##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934600.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	946277
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.960683816683698	33.0	31.0	34.0	30.0	34.0
2	32.15819152320093	33.0	31.0	34.0	30.0	34.0
3	31.91406427504843	33.0	31.0	34.0	30.0	34.0
4	35.73253920363699	37.0	35.0	37.0	35.0	37.0
5	32.04883665142448	37.0	35.0	37.0	0.0	37.0
6	33.94055546103308	37.0	35.0	37.0	19.0	37.0
7	20.882474159257807	32.0	0.0	37.0	0.0	37.0
8	28.166101469231524	35.0	17.0	37.0	17.0	37.0
9	34.86628651018676	37.0	32.0	39.0	32.0	39.0
10	36.21626859788413	37.0	35.0	39.0	32.0	39.0
11	36.699668278950035	38.0	35.0	39.0	33.0	39.0
12	37.05338817280775	39.0	37.0	39.0	34.0	39.0
13	36.878518657856	39.0	37.0	39.0	33.0	39.0
14	38.20877713396817	40.0	38.0	41.0	34.0	41.0
15	38.371468396674544	40.0	38.0	41.0	34.0	41.0
16	38.46129621664692	40.0	38.0	41.0	34.0	41.0
17	38.44056867069579	40.0	38.0	41.0	34.0	41.0
18	38.32142702401094	40.0	38.0	41.0	34.0	41.0
19	38.252023456133884	40.0	37.0	41.0	34.0	41.0
20	38.20353976689701	40.0	37.0	41.0	34.0	41.0
21	38.11326704548457	40.0	37.0	41.0	34.0	41.0
22	38.10510030361089	40.0	37.0	41.0	34.0	41.0
23	38.029345529902976	40.0	37.0	41.0	34.0	41.0
24	37.910115114284714	40.0	36.0	41.0	33.0	41.0
25	37.7657472389163	40.0	36.0	41.0	33.0	41.0
26	37.651898968272505	40.0	36.0	41.0	33.0	41.0
27	37.61715015793473	40.0	36.0	41.0	33.0	41.0
28	37.530196760567996	40.0	36.0	41.0	33.0	41.0
29	37.50185727857699	40.0	36.0	41.0	33.0	41.0
30	37.33585197568999	40.0	36.0	41.0	33.0	41.0
31	37.20772458804346	40.0	35.0	41.0	32.0	41.0
32	37.07349433622502	39.0	35.0	41.0	31.0	41.0
33	36.88741668665729	39.0	35.0	41.0	31.0	41.0
34	36.64464633505834	39.0	35.0	41.0	30.0	41.0
35	36.47935541073068	39.0	35.0	41.0	30.0	41.0
36	36.314929983503774	39.0	35.0	41.0	30.0	41.0
37	36.20740438581937	39.0	35.0	41.0	29.0	41.0
38	36.069740678469415	39.0	35.0	41.0	28.0	41.0
39	35.975265170769234	39.0	35.0	41.0	28.0	41.0
40	35.9024186364035	39.0	35.0	41.0	27.0	41.0
41	35.76845574815831	39.0	35.0	40.0	27.0	41.0
42	35.66199326412879	39.0	35.0	40.0	26.0	41.0
43	35.554293298896624	39.0	35.0	40.0	26.0	41.0
44	35.41235494469378	39.0	35.0	40.0	25.0	41.0
45	35.319462482972746	38.0	34.0	40.0	25.0	41.0
46	35.19687364270715	38.0	34.0	40.0	24.0	41.0
47	35.15775718949103	38.0	34.0	40.0	24.0	41.0
48	35.0258645195857	38.0	34.0	40.0	23.0	41.0
49	34.902241098536685	38.0	34.0	40.0	24.0	41.0
50	34.74139601829063	38.0	34.0	40.0	23.0	41.0
51	32.96854515115553	36.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	32.0
9	56.0
10	55.0
11	43.0
12	25.0
13	26.0
14	44.0
15	54.0
16	108.0
17	202.0
18	370.0
19	660.0
20	1166.0
21	2023.0
22	3288.0
23	5228.0
24	8014.0
25	10919.0
26	13278.0
27	13906.0
28	14535.0
29	16280.0
30	18751.0
31	23671.0
32	31087.0
33	44726.0
34	61531.0
35	78689.0
36	106485.0
37	180097.0
38	186818.0
39	124093.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54111322583134	20.102147679802005	24.28083954275545	14.07589955161121
2	35.21262801484132	22.98724369291444	25.186388340834657	16.613739951409574
3	31.880939724837443	22.872055434085368	28.94448454310947	16.30252029796772
4	27.58494605702136	25.15130347667755	28.730382329909744	18.533368136391353
5	22.25954979355939	37.0118897532118	24.048666510968776	16.679893942260033
6	26.09320526653401	34.047218731935786	26.820687811285705	13.038888190244505
7	48.3224256745118	42.73759163543021	6.3997117123210225	2.540270977736963
8	83.4055989947975	4.592524176324692	9.211150646163862	2.790726182713941
9	77.20234138629598	7.313926049137831	11.229481430912935	4.254251133653254
10	37.63232119136363	29.91122049886027	19.285367815132357	13.171090494643744
11	30.36087741750037	26.660058312735064	26.38455758726039	16.59450668250417
