##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934606.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	790259
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65049812782898	31.0	31.0	34.0	30.0	34.0
2	31.844132113648815	31.0	31.0	34.0	30.0	34.0
3	31.447152136198387	31.0	31.0	34.0	28.0	34.0
4	35.40380685319623	37.0	35.0	37.0	33.0	37.0
5	31.788864157194034	37.0	35.0	37.0	0.0	37.0
6	33.734061870855	37.0	35.0	37.0	18.0	37.0
7	21.0770190532471	32.0	0.0	37.0	0.0	37.0
8	28.261219422999297	35.0	17.0	37.0	17.0	37.0
9	34.80431731875246	37.0	32.0	39.0	31.0	39.0
10	36.062950247956685	37.0	35.0	39.0	32.0	39.0
11	36.50097120058108	38.0	35.0	39.0	32.0	39.0
12	36.920628553423626	39.0	37.0	39.0	33.0	39.0
13	36.74832554896559	39.0	37.0	39.0	33.0	39.0
14	38.026606466993734	40.0	37.0	41.0	33.0	41.0
15	38.195032261575	40.0	37.0	41.0	33.0	41.0
16	38.263980543087776	40.0	37.0	41.0	33.0	41.0
17	38.2564197307465	40.0	37.0	41.0	34.0	41.0
18	38.1210970074368	40.0	37.0	41.0	33.0	41.0
19	38.01862427381403	40.0	37.0	41.0	33.0	41.0
20	37.9209487016282	40.0	36.0	41.0	33.0	41.0
21	37.827500857313865	40.0	36.0	41.0	33.0	41.0
22	37.81691951625986	39.0	36.0	41.0	33.0	41.0
23	37.740714120307395	39.0	36.0	41.0	33.0	41.0
24	37.59577935841288	39.0	35.0	41.0	33.0	41.0
25	37.461357605544514	39.0	35.0	41.0	33.0	41.0
26	37.3279469136068	39.0	35.0	41.0	33.0	41.0
27	37.30587187238614	39.0	35.0	41.0	33.0	41.0
28	37.20379394603541	39.0	35.0	41.0	32.0	41.0
29	37.20409005148945	39.0	35.0	41.0	32.0	41.0
30	37.02359606154438	39.0	35.0	40.0	32.0	41.0
31	36.854456576894414	39.0	35.0	40.0	31.0	41.0
32	36.71057843061578	39.0	35.0	40.0	31.0	41.0
33	36.46813639578923	39.0	35.0	40.0	30.0	41.0
34	36.18158730239074	39.0	35.0	40.0	30.0	41.0
35	35.91490258257103	39.0	35.0	40.0	27.0	41.0
36	35.66686238309212	39.0	35.0	40.0	26.0	41.0
37	35.5563555745648	39.0	35.0	40.0	25.0	41.0
38	35.362725384968726	39.0	35.0	40.0	24.0	41.0
39	35.25653488286751	39.0	35.0	40.0	24.0	41.0
40	35.15315358635586	38.0	34.0	40.0	24.0	41.0
41	35.03010785071729	38.0	34.0	40.0	23.0	41.0
42	34.923452943908266	38.0	34.0	40.0	23.0	41.0
43	34.82870172943301	38.0	34.0	40.0	23.0	41.0
44	34.66189692240139	38.0	34.0	40.0	22.0	41.0
45	34.558509298850126	38.0	34.0	40.0	22.0	41.0
46	34.42333462826744	38.0	33.0	40.0	21.0	41.0
47	34.37716875100442	38.0	33.0	40.0	21.0	41.0
48	34.219273681160224	38.0	33.0	40.0	20.0	41.0
49	34.070904602162074	38.0	33.0	40.0	20.0	41.0
50	33.860216713760934	38.0	33.0	40.0	18.0	41.0
51	31.87382237975145	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	29.0
10	27.0
11	21.0
12	8.0
13	15.0
14	18.0
15	35.0
16	69.0
17	140.0
18	249.0
19	537.0
20	1002.0
21	1792.0
22	3041.0
23	5384.0
24	8182.0
25	11765.0
26	14309.0
27	15163.0
28	15630.0
29	16771.0
30	19012.0
31	23316.0
32	29871.0
33	40677.0
34	53627.0
35	69218.0
36	89510.0
37	141881.0
38	139923.0
39	89014.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.040261483893254	20.095690147154286	24.764918843062844	18.099129525889612
2	34.758477916733625	22.64118472551404	25.79280969909865	16.807527658653683
3	29.327979814212807	22.03277659602738	32.9774162647942	15.661827324965612
4	25.49480613317912	24.51133109524852	32.691560614937636	17.302302156634724
5	21.066764187437283	35.878363928787905	27.76671951853759	15.28815236523722
6	24.141578900082123	32.18020927316234	29.834522605879844	13.843689220875689
7	46.84932408235781	41.52220980716449	8.733339322930837	2.8951267875468676
8	80.56687744144642	3.564147956556015	12.329628640736772	3.5393459612608016
9	74.86393701305522	6.4461145017013415	14.393382422724702	4.296566062518744
10	35.07293178565508	31.45955946088561	21.269482536737954	12.198026216721354
11	25.870126123207708	24.427181468354046	32.12579673246366	17.576895675974587
