##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934611.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	388851
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0028777089425	33.0	31.0	34.0	30.0	34.0
2	32.20262002669403	33.0	31.0	34.0	30.0	34.0
3	32.10128043903706	33.0	31.0	34.0	30.0	34.0
4	35.84820663956117	37.0	35.0	37.0	35.0	37.0
5	32.12167642618895	37.0	35.0	37.0	0.0	37.0
6	33.987123602613856	37.0	35.0	37.0	19.0	37.0
7	20.784539579427594	32.0	0.0	37.0	0.0	37.0
8	28.112837565031334	35.0	17.0	37.0	17.0	37.0
9	34.881975358170614	37.0	32.0	39.0	32.0	39.0
10	36.27232024605826	37.0	35.0	39.0	32.0	39.0
11	36.72095481302607	38.0	37.0	39.0	33.0	39.0
12	36.995936746980206	39.0	37.0	39.0	33.0	39.0
13	36.7074149224253	39.0	35.0	39.0	33.0	39.0
14	37.975831359569604	40.0	37.0	41.0	33.0	41.0
15	38.200050919246706	40.0	37.0	41.0	33.0	41.0
16	38.30594752231575	40.0	37.0	41.0	34.0	41.0
17	38.30577264813515	40.0	37.0	41.0	34.0	41.0
18	38.206400395009915	40.0	37.0	41.0	34.0	41.0
19	38.18232433502807	40.0	37.0	41.0	34.0	41.0
20	38.15073125695961	40.0	37.0	41.0	34.0	41.0
21	38.057345872840756	40.0	37.0	41.0	33.0	41.0
22	38.07279652103248	40.0	37.0	41.0	34.0	41.0
23	38.032971498080244	40.0	37.0	41.0	34.0	41.0
24	37.90311198891092	40.0	36.0	41.0	33.0	41.0
25	37.76598748620937	40.0	36.0	41.0	33.0	41.0
26	37.64379929587425	40.0	36.0	41.0	33.0	41.0
27	37.609613965246325	40.0	36.0	41.0	33.0	41.0
28	37.522138299760066	40.0	36.0	41.0	33.0	41.0
29	37.5160948538129	40.0	36.0	41.0	33.0	41.0
30	37.3828252981219	40.0	36.0	41.0	33.0	41.0
31	37.235457797459695	40.0	35.0	41.0	32.0	41.0
32	37.149748875533305	39.0	35.0	41.0	32.0	41.0
33	37.03718390848937	39.0	35.0	41.0	31.0	41.0
34	36.84800090523105	39.0	35.0	41.0	31.0	41.0
35	36.73835736567477	39.0	35.0	41.0	31.0	41.0
36	36.61764789083736	39.0	35.0	41.0	30.0	41.0
37	36.52342928268154	39.0	35.0	41.0	30.0	41.0
38	36.402979547435905	39.0	35.0	41.0	30.0	41.0
39	36.310483964294804	39.0	35.0	41.0	30.0	41.0
40	36.23389678822994	39.0	35.0	41.0	30.0	41.0
41	36.08851719553248	39.0	35.0	40.0	29.0	41.0
42	36.00916032104842	39.0	35.0	40.0	29.0	41.0
43	35.9342010178706	39.0	35.0	40.0	28.0	41.0
44	35.81719476097528	39.0	35.0	40.0	28.0	41.0
45	35.71170705488735	38.0	35.0	40.0	28.0	41.0
46	35.59683014830873	38.0	35.0	40.0	27.0	41.0
47	35.54122015887834	38.0	35.0	40.0	27.0	41.0
48	35.41522074007782	38.0	34.0	40.0	27.0	41.0
49	35.33168231533415	38.0	34.0	40.0	26.0	41.0
50	35.177443288046064	38.0	34.0	40.0	26.0	41.0
51	33.407351401950876	36.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	16.0
9	21.0
10	18.0
11	15.0
12	8.0
13	13.0
14	11.0
15	22.0
16	45.0
17	95.0
18	167.0
19	288.0
20	471.0
21	779.0
22	1269.0
23	1802.0
24	2579.0
25	3399.0
26	4119.0
27	4725.0
28	5400.0
29	6492.0
30	7688.0
31	9739.0
32	13202.0
33	19525.0
34	26961.0
35	33935.0
36	43856.0
37	74184.0
38	77250.0
39	50747.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3452684961592	20.193596004639307	25.43056337774623	12.030572121455261
2	32.98847116247611	24.02642657470342	26.46592139405582	16.519180868764643
3	32.383097896109305	23.927931264160307	27.19164924354058	16.497321596189803
4	28.453057855065307	25.87520669870979	26.544100439499964	19.12763500672494
5	22.63463383146758	38.029476586147396	22.608659872290417	16.727229710094612
6	26.47415076726047	34.77553098744763	24.557735482228413	14.192582763063488
7	49.64806571154504	43.19958030196656	4.618992879020499	2.533361107467899
8	85.2907669004323	4.694085909512898	6.965392913995334	3.049754276059468
9	79.82826326793553	7.735096476542429	8.298551373148069	4.138088882373968