12	26.531132004687848	24.298487652135687	30.15343287430636	19.01694746887011
13	24.384720330304972	25.284985263300282	31.651937012101104	18.678357394293638
14	20.740121550032388	26.612820558884977	32.604829241332084	20.042228649750548
15	20.78577414435731	26.198671213608698	33.81853305110449	19.197021590929506
16	24.858788705632705	25.80407216914286	31.144157577538078	18.192981547686355
17	25.431771035331092	25.32080986856914	29.740234624745188	19.507184471354584
18	25.46537641726471	24.413464556361404	30.80852646740859	19.31263255896529
19	24.738422259021405	26.74882724614463	28.913098384511088	19.599652110322875
20	25.455442750906975	26.792471971737662	29.658123361341342	18.09396191601402
21	24.63908559544404	26.151750491663645	31.013963141870722	18.195200771021593
22	24.448232388613484	23.784156224868617	31.498916279271295	20.26869510724661
23	22.508631193614555	26.2636627541407	31.086986157330255	20.14071989491449
24	21.804926041740423	25.97738294389486	32.07443486420995	20.143256150154766
25	22.486121928357132	26.97127796617692	30.30877850777309	20.233821597692852
26	22.147848885685693	27.18125876461121	29.551706318551545	21.119186031151553
27	21.5729643645571	26.957645594260455	31.102520720676925	20.36686932050552
28	20.255908153743565	26.721245470406657	31.309753909267585	21.713092466582196
29	21.391516437575888	26.943590513137273	30.894970500181234	20.769922549105598
30	21.93226719026247	26.079150185410825	31.596562106021807	20.392020518304893
31	23.281872009992846	26.257744825246732	29.944614526190534	20.515768638569888
32	23.250380174092786	26.5616727448728	29.896425676625345	20.291521404409067
33	22.909148166974365	26.62021797000244	29.629273457983235	20.841360405039964
34	20.97472515975766	27.16371633253265	31.61812027556413	20.243438232145554
35	21.786326836645085	27.755192190024697	30.464863882351572	19.993617090978645
36	23.354895025452375	27.727082027778337	28.616884907907515	20.301138038861772
37	22.074508838321126	28.316655693840175	29.303258982306453	20.30557648553225
38	22.50683467948603	28.639816882371655	28.961709943282994	19.89163849485933
39	21.874673060847933	27.519320452679292	29.397417458101593	21.208589028371186
40	23.31632281033989	26.81329040016824	29.216709272232126	20.653677517259744
41	21.514841848634173	26.894344890555306	29.32006167327326	22.270751587537266
42	22.42662560751239	26.80779518048098	29.09348953847552	21.67208967353111
43	22.298122008671882	26.884833933404277	29.445500630365107	21.371543427558738
44	22.26134630768792	27.16984561602998	29.407985188269397	21.160822888012706
45	22.10135087294735	27.000339224138386	28.79009000535784	22.108219897556424
46	21.36076434278758	27.580296255747523	28.89629569354428	22.16264370792062
47	21.426495624431325	27.705840890141047	29.651571368637303	21.21609211679033
48	21.63489126334044	27.316842742664143	29.95254032381639	21.09572567017903
49	22.559567653023375	27.174812449208847	29.230870030656988	21.034749867110794
50	20.965214202606635	28.449386384747804	29.36708807251999	21.218311340125563
51	21.63045281666996	27.01249211383136	29.06918375908957	22.28787131040911
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	461.0
1	1165.0
2	1869.0
3	8625.0
4	15381.0
5	10492.0
6	5603.0
7	5018.0
8	4433.0
9	4329.0
10	4225.0
11	4151.0
12	4077.0
13	4072.0
14	4067.0
15	3944.0
16	3821.0
17	3771.0
18	3721.0
19	3858.0
20	3995.0
21	4303.0
22	4611.0
23	5295.0
24	5979.0
25	7445.0
26	10245.0
27	11579.0
28	13564.5
29	15550.0
30	17955.5
31	20361.0
32	23113.0
33	25865.0
34	29538.5
35	33212.0
36	36861.5
37	40511.0
38	44279.0
39	48047.0
40	50823.5
41	53600.0
42	56047.0
43	58494.0
44	60532.5
45	62571.0
46	65483.0
47	68395.0
48	68816.0
49	69237.0
50	67435.0
51	65633.0
52	62307.5
53	58982.0
54	55371.0
