##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934603.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	573973
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.019216931806895	33.0	31.0	34.0	30.0	34.0
2	32.208706681324735	33.0	31.0	34.0	30.0	34.0
3	32.07922672320824	33.0	31.0	34.0	30.0	34.0
4	35.83162622632075	37.0	35.0	37.0	35.0	37.0
5	32.13989333993062	37.0	35.0	37.0	0.0	37.0
6	34.00433818315496	37.0	35.0	37.0	19.0	37.0
7	20.751648596710996	32.0	0.0	37.0	0.0	37.0
8	28.093532274166208	35.0	17.0	37.0	17.0	37.0
9	34.870392858200645	37.0	32.0	39.0	32.0	39.0
10	36.15090779531442	37.0	35.0	39.0	32.0	39.0
11	36.67519900761882	38.0	35.0	39.0	33.0	39.0
12	37.04989259076646	39.0	37.0	39.0	34.0	39.0
13	36.86586825512698	39.0	37.0	39.0	33.0	39.0
14	38.2541234518	40.0	38.0	41.0	34.0	41.0
15	38.430197936139855	40.0	38.0	41.0	34.0	41.0
16	38.49130011341997	40.0	38.0	41.0	34.0	41.0
17	38.48074560998514	40.0	38.0	41.0	34.0	41.0
18	38.37219346554629	40.0	38.0	41.0	34.0	41.0
19	38.319863477898785	40.0	37.0	41.0	34.0	41.0
20	38.313530427389445	40.0	37.0	41.0	34.0	41.0
21	38.230589243744916	40.0	37.0	41.0	34.0	41.0
22	38.217647868453746	40.0	37.0	41.0	34.0	41.0
23	38.15093392894788	40.0	37.0	41.0	34.0	41.0
24	38.05863342003892	40.0	37.0	41.0	34.0	41.0
25	37.92429957506712	40.0	37.0	41.0	33.0	41.0
26	37.80929242316276	40.0	36.0	41.0	33.0	41.0
27	37.76539488791285	40.0	36.0	41.0	33.0	41.0
28	37.70199817761463	40.0	36.0	41.0	33.0	41.0
29	37.7052213257418	40.0	36.0	41.0	33.0	41.0
30	37.57631456531927	40.0	36.0	41.0	33.0	41.0
31	37.462706433926336	40.0	36.0	41.0	33.0	41.0
32	37.36755039000092	40.0	36.0	41.0	32.0	41.0
33	37.23745890486138	40.0	36.0	41.0	32.0	41.0
34	37.1060973251355	40.0	36.0	41.0	31.0	41.0
35	36.99021905211569	40.0	36.0	41.0	31.0	41.0
36	36.846421347345604	40.0	35.0	41.0	31.0	41.0
37	36.767391497509465	39.0	35.0	41.0	31.0	41.0
38	36.65252198274135	39.0	35.0	41.0	30.0	41.0
39	36.555522646535636	39.0	35.0	41.0	30.0	41.0
40	36.45649359813092	39.0	35.0	41.0	30.0	41.0
41	36.35082312234199	39.0	35.0	41.0	30.0	41.0
42	36.23962973868109	39.0	35.0	41.0	29.0	41.0
43	36.153346934437685	39.0	35.0	40.0	29.0	41.0
44	36.01870645483324	39.0	35.0	40.0	29.0	41.0
45	35.92748787834968	39.0	35.0	40.0	28.0	41.0
46	35.804389753524994	39.0	35.0	40.0	28.0	41.0
47	35.75911933139712	39.0	35.0	40.0	28.0	41.0
48	35.64058065449072	38.0	35.0	40.0	27.0	41.0
49	35.52526338346926	38.0	35.0	40.0	27.0	41.0
50	35.39140517062649	38.0	34.0	40.0	26.0	41.0
51	33.62348403147883	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	17.0
9	33.0
10	34.0
11	24.0
12	12.0
13	12.0
14	24.0
15	43.0
16	62.0
17	93.0
18	197.0
19	333.0
20	620.0
21	1019.0
22	1573.0
23	2443.0
24	3499.0
25	4778.0
26	5961.0
27	6483.0
28	7351.0
29	8439.0
30	10725.0
31	13665.0
32	18084.0
33	26606.0
34	37146.0
35	48603.0
36	65998.0
37	113939.0
38	117962.0
39	78188.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.017702923308235	20.36506943706411	23.538563660659996	13.078663978967652
2	32.95033041623909	24.45585419523218	25.658175558780638	16.93563982974809
3	33.02559528061425	23.984054999102746	26.783489815723037	16.20685990455997
4	27.309995417902933	26.83122725284987	27.507217238441527	18.351560090805666
5	22.540433086573756	37.528594550614756	23.581771267986472	16.349201094825016
6	26.363783662297703	35.88740236910098	24.78374418308875	12.96506978551256
7	49.35685128046093	43.26754045922021	4.948839056889436	2.42676920342943
8	86.06119103163388	4.737330850057407	6.717563369705544	2.483914748603157
9	80.46859347042457	7.10991632010565	8.343946492256604	4.077543717213179
10	39.06455530138177	29.29928759715178	17.87070123507552	13.765455866390926