12	23.752212882105738	22.040622125151373	35.21288590196379	18.9942790907791
13	21.951157784979355	23.138490039341534	36.79451926520293	18.115832910476186
14	18.312983464914666	25.432548063356442	36.50815745217707	19.74631101955182
15	17.61599678080224	25.547700184369937	38.4156333556467	18.420669679181128
16	21.075495502107536	25.164534665217353	35.39459847973892	18.365371352936187
17	21.4658738464225	24.83160584061681	33.477252394468145	20.225267918492545
18	21.839042642981603	24.51386191109497	34.56423780051857	19.08285764540486
19	20.904538891679817	26.670117012270662	32.66435434458829	19.76098975146123
20	21.675045776131626	27.129207006816753	32.73800108572	18.457746131331625
21	20.916686807742778	26.353385409087398	34.58866017343681	18.14126760973301
22	20.798244626128902	23.195053773509695	34.08920366613983	21.917497934221565
23	18.89380570167502	25.73751137285371	34.31052351191192	21.058159413559352
24	18.901777771591338	25.0462190243958	35.21946602316456	20.832537180848306
25	19.81426342502901	26.424121711995685	32.5531249881368	21.208489874838502
26	19.266468335064832	26.839048970021224	32.53325808374217	21.36122461117178
27	19.000859211979872	26.25177315285242	33.42549721040823	21.321870424759478
28	17.984103945668444	25.763199153695183	34.60169387504603	21.651003025590345
29	19.480322274089886	24.2397745549244	33.71907184859647	22.56083132238924
30	20.227292571169706	24.559922759500367	33.58481206794228	21.627972601387647
31	20.096069769531255	24.842488348756547	33.30439767215558	21.757044209556614
32	20.832790262432948	24.32480996736513	32.9122477567481	21.930152013453817
33	20.670185344298513	24.32329147785726	33.226322003292594	21.78020117455163
34	18.912027575769464	24.04642022425559	35.25489744501486	21.786654754960082
35	19.025914288859727	24.892471961723942	33.47548082337563	22.606132926040704
36	19.90182965331619	25.4525415085434	33.0246159803305	21.62101285780991
37	18.93847460136487	26.79273504003118	33.596959984005245	20.671830374598706
38	19.632171224876906	26.446139809859805	32.86302338853464	21.058665576728643
39	20.12492107018079	24.89297812489323	33.46232058097409	21.519780223951894
40	21.19824007066038	24.473621939136407	33.3455234296604	20.98261456054281
41	18.881657785612056	24.622813533284656	32.573498055700725	23.922030625402556
42	19.74491907083627	25.149476310930975	32.28966705852132	22.815937559711436
43	20.351049466061127	24.646982824618256	32.604753631404385	22.397214077916228
44	20.381419256218532	25.32536801225927	31.51295967524571	22.780253056276486
45	20.03241975099303	24.808701957206438	31.2038205196018	23.955057772198735
46	19.667855728311856	25.41331386292342	31.559526686820398	23.359303721944325
47	18.60719080706452	25.38851186762821	33.00563486148212	22.998662463825152
48	18.822310154012797	25.304109159149093	32.64372819543973	23.22985249139839
49	20.266140594412718	23.96189097498415	32.99234807828826	22.779620352314875
50	19.213068120704733	24.556379617315336	32.50465986467728	23.725892397302655
51	18.43332375841338	24.030223002838312	31.91093046710003	25.625522771648285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	647.0
1	934.5
2	1222.0
3	10718.5
4	20215.0
5	13971.0
6	7727.0
7	6626.0
8	5525.0
9	5432.0
10	5339.0
11	5174.5
12	5010.0
13	4855.0
14	4700.0
15	4574.5
16	4449.0
17	4383.0
18	4317.0
19	4435.0
20	4553.0
21	4563.0
22	4573.0
23	4921.0
24	5269.0
25	6247.0
26	8657.5
27	10090.0
28	11644.0
29	13198.0
30	15283.0
31	17368.0
32	20371.5
33	23375.0
34	25903.5
35	28432.0
36	29550.0
37	30668.0
38	33040.0
39	35412.0
40	38997.0
41	42582.0
42	45770.5
43	48959.0
44	53108.0
45	57257.0
46	62993.0
47	68729.0
48	69439.5
49	70150.0
50	65780.0
51	61410.0
52	54065.5
53	46721.0
54	41886.0
55	37051.0
