##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934613.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446485
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0507654232505	33.0	31.0	34.0	30.0	34.0
2	32.24676304915059	33.0	31.0	34.0	30.0	34.0
3	32.1585338813174	33.0	31.0	34.0	30.0	34.0
4	35.89166937299125	37.0	35.0	37.0	35.0	37.0
5	32.20610546826881	37.0	35.0	37.0	10.0	37.0
6	34.03412432668511	37.0	35.0	37.0	19.0	37.0
7	20.626294276403463	32.0	0.0	37.0	0.0	37.0
8	28.017577298229504	35.0	17.0	37.0	17.0	37.0
9	34.83289248239023	37.0	32.0	39.0	32.0	39.0
10	36.29086979405803	37.0	35.0	39.0	32.0	39.0
11	36.744793218137225	38.0	37.0	39.0	33.0	39.0
12	37.05105658644747	39.0	37.0	39.0	34.0	39.0
13	36.801713383428336	39.0	37.0	39.0	33.0	39.0
14	38.17681669037034	40.0	37.0	41.0	33.0	41.0
15	38.38952708377661	40.0	38.0	41.0	34.0	41.0
16	38.459253950300685	40.0	38.0	41.0	34.0	41.0
17	38.426907958834	40.0	38.0	41.0	34.0	41.0
18	38.339258877677864	40.0	37.0	41.0	34.0	41.0
19	38.28919672553389	40.0	37.0	41.0	34.0	41.0
20	38.27295205885976	40.0	37.0	41.0	34.0	41.0
21	38.19319574005846	40.0	37.0	41.0	34.0	41.0
22	38.16569649596291	40.0	37.0	41.0	34.0	41.0
23	38.096542996965184	40.0	37.0	41.0	34.0	41.0
24	37.987058915753046	40.0	36.0	41.0	34.0	41.0
25	37.86365051457496	40.0	36.0	41.0	33.0	41.0
26	37.719361232740184	40.0	36.0	41.0	33.0	41.0
27	37.67447282663471	40.0	36.0	41.0	33.0	41.0
28	37.59285082365589	40.0	36.0	41.0	33.0	41.0
29	37.56515672419006	40.0	36.0	41.0	33.0	41.0
30	37.460696327984145	40.0	36.0	41.0	33.0	41.0
31	37.35948800071671	40.0	36.0	41.0	33.0	41.0
32	37.25707918519099	40.0	35.0	41.0	32.0	41.0
33	37.12427517161831	40.0	35.0	41.0	32.0	41.0
34	36.9844742824507	40.0	35.0	41.0	31.0	41.0
35	36.87999148907578	40.0	35.0	41.0	31.0	41.0
36	36.74250646718255	39.0	35.0	41.0	31.0	41.0
37	36.669200533052624	39.0	35.0	41.0	30.0	41.0
38	36.58283704939696	39.0	35.0	41.0	30.0	41.0
39	36.51563210410204	39.0	35.0	41.0	30.0	41.0
40	36.40539099857778	39.0	35.0	41.0	30.0	41.0
41	36.279989249358884	39.0	35.0	40.0	30.0	41.0
42	36.17315475323919	39.0	35.0	40.0	29.0	41.0
43	36.0860297658376	39.0	35.0	40.0	29.0	41.0
44	35.94540018141707	39.0	35.0	40.0	29.0	41.0
45	35.88922808157049	38.0	35.0	40.0	29.0	41.0
46	35.75734235192672	38.0	35.0	40.0	28.0	41.0
47	35.71728053574028	38.0	35.0	40.0	28.0	41.0
48	35.59329652731895	38.0	35.0	40.0	28.0	41.0
49	35.48310693528338	38.0	34.0	40.0	27.0	41.0
50	35.32934813039632	38.0	34.0	40.0	27.0	41.0
51	33.53148258060181	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	9.0
9	19.0
10	36.0
11	24.0
12	11.0
13	16.0
14	21.0
15	31.0
16	58.0
17	107.0
18	164.0
19	260.0
20	493.0
21	755.0
22	1204.0
23	1753.0
24	2492.0
25	3501.0
26	4372.0
27	5097.0
28	5865.0
29	6778.0
30	8436.0
31	11030.0
32	14436.0
33	22011.0
34	31208.0
35	40109.0
36	50714.0
37	86380.0
38	90999.0
