##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934612.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	567082
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.821676582928042	31.0	31.0	34.0	30.0	34.0
2	32.0097340419904	33.0	31.0	34.0	30.0	34.0
3	31.427714157740855	33.0	31.0	34.0	28.0	34.0
4	35.46011335221361	37.0	35.0	37.0	33.0	37.0
5	31.9147830472489	37.0	35.0	37.0	0.0	37.0
6	33.86267065433218	37.0	35.0	37.0	19.0	37.0
7	20.982988350891052	32.0	0.0	37.0	0.0	37.0
8	28.212791095467676	35.0	17.0	37.0	17.0	37.0
9	34.919531919546024	37.0	32.0	39.0	32.0	39.0
10	36.389716125710216	37.0	35.0	39.0	32.0	39.0
11	36.79482156019764	39.0	37.0	39.0	33.0	39.0
12	37.08567544023616	39.0	37.0	39.0	34.0	39.0
13	36.858089306308436	39.0	37.0	39.0	33.0	39.0
14	38.20089863547071	40.0	38.0	41.0	33.0	41.0
15	38.38299399381395	40.0	38.0	41.0	34.0	41.0
16	38.4593674283437	40.0	38.0	41.0	34.0	41.0
17	38.43404128503462	40.0	38.0	41.0	34.0	41.0
18	38.272955233987325	40.0	38.0	41.0	34.0	41.0
19	38.14048585566108	40.0	37.0	41.0	34.0	41.0
20	37.99815546957935	40.0	36.0	41.0	34.0	41.0
21	37.876615727531465	40.0	36.0	41.0	33.0	41.0
22	37.791575116120775	39.0	35.0	41.0	33.0	41.0
23	37.68257500678914	39.0	35.0	41.0	33.0	41.0
24	37.48324228242124	39.0	35.0	41.0	33.0	41.0
25	37.31711286903834	39.0	35.0	41.0	33.0	41.0
26	37.18260851164382	39.0	35.0	41.0	33.0	41.0
27	37.11082876903164	39.0	35.0	41.0	33.0	41.0
28	37.004031163041674	39.0	35.0	41.0	32.0	41.0
29	36.93176648174338	39.0	35.0	41.0	32.0	41.0
30	36.71883784003019	39.0	35.0	41.0	31.0	41.0
31	36.52019990054348	39.0	35.0	40.0	31.0	41.0
32	36.30382554903876	39.0	35.0	40.0	30.0	41.0
33	35.99161849609051	39.0	35.0	40.0	29.0	41.0
34	35.57666263432802	38.0	35.0	40.0	26.0	41.0
35	35.21516288649613	38.0	34.0	40.0	24.0	41.0
36	34.93343996106383	38.0	34.0	40.0	23.0	41.0
37	34.79621112996004	38.0	34.0	40.0	22.0	41.0
38	34.582855742203066	38.0	34.0	40.0	21.0	41.0
39	34.45474728522507	38.0	34.0	40.0	20.0	41.0
40	34.300623190296996	38.0	33.0	40.0	18.0	41.0
41	34.130090533644164	38.0	33.0	40.0	18.0	41.0
42	33.996778243710786	38.0	33.0	40.0	18.0	41.0
43	33.86543392313634	38.0	33.0	40.0	18.0	41.0
44	33.706167714722035	38.0	33.0	40.0	17.0	41.0
45	33.570525955681894	37.0	33.0	40.0	17.0	41.0
46	33.445353582021646	37.0	33.0	40.0	17.0	41.0
47	33.36226154242244	37.0	33.0	40.0	15.0	41.0
48	33.24097044166452	37.0	33.0	40.0	15.0	41.0
49	33.10691575468803	36.0	32.0	40.0	15.0	41.0
50	32.943244539590395	36.0	32.0	40.0	15.0	41.0
51	31.2633851894435	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	14.0
9	22.0
10	26.0
11	21.0
12	15.0
13	17.0
14	19.0
15	32.0
16	67.0
17	140.0
18	270.0
19	546.0
20	965.0
21	1707.0
22	2839.0
23	4638.0
24	7494.0
25	10758.0
26	13009.0
27	13055.0
28	12923.0
29	12733.0
30	13819.0
31	16272.0
32	20769.0
33	29631.0
34	38825.0
35	50439.0
36	62321.0
37	98746.0
38	95888.0
39	59054.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.0923429063169	18.698001347247843	23.286226683266264	20.923429063168992
2	38.195534331895566	22.028912926172932	24.17410533220945	15.60144740972205
3	29.303522241933265	22.089574347272528	33.547705622819976	15.059197787974227
4	25.716210354058145	23.464155095735713	34.03052821285105	16.789106337355094
5	20.934714908954966	34.82812009550647	29.152045030524686	15.085119965013877
6	24.03902786545861	31.665614496668915	31.10220391407239	13.193153723800085
7	45.506646305119894	41.86431591903816	10.409429324154178	2.2196084516877628
8	79.08750409993617	3.883036315735643	14.744075812669067	2.285383771659125
9	73.75176782193756	6.180058615861551	16.212999178249355	3.855174383951528
10	38.527232393198865	28.366268017676454	21.553849355119716	11.55265023400496
11	29.765889236477268	23.78809413806116	30.18117309313292	16.26484353232866
12	26.396711586684113	20.964163912802732	34.221153201829715	18.417971298683437
