##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934601.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421346
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.043622106297434	33.0	31.0	34.0	30.0	34.0
2	32.24190332885562	33.0	31.0	34.0	30.0	34.0
3	32.132551394815664	33.0	31.0	34.0	30.0	34.0
4	35.88185244430943	37.0	35.0	37.0	35.0	37.0
5	32.1017595989994	37.0	35.0	37.0	0.0	37.0
6	33.99859023225567	37.0	35.0	37.0	19.0	37.0
7	20.724976622538247	32.0	0.0	37.0	0.0	37.0
8	28.102400402519546	35.0	17.0	37.0	17.0	37.0
9	34.897395489692556	37.0	32.0	39.0	32.0	39.0
10	36.33715521210596	37.0	35.0	39.0	32.0	39.0
11	36.7776150716988	39.0	37.0	39.0	33.0	39.0
12	37.10089807426675	39.0	37.0	39.0	34.0	39.0
13	36.87559630327569	39.0	37.0	39.0	33.0	39.0
14	38.266916026258706	40.0	38.0	41.0	34.0	41.0
15	38.46859825416641	40.0	38.0	41.0	34.0	41.0
16	38.53272132641582	40.0	38.0	41.0	34.0	41.0
17	38.49580392361622	40.0	38.0	41.0	34.0	41.0
18	38.411664048074506	40.0	38.0	41.0	34.0	41.0
19	38.35950501488088	40.0	37.0	41.0	34.0	41.0
20	38.35696078757126	40.0	37.0	41.0	34.0	41.0
21	38.26682821244298	40.0	37.0	41.0	34.0	41.0
22	38.23881797857343	40.0	37.0	41.0	34.0	41.0
23	38.175651364911495	40.0	37.0	41.0	34.0	41.0
24	38.076061004495116	40.0	37.0	41.0	34.0	41.0
25	37.95075543614986	40.0	36.0	41.0	33.0	41.0
26	37.808672682308604	40.0	36.0	41.0	33.0	41.0
27	37.76975217517195	40.0	36.0	41.0	33.0	41.0
28	37.69332804868208	40.0	36.0	41.0	33.0	41.0
29	37.68519221732258	40.0	36.0	41.0	33.0	41.0
30	37.55129988180735	40.0	36.0	41.0	33.0	41.0
31	37.428403260028574	40.0	36.0	41.0	33.0	41.0
32	37.335724084244305	40.0	36.0	41.0	32.0	41.0
33	37.21048259625106	40.0	36.0	41.0	32.0	41.0
34	37.068622936968666	40.0	35.0	41.0	31.0	41.0
35	36.94032932554243	40.0	35.0	41.0	31.0	41.0
36	36.80019508907169	40.0	35.0	41.0	31.0	41.0
37	36.742952347951565	39.0	35.0	41.0	31.0	41.0
38	36.64444660682669	39.0	35.0	41.0	30.0	41.0
39	36.59096561970447	39.0	35.0	41.0	30.0	41.0
40	36.471467629928846	39.0	35.0	41.0	30.0	41.0
41	36.35117456911897	39.0	35.0	41.0	30.0	41.0
42	36.27912926668344	39.0	35.0	41.0	30.0	41.0
43	36.17882215566304	39.0	35.0	40.0	29.0	41.0
44	36.066766980106614	39.0	35.0	40.0	29.0	41.0
45	35.98235891642498	39.0	35.0	40.0	29.0	41.0
46	35.82835959045535	39.0	35.0	40.0	28.0	41.0
47	35.79946884508219	39.0	35.0	40.0	28.0	41.0
48	35.684271358930665	38.0	35.0	40.0	28.0	41.0
49	35.58986438698837	38.0	35.0	40.0	27.0	41.0
50	35.43746469647273	38.0	34.0	40.0	27.0	41.0
51	33.63700853930024	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	19.0
10	19.0
11	19.0
12	12.0
13	10.0
14	15.0
15	20.0
16	33.0
17	66.0
18	128.0
19	226.0
20	406.0
21	701.0
22	1151.0
23	1829.0
24	2680.0
25	3470.0
26	4271.0
27	4667.0
28	5349.0
29	6273.0
30	7707.0
31	9836.0
32	13355.0
33	19721.0
34	27843.0
35	35789.0
36	46762.0
37	81778.0
38	88471.0
39	58702.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.6717994237515	20.308962230565854	26.402054368618664	12.61718397706398
