##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934607.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	187640
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.521210829247494	31.0	31.0	34.0	30.0	34.0
2	31.711207631635045	31.0	31.0	34.0	30.0	34.0
3	31.348310594755915	31.0	31.0	34.0	28.0	34.0
4	35.30774355148156	37.0	35.0	37.0	33.0	37.0
5	31.698070773822213	35.0	35.0	37.0	0.0	37.0
6	33.666526327009166	36.0	35.0	37.0	17.0	37.0
7	21.063728416115968	32.0	0.0	37.0	0.0	37.0
8	28.2634779364741	35.0	17.0	37.0	17.0	37.0
9	34.81837028352164	37.0	32.0	39.0	31.0	39.0
10	35.99595502025154	37.0	35.0	39.0	32.0	39.0
11	36.33683116606267	37.0	35.0	39.0	32.0	39.0
12	36.685083137923684	38.0	35.0	39.0	33.0	39.0
13	36.27232466425069	38.0	35.0	39.0	32.0	39.0
14	37.56250266467704	39.0	36.0	41.0	33.0	41.0
15	37.841174589639735	40.0	36.0	41.0	33.0	41.0
16	37.97543700703475	40.0	36.0	41.0	33.0	41.0
17	37.99092410999787	40.0	36.0	41.0	33.0	41.0
18	37.88128330846301	39.0	36.0	41.0	33.0	41.0
19	37.75916115966745	39.0	36.0	41.0	33.0	41.0
20	37.66384033255169	39.0	36.0	41.0	33.0	41.0
21	37.55833510978469	39.0	35.0	40.0	33.0	41.0
22	37.55772223406523	39.0	35.0	40.0	33.0	41.0
23	37.511932423790235	39.0	35.0	40.0	33.0	41.0
24	37.35413557876785	39.0	35.0	41.0	33.0	41.0
25	37.2256022170113	39.0	35.0	40.0	33.0	41.0
26	37.0850778085696	39.0	35.0	40.0	32.0	41.0
27	37.05208377744617	39.0	35.0	40.0	32.0	41.0
28	37.011133020677896	39.0	35.0	40.0	32.0	41.0
29	37.022234065231295	39.0	35.0	40.0	32.0	41.0
30	36.86551907908761	39.0	35.0	40.0	32.0	41.0
31	36.701705393306334	38.0	35.0	40.0	31.0	41.0
32	36.50202515455127	38.0	35.0	40.0	31.0	41.0
33	36.2935621402686	38.0	35.0	40.0	30.0	41.0
34	36.060333617565554	38.0	35.0	40.0	30.0	41.0
35	35.79214453208271	38.0	35.0	40.0	29.0	41.0
36	35.50267000639523	38.0	35.0	40.0	27.0	41.0
37	35.31686207631635	38.0	35.0	40.0	25.0	41.0
38	35.1327915156683	38.0	34.0	40.0	25.0	41.0
39	35.01401620123641	38.0	34.0	40.0	24.0	41.0
40	34.81277979108932	38.0	34.0	40.0	23.0	41.0
41	34.671749094009805	38.0	34.0	40.0	23.0	41.0
42	34.532285227030485	38.0	34.0	40.0	22.0	41.0
43	34.33141121296099	38.0	33.0	40.0	21.0	41.0
44	34.08795565977403	38.0	33.0	40.0	18.0	41.0
45	33.8904018332978	38.0	33.0	40.0	18.0	41.0
46	33.55710935834577	37.0	33.0	40.0	15.0	41.0
47	33.43165636324878	37.0	33.0	40.0	15.0	41.0
48	33.31631848219996	37.0	33.0	40.0	15.0	41.0
49	33.129604561927096	37.0	32.0	40.0	14.0	41.0
50	32.8833937326796	37.0	32.0	40.0	13.0	41.0
51	30.831166062673205	35.0	27.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	6.0
10	6.0
11	2.0
12	1.0
13	2.0
14	2.0
15	7.0
16	13.0
17	23.0
18	50.0
19	99.0
20	229.0
21	411.0
22	764.0
23	1277.0
24	2023.0
25	2930.0
26	3439.0
27	3787.0
28	4056.0
29	4332.0
30	5308.0
31	6595.0
32	8257.0
33	11368.0
34	15527.0
35	19439.0
36	20457.0
37	30140.0
38	29549.0
39	17531.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.554252824557665	21.518332978043063	27.361436793860584	15.565977403538692
2	33.018546152206355	23.516307823491793	27.178639948838203	16.286506075463652
3	28.4193135791942	21.906309955233425	33.96823704966958	15.706139415902792
4	24.946706459177147	25.022916222553825	32.31453847793647	17.71583884033255
5	19.19633340439139	36.16179918993818	27.95832445107653	16.683542954593904
6	22.801108505649115	33.01694734598167	30.510552121082924	13.671392027286291
7	46.99850778085696	41.3227456832232	8.740140694947772	2.938605840972074
8	80.5979535280324	3.161372841611597	12.098699637603923	4.141973992752079
9	76.44105734384993	5.601684075890002	14.003410786612664	3.95384779364741
10	38.46248134726071	29.8113408654871	20.662438712428056	11.063739074824133
