##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934610.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329161
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.915609686445237	31.0	31.0	34.0	30.0	34.0
2	32.11205154924186	33.0	31.0	34.0	30.0	34.0
3	31.997970597974852	33.0	31.0	34.0	30.0	34.0
4	35.76733877950304	37.0	35.0	37.0	35.0	37.0
5	32.06454895932386	37.0	35.0	37.0	0.0	37.0
6	33.93566977861897	37.0	35.0	37.0	19.0	37.0
7	20.759959411959496	32.0	0.0	37.0	0.0	37.0
8	28.104717752103074	35.0	17.0	37.0	17.0	37.0
9	34.84448036067457	37.0	32.0	39.0	32.0	39.0
10	36.24864124243152	37.0	35.0	39.0	32.0	39.0
11	36.6630250849888	38.0	35.0	39.0	33.0	39.0
12	36.85017058521514	39.0	35.0	39.0	33.0	39.0
13	36.38189518199301	39.0	35.0	39.0	32.0	39.0
14	37.471298240070965	40.0	36.0	41.0	32.0	41.0
15	37.78561554983732	40.0	36.0	41.0	33.0	41.0
16	37.970263791883	40.0	36.0	41.0	33.0	41.0
17	37.98976792511871	40.0	36.0	41.0	33.0	41.0
18	37.929912717484754	40.0	36.0	41.0	33.0	41.0
19	37.90075373449467	40.0	36.0	41.0	33.0	41.0
20	37.83440930122342	40.0	36.0	41.0	33.0	41.0
21	37.734622266914975	40.0	36.0	41.0	33.0	41.0
22	37.766412181273	39.0	36.0	41.0	33.0	41.0
23	37.74951771321633	39.0	35.0	41.0	33.0	41.0
24	37.64073811903597	39.0	35.0	41.0	33.0	41.0
25	37.493639890509506	39.0	35.0	41.0	33.0	41.0
26	37.34038661931395	39.0	35.0	41.0	33.0	41.0
27	37.30574703564517	39.0	35.0	41.0	33.0	41.0
28	37.24394749074161	39.0	35.0	41.0	33.0	41.0
29	37.215906501681545	39.0	35.0	41.0	32.0	41.0
30	37.0532444609173	39.0	35.0	41.0	32.0	41.0
31	36.84833865494393	39.0	35.0	41.0	31.0	41.0
32	36.77802959645888	39.0	35.0	41.0	31.0	41.0
33	36.62376770030471	39.0	35.0	41.0	31.0	41.0
34	36.45910056173119	39.0	35.0	40.0	30.0	41.0
35	36.334459428668644	39.0	35.0	40.0	30.0	41.0
36	36.16422054860691	39.0	35.0	40.0	30.0	41.0
37	36.041839707620284	39.0	35.0	40.0	29.0	41.0
38	35.83532374734553	38.0	35.0	40.0	29.0	41.0
39	35.75381348337136	38.0	35.0	40.0	28.0	41.0
40	35.625927737490166	38.0	35.0	40.0	27.0	41.0
41	35.45582253061572	38.0	35.0	40.0	26.0	41.0
42	35.37554874362394	38.0	34.0	40.0	26.0	41.0
43	35.27768174236924	38.0	34.0	40.0	25.0	41.0
44	35.108803898396225	38.0	34.0	40.0	25.0	41.0
45	35.011887799587434	38.0	34.0	40.0	24.0	41.0
46	34.925993662675715	38.0	34.0	40.0	24.0	41.0
47	34.860855326117004	38.0	34.0	40.0	24.0	41.0
48	34.69933254547167	38.0	34.0	40.0	23.0	41.0
49	34.64491540613864	37.0	34.0	40.0	24.0	41.0
50	34.47761733619718	37.0	34.0	40.0	23.0	41.0
51	32.4962222134457	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	19.0
10	20.0
11	7.0
12	5.0
13	7.0
14	10.0
15	19.0
16	40.0
17	71.0
18	160.0
19	294.0
20	453.0
21	751.0
22	1182.0
23	1732.0
24	2579.0
25	3502.0
26	4397.0
27	4931.0
28	5331.0
29	6549.0
30	7653.0
31	9649.0
32	12974.0
33	19157.0
34	25987.0
35	31394.0
36	35762.0
37	57837.0
38	59325.0
39	37354.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.270870485871654	20.40855386877546	26.22789455615945	13.092681089193434
2	32.1110338102023	23.93053855104341	26.04591673983248	17.912510898921806
3	31.404692536479107	24.140466215620926	27.868125324689135	16.58671592321083
4	27.845643925009345	25.85998948842664	27.638450484717204	18.655916101846817
5	22.396031121548422	36.83152013756186	23.25761557414153	17.514833166748186
6	27.424877187759183	33.159456922296386	25.68925237193957	13.726413518004868
7	49.096642676380256	42.87597862444214	5.182570231588797	2.844808467588809
8	84.9708805113607	4.094652768705891	7.711727695565392	3.2227390243680145
9	79.52673615647055	6.767812711712505	9.229829779348101	4.475621352468853
10	43.01025941712414	29.806386540325253	15.486950155091279	11.696403887459327