2	31.987962387206714	24.834696425265694	26.414158435110334	16.76318275241725
3	32.44862891780152	24.04769476866993	27.544108642303478	15.959567671225075
4	28.3322969720847	26.19628523826024	27.136130401143006	18.335287388512054
5	22.72455416688422	37.872437379256	22.81094397478557	16.592064479074207
6	26.836851423770486	35.88428512433961	23.621916429727587	13.656947022162308
7	49.6803102438376	43.339440744661154	4.558249039981392	2.4219999715198433
8	86.54478741936555	4.737911360259739	6.419664598690862	2.2976366216838415
9	79.95566588979128	8.145562079621024	8.195639688047352	3.703132342540335
10	38.948749958466436	35.61704632297447	14.176947212030019	11.257256506529076
11	32.95889838754847	27.112634272070935	24.61207653567377	15.31639080470682
12	31.803078704912352	24.43811024668562	26.14573296055973	17.613078087842297
13	25.814413807179847	25.520593526460438	28.14480260878233	20.520190057577384
14	20.2002629667779	29.43922571947995	29.34405453000622	21.016456783735933
15	20.271226023268287	28.214578992087265	32.75526526892388	18.75892971572057
16	24.202911621327843	26.02920165374775	31.896351217289354	17.871535507635055
17	23.958219610486395	25.06135100368818	28.53426874824966	22.44616063757577
18	24.17182078386884	26.638202332524813	28.313547535754463	20.876429347851886
19	25.709986566859538	27.69481613685664	26.882419674092077	19.712777622191737
20	28.019964589672146	27.002985669734613	26.961452108243584	18.01559763234966
21	26.635116982242625	26.0674125303195	27.801379388910775	19.496091098527103
22	25.19164771945147	25.754605478632765	27.64972255580924	21.404024246106527
23	24.558201573053974	27.21373882747196	28.158568017733643	20.069491581740422
24	23.87966184560907	25.87683281673494	29.672763002378094	20.570742335277895
25	23.55570006597903	27.19498939114172	27.658266602744536	21.59104394013471
26	22.978502228572243	30.004556825032157	26.955756076953385	20.061184869442215
27	22.389437659310875	29.246747328798662	28.36718516373717	19.9966298481533
28	21.180692352603323	30.462375339981868	28.844939788202566	19.51199251921224
29	22.336512035239448	27.658978606655815	29.436377703834854	20.568131654269887
30	23.337826869128932	27.00227366582334	28.98401788553825	20.67588157950948
31	24.036540040726624	28.046308734389314	26.69112795659624	21.22602326828782
32	24.850597845950833	29.324118420490525	26.827358038286825	18.99792569527182
33	24.703450370954037	28.770891381429987	26.621588907928402	19.90406933968757
34	22.742354264666094	29.296350267950803	28.139818581403407	19.821476885979695
35	23.6667726761379	27.185021336383876	27.45795616903922	21.690249818439003
36	22.605174844427147	30.171640409544647	26.527367057003033	20.695817689025173
37	23.874915152867242	30.12559748994888	27.084391450257034	18.915095906926847
38	23.131108400222146	28.78133410546202	26.612807526356015	21.474749967959823