39	58084.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.0829031210455	21.147854911139234	24.55894374951006	13.210298218305205
2	31.183130452310827	25.18628845313952	26.954992888898843	16.675588205650808
3	32.250355555057844	24.422097046933267	26.880634287826016	16.446913110182873
4	29.230769230769234	25.51507889402779	26.67749196501562	18.576659910187352
5	23.84469803016899	37.7647625340157	22.218887532615877	16.171651903199436
6	28.233423295295474	34.93017682564924	23.495078222112724	13.341321656942561
7	49.66370650749745	43.507396665061535	4.318622126163254	2.5102747012777584
8	86.05619449701558	4.571038220768895	6.794405187184339	2.578362095031188
9	79.52674781907567	8.197363853208953	8.000044794337995	4.275843533377381
10	41.43543456107148	32.88553926783654	14.205628408569156	11.473397762522817
11	34.03899347122524	26.24612249011725	23.059453285104762	16.65543075355275
12	32.41519871888193	22.357973952092454	26.854429600098545	18.372397728927066
13	25.968173622854074	25.41070808649787	27.651544844731625	20.96957344591644
14	20.199334804080767	27.633403137843377	29.50804618296247	22.659215875113386
15	20.42196266391928	26.795301073944255	32.81095669507374	19.971779567062722
16	23.653426206927445	24.556704032610277	32.03892627971825	19.750943480744034
17	23.452299629326852	24.840476163812895	28.2435020213445	23.46372218551575
18	23.69262125267366	25.8973985688209	28.546087774505303	21.863892404000136
19	26.61858741055131	26.15429409722611	26.518920008510925	20.70819848371166
20	27.847520073462718	25.842973448156155	27.038310357570804	19.27119612081033
21	26.18766587903289	25.608251117058806	28.13711546860477	20.066967535303537
22	26.49719475458302	23.393619046552516	28.012587208976786	22.096598989887678
23	24.509893949404795	26.54579661130833	28.103295743418034	20.841013695868842
24	23.341209671097573	25.02010145917556	30.193847497676295	21.444841372050572
25	23.249605249896412	26.560354771156923	28.50734067213904	21.68269930680762
26	21.898608015946785	28.79156074672161	27.584801281118065	21.725029956213536
27	22.10309416889705	28.753261587735313	28.586626650391388	20.557017592976248
28	21.925036675364236	27.76621835000056	30.13449499983202	20.174249974803185
29	21.930635967613693	25.911508785289538	30.104258821684937	22.05359642541183
30	22.31205975564689	26.234475962238374	28.85427281991556	22.59919146219918
31	23.999014524564092	26.789253838314835	27.208976785334333	22.002754851786737
32	24.84338779578261	27.619516893064716	26.51264880119153	21.02444650996114
33	24.361624690639104	27.941812154943612	26.587007402264355	21.10955575215293
34	22.7761290972821	28.455155268374078	28.017290614466333	20.751425019877487
35	23.252068938486175	26.807171573513106	27.704402163566527	22.23635732443419
36	24.145044066430003	28.72011377761851	26.559010941017053	20.575831214934432
37	23.471785166354973	29.181943402353944	27.543142546782086	19.803128884508997
38	23.1129825190096	27.760395086061123	27.156119466499433	21.97050292842985
