##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934604.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	521105
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.057021137774534	33.0	31.0	34.0	30.0	34.0
2	32.260049318275584	33.0	31.0	34.0	30.0	34.0
3	32.08061906909356	33.0	31.0	34.0	30.0	34.0
4	35.849189702651096	37.0	35.0	37.0	35.0	37.0
5	32.101121654944784	37.0	35.0	37.0	0.0	37.0
6	33.99975244912254	37.0	35.0	37.0	19.0	37.0
7	20.786864451502097	32.0	0.0	37.0	0.0	37.0
8	28.1195862638048	35.0	17.0	37.0	17.0	37.0
9	34.90676351215206	37.0	32.0	39.0	32.0	39.0
10	36.34992755778586	37.0	35.0	39.0	32.0	39.0
11	36.80169447616123	39.0	37.0	39.0	33.0	39.0
12	37.1466652594007	39.0	37.0	39.0	34.0	39.0
13	36.94514349315397	39.0	37.0	39.0	33.0	39.0
14	38.364538816553285	40.0	38.0	41.0	34.0	41.0
15	38.525778873739455	40.0	38.0	41.0	34.0	41.0
16	38.57942833018298	40.0	38.0	41.0	34.0	41.0
17	38.54322257510483	40.0	38.0	41.0	34.0	41.0
18	38.4331698985809	40.0	38.0	41.0	34.0	41.0
19	38.37188090691895	40.0	37.0	41.0	34.0	41.0
20	38.332794734266606	40.0	37.0	41.0	34.0	41.0
21	38.254973565788084	40.0	37.0	41.0	34.0	41.0
22	38.24430393106955	40.0	37.0	41.0	34.0	41.0
23	38.14551961696779	40.0	37.0	41.0	34.0	41.0
24	38.044518858963166	40.0	37.0	41.0	34.0	41.0
25	37.90311933295593	40.0	36.0	41.0	33.0	41.0
26	37.781995950912005	40.0	36.0	41.0	33.0	41.0
27	37.75791251283331	40.0	36.0	41.0	33.0	41.0
28	37.66398134732923	40.0	36.0	41.0	33.0	41.0
29	37.68255533913511	40.0	36.0	41.0	33.0	41.0
30	37.52985290872281	40.0	36.0	41.0	33.0	41.0
31	37.3979006150392	40.0	36.0	41.0	33.0	41.0
32	37.30834860536744	40.0	36.0	41.0	32.0	41.0
33	37.15861294748659	40.0	35.0	41.0	32.0	41.0
34	36.98224350178947	40.0	35.0	41.0	31.0	41.0
35	36.83873883382428	40.0	35.0	41.0	31.0	41.0
36	36.69593268151332	40.0	35.0	41.0	30.0	41.0
37	36.61554389230577	39.0	35.0	41.0	30.0	41.0
38	36.52816994655588	39.0	35.0	41.0	30.0	41.0
39	36.47076117097322	39.0	35.0	41.0	30.0	41.0
40	36.36324733019257	39.0	35.0	41.0	30.0	41.0
41	36.271843486437476	39.0	35.0	41.0	30.0	41.0
42	36.17363487205074	39.0	35.0	41.0	29.0	41.0
43	36.11298490707247	39.0	35.0	41.0	29.0	41.0
44	35.95722743017242	39.0	35.0	40.0	29.0	41.0
45	35.88965947361856	39.0	35.0	40.0	28.0	41.0
46	35.76516632924267	39.0	35.0	40.0	28.0	41.0
47	35.75815046871552	39.0	35.0	40.0	28.0	41.0
48	35.639554408420565	38.0	35.0	40.0	27.0	41.0
49	35.51133648688844	38.0	35.0	40.0	27.0	41.0
50	35.37408391782846	38.0	34.0	40.0	27.0	41.0
51	33.625810537223785	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	11.0
9	34.0
10	30.0
11	21.0
12	18.0
13	10.0
14	17.0
15	18.0
16	44.0
17	87.0
18	160.0
19	316.0
20	491.0
21	928.0
22	1389.0
23	2172.0
24	3393.0
25	4786.0
26	5758.0
27	6325.0
28	6939.0
29	7736.0
30	9704.0
31	12230.0
32	16391.0
33	24220.0
34	33944.0
35	44217.0
36	56463.0
37	99258.0
38	110344.0
39	73634.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.0142005929707	21.148329031577127	25.236372707995507	13.601097667456655