11	36.46483028062255	22.79279744562691	23.93053855104341	16.811833722707124
12	34.28504591977786	21.087552899644855	27.403003393476137	17.224397787101147
13	25.681049699083424	26.340605357256784	29.266225342613495	18.712119601046297
14	19.598312072207825	27.55885417774281	33.01089740279073	19.831936347258637
15	19.354966110808995	24.322444031947892	38.45564936307764	17.86694049416547
16	25.01724080313281	22.752999292139712	33.72118811159281	18.508571793134667
17	24.404470760509295	22.90459683862912	28.226308706073926	24.464623694787658
18	25.085596410267314	23.46602422522717	31.367628607277293	20.080750757228223
19	26.099386014746585	25.834773864461464	27.17423996159934	20.891600159192613
20	26.545368375961914	27.546398267109407	28.027014135939552	17.88121922098912
21	24.164466628792596	25.496641461169457	31.194157266504842	19.144734643533106
22	22.317346222669148	23.428960296025352	31.19658768809185	23.057105793213655
23	21.41930544627097	25.460488940062763	31.18777740983895	21.932428203827307
24	22.240180337281757	25.33592983372878	31.71426748612381	20.70962234286565
25	21.78569150051191	24.7028050103141	31.20873979602687	22.302763693147124
26	19.666971482040704	27.810402811997776	29.2288576107133	23.293768095248222
27	19.967736153432515	28.219625046709666	30.282749171378143	21.52988962847968
28	18.53166079821121	26.91023541671097	32.50324309380515	22.05486069127266
29	20.35052755338573	25.965712827461335	30.94929229161413	22.734467327538805
30	22.87239375260131	26.065056309830144	30.51424682754032	20.548303110028225
31	24.997797430436776	25.821710348431314	28.64829065411759	20.53220156701432
32	27.094947457323315	25.166711730733592	26.714890281655478	21.02345053028761
33	24.838908619186356	25.287929007385447	28.407375114305765	21.465787259122436
34	20.21624676070373	25.288536612782192	31.392540428544084	23.10267619796999
35	20.839953700468765	27.55794276964768	28.49000944826392	23.112094081619635
36	25.470210626410783	26.853120509416367	27.56705685059895	20.109612013573905
37	21.889592023356354	29.47676061258776	27.910657702461712	20.722989661594173
38	21.26892311057507	28.448996083983218	27.528777710603624	22.75330309483809
39	21.525028785305672	26.58000188357673	27.667919346459634	24.227049984657963
40	23.87251223565368	24.18664422577401	28.073495948791017	23.867347589781293
41	18.96002260292076	25.104128374868225	28.543782525876395	27.39206649633462
42	20.695647418740375	24.797287649508903	28.82874945695268	25.678315474798048
43	20.8445107409444	24.690956705077454	29.911806076661573	24.552726477316572
44	21.466698667217564	25.18007904946212	30.146037957109133	23.207184326211188
45	20.239031963081896	27.11530223811448	27.57829755043884	25.06736824836478
46	20.937778169345698	26.713371268163606	28.249093908452096	24.0997566540386
47	19.28418008208749	26.0021691512664	31.08539590048639	23.628254866159722
48	20.972411676960515	26.005510980948532	30.45470149865871	22.56737584343224
49	21.722804341948166	25.04124121630448	30.5294369624591	22.70651747928825
50	19.851987325351423	26.810588131643787	29.28992195308679	24.047502589918004
51	19.10098705496702	27.720781015976986	27.598044725833255	25.58018720322274
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	332.0
1	620.5
2	909.0
3	1914.0
4	2919.0
5	2047.5
6	1176.0
7	1091.5
8	1007.0
9	1014.0
10	1021.0
11	1030.5
12	1040.0
13	985.5
14	931.0
15	906.0
16	881.0
17	872.5
18	864.0
19	978.0
20	1092.0
21	1233.5
22	1375.0
23	1554.5
24	1734.0
25	1997.0
26	2769.5
27	3279.0
28	4091.0
29	4903.0
30	5835.0
31	6767.0
32	7542.5
33	8318.0
34	8897.5
35	9477.0
36	10625.5
37	11774.0
38	13223.5
39	14673.0
40	16687.0