39	23.326203567869022	27.06809859233793	27.73441437002363	21.87128346976942
40	23.81938922920143	26.687346719374673	27.836545460653774	21.656718590770126
41	20.976292596615785	27.0331590087013	28.919000638319314	23.071547756363593
42	22.283167407639674	27.522313179614095	27.167094079308374	23.027425333437854
43	23.205034883590713	27.1471605989003	26.75319439622832	22.89461012128067
44	21.739140172682173	28.107327233837644	27.588384828157718	22.565147765322465
45	22.396721054458716	27.265865594588846	26.808739375342956	23.528673975609482
46	21.962439947590624	28.812838057269563	26.742219783419372	22.48250221172044
47	22.170733619270525	28.48270378624142	27.782792255058958	21.563770339429098
48	22.838169255406115	26.24612249011725	28.588418423911214	22.327289830565416
49	21.82402544318398	26.93595529525068	28.24596570993426	22.994053551631076
50	20.912460664971945	27.372476119018557	28.62134226233804	23.093720953671458
51	20.86654646852638	27.67103038175975	27.053764404179308	24.408658745534563
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	325.0
1	910.0
2	1495.0
3	2453.0
4	3411.0
5	2545.5
6	1680.0
7	1497.0
8	1314.0
9	1297.5
10	1281.0
11	1315.0
12	1349.0
13	1300.5
14	1252.0
15	1241.5
16	1231.0
17	1300.0
18	1369.0
19	1591.0
20	1813.0
21	1821.0
22	1829.0
23	2185.5
24	2542.0
25	3173.0
26	4611.5
27	5419.0
28	6487.5
29	7556.0
30	8839.0
31	10122.0
32	11402.5
33	12683.0
34	14085.5
35	15488.0
36	16350.5
37	17213.0
38	18440.5
39	19668.0
40	21183.5
41	22699.0
42	24892.5
43	27086.0
44	27915.5
45	28745.0
46	28698.0
47	28651.0
48	29330.5
49	30010.0
50	29336.0
51	28662.0
52	27064.0
53	25466.0
54	24751.5
55	24037.0
56	23873.5
57	23710.0
58	23691.0
59	23672.0
60	22563.5
61	21455.0
62	18918.5
63	16382.0
64	13811.0
65	11240.0
66	9489.0
67	7738.0
68	6489.0
69	5240.0
70	4393.0
71	3546.0
72	2895.0
73	2244.0
74	1784.5
75	999.0
76	673.0
77	529.5
78	386.0
79	352.0
80	318.0
81	252.5
82	187.0
83	140.5
84	94.0
85	60.5
86	27.0
87	20.5
88	14.0
89	14.0
90	14.0
91	13.5
92	13.0
93	8.5
94	4.0
95	2.0
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	446485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.919759745935323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.55056501775636	19.24626351269762
2	7.164882776322125	4.1441337699805185
3	4.2533912655981	3.690211605196705
4	2.908101026924953	3.364063320623098
5	2.2775298841697245	3.293280853218817
6	1.8601896773293145	3.227774313013964
7	1.6102219138360845	3.2597061620045817
8	1.4506335876749066	3.356157986795401
9	1.3161839101275847	3.4257350216119926
>10	10.539194145776904	50.89486763608285
>50	0.05202421607218714	0.9961321571487235
>100	0.015529616737966311	0.7872947121197981
>500	0.001552961673796631	0.3143789495059091
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	892	0.19978274746072097	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	508	0.11377761850902045	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2397168997838674E-4	0.0	0.0	0.011870499568854496	0.0
