##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934608.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	377050
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.977339875348097	33.0	31.0	34.0	30.0	34.0
2	32.17751491844583	33.0	31.0	34.0	30.0	34.0
3	32.0558758785307	33.0	31.0	34.0	30.0	34.0
4	35.813194536533615	37.0	35.0	37.0	35.0	37.0
5	32.07361888343721	37.0	35.0	37.0	0.0	37.0
6	33.96236573398753	37.0	35.0	37.0	19.0	37.0
7	20.852369712239756	32.0	0.0	37.0	0.0	37.0
8	28.155589444370772	35.0	17.0	37.0	17.0	37.0
9	34.90788754807055	37.0	32.0	39.0	32.0	39.0
10	36.27526853202493	37.0	35.0	39.0	32.0	39.0
11	36.695732661450734	38.0	35.0	39.0	33.0	39.0
12	36.99537196658268	39.0	37.0	39.0	33.0	39.0
13	36.6670521151041	39.0	35.0	39.0	33.0	39.0
14	37.857915395836095	40.0	37.0	41.0	33.0	41.0
15	38.10635459488132	40.0	37.0	41.0	33.0	41.0
16	38.23280466781594	40.0	37.0	41.0	33.0	41.0
17	38.24326481898952	40.0	37.0	41.0	34.0	41.0
18	38.17850152499668	40.0	37.0	41.0	33.0	41.0
19	38.11670070282456	40.0	37.0	41.0	34.0	41.0
20	38.08068956371834	40.0	37.0	41.0	33.0	41.0
21	37.99829730804933	40.0	37.0	41.0	33.0	41.0
22	38.023368253547275	40.0	36.0	41.0	34.0	41.0
23	37.98009547805331	40.0	36.0	41.0	34.0	41.0
24	37.868733589709585	40.0	36.0	41.0	33.0	41.0
25	37.74174247447288	40.0	36.0	41.0	33.0	41.0
26	37.59090836759051	40.0	36.0	41.0	33.0	41.0
27	37.554576316138444	40.0	36.0	41.0	33.0	41.0
28	37.47626044291208	40.0	35.0	41.0	33.0	41.0
29	37.466996419573	40.0	35.0	41.0	33.0	41.0
30	37.2896061530301	39.0	35.0	41.0	32.0	41.0
31	37.1438111656279	39.0	35.0	41.0	32.0	41.0
32	37.04878928524069	39.0	35.0	41.0	31.0	41.0
33	36.901413605622594	39.0	35.0	41.0	31.0	41.0
34	36.73646996419573	39.0	35.0	41.0	31.0	41.0
35	36.60587985678292	39.0	35.0	41.0	30.0	41.0
36	36.4282482429386	39.0	35.0	41.0	30.0	41.0
37	36.31209388675242	39.0	35.0	41.0	30.0	41.0
38	36.12420633868187	39.0	35.0	41.0	29.0	41.0
39	35.98564911815409	39.0	35.0	40.0	29.0	41.0
40	35.871669539848824	39.0	35.0	40.0	28.0	41.0
41	35.7223524731468	39.0	35.0	40.0	27.0	41.0
42	35.63256332051452	39.0	35.0	40.0	27.0	41.0
43	35.5600556955311	38.0	35.0	40.0	26.0	41.0
44	35.418514785837424	38.0	34.0	40.0	26.0	41.0
45	35.31422357777483	38.0	34.0	40.0	26.0	41.0
46	35.23270653759448	38.0	34.0	40.0	26.0	41.0
47	35.1480970693542	38.0	34.0	40.0	25.0	41.0
48	35.048940458825086	38.0	34.0	40.0	25.0	41.0
49	34.95135127967113	38.0	34.0	40.0	24.0	41.0
50	34.79421827343854	38.0	34.0	40.0	24.0	41.0
51	32.938562524864075	36.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	25.0
10	20.0
11	15.0
12	6.0
13	8.0
14	9.0
15	13.0
16	46.0
17	80.0
18	151.0
19	256.0
20	470.0
21	851.0
22	1237.0
23	1936.0
24	2764.0
25	3761.0
26	4605.0
27	5140.0
28	5763.0
29	6857.0
30	8072.0
31	10253.0
32	13645.0
33	19315.0
34	26363.0
35	33356.0
36	41841.0
37	69633.0
38	72878.0
39	47663.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.33337753613579	20.47792070017239	26.642885558944435	12.545816204747382
2	32.077443309905846	23.745922291473278	26.48216416920833	17.694470229412545
3	31.82946558811829	23.595809574327014	28.211908234982097	16.362816602572604
4	27.687044158599655	26.064182469168546	27.409362153560537	18.839411218671266
5	22.671794191751758	36.707068028112985	23.536401007823894	17.084736772311366
6	26.985280466781592	34.41506431507758	24.696724572337885	13.902930645802943
7	49.96764354860098	42.72483755470097	4.86858506829333	2.438933828404721
8	86.38774698315873	4.114838880785042	6.905980639172523	2.5914334968837025
9	80.6282986341334	7.13008884763294	8.428590372629625	3.8130221456040316
10	41.781726561464	30.249834239490784	15.37674048534677	12.591698713698449
