##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934599.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444391
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04479613673544	33.0	31.0	34.0	30.0	34.0
2	32.244732679104665	33.0	31.0	34.0	30.0	34.0
3	32.14241512541883	33.0	31.0	34.0	30.0	34.0
4	35.86935604006382	37.0	35.0	37.0	35.0	37.0
5	32.144728403590534	37.0	35.0	37.0	0.0	37.0
6	34.0170570511104	37.0	35.0	37.0	19.0	37.0
7	20.71165707676348	32.0	0.0	37.0	0.0	37.0
8	28.096210319290893	35.0	17.0	37.0	17.0	37.0
9	34.886172312220545	37.0	32.0	39.0	32.0	39.0
10	36.30172753273581	37.0	35.0	39.0	32.0	39.0
11	36.8203744000216	39.0	37.0	39.0	33.0	39.0
12	37.13464944159535	39.0	37.0	39.0	34.0	39.0
13	36.888190804944294	39.0	37.0	39.0	33.0	39.0
14	38.28206916881755	40.0	38.0	41.0	34.0	41.0
15	38.46441984648654	40.0	38.0	41.0	34.0	41.0
16	38.543719382255716	40.0	38.0	41.0	34.0	41.0
17	38.486152959893424	40.0	38.0	41.0	34.0	41.0
18	38.41288189904836	40.0	38.0	41.0	34.0	41.0
19	38.375750183959624	40.0	37.0	41.0	34.0	41.0
20	38.36472385804393	40.0	37.0	41.0	34.0	41.0
21	38.29379982942949	40.0	37.0	41.0	34.0	41.0
22	38.27846873586549	40.0	37.0	41.0	34.0	41.0
23	38.21520912889775	40.0	37.0	41.0	34.0	41.0
24	38.132628698601	40.0	37.0	41.0	34.0	41.0
25	38.01448274154967	40.0	37.0	41.0	33.0	41.0
26	37.877655038018325	40.0	37.0	41.0	33.0	41.0
27	37.82839886496351	40.0	37.0	41.0	33.0	41.0
28	37.75247473508689	40.0	36.0	41.0	33.0	41.0
29	37.7429470893875	40.0	36.0	41.0	33.0	41.0
30	37.6137613047969	40.0	36.0	41.0	33.0	41.0
31	37.5232239176761	40.0	36.0	41.0	33.0	41.0
32	37.45239215015606	40.0	36.0	41.0	33.0	41.0
33	37.3379298860688	40.0	36.0	41.0	32.0	41.0
34	37.22934307850519	40.0	36.0	41.0	32.0	41.0
35	37.11070881273473	40.0	36.0	41.0	31.0	41.0
36	36.98372154251549	40.0	36.0	41.0	31.0	41.0
37	36.911492356955925	40.0	35.0	41.0	31.0	41.0
38	36.7969715858332	39.0	35.0	41.0	31.0	41.0
39	36.75985337236803	39.0	35.0	41.0	31.0	41.0
40	36.69598169179844	39.0	35.0	41.0	30.0	41.0
41	36.58231827377242	39.0	35.0	41.0	30.0	41.0
42	36.4951450411912	39.0	35.0	41.0	30.0	41.0
43	36.389177548600216	39.0	35.0	41.0	30.0	41.0
44	36.25114144975933	39.0	35.0	40.0	29.0	41.0
45	36.18468645854664	39.0	35.0	40.0	29.0	41.0
46	36.063169596143936	39.0	35.0	40.0	29.0	41.0
47	36.04414355826288	39.0	35.0	40.0	29.0	41.0
48	35.92030441660609	39.0	35.0	40.0	28.0	41.0
49	35.81185712581938	39.0	35.0	40.0	28.0	41.0
50	35.6645656640211	38.0	35.0	40.0	27.0	41.0
51	33.799172350475146	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	19.0
10	24.0
11	16.0
12	9.0
13	9.0
14	8.0
15	16.0
16	26.0
17	63.0
18	108.0
19	238.0
20	378.0
21	671.0
22	1041.0
23	1636.0
24	2296.0
25	3264.0
26	4103.0
27	4601.0
28	5545.0
29	6389.0
30	8116.0
31	10385.0
32	14122.0
33	20219.0
34	28639.0
35	37410.0
36	49643.0
37	87787.0
38	94791.0
39	62798.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.412814390930514	19.987803533374887	27.183268788071768	13.416113287622835
2	30.350749677648736	24.69784491585113	27.515858782018537	17.435546624481592
3	32.1635676690122	24.27141863809123	27.431473634704574	16.133540058191997
4	28.85296956959074	26.042381596386967	26.763368295037477	18.341280538984815
5	22.628496076653217	37.73298739173386	22.480878325618654	17.15763820599427
6	27.478054236021883	35.40148202821389	23.475272901566413	13.645190834197813
7	50.08224739024868	43.168741041110195	4.4775884300087085	2.2714231386324206
8	87.01391342308912	4.449685074630224	6.198595381094576	2.3378061211860723
9	79.49688450036116	8.668267359149938	7.740705819874839	4.094142320614054
10	34.34385484854553	40.42746140223362	13.882819409033937	11.345864340186909
11	32.69935709769099	25.90759038774413	24.781104927867574	16.6119475866973
12	31.803074319686942	22.50990681629466	26.98209459687527	18.704924267143124
13	25.852233731106168	24.873591049323682	28.99698688767324	20.277188331896912