13	23.44175974550418	21.994879047474615	35.41357334565372	19.149787861367493
14	19.218384642785345	23.20846015214731	37.2931956930391	20.279959512028242
15	19.024056485658157	22.336452223840645	38.11371194994727	20.525779340553925
16	23.471208749351945	22.59461594619473	35.1483559696834	18.785819334769926
17	23.51370701238974	22.463417988932818	33.93918339852085	20.08369160015659
18	24.306184996173393	21.572717878543138	33.97480434928282	20.14629277600065
19	23.383214420489455	23.70521370807044	32.50588098370253	20.405690887737578
20	25.12440881565629	23.764111715765974	33.05677133113024	18.054708137447495
21	25.14398270444133	23.094014622223945	33.66373822480699	18.098264448527726
22	24.837501454816056	20.068349903541286	34.41001477740432	20.68413386423833
23	23.53557333860006	22.23629034249015	34.54544492683598	19.68269139207381
24	22.51067746816157	22.22288840061931	35.622890516715394	19.643543614503724
25	22.484049925760296	23.760761230298264	33.72016745373685	20.03502139020459
26	21.895069848804933	24.143421938978843	33.0856913109568	20.87581690125943
27	21.47908062678766	23.38039295904296	34.507707879989134	20.63281853418024
28	19.77826839857375	24.98844964220342	34.46415156890891	20.769130390313922
29	21.299388800914155	23.978190103018612	33.9864428777496	20.735978218317634
30	22.379655852240063	23.38991539142487	34.441050853315744	19.789377903019318
31	23.68105494443484	23.37510271883079	33.09750618076398	19.84633615597039
32	23.98542009797525	23.59306061557235	32.688746953703344	19.732772332749054
33	23.665889589159946	23.539276506748582	31.893976532494417	20.900857371597052
34	21.702857787762618	24.840675598943363	33.74538426541488	19.71108234787914
35	22.3047107825676	26.004704786962023	32.564073626036446	19.126510804433927
36	23.045520753612355	27.02184163842266	30.420820974744395	19.51181663322059
37	22.91943669522221	26.196387823983123	30.06408244310347	20.820093037691198
38	22.6180693444687	26.956419001132108	29.94681545173361	20.478696202665574
39	22.42127240857583	25.745130333884692	29.61635177981315	22.217245477726326
40	23.996000578399595	24.527493378382665	30.230901351127347	21.245604692090385
41	21.37715533203311	25.05052179402626	30.775090727619634	22.79723214632099
42	22.111969697504065	25.51747366342081	30.634723020656622	21.7358336184185
43	22.382477313686557	24.46824268800632	30.539498696837498	22.609781301469628
44	22.602551306512993	25.394916431838784	29.372295364691524	22.630236896956703
45	22.36572488634801	25.374813519032518	29.72709414158799	22.532367453031483
46	22.38864926060076	25.579722156584054	29.104256527274714	22.927372055540467
47	21.370278019757283	26.498989564119473	30.597867680511815	21.53286473561143
48	22.10544506790905	25.554152662225217	31.20412920882694	21.13627306103879
49	22.80640189602209	25.493138558444812	30.599983776596684	21.100475768936413
50	21.733012156972006	26.090759361080057	30.681629817204563	21.49459866474337
51	22.094335563463485	25.19935388532875	29.70558049805848	23.00073005314928
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	570.0
1	1013.5
2	1457.0
3	11950.0
4	22443.0
5	15147.0
6	7851.0
7	6756.5
8	5662.0
9	5433.0
10	5204.0
11	5173.0
12	5142.0
13	4909.5
14	4677.0
15	4501.0
16	4325.0
17	4093.5
18	3862.0
19	3716.5
20	3571.0
21	3595.0
22	3619.0
23	3697.5
24	3776.0
25	4269.5
26	5289.0
27	5815.0
28	6784.0
29	7753.0
30	8610.5
31	9468.0
32	10729.0
33	11990.0
34	13399.5
35	14809.0
36	15936.0
37	17063.0
38	18342.5
39	19622.0
40	21166.5
41	22711.0
42	24520.0
43	26329.0
44	27902.0
45	29475.0
46	30899.0
47	32323.0
48	32990.0
49	33657.0
50	33823.5
51	33990.0
52	33337.0
53	32684.0
54	32728.0
55	32772.0
56	32997.5
57	33223.0
58	31542.0
59	29861.0
60	27882.5
61	25904.0
62	23266.5
63	20629.0
64	17982.5
65	15336.0
66	13481.0
67	11626.0
68	9897.0
69	8168.0
70	6891.5
71	5615.0
72	4768.5