39	23.312669397597226	28.149549301524164	27.02339644852449	21.51438485235412
40	23.658703298476784	27.46792422379707	28.784182121107122	20.089190356619024
41	20.48126717709436	28.446217597888673	28.91875086033806	22.15376436467891
42	22.865056272042455	28.433638862122816	26.788909827077983	21.912395038756745
43	23.18759404384995	28.060786147251903	27.110023591062927	21.641596217835225
44	21.908123015289096	29.983196707693914	27.444665429362093	20.664014847654897
45	22.844882827889666	28.81954498203377	25.689101118795477	22.646471071281084
46	21.54856104009531	30.326145258291287	26.085687297375554	22.039606404237848
47	22.84512016252676	29.127605340978675	27.375363715331343	20.651910781163224
48	22.276941041329454	27.546956657948574	28.528335382322368	21.647766918399604
49	21.599113317795826	27.909366648787458	28.27130197035216	22.22021806306456
50	20.210231021535748	29.905350946727864	27.908654644876187	21.975763386860205
51	20.87144532047296	28.41939878389732	27.63927983177721	23.069876063852508
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	427.0
1	803.0
2	1179.0
3	2415.0
4	3651.0
5	2560.0
6	1469.0
7	1387.5
8	1306.0
9	1326.5
10	1347.0
11	1342.5
12	1338.0
13	1375.0
14	1412.0
15	1360.5
16	1309.0
17	1383.5
18	1458.0
19	1584.5
20	1711.0
21	1823.5
22	1936.0
23	2113.5
24	2291.0
25	2835.5
26	4294.0
27	5208.0
28	6195.0
29	7182.0
30	8331.5
31	9481.0
32	10870.5
33	12260.0
34	13778.0
35	15296.0
36	16962.5
37	18629.0
38	19546.0
39	20463.0
40	21949.0
41	23435.0
42	25391.0
43	27347.0
44	28418.0
45	29489.0
46	29450.5
47	29412.0
48	29010.0
49	28608.0
50	28179.0
51	27750.0
52	26300.5
53	24851.0
54	23145.5
55	21440.0
56	21031.5
57	20623.0
58	20616.5
59	20610.0
60	19786.0
61	18962.0
62	16274.0
63	13586.0
64	11114.0
65	8642.0
66	7021.0
67	5400.0
68	4467.0
69	3534.0
70	2762.0
71	1990.0
72	1590.5
73	1191.0
74	943.0
75	598.0
76	501.0
77	362.0
78	223.0
79	189.0
80	155.0
81	113.5
82	72.0
83	59.0
84	46.0
85	33.5
86	21.0
87	16.5
88	12.0
89	9.0
90	6.0
91	4.0
92	2.0
93	2.5
94	3.0
95	4.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	421346.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.64647409139485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.65530016474851	22.087070039740038
2	7.840138538351249	5.119057593304607
3	4.3264486063892935	4.237298770087183
4	3.1395434200367194	4.0998009168415175
5	2.4804559004804076	4.048906964494056
6	2.004013683713559	3.9254388482493283
7	1.6258133532761274	3.715395146062101
8	1.3428687738943905	3.5071944508069115
9	1.168125943142685	3.4321674004464455
>10	8.368718012203326	44.032881382228126
>50	0.0316464388157596	0.688602919124749
>100	0.015455237561184922	0.7210388556501647
>500	7.359636933897582E-4	0.14464025028370514
>1k	7.359636933897582E-4	0.24050646268104459
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1001	0.23757197172869804	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	602	0.14287545152914707	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.7466927418321286E-4	0.0	0.0	0.018986770967328512	0.0