41	18701.0
42	20206.5
43	21712.0
44	22995.5
45	24279.0
46	25171.5
47	26064.0
48	25557.5
49	25051.0
50	24598.5
51	24146.0
52	22600.5
53	21055.0
54	19533.5
55	18012.0
56	17702.5
57	17393.0
58	16496.0
59	15599.0
60	14000.0
61	12401.0
62	10748.5
63	9096.0
64	7894.0
65	6692.0
66	5860.0
67	5028.0
68	3998.0
69	2968.0
70	2377.0
71	1786.0
72	1416.0
73	1046.0
74	825.0
75	491.5
76	379.0
77	286.5
78	194.0
79	138.0
80	82.0
81	64.5
82	47.0
83	43.0
84	39.0
85	29.5
86	20.0
87	20.0
88	20.0
89	16.0
90	12.0
91	7.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	329161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.40727464857777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.26139987521842	22.445732083011738
2	9.034990445915916	5.855988336561465
3	3.914671859050273	3.8059153828590224
4	2.5168647994329327	3.262589152342427
5	1.8209559866638656	2.9506110391393907
6	1.4788439766040413	2.8755181747321252
7	1.2417556734390927	2.816934200889923
8	1.0674783915671493	2.7675252333550926
9	0.9340412061218822	2.7242756909892614
>10	8.67920884207494	48.71293482282732
>50	0.03783959737270218	0.8283832112298344
>100	0.009957788782290047	0.48760694669586274
>500	0.001991557756458009	0.4659857253665294
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	891	0.2706882042526302	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	553	0.16800289220168854	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05984913157998669	0.0
2	0.0	0.0	0.0	0.28922016885353974	0.0
3	0.0	0.0	0.0	0.3833990053499655	0.0
4	0.0	0.0	0.0	0.496717411844052	0.0
5	0.0	0.0	0.0	0.8230015098994109	0.0
6	0.0	0.0	0.0	1.0356633987623078	0.0
7	0.0	0.0	0.0	1.254401341592716	0.0
8	0.0	0.0	0.0	1.7657012829587952	0.0
9	0.0	0.0	0.0	1.9367422021442395	0.0
10	0.0	0.0	0.0	2.473865372872242	0.0
11	0.0	0.0	0.0	3.2187895892891323	0.0
12	0.0	0.0	0.0	3.862243704448583	0.0
13	0.0	0.0	0.0	4.115615154893805	0.0
14	0.0	0.0	0.0	4.233490601863526	0.0
15	0.0	0.0	0.0	4.459519809454948	0.0
16	0.0	0.0	0.0	4.861450779405823	0.0
17	0.0	0.0	0.0	5.337509607760336	0.0
18	0.0	0.0	0.0	5.879189818963972	0.0
19	0.0	0.0	0.0	6.220360249239734	0.0
20	0.0	0.0	0.0	6.564264903800876	0.0
21	0.0	0.0	0.0	6.988069668034791	0.0
22	0.0	0.0	0.0	7.3927348622710465	0.0
23	0.0	0.0	0.0	7.802564702379686	0.0
24	0.0	0.0	0.0	8.147076962337579	0.0
25	0.0	0.0	0.0	8.439335158174876	0.0
26	0.0	0.0	0.0	8.75285954289846	0.0
27	0.0	0.0	0.0	9.124714045710155	0.0
28	0.0	0.0	0.0	9.447656314083382	0.0
29	0.0	0.0	0.0	9.824979265465835	0.0
30	0.0	0.0	0.0	10.24210037033549	0.0
31	0.0	0.0	0.0	10.596638119339776	0.0
32	0.0	0.0	0.0	10.903478844699098	0.0
33	0.0	0.0	0.0	11.246168288466738	0.0
34	0.0	0.0	0.0	11.633516728895586	0.0
35	0.0	0.0	0.0	12.022687985514688	0.0
36	0.0	0.0	0.0	12.375706721027097	0.0
37	0.0	0.0	0.0	12.717180954001233	0.0
38	0.0	0.0	0.0	13.13612487506114	0.0
39	0.0	0.0	0.0	13.850668821640474	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACACGC	20	7.025943E-4	45.000004	9
TTTCCGT	20	7.025943E-4	45.000004	44
AAGCGAA	20	7.025943E-4	45.000004	1
CTGACGT	20	7.025943E-4	45.000004	32
GCGCAAA	20	7.025943E-4	45.000004	1
TAGTTAG	20	7.025943E-4	45.000004	2
AACGGGT	20	7.025943E-4	45.000004	19
ACGATAA	20	7.025943E-4	45.000004	1
CGCTAGC	20	7.025943E-4	45.000004	16
TCTCGCA	20	7.025943E-4	45.000004	28
TCGTCAT	20	7.025943E-4	45.000004	12
TTGCGTG	20	7.025943E-4	45.000004	1
TTGCGCA	20	7.025943E-4	45.000004	19
TTTGCGA	20	7.025943E-4	45.000004	13
TAAGGCG	20	7.025943E-4	45.000004	5
CGGTCTA	20	7.025943E-4	45.000004	31
CGACTTT	20	7.025943E-4	45.000004	14
CGCAAAG	20	7.025943E-4	45.000004	2
CCACGCA	20	7.025943E-4	45.000004	37
GCGTCAG	20	7.025943E-4	45.000004	2
>>END_MODULE