##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934602.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544082
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.817426417341505	31.0	31.0	34.0	30.0	34.0
2	31.996796438772098	33.0	31.0	34.0	30.0	34.0
3	31.35696273723446	31.0	31.0	34.0	28.0	34.0
4	35.4124856179767	37.0	35.0	37.0	33.0	37.0
5	31.815970754408344	37.0	35.0	37.0	0.0	37.0
6	33.82065387202665	37.0	35.0	37.0	17.0	37.0
7	21.11419050804842	32.0	0.0	37.0	0.0	37.0
8	28.32164636948107	35.0	17.0	37.0	17.0	37.0
9	34.989545693480025	37.0	32.0	39.0	32.0	39.0
10	36.34395918262321	37.0	35.0	39.0	32.0	39.0
11	36.76288684426245	39.0	37.0	39.0	33.0	39.0
12	37.1916769898655	39.0	37.0	39.0	34.0	39.0
13	37.05903521895597	39.0	37.0	39.0	33.0	39.0
14	38.51756904290162	40.0	38.0	41.0	34.0	41.0
15	38.667776180796274	40.0	38.0	41.0	35.0	41.0
16	38.70107079447583	40.0	38.0	41.0	35.0	41.0
17	38.653407023206064	40.0	38.0	41.0	35.0	41.0
18	38.48943909190159	40.0	38.0	41.0	34.0	41.0
19	38.331505177528385	40.0	37.0	41.0	34.0	41.0
20	38.202978595138234	40.0	37.0	41.0	34.0	41.0
21	38.12145228108998	40.0	37.0	41.0	34.0	41.0
22	38.040262313401286	40.0	36.0	41.0	34.0	41.0
23	37.936612495910545	40.0	36.0	41.0	34.0	41.0
24	37.77454317547723	39.0	35.0	41.0	33.0	41.0
25	37.63874195433777	39.0	35.0	41.0	33.0	41.0
26	37.515762329942916	39.0	35.0	41.0	33.0	41.0
27	37.47348929021728	39.0	35.0	41.0	33.0	41.0
28	37.39352340272239	39.0	35.0	41.0	33.0	41.0
29	37.362761495509865	39.0	35.0	41.0	33.0	41.0
30	37.19439533011568	39.0	35.0	41.0	33.0	41.0
31	37.00101822886991	39.0	35.0	41.0	32.0	41.0
32	36.77300480442286	39.0	35.0	41.0	31.0	41.0
33	36.40975073610228	39.0	35.0	41.0	30.0	41.0
34	36.03075455537952	39.0	35.0	41.0	27.0	41.0
35	35.684258990372776	39.0	35.0	41.0	25.0	41.0
36	35.40571090387111	39.0	35.0	41.0	23.0	41.0
37	35.28670494521047	39.0	35.0	41.0	23.0	41.0
38	35.131509588628184	39.0	35.0	41.0	21.0	41.0
39	35.048518421855526	39.0	35.0	41.0	21.0	41.0
40	34.96247808234788	39.0	35.0	41.0	20.0	41.0
41	34.892892983043	39.0	35.0	40.0	20.0	41.0
42	34.771071272344976	39.0	35.0	40.0	19.0	41.0
43	34.6830918868847	39.0	34.0	40.0	18.0	41.0
44	34.539508750519225	39.0	34.0	40.0	18.0	41.0
45	34.472623611882035	38.0	34.0	40.0	18.0	41.0
46	34.342407945861105	38.0	34.0	40.0	18.0	41.0
47	34.3156123525498	38.0	34.0	40.0	18.0	41.0
48	34.203671505398084	38.0	34.0	40.0	18.0	41.0
49	34.09047900867884	38.0	33.0	40.0	16.0	41.0
50	33.931001209376525	38.0	33.0	40.0	15.0	41.0
51	32.27657411934231	36.0	30.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	28.0
10	17.0
11	11.0
12	16.0
13	6.0
14	11.0
15	21.0
16	40.0
17	61.0
18	200.0
19	321.0
20	732.0
21	1284.0
22	2466.0
23	4242.0
24	7031.0
25	10262.0
26	11921.0
27	11779.0
28	10717.0
29	10147.0
30	10770.0
31	13044.0
32	16470.0
33	23133.0
34	32185.0
35	43288.0
36	56472.0
37	98694.0
38	106998.0
39	71698.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.030965920578154	19.766873375704396	23.464661576747623	20.737499126969833
2	38.73037520079694	23.00351785208847	23.032925184071516	15.233181763043072
3	28.296286221562188	22.461871556125732	34.257887597825324	14.983954624486751
4	24.799938244602835	23.597545958146014	34.924698850540906	16.677816946710237
5	20.60773927459464	35.009612521641955	29.458427222367217	14.924220981396186
6	23.324792954003257	31.801640193941356	32.29568337125654	12.577883480798851
7	45.22719001915153	41.51451435629188	11.056421642325972	2.20187398223062
8	78.99452656033465	3.806595329380498	15.191276314967228	2.0076017953176173
9	73.3819534555453	6.720494337250635	16.351579357523313	3.5459728496807466
10	32.454850555614776	32.46808385500715	23.862763333468116	11.214302255909955
11	23.92782705548061	25.375954359820764	34.949327491076716	15.746891093621917