2	4.7466927418321286E-4	0.0	0.0	0.0738110721354896	0.0
3	4.7466927418321286E-4	0.0	0.0	0.09778187048174185	0.0
4	4.7466927418321286E-4	0.0	0.0	0.12104066491671928	0.0
5	4.7466927418321286E-4	0.0	0.0	0.19413973314093405	0.0
6	4.7466927418321286E-4	0.0	0.0	0.2558467387847517	0.0
7	4.7466927418321286E-4	0.0	0.0	0.309959036041638	0.0
8	4.7466927418321286E-4	0.0	0.0	0.4886720177716176	0.0
9	4.7466927418321286E-4	0.0	0.0	0.5468190038590612	0.0
10	4.7466927418321286E-4	0.0	0.0	0.7195986196617506	0.0
11	4.7466927418321286E-4	0.0	0.0	0.9645279651402885	0.0
12	4.7466927418321286E-4	0.0	0.0	1.1721957725954442	0.0
13	4.7466927418321286E-4	0.0	0.0	1.2611962615047965	0.0
14	4.7466927418321286E-4	0.0	0.0	1.2970337917056292	0.0
15	4.7466927418321286E-4	0.0	0.0	1.3722688716636684	0.0
16	4.7466927418321286E-4	0.0	0.0	1.5270110550473956	0.0
17	4.7466927418321286E-4	0.0	0.0	1.7052493675031921	0.0
18	4.7466927418321286E-4	0.0	0.0	1.898914431369943	0.0
19	4.7466927418321286E-4	0.0	0.0	2.019005757738296	0.0
20	4.7466927418321286E-4	0.0	0.0	2.1502518120499543	0.0
21	4.7466927418321286E-4	0.0	0.0	2.3299141323283004	0.0
22	4.7466927418321286E-4	0.0	0.0	2.5209685151870436	0.0
23	4.7466927418321286E-4	0.0	0.0	2.7167695907876186	0.0
24	4.7466927418321286E-4	0.0	0.0	2.884327844574293	0.0
25	4.7466927418321286E-4	0.0	0.0	3.0359846776758292	0.0
26	4.7466927418321286E-4	0.0	0.0	3.175300109648602	0.0
27	4.7466927418321286E-4	0.0	0.0	3.305834160048986	0.0
28	4.7466927418321286E-4	0.0	0.0	3.451794961860324	0.0
29	4.7466927418321286E-4	0.0	0.0	3.6188785463728146	0.0
30	4.7466927418321286E-4	0.0	0.0	3.812306275602474	0.0
31	4.7466927418321286E-4	0.0	0.0	3.9850858914051637	0.0
32	4.7466927418321286E-4	0.0	0.0	4.164748211683509	0.0
33	4.7466927418321286E-4	0.0	0.0	4.334205142566916	0.0
34	4.7466927418321286E-4	0.0	0.0	4.51932615949837	0.0
35	4.7466927418321286E-4	0.0	0.0	4.717975250744044	0.0
36	4.7466927418321286E-4	0.0	0.0	4.926355062110474	0.0
37	4.7466927418321286E-4	0.0	0.0	5.1245294840819655	0.0
38	4.7466927418321286E-4	0.0	0.0	5.32460258315019	0.0
39	4.7466927418321286E-4	0.0	0.0	5.556003854314507	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	3.886585E-5	45.0	25
ACCGACC	20	7.028195E-4	45.0	32
TTCCGCA	20	7.028195E-4	45.0	14
CGCGAAG	20	7.028195E-4	45.0	2
TATGACG	20	7.028195E-4	45.0	1
GGCGCGA	20	7.028195E-4	45.0	9
CGGTCAA	20	7.028195E-4	45.0	44
CGCAAAA	20	7.028195E-4	45.0	45
CCGCAAA	20	7.028195E-4	45.0	44
AGGCGAT	255	0.0	43.235294	7
AAGGCGA	150	0.0	42.000004	6
CGTTTTA	270	0.0	41.666664	1
TACGAAT	55	6.002665E-11	40.909092	12
TTACGGG	105	0.0	40.714287	3
CTCGTAC	45	1.9233994E-8	40.0	29
TACGGGA	135	0.0	40.0	4
CCGATTA	35	6.2388717E-6	38.571426	11
TTAACGG	35	6.2388717E-6	38.571426	2
ATAGGAT	185	0.0	37.702705	5
CTCACGA	30	1.13869464E-4	37.500004	24
>>END_MODULE