2	2.2397168997838674E-4	0.0	0.0	0.06696753530353763	0.0
3	2.2397168997838674E-4	0.0	0.0	0.09093250613122501	0.0
4	2.2397168997838674E-4	0.0	0.0	0.11915293906850175	0.0
5	2.2397168997838674E-4	0.0	0.0	0.19821494563087225	0.0
6	2.2397168997838674E-4	0.0	0.0	0.26025510375488536	0.0
7	2.2397168997838674E-4	0.0	0.0	0.3256548372285743	0.0
8	2.2397168997838674E-4	0.0	0.0	0.49005005767271015	0.0
9	2.2397168997838674E-4	0.0	0.0	0.5444751783374582	0.0
10	2.2397168997838674E-4	0.0	0.0	0.7010313896323505	0.0
11	2.2397168997838674E-4	0.0	0.0	0.9194037873612775	0.0
12	4.4794337995677347E-4	0.0	0.0	1.101492771313706	0.0
13	4.4794337995677347E-4	0.0	0.0	1.1769712308364222	0.0
14	4.4794337995677347E-4	0.0	0.0	1.2047437203937423	0.0
15	4.4794337995677347E-4	0.0	0.0	1.2708153689373662	0.0
16	4.4794337995677347E-4	0.0	0.0	1.4036305810945497	0.0
17	4.4794337995677347E-4	0.0	0.0	1.5395813969114305	0.0
18	4.4794337995677347E-4	0.0	0.0	1.707336192705242	0.0
19	4.4794337995677347E-4	0.0	0.0	1.8143946605149108	0.0
20	4.4794337995677347E-4	0.0	0.0	1.926828448884061	0.0
21	4.4794337995677347E-4	0.0	0.0	2.0708422455401636	0.0
22	4.4794337995677347E-4	0.0	0.0	2.2262785983851643	0.0
23	4.4794337995677347E-4	0.0	0.0	2.366932819691591	0.0
24	4.4794337995677347E-4	0.0	0.0	2.4892213624197903	0.0
25	4.4794337995677347E-4	0.0	0.0	2.5949360000895885	0.0
26	4.4794337995677347E-4	0.0	0.0	2.7044581564890198	0.0
27	4.4794337995677347E-4	0.0	0.0	2.8142042845784294	0.0
28	4.4794337995677347E-4	0.0	0.0	2.92932573322732	0.0
29	4.4794337995677347E-4	0.0	0.0	3.0471348421559514	0.0
30	4.4794337995677347E-4	0.0	0.0	3.177262394033394	0.0
31	4.4794337995677347E-4	0.0	0.0	3.32777136969887	0.0
32	4.4794337995677347E-4	0.0	0.0	3.4679776476253403	0.0
33	4.4794337995677347E-4	0.0	0.0	3.602584633302351	0.0
34	4.4794337995677347E-4	0.0	0.0	3.7488381468582372	0.0
35	4.4794337995677347E-4	0.0	0.0	3.892180028444405	0.0
36	4.4794337995677347E-4	0.0	0.0	4.042689004109881	0.0
37	4.4794337995677347E-4	0.0	0.0	4.197005498504989	0.0
38	4.4794337995677347E-4	0.0	0.0	4.35512951162973	0.0
39	4.4794337995677347E-4	0.0	0.0	4.515269269964277	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCT	25	3.8869606E-5	45.0	9
CCGATGT	20	7.028647E-4	45.0	11
CTAACGG	25	3.8869606E-5	45.0	2
CATACGG	25	3.8869606E-5	45.0	2
TACTCGT	20	7.028647E-4	45.0	31
ATGCGAT	20	7.028647E-4	45.0	14
AGTACGG	65	0.0	44.999996	2
AGGCGAT	180	0.0	42.5	7
CGTTTTA	205	0.0	41.70732	1
TTTCGAC	45	1.9237632E-8	40.0	24
ATAGCGG	40	3.4518962E-7	39.375	2
ACACGCG	120	0.0	39.374996	36
CTATGCG	35	6.2397066E-6	38.57143	1
CGAACGG	35	6.2397066E-6	38.57143	2
TAAGGCG	35	6.2397066E-6	38.57143	5
CTACGAA	90	0.0	37.5	11
TACGAAT	90	0.0	37.5	12
TATGCGG	60	1.546141E-10	37.499996	2
TCAGCGT	30	1.1388041E-4	37.499996	33
CGCTAGA	30	1.1388041E-4	37.499996	12
>>END_MODULE