56	33562.5
57	30074.0
58	27631.0
59	25188.0
60	22942.0
61	20696.0
62	17851.0
63	15006.0
64	12590.0
65	10174.0
66	8358.0
67	6542.0
68	5365.5
69	4189.0
70	3497.5
71	2806.0
72	2178.5
73	1551.0
74	1225.5
75	677.0
76	454.0
77	347.0
78	240.0
79	178.5
80	117.0
81	94.5
82	72.0
83	52.0
84	32.0
85	25.0
86	18.0
87	16.0
88	14.0
89	9.0
90	4.0
91	3.0
92	2.0
93	2.5
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	790259.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.86907367155216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.38691835298626	35.49666259147518
2	8.341537836918183	7.6523722715128
3	3.239367509212839	4.457603607879483
4	1.9475370332254647	3.573268786203799
5	1.391491310181225	3.1913208710013623
6	1.128509448703801	3.1058209824982415
7	0.9277161183894023	2.978743528648293
8	0.7556682766732828	2.7729443083185337
9	0.6694018923743257	2.7634360245474934
>10	4.171427030136952	30.08987476003672
>50	0.02764370954339371	0.8436912285642808
>100	0.010998019098391083	1.1334190180873884
>500	0.001188975037660934	0.36076346948824045
>1k	2.972437594152335E-4	0.569640882661326
>5k	2.972437594152335E-4	1.0104376690768517
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7411	0.9377938119021739	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4178	0.5286874303234762	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	833	0.1054084800046567	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10490231683536663	0.0
2	0.0	0.0	0.0	0.40644902493992474	0.0
3	0.0	0.0	0.0	0.5626003626659108	0.0
4	0.0	0.0	0.0	0.731026157247181	0.0
5	0.0	0.0	0.0	1.2022640678562344	0.0
6	0.0	0.0	0.0	1.7662563792376929	0.0
7	0.0	0.0	0.0	2.2657128865346676	0.0
8	0.0	0.0	0.0	3.3812965116499782	0.0
9	0.0	0.0	0.0	3.859747247421415	0.0
10	0.0	0.0	0.0	4.7480636095254845	0.0
11	0.0	0.0	0.0	5.8022749503643745	0.0
12	0.0	0.0	0.0	6.58530937325611	0.0
13	0.0	0.0	0.0	6.980622808471653	0.0
14	0.0	0.0	0.0	7.1846065656955505	0.0
15	0.0	0.0	0.0	7.434271548947876	0.0
16	0.0	0.0	0.0	7.880454382677072	0.0
17	0.0	0.0	0.0	8.459631589137231	0.0
18	0.0	0.0	0.0	9.084490021625822	0.0
19	0.0	0.0	0.0	9.511565195714317	0.0
20	0.0	0.0	0.0	9.935097227617781	0.0
21	0.0	0.0	0.0	10.467074718541642	0.0
22	0.0	0.0	0.0	11.051946260656317	0.0
23	0.0	0.0	0.0	11.662379042820138	0.0
24	0.0	0.0	0.0	12.15006725643112	0.0
25	0.0	0.0	0.0	12.573725829126907	0.0
26	0.0	0.0	0.0	12.971443539396578	0.0
27	0.0	0.0	0.0	13.395608275261655	0.0
28	1.2654079232251705E-4	0.0	0.0	13.84027261948298	0.0
29	1.2654079232251705E-4	0.0	0.0	14.304677327306617	0.0
30	1.2654079232251705E-4	0.0	0.0	14.837287522192092	0.0
31	1.2654079232251705E-4	0.0	0.0	15.303716882692889	0.0
32	1.2654079232251705E-4	0.0	0.0	15.75420210336105	0.0
33	1.2654079232251705E-4	0.0	0.0	16.209495874137467	0.0
34	1.2654079232251705E-4	0.0	0.0	16.633280987625575	0.0
35	1.2654079232251705E-4	0.0	0.0	17.101481919218887	0.0
36	1.2654079232251705E-4	0.0	0.0	17.51678879962139	0.0
37	1.2654079232251705E-4	0.0	0.0	17.990177903699927	0.0
38	1.2654079232251705E-4	0.0	0.0	18.46951442501762	0.0
39	1.2654079232251705E-4	0.0	0.0	19.090070470567245	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACTAG	35	1.2112832E-7	45.000004	2
CGTTTCA	55	1.8189894E-12	45.000004	1
GCGAAGT	20	7.031947E-4	45.0	3
TACGCGG	25	3.889695E-5	45.0	2
TGCGACG	20	7.031947E-4	45.0	1
CAATCGT	20	7.031947E-4	45.0	26
TCGGTTG	20	7.031947E-4	45.0	1
CGTAAAG	60	0.0	44.999996	2
CGTTTTA	1570	0.0	43.56688	1
CGTTTTT	3155	0.0	42.71791	1
CGTTATT	715	0.0	42.16783	1
CGGGTAT	75	0.0	42.0	6
TCACGAC	70	0.0	41.785717	25
CGGTTTA	130	0.0	39.807693	1
AGGCGAT	450	0.0	39.5	7
ACGTTAG	40	3.456389E-7	39.375	2
TGCGCAA	40	3.456389E-7	39.375	1
CGACGGT	75	0.0	39.0	28
CACGACG	75	0.0	39.0	26
TCACGGG	105	0.0	38.57143	3
>>END_MODULE