11	27.485077808569603	23.97090172671072	31.97612449371136	16.567895971008316
12	27.808569601364315	22.251652099765508	34.54007674269878	15.399701556171392
13	18.93519505435941	32.2777659347687	33.800362396077595	14.986676614794286
14	14.843316989980815	34.387657215945424	32.502664677041146	18.266361117032616
15	13.405990193988487	27.66947345981667	42.75207844809209	16.17245789810275
16	15.977936474099339	24.946706459177147	39.59976550842038	19.475591558303133
17	15.992858665529738	24.713813685781282	32.5149221914304	26.77840545725858
18	17.92634832658282	25.770091664890217	37.73235983798763	18.57120017053933
19	18.81368578128331	29.48411852483479	32.003837134939246	19.698358558942655
20	21.283841398422513	27.227670006395225	33.55308036665956	17.935408228522704
21	17.278298870176933	28.894159027925813	34.94244297591132	18.88509912598593
22	17.13920272862929	26.37177574078022	31.975058622894903	24.51396290769559
23	14.996802387550629	28.444361543380943	32.4701556171392	24.088680451929225
24	17.060861223619696	25.86442123214666	37.658814751652095	19.415902792581537
25	14.420699211255597	28.812619910466854	34.59550202515455	22.171178853123003
26	13.708164570454063	31.313685781283308	32.95139629076956	22.026753357493075
27	14.961095715199319	30.42261777872522	34.36900447665743	20.247282029418034
28	12.634299722873587	27.972713707098702	37.522916222553825	21.870070347473884
29	12.773928799829463	26.184715412492004	36.47836282242592	24.56299296525261
30	15.698145384779364	29.287998294606695	34.76284374333831	20.251012577275635
31	18.84672777659348	29.615220635259004	30.565444468130465	20.972607120017052
32	17.75261138350032	30.430611809848646	31.270518013216797	20.546258793434234
33	17.385951822639097	29.884353016414412	31.00778085696014	21.721914303986356
34	14.43348966105308	28.52430185461522	31.437326795992327	25.604881688339376
35	14.186740567043273	27.31187380089533	33.26103176295033	25.240353869111065
36	19.319974419100404	27.197825623534428	31.591878064378598	21.89032189298657
37	14.926454913664463	30.04849712214879	34.47345981667022	20.55158814751652
38	14.714346621189511	31.151673417181836	31.352057130675764	22.78192283095289
39	16.470368791302494	29.955233425708805	31.802920486037088	21.77147729695161
40	19.923790236623322	26.65263270091665	31.78107013429972	21.64250692816031
41	15.84470262204221	25.509486250266466	32.53197612449371	26.11383500319761
42	16.954807077382224	25.02078448092091	31.60200383713494	26.422404604561926
43	17.40300575570241	26.801321679812407	31.245470049030054	24.550202515455126
44	16.300895331485822	29.212854402046474	29.298124067363034	25.188126199104673
45	16.714453208271156	31.393626092517586	26.853016414410575	25.038904284800683
46	19.453741206565763	29.86623321253464	29.29599232573012	21.384033255169474
47	16.272116819441486	27.159454274141975	32.48987422724366	24.078554679172885
48	16.824770837774462	25.261671285440208	33.52963120869751	24.383926668087827
49	17.534640801534852	25.09646130888936	32.56821573225325	24.80068215732253
50	16.953208271157536	27.341185248347898	31.49594969089746	24.2096567895971
51	15.831912172244724	26.97452568748668	29.702622042208482	27.49094009806011
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	416.0
1	490.0
2	564.0
3	2398.0
4	4232.0
5	2971.0
6	1710.0
7	1543.5
8	1377.0
9	1408.0
10	1439.0
11	1469.5
12	1500.0
13	1423.0
14	1346.0
15	1332.5
16	1319.0
17	1268.0
18	1217.0
19	1199.5
20	1182.0
21	1200.0
22	1218.0
23	1306.0
24	1394.0
25	1670.5
26	2168.5
27	2390.0
28	2683.0
29	2976.0
30	3511.5
31	4047.0
32	4732.0
33	5417.0
34	6070.0
35	6723.0
36	7264.5
37	7806.0
38	8632.0
39	9458.0
40	10947.0
41	12436.0
42	14396.0
43	16356.0
44	17785.5
45	19215.0
46	19656.0