10	41.21552985590882	30.983075779668816	15.728646705293286	12.072747659129075
11	33.53546731267246	26.349424329627542	23.29735554235427	16.817752815345724
12	29.860795008885148	24.720008435107534	27.073094835811144	18.346101720196167
13	25.45267981823372	26.74520574718851	29.000054005261656	18.802060429316114
14	21.333621361395497	28.45562953419176	30.262748456349602	19.94800064806314
15	20.38132858087031	26.225726563645203	34.18353045253838	19.209414402946116
16	24.88485306711311	25.844346549192366	30.558491555891585	18.712308827802936
17	25.420790997065712	24.541791071644408	28.123882926879446	21.91353500441043
18	26.051881054697045	24.372060249298574	29.824019997376887	19.752038698627494
19	25.99427544226452	26.04519468896827	26.993115614978485	20.96741425378873
20	26.12131639111125	27.781849603061325	27.444702469583465	18.65213153624396
21	25.285777842926983	26.53792840959648	29.314313194514096	18.861980552962446
22	25.738136201269896	23.68542192253588	29.857194658108117	20.719247218086103
23	22.669865835499973	25.88292173608914	29.51824734924174	21.928965079169142
24	22.497048998202395	26.33399425486883	31.229956975808214	19.938999771120557
25	23.400994211150287	27.274200143499694	28.490861538224156	20.833944107125866
26	21.77312132410615	28.694024189213863	27.26828528150886	22.264569205171135
27	21.019619340055705	27.911719398947156	30.266348807126636	20.802312453870506
28	19.78598486309666	27.742245744513966	30.613011153372373	21.858758239017
29	21.72374508487827	27.22045204975685	29.84973678864141	21.206066076723477
30	23.363447695904085	26.443547785655685	29.588968525219173	20.604035993221054
31	24.2447621325392	26.748548930052902	28.104852501343704	20.9018364360642
32	25.60055136800471	27.253626710488078	27.665609706545695	19.480212214961515
33	24.51761728785576	27.414870991716622	27.290401721996343	20.777109998431275
34	21.793180421292472	28.226492924024882	29.409208154280176	20.57111850040247
35	21.987085027426957	28.831866190391693	28.48417517249538	20.696873609685973
36	24.87996687677285	28.47285978433899	26.943996543663257	19.703176795224906
37	22.661122126470037	29.624457697164207	27.056121753576562	20.658298422789194
38	22.55259726733376	30.09018878696467	26.918279752398732	20.43893419330283
39	22.38543812411438	28.357648559473937	27.678982438003246	21.577930878408438
40	24.45589698882091	26.7860954452991	27.41409948797869	21.343908077901304
41	21.0116471347637	26.903106845552667	28.40625329496388	23.678992724719752
42	21.52701163170469	26.921880103175766	28.13982733746345	23.411280927656094
43	21.691341927884974	26.691971989270957	28.649534140326242	22.967151942517827
44	22.239881085557194	27.58588765362568	27.656351661690472	22.51787959912666
45	21.37888291402105	28.120539744015062	26.94785406235293	23.55272327961096
46	22.21647880550648	28.372049962582068	27.529310712843735	21.882160519067714
47	21.418229604655767	27.994013130993622	28.548724318569324	22.03903294578129
48	21.869816459260747	27.7754204052452	28.243723174172114	22.111039961321946
49	22.311116597359913	27.377067308557777	28.69839604372883	21.613420050353476
50	20.664470452692676	28.77631792126033	28.258896081018182	22.300315545028816
51	20.98695901514976	28.485975347883898	27.598746049257944	22.9283195877084
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	399.0
1	825.0
2	1251.0
3	2095.5
4	2940.0
5	2107.5
6	1275.0
7	1176.5
8	1078.0
9	1088.5
10	1099.0
11	1094.5
12	1090.0
13	1137.5
14	1185.0
15	1149.5
16	1114.0
17	1191.0
18	1268.0
19	1293.5
20	1319.0
21	1578.0
22	1837.0
23	2064.0
24	2291.0
25	2744.5
26	3936.0
27	4674.0
28	5619.0
29	6564.0
30	7528.5
31	8493.0
32	10124.5
33	11756.0
34	12822.5