2	32.42225655098301	24.805557421249077	25.701154277928634	17.071031749839282
3	31.992016963951603	23.378205927788066	28.446474318995215	16.18330278926512
4	28.170138455781462	25.283963884437878	27.991287744312565	18.55460991546809
5	22.7196054537953	37.494554840195356	23.119332955930187	16.666506750079158
6	25.747210255130927	35.43450935991787	25.15730994713158	13.660970437819634
7	49.444161925139845	42.83973479433128	5.302961975033822	2.4131413054950537
8	85.66507709578684	4.5572389441667225	7.332303470509782	2.4453804895366575
9	79.15602421776802	7.938323370529932	9.013346638393415	3.8923057733086424
10	38.87700175588413	33.28676562305102	15.370222891739669	12.466009729325183
11	31.296955508007024	25.676207290277393	26.14252405945059	16.884313142264993
12	30.258585122000365	23.530766352270653	28.127728576774352	18.082919948954626
13	24.254420894061656	25.459360397616603	29.342646875389793	20.943571832931944
14	19.971790713963596	27.74757486495044	30.299843601577418	21.980790819508545
15	20.225674288291227	26.936989666190115	33.53853829842354	19.298797747095115
16	23.572408631657726	25.0592490956717	32.00429855787221	19.364043714798363
17	23.489315972788592	25.425777914239934	28.924688882279003	22.16021723069247
18	23.62307020658025	26.59272123660299	29.586743554561938	20.197465002254823
19	24.908799570144215	27.555483060035886	27.231939820189787	20.303777549630116
20	26.65144260753591	25.526909164179962	29.229425931434168	18.592222296849965
21	24.90476967213901	26.906093781483577	28.493297895817545	19.695838650559867
22	25.166137342762013	25.00647662179407	28.704195891423034	21.123190144020878
23	24.449391197551357	26.53879736329531	29.115245487953484	19.896565951199854
24	22.99881981558419	25.493326680803293	30.846182631139595	20.661670872472918
25	22.399900212049396	27.618042429068996	28.475643104556664	21.506414254324945
26	21.903647057694705	29.316740388213507	27.68079369800712	21.098818856084666
27	21.65590428032738	29.244969823739936	28.609973038063348	20.489152857869335
28	19.98848600569943	29.442434825994763	30.57387666593105	19.995202502374763
29	21.901536158739603	26.616900624634194	29.92467928728375	21.556883929342455
30	22.06311587875764	27.520173477514128	29.62454783584882	20.79216280787941
31	23.03835119601616	27.948110265685415	28.503852390593064	20.50968614770536
32	23.87100488385258	28.627244029514205	28.12600147762927	19.375749609003943
33	24.09188167451857	28.21677013269878	27.796893140537893	19.89445505224475
34	22.265186478732694	28.126960977154315	29.32019458650368	20.287657957609312
35	22.663378781627504	27.106245382408535	28.69863079417776	21.531745041786206
36	22.643421191506512	30.195258153347215	27.218890626649138	19.942430028497135
37	22.648794388846778	30.607267249402714	27.323667974784353	19.42027038696616
38	22.002859308584643	29.283733604551866	27.34976636186565	21.36364072499784