47	20097.0
48	19668.0
49	19239.0
50	17325.0
51	15411.0
52	12518.0
53	9625.0
54	7652.0
55	5679.0
56	4477.0
57	3275.0
58	2827.0
59	2379.0
60	2009.0
61	1639.0
62	1368.0
63	1097.0
64	908.5
65	720.0
66	540.5
67	361.0
68	272.0
69	183.0
70	143.0
71	103.0
72	88.5
73	74.0
74	54.0
75	28.5
76	23.0
77	15.5
78	8.0
79	6.0
80	4.0
81	3.5
82	3.0
83	2.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	187640.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.94702229896799
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.84597004391199	43.61399348740618
2	8.297996925319516	8.953044503339596
3	2.958035480534244	4.787316180885581
4	1.64399986070837	3.547555885805388
5	1.0159996302489867	2.740507735439266
6	0.8189996966586741	2.6509556939096104
7	0.639999762861317	2.4168257034889584
8	0.5429997988007791	2.3434577803392607
9	0.4329998395593344	2.102314680014128
>10	2.763998975847571	23.30240764773161
>50	0.021999991848280234	0.7638895527072106
>100	0.01499999444200925	1.3734906788083039
>500	9.999996294672833E-4	0.5060228816662172
>1k	9.999996294672833E-4	0.8982175884586904
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1665	0.8873374547004903	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	938	0.4998934129183543	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	381	0.20304839053506715	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	332	0.17693455553186954	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.152952462161586	0.0
2	0.0	0.0	0.0	0.7908761458111276	0.0
3	0.0	0.0	0.0	1.018439565124707	0.0
4	0.0	0.0	0.0	1.2865060754636537	0.0
5	0.0	0.0	0.0	2.085909187806438	0.0
6	0.0	0.0	0.0	2.6135152419526753	0.0
7	0.0	0.0	0.0	3.128330846301428	0.0
8	0.0	0.0	0.0	4.464399914730334	0.0
9	0.0	0.0	0.0	4.9030057557024085	0.0
10	0.0	0.0	0.0	6.226817309742059	0.0
11	0.0	0.0	0.0	8.093157109358346	0.0
12	0.0	0.0	0.0	9.383393732679599	0.0
13	0.0	0.0	0.0	9.89874227243658	0.0
14	0.0	0.0	0.0	10.084736729908336	0.0
15	0.0	0.0	0.0	10.4503304199531	0.0
16	0.0	0.0	0.0	11.376039224046046	0.0
17	0.0	0.0	0.0	12.652952462161586	0.0
18	0.0	0.0	0.0	14.079620549989341	0.0
19	0.0	0.0	0.0	14.876358985290983	0.0
20	0.0	0.0	0.0	15.581965465785547	0.0
21	0.0	0.0	0.0	16.631315284587508	0.0
22	0.0	0.0	0.0	17.610850564911534	0.0
23	0.0	0.0	0.0	18.872841611596673	0.0
24	0.0	0.0	0.0	19.703154977616713	0.0
25	0.0	0.0	0.0	20.457258580260074	0.0
26	0.0	0.0	0.0	21.13195480707738	0.0
27	0.0	0.0	0.0	21.809315710935834	0.0
28	0.0	0.0	0.0	22.479215519079087	0.0
29	0.0	0.0	0.0	23.160839906203368	0.0
30	0.0	0.0	0.0	23.945320827115754	0.0
31	0.0	0.0	0.0	24.73672990833511	0.0
32	0.0	0.0	0.0	25.377851204434023	0.0
33	0.0	0.0	0.0	26.004050309102535	0.0
34	5.329354082285227E-4	0.0	0.0	26.65902792581539	0.0
35	5.329354082285227E-4	0.0	0.0	27.41952675335749	0.0
36	5.329354082285227E-4	0.0	0.0	28.096354721807717	0.0
37	5.329354082285227E-4	0.0	0.0	28.74493711362183	0.0
38	5.329354082285227E-4	0.0	0.0	29.42975911319548	0.0
39	5.329354082285227E-4	0.0	0.0	30.112982306544446	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGATC	45	3.8198777E-10	45.000004	6
AGGTAGC	25	3.8782928E-5	45.0	7
GATACTA	35	1.2046803E-7	45.0	1
CGGAACA	20	7.018186E-4	45.0	30
GTCGAAG	20	7.018186E-4	45.0	1
ATAGGCG	20	7.018186E-4	45.0	5
CGTTGGA	35	1.2046803E-7	45.0	34
ACATTCG	20	7.018186E-4	45.0	2
CGCGCGG	20	7.018186E-4	45.0	2
AGGACCA	35	1.2046803E-7	45.0	7
CACGAGG	20	7.018186E-4	45.0	3
TACGGGT	35	1.2046803E-7	45.0	4
CGACAGG	20	7.018186E-4	45.0	3
AGGCGAT	55	1.8189894E-12	45.0	7
AGGCGAC	20	7.018186E-4	45.0	7
CATTCGG	20	7.018186E-4	45.0	3
ACGGGGT	40	6.7630026E-9	45.0	5
ACCTCAC	35	1.2046803E-7	45.0	29
AACGGAC	25	3.8782928E-5	45.0	16
ACAGGAC	20	7.018186E-4	45.0	5
>>END_MODULE