FastQCFastQC Report
Sat 14 Jan 2017
SRR2934612.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934612.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567082
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85261.5034862682998225No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47940.8453803859053893No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18080.3188251434536804No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8960.15800184100359385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA302.1635478E-645.00000424
TAATTCG207.03026E-445.041
GCGATCG253.888298E-545.09
CGATCGT207.03026E-445.010
CGTTTTA15850.044.574131
CGTTATT7850.042.707011
CGTTTTT34950.041.974251
CGTATGG451.9254003E-840.02
GTTTTAT18450.040.02
GACCGAT1900.039.0789459
CGGTTTT4200.038.0357131
CGAAGGA1250.037.8000034
GTTATTT9000.037.7500042
TACGGGT601.546141E-1037.5000044
CGGTCTA301.1391924E-437.50000431
CGATCAC301.1391924E-437.50000410
ATAAGGA2800.036.9642834
CTCGATC552.744855E-936.8181816
ACGACCA2200.036.8181828
GCGAGAC2150.036.62790321
Produced by FastQC (version 0.11.5)