39	22.983275923278416	28.088580996152405	28.004912637568246	20.923230443000932
40	23.022039704090346	27.23501021866994	28.898398595292697	20.844551481947015
41	20.70791874958022	28.360311261645926	29.380834956486694	21.550935032287157
42	21.411615701250227	28.962109363755868	27.542625766400246	22.08364916859366
43	21.780639218583588	28.645858320300132	28.113336083898638	21.460166377217643
44	21.26711507277804	28.988015850932154	27.711113882998628	22.033755193291178
45	21.687951564463976	28.93927327505973	26.94639276153558	22.42638239894071
46	20.971205419253316	30.705903800577616	26.99110543940281	21.331785340766256
47	22.432139396091	27.999539440227977	28.790358948772322	20.777962214908705
48	21.78370961706374	27.295650588652958	29.560837067385652	21.35980272689765
49	21.47897256790858	27.75736176010593	28.666007810326132	22.097657861659357
50	20.090960554974522	29.705529595762847	28.37201715585151	21.831492693411118
51	20.47399276537358	28.241333320540008	27.925849876704312	23.3588240373821
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	320.0
1	837.0
2	1354.0
3	4079.5
4	6805.0
5	4796.5
6	2788.0
7	2364.5
8	1941.0
9	1887.5
10	1834.0
11	1812.0
12	1790.0
13	1791.0
14	1792.0
15	1727.5
16	1663.0
17	1734.0
18	1805.0
19	1955.0
20	2105.0
21	2230.0
22	2355.0
23	2733.5
24	3112.0
25	3946.0
26	5946.0
27	7112.0
28	8557.0
29	10002.0
30	11321.5
31	12641.0
32	14319.5
33	15998.0
34	17463.5
35	18929.0
36	20583.5
37	22238.0
38	24171.0
39	26104.0
40	27496.5
41	28889.0
42	30783.5
43	32678.0
44	34405.0
45	36132.0
46	36349.5
47	36567.0
48	35599.5
49	34632.0
50	33337.0
51	32042.0
52	30330.5
53	28619.0
54	27631.5
55	26644.0
56	25883.5
57	25123.0
58	24591.0
59	24059.0
60	22945.0
61	21831.0
62	19265.5
63	16700.0
64	13831.0
65	10962.0
66	9017.5
67	7073.0
68	5842.0
69	4611.0
70	3756.5
71	2902.0
72	2363.5
73	1825.0
74	1444.0
75	843.5
76	624.0
77	466.0
78	308.0
79	224.5
80	141.0
81	114.5
82	88.0
83	74.0
84	60.0
85	43.0
86	26.0
87	20.0
88	14.0
89	13.5
90	13.0
91	11.5
92	10.0
93	5.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	521105.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.22959839903334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.40811478543512	22.369956651685822
2	7.380597335730851	4.757473761511616
3	3.856198654754432	3.7285120196888393
4	2.730088245164797	3.5195859094233235
5	2.1100487867850624	3.4003012500225043
6	1.6722818843065212	3.2338184124706753
7	1.424277365926613	3.2132721258853447
8	1.2775501626976578	3.2939942942692175
9	1.123243438896739	3.2581516445991943
>10	8.963337150190386	46.846694156999256
>50	0.040998099195575136	0.8717038666288637
>100	0.011455351245822455	0.7516162630674191
>500	6.029132234643398E-4	0.10648544781823828
>1k	0.0012058264469286795	0.6484341959296737
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2136	0.40989819710039244	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1201	0.23047178591646594	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	548	0.10516114794523178	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8379981001909405E-4	0.0	0.0	0.01803859107089742	0.0