11	31.690515059070723	28.190001968733718	23.237504203159382	16.881978769036174
12	27.620462983450437	25.855745827765418	27.93424080923667	18.58955037954747
13	24.4164795208137	26.2801560352142	29.99252578082941	19.31083866314269
14	21.303092654184084	28.26352459087797	29.932244199640053	20.5011385552979
15	21.734123382110308	27.246752024921033	32.29577001008758	18.723354582881075
16	24.468049890848526	25.729607490247798	30.959818667428607	18.84252395147507
17	25.3203547902079	25.655039522765012	28.155854021007958	20.868751666019133
18	25.123307193892398	25.81201554776967	29.44650706566337	19.61817019267457
19	25.033059046331445	27.67569206217017	27.222186409465255	20.069062482033125
20	25.49301099529072	27.470978600038677	28.24523104745345	18.79077935721715
21	25.068426563618846	27.288391614239693	29.133426136769497	18.50975568537196
22	24.830958947546314	25.14682746400963	29.468459317772787	20.553754270671266
23	22.87337557689996	27.068172196253133	29.763072479019048	20.29537974782786
24	21.957827284558682	27.233685208189236	30.608931082124073	20.199556425128012
25	22.841840992520552	27.55460622712218	29.068788950002876	20.53476383035439
26	22.57092232561462	28.571553017302207	28.10219296029604	20.75533169678713
27	22.34547618093534	28.28652218832593	29.245452312216774	20.12254931852195
28	20.983042756366586	27.849393612591534	30.731410710956787	20.43615292008509
29	21.853989647596663	27.169222245645702	29.90767858418427	21.069109522573363
30	22.492521425223835	27.648687307591125	29.46689129976497	20.39189996742007
31	23.917675570105214	26.773559035006873	28.194357574310985	21.114407820576925
32	23.551456253168706	27.97709996811697	28.592459924073083	19.87898385464125
33	23.309110358849633	27.791899618971623	28.284431497648843	20.6145585245299
34	21.986922729814818	27.758622792361315	29.810984140368973	20.443470337454897
35	22.584337590792597	27.812806525742502	28.911812924998216	20.69104295846669
36	23.648150696984004	28.48025952440272	27.60600237293392	20.265587405679362
37	22.112364170440074	29.41479825706087	28.282166582748662	20.19067098975039
38	22.532418772311587	29.037951262515833	27.589625295963398	20.84000466920918
39	22.02037378064822	28.629743907814483	28.122751418620734	21.227130892916566
40	23.1758288281853	27.589799520186492	28.51440747212848	20.719964179499733
41	21.520524484601193	28.17310221909393	28.503954018743045	21.802419277561835
42	22.214111116725004	27.382124246262457	28.642984948769367	21.760779688243176
43	22.269688643890913	27.557045366245454	28.644378742554093	21.52888724730954
44	21.791268927284037	28.117350467704927	28.370498263855616	21.72088234115542
45	21.7342976063334	28.878710322610996	27.663670590776917	21.723321480278692
46	22.089889245661382	28.750655518639377	28.20028119789607	20.959174037803173
47	21.783254613021867	28.01978490277417	29.028543154468938	21.16841732973502
48	21.939707965357254	28.339660576368576	28.543851365830797	21.176780092443373
49	22.083094500960847	27.599033404010292	28.834631594169064	21.483240500859797
50	21.480278689067255	28.8618105729712	28.524512477067738	21.133398260893806
51	21.017364928315445	28.429734499706434	28.045395863568494	22.507504708409627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	350.0
1	809.0
2	1268.0
3	3222.0
4	5176.0
5	3643.5
6	2111.0
7	1883.5
8	1656.0
9	1634.0
10	1612.0
11	1607.5
12	1603.0
13	1588.0
14	1573.0
15	1598.0
16	1623.0
17	1625.5
18	1628.0
19	1718.5
20	1809.0
21	2194.5
22	2580.0
23	2882.5
24	3185.0
25	4323.5
26	6647.0
27	7832.0
28	9017.5
29	10203.0
30	11661.0
31	13119.0
32	14987.5
33	16856.0
34	19105.0
35	21354.0
36	23008.5
37	24663.0
38	26504.5
39	28346.0
40	31404.5
41	34463.0