73	3922.0
74	3235.0
75	1954.5
76	1361.0
77	1035.0
78	709.0
79	563.0
80	417.0
81	295.0
82	173.0
83	134.5
84	96.0
85	73.0
86	50.0
87	41.0
88	32.0
89	24.5
90	17.0
91	12.5
92	8.0
93	6.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	567082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.149286896457646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.12607634802823	30.502351821537378
2	7.518182309841827	6.1873568161510795
3	3.6942169797815465	4.560431830763884
4	2.4847002594953325	4.0897457531870485
5	1.8766138176883944	3.86106601889582
6	1.5716373500234144	3.880305372798115
7	1.3642232188210552	3.9295768835453564
8	1.1466107503584562	3.7745771780050146
9	0.994294510823936	3.6823059076920446
>10	5.181530462220558	30.915817252886963
>50	0.031553230623256656	0.9032551478554128
>100	0.008476987331422865	0.6079192016247132
>500	4.7094374063460354E-4	0.1736358318953596
>1k	9.418874812692071E-4	1.2794015202825493
>5k	4.7094374063460354E-4	1.6522534628792815
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8526	1.5034862682998225	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4794	0.8453803859053893	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1808	0.3188251434536804	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	896	0.15800184100359385	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0160470619769275	0.0
2	0.0	0.0	0.0	0.06154312780162305	0.0
3	0.0	0.0	0.0	0.10439407352023164	0.0
4	0.0	0.0	0.0	0.13578283211246345	0.0
5	0.0	0.0	0.0	0.24246934305797047	0.0
6	0.0	0.0	0.0	0.3682007187673035	0.0
7	0.0	0.0	0.0	0.48282259003107136	0.0
8	0.0	0.0	0.0	0.7402809470235345	0.0
9	0.0	0.0	0.0	0.9018096148352442	0.0
10	0.0	0.0	0.0	1.1672033321459683	0.0
11	0.0	0.0	0.0	1.4170790115009821	0.0
12	0.0	0.0	0.0	1.6352132495829528	0.0
13	0.0	0.0	0.0	1.7369622029970975	0.0
14	0.0	0.0	0.0	1.8096148352442856	0.0
15	0.0	0.0	0.0	1.8697472323226623	0.0
16	0.0	0.0	0.0	1.9744939885237056	0.0
17	0.0	0.0	0.0	2.108337065891705	0.0
18	0.0	0.0	0.0	2.2518789169820237	0.0
19	0.0	0.0	0.0	2.3541568944173856	0.0
20	0.0	0.0	0.0	2.4809463181691536	0.0
21	0.0	0.0	0.0	2.610909886048226	0.0
22	0.0	0.0	0.0	2.7428132086717616	0.0
23	0.0	0.0	0.0	2.8904109105914135	0.0
24	0.0	0.0	0.0	3.011733752790602	0.0
25	0.0	0.0	0.0	3.1263556240543697	0.0
26	0.0	0.0	0.0	3.243975298105036	0.0
27	0.0	0.0	0.0	3.371293745878021	0.0
28	1.763413404057967E-4	0.0	0.0	3.4846812277589483	0.0
29	1.763413404057967E-4	0.0	0.0	3.6232855213179045	0.0
30	1.763413404057967E-4	1.763413404057967E-4	0.0	3.8065041739995276	0.0
31	3.526826808115934E-4	1.763413404057967E-4	0.0	3.954983582621208	0.0
32	3.526826808115934E-4	1.763413404057967E-4	0.0	4.106989818051005	0.0
33	3.526826808115934E-4	1.763413404057967E-4	0.0	4.259348736161614	0.0
34	3.526826808115934E-4	1.763413404057967E-4	0.0	4.410120582208569	0.0
35	3.526826808115934E-4	1.763413404057967E-4	0.0	4.590165090762888	0.0
36	3.526826808115934E-4	1.763413404057967E-4	0.0	4.748872297128105	0.0
37	3.526826808115934E-4	1.763413404057967E-4	0.0	4.911106330301438	0.0
38	3.526826808115934E-4	1.763413404057967E-4	0.0	5.116014967852974	0.0
39	3.526826808115934E-4	1.763413404057967E-4	0.0	5.439424986157205	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	30	2.1635478E-6	45.000004	24
TAATTCG	20	7.03026E-4	45.0	41
GCGATCG	25	3.888298E-5	45.0	9
CGATCGT	20	7.03026E-4	45.0	10
CGTTTTA	1585	0.0	44.57413	1
CGTTATT	785	0.0	42.70701	1
CGTTTTT	3495	0.0	41.97425	1
CGTATGG	45	1.9254003E-8	40.0	2
GTTTTAT	1845	0.0	40.0	2
GACCGAT	190	0.0	39.078945	9
CGGTTTT	420	0.0	38.035713	1
CGAAGGA	125	0.0	37.800003	4
GTTATTT	900	0.0	37.750004	2
TACGGGT	60	1.546141E-10	37.500004	4
CGGTCTA	30	1.1391924E-4	37.500004	31
CGATCAC	30	1.1391924E-4	37.500004	10
ATAAGGA	280	0.0	36.964283	4
CTCGATC	55	2.744855E-9	36.81818	16
ACGACCA	220	0.0	36.81818	28
GCGAGAC	215	0.0	36.627903	21
>>END_MODULE