FastQCFastQC Report
Sat 14 Jan 2017
SRR2934606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790259
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74110.9377938119021739No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41780.5286874303234762No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.1054084800046567No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAG351.2112832E-745.0000042
CGTTTCA551.8189894E-1245.0000041
GCGAAGT207.031947E-445.03
TACGCGG253.889695E-545.02
TGCGACG207.031947E-445.01
CAATCGT207.031947E-445.026
TCGGTTG207.031947E-445.01
CGTAAAG600.044.9999962
CGTTTTA15700.043.566881
CGTTTTT31550.042.717911
CGTTATT7150.042.167831
CGGGTAT750.042.06
TCACGAC700.041.78571725
CGGTTTA1300.039.8076931
AGGCGAT4500.039.57
ACGTTAG403.456389E-739.3752
TGCGCAA403.456389E-739.3751
CGACGGT750.039.028
CACGACG750.039.026
TCACGGG1050.038.571433
Produced by FastQC (version 0.11.5)