391709305	41.67301500603691	16.243244408670154
29	16.081900764675446	25.402820100040245	41.529638360259874	16.985640775024436
30	17.412356982694188	25.953149255447595	40.5191743804979	16.11531938136032
31	17.623109871787502	26.68548410279998	39.09582878169379	16.595577243718736
32	17.67162076697522	26.87665296383603	38.81212987983672	16.639596389352036
33	17.438768470074166	27.292768642557352	38.687618582188236	16.580844305180246
34	16.960666647501867	27.70241620192031	38.23395187719198	17.102965273385845
35	17.31479618237222	27.203113321451163	37.67643592249756	17.805654573679067
36	18.179547806588857	28.66922037601334	36.07988846087507	17.07134335652274
37	17.464281607543263	29.083539354912897	36.5569122635543	16.895266773989537
38	17.593464324728338	29.57835048582763	35.36911401138389	17.45907117806014
39	18.281600356465244	28.7374949692405	34.93790605415972	18.042998620134536
40	19.54144627148853	27.91837952049675	34.52700080492152	18.013173403093198
41	18.037428850687057	27.77410452480883	34.64522365319381	19.5432429713103
42	18.786652676364053	28.47966854481688	33.90731903639395	18.826359742425115
43	18.961291899039846	29.084617374805955	33.52731702409015	18.426773702064047
44	17.933938940953258	29.745443569252	33.43550566319784	18.885111826596905
45	17.96178778819065	29.707353533030528	33.37531621916863	18.95554245961019
46	18.376286437072388	29.934456390501925	32.77396078882309	18.9152963836026
47	17.592386304835276	29.19565342379118	34.07854452940838	19.13341574196516
48	18.23452682113494	28.383904444316677	34.34068303340425	19.040885701144138
49	18.571228367734147	28.091401713332946	33.78694014833554	19.550429770597365
50	17.434097050537574	28.812956361754726	34.4738184901972	19.279128097510494
51	17.72390473178865	28.322097970447878	33.57474989938481	20.37924739837866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	710.0
1	966.5
2	1223.0
3	15348.0
4	29473.0
5	20977.5
6	12482.0
7	10802.0
8	9122.0
9	8768.5
10	8415.0
11	8322.5
12	8230.0
13	7980.0
14	7730.0
15	7296.0
16	6862.0
17	6503.5
18	6145.0
19	5903.5
20	5662.0
21	5554.5
22	5447.0
23	5745.0
24	6043.0
25	6684.5
26	8658.0
27	9990.0
28	11407.5
29	12825.0
30	14329.5
31	15834.0
32	17944.0
33	20054.0
34	22076.5
35	24099.0
36	25100.0
37	26101.0
38	27424.5
39	28748.0
40	30774.0
41	32800.0
42	35073.5
43	37347.0
44	38266.0
45	39185.0
46	39371.0
47	39557.0
48	37800.0
49	36043.0
50	33766.5
51	31490.0
52	28445.5
53	25401.0
54	21923.5
55	18446.0
56	16232.0
57	14018.0
58	12043.0
59	10068.0
60	8688.5
61	7309.0
62	6206.0
63	5103.0
64	4070.5
65	3038.0
66	2417.0
67	1796.0
68	1413.0
69	1030.0
70	782.0
71	534.0
72	469.0
73	404.0
74	321.0
75	173.0
76	108.0
77	73.0
78	38.0
79	38.0
80	38.0
81	38.0
82	38.0
83	25.0
84	12.0
85	8.0
86	4.0
87	4.0
88	4.0
89	4.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	556576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.418359178193924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.73728972386658	35.761185053960894
2	7.545739452008965	6.854302093928226
3	3.063467544789174	4.174130078399237
4	1.849100550729476	3.3593245187851015
5	1.2937425399835931	2.937983168254187
6	1.0571614154408593	2.88087221254925
7	0.849069929907181	2.6994354130749794
8	0.6855773773837825	2.491023965636867
9	0.624696065345634	2.5535403245765256
>10	4.248269714307104	30.265886662274106
>50	0.03233848363484664	0.9882378846961338
>100	0.010925163389406014	0.7814204763693023