35	13889.0
36	14656.0
37	15423.0
38	17013.0
39	18603.0
40	20402.5
41	22202.0
42	23716.0
43	25230.0
44	26092.5
45	26955.0
46	27316.0
47	27677.0
48	27620.5
49	27564.0
50	26635.5
51	25707.0
52	24777.5
53	23848.0
54	23132.5
55	22417.0
56	21058.5
57	19700.0
58	18226.5
59	16753.0
60	15426.5
61	14100.0
62	12894.5
63	11689.0
64	10158.5
65	8628.0
66	7297.0
67	5966.0
68	4886.5
69	3807.0
70	3164.0
71	2521.0
72	1999.0
73	1477.0
74	1209.0
75	682.0
76	423.0
77	320.5
78	218.0
79	172.5
80	127.0
81	97.5
82	68.0
83	50.0
84	32.0
85	26.5
86	21.0
87	23.5
88	26.0
89	15.5
90	5.0
91	2.5
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	388851.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.474142791994375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.23105452816165	22.482191503878703
2	7.544758486446773	4.900191288399678
3	3.96268451680071	3.860543485146345
4	2.7172869644517137	3.529662595617196
5	2.0757813206294133	3.370460950553712
6	1.7329665140662722	3.3765961218919713
7	1.4472658138750834	3.2899101688425985
8	1.243092637041046	3.229469423915822
9	1.1343044494937953	3.3152008196210474
>10	8.87111654751691	47.36260356972036
>50	0.032544341643662975	0.6333364539810409
>100	0.005556351012332704	0.29849556927555776
>500	0.0015875288606664868	0.35133804915594574
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	855	0.21987856531164893	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	508	0.13064129962376334	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.015687242671357408	0.0
2	0.0	0.0	0.0	0.09335195229020884	0.0
3	0.0	0.0	0.0	0.12061175103060041	0.0
4	0.0	0.0	0.0	0.1561009229756385	0.0
5	0.0	0.0	0.0	0.2682261328889472	0.0
6	0.0	0.0	0.0	0.3448621708572178	0.0
7	0.0	0.0	0.0	0.43127058950600616	0.0
8	0.0	0.0	0.0	0.6570640168084948	0.0
9	0.0	0.0	0.0	0.7385862451170243	0.0
10	0.0	0.0	0.0	0.9522927805251883	0.0
11	0.0	0.0	0.0	1.2408351785131067	0.0
12	0.0	0.0	0.0	1.4632854229512076	0.0
13	0.0	0.0	0.0	1.5607520618437396	0.0
14	0.0	0.0	0.0	1.6049849428187146	0.0
15	0.0	0.0	0.0	1.6836783240881468	0.0
16	0.0	0.0	0.0	1.8600955121627565	0.0
17	0.0	0.0	0.0	2.073544879658275	0.0
18	0.0	0.0	0.0	2.2846797359399873	0.0
19	0.0	0.0	0.0	2.41480669973846	0.0
20	0.0	0.0	0.0	2.571421958539389	0.0
21	0.0	0.0	0.0	2.7619833818094848	0.0
22	0.0	0.0	0.0	2.9741469097417776	0.0
23	0.0	0.0	0.0	3.1665085084003897	0.0
24	0.0	0.0	0.0	3.328009957541578	0.0
25	0.0	0.0	0.0	3.4815392013907642	0.0
26	0.0	0.0	0.0	3.6219528816950453	0.0
27	0.0	0.0	0.0	3.8107141295766245	0.0
28	0.0	0.0	0.0	3.9827594631362655	0.0
29	0.0	0.0	0.0	4.164062841551134	0.0
30	0.0	0.0	0.0	4.3965426345823975	0.0
31	0.0	0.0	0.0	4.582989371250171	0.0
32	0.0	0.0	0.0	4.780494328161686	0.0
33	0.0	0.0	0.0	4.963083546139781	0.0
34	0.0	0.0	0.0	5.172161059120332	0.0
35	0.0	0.0	0.0	5.396154311034303	0.0
36	0.0	0.0	0.0	5.601117137412531	0.0
37	0.0	0.0	0.0	5.802479613013725	0.0
38	0.0	0.0	0.0	6.06813406677622	0.0
39	0.0	0.0	0.0	6.471630521716544	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	7.027523E-4	45.0	38
GTCGCAT	20	7.027523E-4	45.0	37
TGCCCCG	20	7.027523E-4	45.0	43
CAACGTA	20	7.027523E-4	45.0	34
CGCGACT	20	7.027523E-4	45.0	45
CGTTAAT	20	7.027523E-4	45.0	26
ACGATAG	20	7.027523E-4	45.0	2
CGAATAT	45	3.8380676E-10	45.0	14
CGTCGCA	20	7.027523E-4	45.0	36
ACCAACG	20	7.027523E-4	45.0	32
AATACCG	20	7.027523E-4	45.0	26
CGCTTAA	20	7.027523E-4	45.0	27
TAGCGAA	30	2.1617834E-6	44.999996	1
ACCGATA	30	2.1617834E-6	44.999996	10
TCGTCCC	65	0.0	44.999996	38
CGTCCCC	60	0.0	44.999996	39
AGGCGAT	230	0.0	43.043476	7
AAGGCGA	140	0.0	41.785713	6
TACGAAT	50	1.0768417E-9	40.5	12
TTACGGG	50	1.0768417E-9	40.5	3
>>END_MODULE