2	3.8379981001909405E-4	0.0	0.0	0.06793256637337965	0.0
3	3.8379981001909405E-4	0.0	0.0	0.09863655117490717	0.0
4	3.8379981001909405E-4	0.0	0.0	0.1220483395860719	0.0
5	3.8379981001909405E-4	0.0	0.0	0.20590859807524395	0.0
6	3.8379981001909405E-4	0.0	0.0	0.27518446378369044	0.0
7	3.8379981001909405E-4	0.0	0.0	0.340622331391946	0.0
8	3.8379981001909405E-4	0.0	0.0	0.5213920419109392	0.0
9	3.8379981001909405E-4	0.0	0.0	0.5893246082843189	0.0
10	3.8379981001909405E-4	0.0	0.0	0.7764270156686273	0.0
11	3.8379981001909405E-4	0.0	0.0	1.0155342973105228	0.0
12	3.8379981001909405E-4	0.0	0.0	1.2128073996603372	0.0
13	3.8379981001909405E-4	0.0	0.0	1.2974352577695474	0.0
14	3.8379981001909405E-4	0.0	0.0	1.3342800395313805	0.0
15	3.8379981001909405E-4	0.0	0.0	1.4010612064747028	0.0
16	3.8379981001909405E-4	0.0	0.0	1.5486322334270444	0.0
17	3.8379981001909405E-4	0.0	0.0	1.740724038341601	0.0
18	3.8379981001909405E-4	0.0	0.0	1.9278264457259093	0.0
19	3.8379981001909405E-4	0.0	0.0	2.0425825889216185	0.0
20	3.8379981001909405E-4	0.0	0.0	2.17326642423312	0.0
21	3.8379981001909405E-4	0.0	0.0	2.3434816399765883	0.0
22	3.8379981001909405E-4	0.0	0.0	2.5179186536302662	0.0
23	3.8379981001909405E-4	0.0	0.0	2.716918855125167	0.0
24	3.8379981001909405E-4	0.0	0.0	2.8696711795127663	0.0
25	3.8379981001909405E-4	0.0	0.0	3.02050450485027	0.0
26	3.8379981001909405E-4	0.0	0.0	3.1686512315176403	0.0
27	3.8379981001909405E-4	0.0	0.0	3.3087381621746097	0.0
28	3.8379981001909405E-4	0.0	0.0	3.4574605885570087	0.0
29	3.8379981001909405E-4	0.0	0.0	3.6190403085750473	0.0
30	3.8379981001909405E-4	0.0	0.0	3.7892555243185155	0.0
31	3.8379981001909405E-4	0.0	0.0	3.970792834457547	0.0
32	3.8379981001909405E-4	0.0	0.0	4.136594352385796	0.0
33	3.8379981001909405E-4	0.0	0.0	4.319666861764904	0.0
34	3.8379981001909405E-4	0.0	0.0	4.481438481687952	0.0
35	3.8379981001909405E-4	0.0	0.0	4.671227487742393	0.0
36	3.8379981001909405E-4	0.0	0.0	4.871379088667351	0.0
37	3.8379981001909405E-4	0.0	0.0	5.0803580852227475	0.0
38	3.8379981001909405E-4	0.0	0.0	5.300275376363689	0.0
39	3.8379981001909405E-4	0.0	0.0	5.525182065034878	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATG	25	3.88786E-5	45.0	1
GGTCGAC	20	7.029734E-4	45.0	8
CGACGGT	25	3.88786E-5	45.0	28
CGTGACA	20	7.029734E-4	45.0	26
TATGCGC	20	7.029734E-4	45.0	12
GTATGCG	20	7.029734E-4	45.0	11
TATTCGG	20	7.029734E-4	45.0	45
ACGCTAG	20	7.029734E-4	45.0	2
CGGTCTA	25	3.88786E-5	45.0	31
TAATGCG	20	7.029734E-4	45.0	1
CGCCCTG	20	7.029734E-4	45.0	32
GCGTAAA	30	2.1632095E-6	44.999996	1
CGTTTTA	485	0.0	44.072166	1
CGAATAT	160	0.0	43.59375	14
TACGAAT	165	0.0	42.272728	12
CCTATGC	145	0.0	41.89655	35
CGTTATT	235	0.0	41.17021	1
ACGGGTA	55	6.002665E-11	40.90909	5
AGTACGG	50	1.0786607E-9	40.5	2
GCGATCA	45	1.9248546E-8	40.0	9
>>END_MODULE