>500	8.740130711524811E-4	0.21634450577661662
>1k	8.740130711524811E-4	0.710959651093432
>5k	4.3700653557624057E-4	1.1928719997407946
>10k+	4.3700653557624057E-4	2.1324819908843753
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10744	1.9303742885068704	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6010	1.079816592882194	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2278	0.40928821939860865	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1304	0.23428965675846603	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	572	0.10277122980509401	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7966998217673776E-4	0.0	0.0	0.025513137469096763	0.0
2	1.7966998217673776E-4	0.0	0.0	0.10151353992985684	0.0
3	1.7966998217673776E-4	0.0	0.0	0.16152331397688727	0.0
4	1.7966998217673776E-4	0.0	0.0	0.21758034841602944	0.0
5	1.7966998217673776E-4	0.0	0.0	0.36329270396136376	0.0
6	1.7966998217673776E-4	0.0	0.0	0.550868165353878	0.0
7	1.7966998217673776E-4	0.0	0.0	0.7332331972632669	0.0
8	1.7966998217673776E-4	0.0	0.0	1.136053297303513	0.0
9	1.7966998217673776E-4	0.0	0.0	1.3419550968780545	0.0
10	1.7966998217673776E-4	0.0	0.0	1.671110504225838	0.0
11	1.7966998217673776E-4	0.0	0.0	2.049136146725694	0.0
12	1.7966998217673776E-4	0.0	0.0	2.3256482492956936	0.0
13	1.7966998217673776E-4	0.0	0.0	2.465072155464842	0.0
14	1.7966998217673776E-4	0.0	0.0	2.5274176392801704	0.0
15	1.7966998217673776E-4	0.0	0.0	2.623002069798195	0.0
16	1.7966998217673776E-4	0.0	0.0	2.8145302707985973	0.0
17	1.7966998217673776E-4	0.0	0.0	3.0836759040993504	0.0
18	1.7966998217673776E-4	0.0	0.0	3.367554475938596	0.0
19	1.7966998217673776E-4	0.0	0.0	3.5624964066003564	0.0
20	1.7966998217673776E-4	0.0	0.0	3.7455801184384523	0.0
21	1.7966998217673776E-4	0.0	0.0	3.976096705571207	0.0
22	1.7966998217673776E-4	0.0	0.0	4.225658310814696	0.0
23	1.7966998217673776E-4	0.0	0.0	4.528941240729029	0.0
24	1.7966998217673776E-4	0.0	0.0	4.747240269073766	0.0
25	1.7966998217673776E-4	0.0	0.0	4.9549387684700745	0.0
26	1.7966998217673776E-4	0.0	0.0	5.1437719197378255	0.0
27	3.593399643534755E-4	0.0	0.0	5.337276490542172	0.0
28	3.593399643534755E-4	0.0	0.0	5.552700799172081	0.0
29	3.593399643534755E-4	0.0	0.0	5.7871701259127235	0.0
30	3.593399643534755E-4	0.0	0.0	6.0636822284827225	0.0
31	3.593399643534755E-4	0.0	0.0	6.338397631230955	0.0
32	3.593399643534755E-4	0.0	0.0	6.589216926349681	0.0
33	3.593399643534755E-4	0.0	0.0	6.851535100327718	0.0
34	3.593399643534755E-4	0.0	0.0	7.101635715517737	0.0
35	3.593399643534755E-4	0.0	0.0	7.376171448283793	0.0
36	3.593399643534755E-4	0.0	0.0	7.647113781406313	0.0
37	3.593399643534755E-4	0.0	0.0	7.9265006036911405	0.0
38	3.593399643534755E-4	0.0	0.0	8.218284654746162	0.0
39	3.593399643534755E-4	0.0	0.0	8.506654976139826	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	30	2.163475E-6	45.000004	2
TCGCAAG	20	7.0301467E-4	45.0	2
CGAACGG	20	7.0301467E-4	45.0	2
TTGTGCG	25	3.8882034E-5	45.0	1
CACGACC	40	6.8048394E-9	45.0	27
AAGCGCG	20	7.0301467E-4	45.0	1
AAGCGAT	20	7.0301467E-4	45.0	6
CGTTCTA	20	7.0301467E-4	45.0	1
ATCGTAG	20	7.0301467E-4	45.0	1
GCTTACG	20	7.0301467E-4	45.0	1
ACTACGG	20	7.0301467E-4	45.0	2
GCCTACG	20	7.0301467E-4	45.0	1
TATCGAC	25	3.8882034E-5	45.0	30
AACACGT	40	6.8048394E-9	45.0	41
CGTTATT	1100	0.0	44.590908	1
CGTTTTA	2465	0.0	44.361053	1
CGTTTTT	5030	0.0	43.747513	1
CGGTTTT	625	0.0	42.84	1
CGGTTTA	235	0.0	41.17021	1
TAGGCGA	55	6.002665E-11	40.909092	6
>>END_MODULE