12	22.25859337379292	23.421469557897524	36.65881245841619	17.66112460989336
13	20.410526354483334	24.75693002157763	37.9942729220963	16.838270701842735
14	17.87340878764598	27.39035660065946	36.97089776908628	17.765336842608285
15	17.457846427560554	26.371208751622	39.62270393065751	16.548240890159942
16	20.641557706375142	25.617278277906642	37.78860539403987	15.952558621678351
17	20.543043144231934	24.821258560290545	36.41675335703074	18.21894493844678
18	20.98029341165486	24.827691414161837	37.280961325682526	16.911053848500774
19	20.406115254685876	26.19072125157605	35.56265415874813	17.840509334989946
20	21.43684224069166	26.071989148694495	36.35279240996761	16.138376200646228
21	20.75753287188328	26.103602030576273	37.451707647009094	15.687157450531352
22	21.385195613896435	23.387651126117017	36.68619803632541	18.54095522366114
23	18.30312342624825	26.218290625310154	36.98982873905036	18.48875720939123
24	18.29356604335376	24.962229957984274	38.6790961656515	18.065107833010465
25	18.647556802099682	27.65153046783389	36.31254112431582	17.388371605750606
26	19.06844924110704	27.98015740274444	35.32518995298503	17.626203403163494
27	18.614105961968967	27.65630915928114	36.23847140688352	17.491113471866374
28	17.334519429056648	27.16796365253767	37.473579350171484	18.0239375682342
29	18.52459739524557	25.91484371841009	37.40925081145857	18.151308074885772
30	19.3318286581802	25.89021507787429	37.135027440716655	17.64292882322885
31	19.962248337566763	26.87646347425572	35.512110306902265	17.64917788127525
32	19.90288228612599	27.286879551244112	35.73174631765065	17.078491844979247
33	19.23809278748424	26.844483000724157	35.299090945850075	18.61833326594153
34	19.020294734984798	26.863597766513138	36.03758257027433	18.07852492822773
35	19.204825743178418	27.06926529456957	34.99564403895001	18.730264923302002
36	20.197690789256033	28.074077069265297	33.815307251480476	17.912924889998198
37	19.762094684257153	28.30639499193136	33.9009561058811	18.030554217930387
38	19.098224164739875	29.01842001757088	32.98436632713451	18.898989490554733
39	19.962983520866338	28.37568601791642	32.81362000580795	18.847710455409295
40	20.146411754110595	27.77283571226396	33.01009774261968	19.07065479100577
41	19.375388268680087	28.095397384953003	32.36883410956437	20.160380236802542
42	20.183722306564082	27.711264110924454	32.78402887800001	19.320984704511453
43	20.398028238390538	27.581871850199054	32.39640348329848	19.623696428111938
44	19.776798350248676	28.716627273094865	32.133207862050206	19.373366514606253
45	19.62553438636088	28.039891045835002	31.85696273723446	20.477611830569657
46	20.026393080454785	28.491477387599662	31.977716594189847	19.504412937755706
47	19.02305167235821	28.320731066273098	33.068361019111094	19.5878562422576
48	19.877702258115505	27.777614403711205	32.69084439477873	19.65383894339456
49	20.154314974581037	26.816362239515367	33.287078050735	19.742244735168597
50	19.01349428946372	27.936046404769872	32.95330483272742	20.09715447303899
51	19.36215496928772	27.525997919431262	31.78748791542451	21.324359195856506
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	440.0
1	748.0
2	1056.0
3	10919.0
4	20782.0
5	14500.0
6	8218.0
7	7144.5
8	6071.0
9	5857.5
10	5644.0
11	5519.5
12	5395.0
13	5237.5
14	5080.0
15	4913.5
16	4747.0
17	4424.0
18	4101.0
19	4024.5
20	3948.0
21	4097.5
22	4247.0
23	4606.5
24	4966.0
25	5604.5
26	7361.5
27	8480.0
28	9877.5
29	11275.0
30	12588.5
31	13902.0
32	16031.0
33	18160.0
34	19963.5
35	21767.0
36	23010.5
37	24254.0
38	25977.5
39	27701.0
40	30406.5
41	33112.0
42	34988.0
43	36864.0
44	38324.0
45	39784.0
46	41802.5
47	43821.0
48	41760.0
49	39699.0
50	37537.5
51	35376.0
52	32038.0
53	28700.0
54	25719.0