11	33.00596737833178	25.234584272642884	24.18963002254343	17.569818326481897
12	30.327012332581887	23.25049728152765	28.03421296910224	18.388277416788224
13	25.308844980771784	25.36957963134863	29.62445299031959	19.697122397560005
14	19.983026123856252	26.908632807319982	32.683994165230075	20.424346903593687
15	19.96286964593555	24.89828935154489	35.142554037926004	19.996286964593555
16	25.31335366662246	24.80997215223445	31.00782389603501	18.868850285108078
17	25.316536268399414	24.017504309773237	27.722583211775625	22.943376210051717
18	26.588781328736243	23.932104495425012	29.062723776687445	20.416390399151307
19	25.77456570746585	25.831056889006764	27.670070282455907	20.724307121071476
20	25.861822039517307	27.336692746320118	28.23445166423551	18.567033549927064
21	25.078371568757458	25.710648455112057	29.98594350881846	19.22503646731203
22	25.044423816469962	22.619811696061532	30.880519824956902	21.455244662511603
23	22.31587322636255	25.262697255005968	30.649250762498344	21.77217875613314
24	22.772311364540514	25.940326216682134	30.798567829200373	20.48879458957698
25	23.347831852539453	26.642885558944435	28.181938734915796	21.82734385360032
26	21.75547009680414	27.03805861291606	28.41029041241215	22.796180877867656
27	20.950271847235115	26.53839013393449	30.670733324492772	21.84060469433762
28	19.05476727224506	27.120275825487333	30.94470229412545	22.880254608142156
29	21.44065773770057	26.585863943774036	30.258321177562657	21.715157140962738
30	23.405914334968834	26.194934358838353	29.83609600848694	20.563055297705873
31	25.16987136984485	25.960747911417588	28.456703354992708	20.412677363744862
32	25.605622596472617	25.992308712372363	27.607479114175838	20.79458957697918
33	24.294125447553373	26.187243071210712	28.312690624585603	21.20594085665031
34	21.292666755072272	26.68982893515449	30.484285903726295	21.533218406046945
35	22.125977987004376	27.67192680015913	29.452592494364144	20.74950271847235
36	24.460946824028643	27.99787826548203	27.29001458692481	20.251160323564513
37	22.31799496088052	28.51266410290412	28.107943243601646	21.061397692613713
38	22.83012863015515	28.12040843389471	27.78623524731468	21.263227688635457
39	21.815674313751494	27.18101047606418	27.85280466781594	23.150510542368387
40	25.109136719268	25.426866463333774	27.372231799496088	22.091765017902134
41	21.239358175308315	25.768465720726695	27.922821906908897	25.069354197056093
42	21.79286566768333	26.544490120673654	28.516377138310567	23.14626707333245
43	21.558679220262565	25.010741280997216	30.106351942713168	23.32422755602705
44	22.09759978782655	26.082482429386022	29.172788754807055	22.647129027980373
45	21.578305264553773	26.54369447022941	27.51783583079167	24.360164434425144
46	21.85970030499934	26.758520090173715	28.101578040047738	23.280201564779208
47	20.538655350749238	27.072536798833045	29.955708791937408	22.43309905848031
48	21.206471290279804	27.297175440922956	29.63479644609468	21.86155682270256
49	22.165495292401538	26.003713035406445	29.725235379923088	22.10555629226893
50	20.892719798435223	26.628033417318658	29.236175573531366	23.243071210714756
51	20.83622861689431	27.081023736904918	27.82575255271184	24.25699509348893
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	292.0
1	661.5
2	1031.0
3	2310.0
4	3589.0
5	2507.5
6	1426.0
7	1259.5
8	1093.0
9	1116.0
10	1139.0
11	1166.5
12	1194.0
13	1152.5
14	1111.0
15	1111.0
16	1111.0
17	1084.5
18	1058.0
19	1220.5
20	1383.0
21	1570.5
22	1758.0
23	2050.0
24	2342.0
25	2667.5
26	3368.0
27	3743.0
28	4592.0
29	5441.0
30	6739.0
31	8037.0
32	9264.5
33	10492.0
34	11670.5
35	12849.0
36	13829.0