55	51760.0
56	48711.0
57	45662.0
58	42244.0
59	38826.0
60	35146.5
61	31467.0
62	28132.5
63	24798.0
64	21285.5
65	17773.0
66	15088.0
67	12403.0
68	10311.5
69	8220.0
70	6593.0
71	4966.0
72	4079.0
73	3192.0
74	2529.5
75	1404.0
76	941.0
77	744.0
78	547.0
79	399.5
80	252.0
81	194.5
82	137.0
83	120.0
84	103.0
85	89.0
86	75.0
87	59.0
88	43.0
89	36.5
90	30.0
91	20.0
92	10.0
93	7.0
94	4.0
95	3.5
96	3.0
97	3.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	946277.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.73837142337432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.48105882515713	25.52612314051126
2	6.968474460055145	4.8414690809538685
3	3.2086605099208376	3.3439092169543114
4	2.1265239145856363	2.9548791034225506
5	1.6256918870575603	2.8236944296285915
6	1.3613994745471423	2.8375680361443156
7	1.1530882218068819	2.803948485313199
8	1.0546303841646532	2.9308913599590136
9	0.9277403891591006	2.9005371200767205
>10	8.051024845782177	46.73889578386089
>50	0.02902814932444709	0.6575400189118861
>100	0.011677969615139708	0.609669536501512
>500	3.3365627471681954E-4	0.06629866442543514
>1k	3.3365627471681954E-4	0.3495747760613852
>5k	3.3365627471681954E-4	0.6150012472751029
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5306	0.5607237627037326	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3016	0.3187227418609984	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018493527793658728	0.0
2	0.0	0.0	0.0	0.07756713943168861	0.0
3	0.0	0.0	0.0	0.10451485135959132	0.0
4	0.0	0.0	0.0	0.13452720503615748	0.0
5	0.0	0.0	0.0	0.23238438639003167	0.0
6	0.0	0.0	0.0	0.35623818395670614	0.0
7	0.0	0.0	0.0	0.47121508818242436	0.0
8	0.0	0.0	0.0	0.733083441740632	0.0
9	0.0	0.0	0.0	0.868984451698604	0.0
10	0.0	0.0	0.0	1.1099286995245579	0.0
11	0.0	0.0	0.0	1.400752633742551	0.0
12	0.0	0.0	0.0	1.6183421978976558	0.0
13	0.0	0.0	0.0	1.7258160137042324	0.0
14	0.0	0.0	0.0	1.7791830510516478	0.0
15	0.0	0.0	0.0	1.8615056690588485	0.0
16	0.0	0.0	0.0	2.0293212241235916	0.0
17	0.0	0.0	0.0	2.220808494764218	0.0
18	0.0	0.0	0.0	2.442942182891479	0.0
19	0.0	0.0	0.0	2.582964607614895	0.0
20	0.0	0.0	0.0	2.731652571075911	0.0
21	0.0	0.0	0.0	2.932545121565884	0.0
22	0.0	0.0	0.0	3.1373477322179446	0.0
23	0.0	0.0	0.0	3.3570508424066103	0.0
24	0.0	0.0	0.0	3.5334262589072756	0.0
25	0.0	0.0	0.0	3.7025099415921554	0.0
26	0.0	0.0	0.0	3.8597577664890936	0.0
27	0.0	0.0	0.0	4.040148920453524	0.0
28	0.0	0.0	0.0	4.212085890283712	0.0
29	1.0567730167804988E-4	0.0	0.0	4.411181926645158	0.0
30	1.0567730167804988E-4	0.0	0.0	4.659840617493609	0.0
31	1.0567730167804988E-4	0.0	0.0	4.862846714017143	0.0
32	1.0567730167804988E-4	0.0	0.0	5.0711366756245795	0.0
33	1.0567730167804988E-4	0.0	0.0	5.274882513259859	0.0
34	1.0567730167804988E-4	0.0	0.0	5.488773371856232	0.0
35	1.0567730167804988E-4	0.0	0.0	5.7195725987210935	0.0
36	1.0567730167804988E-4	0.0	0.0	5.9206765038144225	0.0
37	1.0567730167804988E-4	0.0	0.0	6.150524634964181	0.0
38	1.0567730167804988E-4	0.0	0.0	6.3979151981924955	0.0
39	1.0567730167804988E-4	0.0	0.0	6.773915037562997	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGTA	20	7.032654E-4	45.0	41
CGTACCC	25	3.890282E-5	45.0	20
TTTGCGA	25	3.890282E-5	45.0	1
TATCGCG	20	7.032654E-4	45.0	1
CGTTTTA	1275	0.0	43.7647	1
CGTTATT	525	0.0	42.85714	1
CGTTTTT	2425	0.0	41.288662	1
CGTATAG	45	1.927765E-8	40.0	2
AACGATA	35	6.247119E-6	38.571426	44
TATAGCG	35	6.247119E-6	38.571426	1
ATGCGCG	30	1.1397693E-4	37.500004	1
CGCTTAG	30	1.1397693E-4	37.500004	2
TAGTACG	30	1.1397693E-4	37.500004	1
TAACGGG	225	0.0	37.0	3
CGTTAAG	55	2.748493E-9	36.818184	2
CGCACGG	110	0.0	36.818184	2
ACGGGAT	365	0.0	36.369865	5
AGTACGG	75	1.8189894E-12	36.000004	2
CGTGAAT	25	0.002106921	36.0	27
GGCGTAA	25	0.002106921	36.0	1
>>END_MODULE