55	22738.0
56	20578.5
57	18419.0
58	15835.0
59	13251.0
60	11405.5
61	9560.0
62	8156.0
63	6752.0
64	5392.0
65	4032.0
66	3143.5
67	2255.0
68	1797.0
69	1339.0
70	1052.0
71	765.0
72	624.0
73	483.0
74	369.5
75	202.0
76	148.0
77	127.0
78	106.0
79	84.5
80	63.0
81	46.0
82	29.0
83	23.0
84	17.0
85	19.0
86	21.0
87	12.5
88	4.0
89	5.0
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	544082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.311780806199955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85418040311941	33.61233815256976
2	8.088085662520292	7.167949580387356
3	3.676789881480478	4.8877532189585065
4	2.321912271860334	4.115522705676036
5	1.677180594969398	3.7159429448348
6	1.2554524001607539	3.3378798941124583
7	1.015532601278354	3.150004062057755
8	0.9139771139897949	3.2399962829599214
9	0.7781877250613699	3.1034595509095344
>10	4.376382236870708	28.806043997592027
>50	0.02684760658811318	0.8275230881396133
>100	0.012741237024303147	1.0293629056560651
>500	0.0013651325383181943	0.40186489147356275
>1k	9.100883588787961E-4	1.1412720952033943
>5k	4.5504417943939806E-4	1.463086629469228
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7256	1.3336225054311666	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4044	0.7432703158715047	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1616	0.2970140530287714	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	828	0.15218294301226654	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG	588	0.10807194503769653	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC	577	0.10605019096386206	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0277531695590003	0.0
2	0.0	0.0	0.0	0.13031123984987558	0.0
3	0.0	0.0	0.0	0.21412213600155858	0.0
4	0.0	0.0	0.0	0.2983006238030297	0.0
5	0.0	0.0	0.0	0.5986230016798938	0.0
6	0.0	0.0	0.0	0.8239566829999889	0.0
7	0.0	0.0	0.0	1.0430413062736867	0.0
8	0.0	0.0	0.0	1.41063295606177	0.0
9	0.0	0.0	0.0	1.5698001404200101	0.0
10	0.0	0.0	0.0	2.0868177958469496	0.0
11	0.0	0.0	0.0	2.4911686106138413	0.0
12	0.0	0.0	0.0	2.964442859715999	0.0
13	0.0	0.0	0.0	3.1305942854202122	0.0
14	0.0	0.0	0.0	3.220654239618293	0.0
15	0.0	0.0	0.0	3.3432460548226186	0.0
16	0.0	0.0	0.0	3.5693149194422897	0.0
17	0.0	0.0	0.0	3.864858605871909	0.0
18	0.0	0.0	0.0	4.166099962873243	0.0
19	0.0	0.0	0.0	4.363129087159656	0.0
20	0.0	0.0	0.0	4.555930907473506	0.0
21	0.0	0.0	0.0	4.807914983403237	0.0
22	0.0	0.0	0.0	5.084343904043876	0.0
23	0.0	0.0	0.0	5.359486253910256	0.0
24	0.0	0.0	0.0	5.583533364456093	0.0
25	0.0	0.0	0.0	5.787914321738268	0.0
26	0.0	0.0	0.0	5.995236012218746	0.0
27	0.0	0.0	0.0	6.1941030947541	0.0
28	0.0	0.0	0.0	6.419069184424407	0.0
29	0.0	0.0	0.0	6.652673677864733	0.0
30	0.0	0.0	0.0	6.930756760929419	0.0
31	0.0	0.0	0.0	7.194319973827475	0.0
32	0.0	0.0	0.0	7.437114258512504	0.0
33	0.0	0.0	0.0	7.6747622601005	0.0
34	0.0	0.0	0.0	7.917556544785529	0.0
35	0.0	0.0	0.0	8.174686903812294	0.0
36	0.0	0.0	0.0	8.43512558768715	0.0
37	0.0	0.0	0.0	8.697586025635841	0.0
38	0.0	0.0	0.0	8.956370547086653	0.0
39	0.0	0.0	0.0	9.260185045636502	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	20	7.0300093E-4	45.0	1
CGAACGG	20	7.0300093E-4	45.0	2
ACGCATA	20	7.0300093E-4	45.0	1
CGCATAG	45	3.8380676E-10	45.0	2
ATAGCGG	25	3.8880884E-5	45.0	2
TCGATAG	20	7.0300093E-4	45.0	2
ATCGTTC	20	7.0300093E-4	45.0	33
ACGATAT	20	7.0300093E-4	45.0	15
CGAATGG	40	6.8030204E-9	45.0	2
GGTGCGC	20	7.0300093E-4	45.0	8
CGGTCTA	20	7.0300093E-4	45.0	31
AACTCGC	20	7.0300093E-4	45.0	24
AGGATCG	25	3.8880884E-5	45.0	7
TACGTTG	20	7.0300093E-4	45.0	1
CGGACGG	30	2.163386E-6	44.999996	2
CGTTTTA	1700	0.0	44.073532	1
CGTTATT	770	0.0	43.538963	1
CGTTTTT	3185	0.0	43.09262	1
CGGTTTT	380	0.0	42.63158	1
AGGCGAT	245	0.0	42.2449	7
>>END_MODULE