37	14809.0
38	15986.0
39	17163.0
40	18949.5
41	20736.0
42	21892.0
43	23048.0
44	24159.0
45	25270.0
46	25708.0
47	26146.0
48	26465.5
49	26785.0
50	26502.5
51	26220.0
52	24804.5
53	23389.0
54	22535.0
55	21681.0
56	20634.5
57	19588.0
58	18796.0
59	18004.0
60	16793.0
61	15582.0
62	13976.0
63	12370.0
64	10476.0
65	8582.0
66	7101.5
67	5621.0
68	4721.0
69	3821.0
70	3075.0
71	2329.0
72	1973.0
73	1617.0
74	1319.0
75	827.5
76	634.0
77	461.0
78	288.0
79	211.0
80	134.0
81	88.0
82	42.0
83	40.0
84	38.0
85	29.0
86	20.0
87	19.0
88	18.0
89	12.5
90	7.0
91	4.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	377050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.66294173138129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.84306251525817	25.606521313381307
2	7.811698016787314	5.7279965842524
3	4.1646553907458035	4.580655537665891
4	2.87790968558647	4.220505404433384
5	2.24459193712741	4.114667170081525
6	1.880739892828042	4.137207426158327
7	1.5846380152743558	4.066824385353478
8	1.3360657308288555	3.9187280030938942
9	1.2464081042388702	4.1127288929308
>10	6.979458657953173	38.118965369666846
>50	0.024910709872205925	0.6100306127036004
>100	0.004396007624506928	0.29521076325902984
>500	7.326679374178213E-4	0.17782486376476594
>1k	7.326679374178213E-4	0.3121336732547704
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1162	0.3081819387349158	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	662	0.17557353136188836	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022543429253414668	0.0
2	0.0	0.0	0.0	0.12703885426336028	0.0
3	0.0	0.0	0.0	0.17663439862087257	0.0
4	0.0	0.0	0.0	0.22304734120143216	0.0
5	0.0	0.0	0.0	0.3755470096804137	0.0
6	0.0	0.0	0.0	0.4911815409096937	0.0
7	0.0	0.0	0.0	0.6145073597666092	0.0
8	0.0	0.0	0.0	0.922954515316271	0.0
9	0.0	0.0	0.0	1.0454846837289484	0.0
10	0.0	0.0	0.0	1.3321840604694337	0.0
11	0.0	0.0	0.0	1.7639570348760112	0.0
12	0.0	0.0	0.0	2.095212836493834	0.0
13	0.0	0.0	0.0	2.238164699641957	0.0
14	0.0	0.0	0.0	2.3057949874022015	0.0
15	0.0	0.0	0.0	2.4238164699641955	0.0
16	0.0	0.0	0.0	2.648189895239358	0.0
17	0.0	0.0	0.0	2.9118154090969366	0.0
18	0.0	0.0	0.0	3.198249569022676	0.0
19	0.0	0.0	0.0	3.3931839278610263	0.0
20	0.0	0.0	0.0	3.608274764620077	0.0
21	0.0	0.0	0.0	3.859965521814083	0.0
22	0.0	0.0	0.0	4.1392388277416785	0.0
23	0.0	0.0	0.0	4.4185121336692745	0.0
24	0.0	0.0	0.0	4.638111656279008	0.0
25	0.0	0.0	0.0	4.848959024002122	0.0
26	0.0	0.0	0.0	5.0351412279538525	0.0
27	0.0	0.0	0.0	5.294523272775494	0.0
28	0.0	0.0	0.0	5.508288025460814	0.0
29	0.0	0.0	0.0	5.766343986208725	0.0
30	0.0	0.0	0.0	6.046943376210051	0.0
31	0.0	0.0	0.0	6.276621137780135	0.0
32	0.0	0.0	0.0	6.488794589576979	0.0
33	0.0	0.0	0.0	6.72669407240419	0.0
34	0.0	0.0	0.0	6.991645670335499	0.0
35	0.0	0.0	0.0	7.264288555894444	0.0
36	0.0	0.0	0.0	7.510144543164037	0.0
37	0.0	0.0	0.0	7.788356981832648	0.0
38	0.0	0.0	0.0	8.136056225964726	0.0
39	0.0	0.0	0.0	8.71263758122265	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	25	3.8858023E-5	45.000004	1
CGTTATT	100	0.0	45.000004	1
TCGACGT	25	3.8858023E-5	45.000004	26
TTCGACG	25	3.8858023E-5	45.000004	25
CGACGGT	20	7.027251E-4	45.0	28
CCGTGAC	20	7.027251E-4	45.0	37
TACGGGT	20	7.027251E-4	45.0	4
TATGCGG	20	7.027251E-4	45.0	2
ATAAGCG	20	7.027251E-4	45.0	33
CGATCTA	20	7.027251E-4	45.0	10
ACGGTCT	20	7.027251E-4	45.0	30
CCCGACC	20	7.027251E-4	45.0	33
TACGAAT	50	1.0768417E-9	40.500004	12
CGTTTTA	230	0.0	40.108692	1
CGTTTTT	395	0.0	39.3038	1
GACCGAT	150	0.0	39.0	9
ACGGGTA	35	6.2371273E-6	38.571426	5
TTGACGG	65	9.094947E-12	38.07692	2
GCGATGT	95	0.0	37.894737	9
CTTCGAC	30	1.1384675E-4	37.500004	21
>>END_MODULE