42	36477.5
43	38492.0
44	39919.0
45	41346.0
46	42588.5
47	43831.0
48	42774.5
49	41718.0
50	40717.5
51	39717.0
52	37920.5
53	36124.0
54	33836.0
55	31548.0
56	29384.5
57	27221.0
58	25644.5
59	24068.0
60	21559.0
61	19050.0
62	16900.0
63	14750.0
64	12540.5
65	10331.0
66	8628.0
67	6925.0
68	5560.5
69	4196.0
70	3365.5
71	2535.0
72	2029.0
73	1523.0
74	1242.5
75	725.5
76	489.0
77	393.0
78	297.0
79	241.5
80	186.0
81	134.5
82	83.0
83	69.5
84	56.0
85	42.5
86	29.0
87	20.5
88	12.0
89	9.0
90	6.0
91	4.0
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	573973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.57471219869321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.34430910558504	23.97559529942824
2	8.16967888990416	5.6492859274835165
3	4.113856359756853	4.267061989960707
4	2.8433888950357615	3.9323741085928705
5	2.050248457464244	3.544337517632048
6	1.709864641177462	3.5470846720459552
7	1.4260843081690822	3.4514518168212973
8	1.284582200326753	3.5531247897489244
9	1.0722279698221004	3.3364776121189412
>10	7.954497562166073	43.30291293249158
>50	0.02225402787932844	0.5080047588765499
>100	0.007947867099743778	0.4698998220397471
>500	5.298578066495851E-4	0.17128901895016124
>1k	5.298578066495851E-4	0.29109973380941845
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1589	0.2768422904910161	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	935	0.16289964858974204	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010627677608528624	0.0
2	0.0	0.0	0.0	0.06672787744371252	0.0
3	0.0	0.0	0.0	0.08676366309913533	0.0
4	0.0	0.0	0.0	0.12126005927108069	0.0
5	0.0	0.0	0.0	0.2383387371879862	0.0
6	0.0	0.0	0.0	0.30855109909351136	0.0
7	0.0	0.0	0.0	0.3810283758992148	0.0
8	0.0	0.0	0.0	0.5491547511816758	0.0
9	0.0	0.0	0.0	0.6136177137252101	0.0
10	0.0	0.0	0.0	0.8517822266901056	0.0
11	0.0	0.0	0.0	1.0699109539995784	0.0
12	0.0	0.0	0.0	1.3089465880799271	0.0
13	0.0	0.0	0.0	1.3809011922163585	0.0
14	0.0	0.0	0.0	1.4176625032884822	0.0
15	0.0	0.0	0.0	1.4927531434405452	0.0
16	0.0	0.0	0.0	1.6340489883670486	0.0
17	0.0	0.0	0.0	1.794857946279703	0.0
18	0.0	0.0	0.0	1.9750057929554177	0.0
19	0.0	0.0	0.0	2.088774210633601	0.0
20	0.0	0.0	0.0	2.2107311667970446	0.0
21	0.0	0.0	0.0	2.3623062408858955	0.0
22	0.0	0.0	0.0	2.5283419254912687	0.0
23	0.0	0.0	0.0	2.711625808182615	0.0
24	0.0	0.0	0.0	2.8576257071325655	0.0
25	0.0	0.0	0.0	2.9919525831354434	0.0
26	0.0	0.0	0.0	3.123317647345781	0.0
27	0.0	0.0	0.0	3.2633939227106503	0.0
28	0.0	0.0	0.0	3.3937136415824436	0.0
29	0.0	0.0	0.0	3.546508285232929	0.0
30	0.0	0.0	0.0	3.723171647446831	0.0
31	0.0	0.0	0.0	3.893737161852561	0.0
32	0.0	0.0	0.0	4.060992416019569	0.0
33	0.0	0.0	0.0	4.229467239748211	0.0
34	0.0	0.0	0.0	4.406130601962113	0.0
35	0.0	0.0	0.0	4.583316636845288	0.0
36	0.0	0.0	0.0	4.764335604636455	0.0
37	0.0	0.0	0.0	4.957027595374695	0.0
38	0.0	0.0	0.0	5.152681397905476	0.0
39	0.0	0.0	0.0	5.416979544333967	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	20	7.0303306E-4	45.000004	1
ATCCGGT	20	7.0303306E-4	45.000004	25
CGGATAG	20	7.0303306E-4	45.000004	2
TTGCGAA	30	2.1635951E-6	45.000004	1
CGGCTAG	35	1.210501E-7	45.000004	2
CCTATCA	20	7.0303306E-4	45.000004	16
GTGCGAA	30	2.1635951E-6	45.000004	1
CGTTATT	190	0.0	42.63158	1
CGTTTTA	350	0.0	39.857143	1
TCACGAC	40	3.454188E-7	39.375004	25
CGTCTCG	40	3.454188E-7	39.375004	42
TCGTATA	35	6.2428226E-6	38.57143	32
ACATACG	70	0.0	38.57143	17
CGTAAAG	65	9.094947E-12	38.07692	2
TTGGACG	30	1.1392097E-4	37.500004	1
CGTTAAG	30	1.1392097E-4	37.500004	2
CGCTAAG	30	1.1392097E-4	37.500004	2
CGTAACG	30	1.1392097E-4	37.500004	34
TATACGG	30	1.1392097E-4	37.500004	2
CCCGGAT	30	1.1392097E-4	37.500004	19
>>END_MODULE