14	20.790250027565815	27.986615390500706	29.18330929294248	22.039825288991004
15	20.58480032223875	27.0009968698736	32.612271625662984	19.801931182224664
16	24.713146755897398	24.712696701778388	31.238481427391644	19.33567511493257
17	23.302452119867414	24.85221347867081	29.640339250794906	22.204995150666868
18	23.807862895513185	25.30631808474969	29.628637843700705	21.25718117603642
19	24.75095130189405	26.30138774187596	28.340807982159856	20.60685297407013
20	26.31736466310074	25.762447934364108	28.7584582045991	19.16172919793605
21	26.187524049766985	25.379676906147964	28.92925374276257	19.503545301322482
22	24.51107245646289	24.369755463094435	29.40181056772077	21.717361512721904
23	22.797941452459657	26.71971304549372	29.185334536478013	21.29701096556861
24	22.33078527692955	26.171772155601715	30.025360549606088	21.472082017862647
25	21.721411999792974	27.765863845127374	28.517679250929923	21.995044904149726
26	22.31008278745519	29.065620140821935	27.764063628651346	20.86023344307153
27	21.7574163293136	27.878602401938835	29.020614728921153	21.343366539826413
28	21.20474987117201	27.3522641097592	30.760298925945843	20.682687093122947
29	21.76079173520616	26.815574572842383	30.31654556460414	21.107088127347314
30	22.870175138560413	25.464512107580937	30.283691613916574	21.381621139942077
31	23.54075577588205	26.258407573510716	28.338782738624317	21.862053911982915
32	24.124025914116174	27.46477763951115	28.63334315951493	19.777853286857745
33	23.21581670195841	27.356314596830266	28.203991529981483	21.22387717122984
34	22.401218746554274	27.145689269134614	29.262968872006862	21.190123112304253
35	23.20073988897165	26.370921103262667	28.48640048965888	21.941938518106802
36	23.21581670195841	27.955336629229667	27.93373403151729	20.895112637294634
37	22.652573972020136	28.677898517296708	28.262723592512	20.40680391817116
38	22.303331975670073	28.210067260588083	28.08292697196838	21.40367379177346
39	22.352837928760934	27.18641916690482	28.422042750640763	22.038700153693483
40	22.898753575117407	26.047107164636547	29.150905396373915	21.90323386387213
41	20.517292204387577	27.61397057996224	29.165082101122657	22.70365511452752
42	21.55939251695016	27.32211048378568	28.179013526376544	22.939483472887616
43	22.854648271454643	26.60224892043268	28.895049629717974	21.6480531783947
44	21.92281121804897	27.40739573933765	28.898650062670033	21.77114297994334
45	22.28803013562381	27.6634765330531	27.27507982834936	22.77341350297373
46	21.441028283651107	28.40291545058293	28.0669500507436	22.089106215022355
47	21.80399693063091	27.253252203577482	29.323951205132413	21.618799660659192
48	22.00989669007698	26.232529461667763	29.841288414931892	21.916285433323356
49	21.523838241548546	26.647929413511974	29.001262401803817	22.826969943135662
50	20.791825216982343	27.409195955813686	29.487545877391756	22.311432949812215
51	20.430431759419072	26.82457565522254	29.416437326588525	23.328555258769867
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	303.0
1	774.5
2	1246.0
3	2386.0
4	3526.0
5	2525.0
6	1524.0
7	1377.0
8	1230.0
9	1227.5
10	1225.0
11	1147.0
12	1069.0
13	1037.5
14	1006.0
15	1092.5
16	1179.0
17	1155.0
18	1131.0
19	1176.0
20	1221.0
21	1500.0
22	1779.0
23	2138.0
24	2497.0
25	3160.0
26	4842.5
27	5862.0
28	7079.5
29	8297.0
30	9445.5
31	10594.0
32	11749.5
33	12905.0
34	14585.5
35	16266.0
36	17409.0
37	18552.0
38	19870.5
39	21189.0
40	23162.5
41	25136.0
42	26949.0
43	28762.0
44	29642.0
45	30522.0
46	31539.0
47	32556.0
48	32458.5
49	32361.0
50	31516.5
51	30672.0
52	29119.5
53	27567.0
54	25480.0
55	23393.0
56	22447.5
57	21502.0
58	21041.0
59	20580.0
60	19282.5
61	17985.0
62	16080.5
63	14176.0
64	11601.5
65	9027.0
66	7269.0
67	5511.0
68	4427.0
69	3343.0
70	2671.5
71	2000.0
72	1600.0
73	1200.0
74	1005.5
75	605.5
76	400.0
77	304.0
78	208.0
79	159.5
80	111.0
81	86.5
82	62.0
83	44.5
84	27.0
85	27.5
86	28.0
87	20.5
88	13.0
89	11.5
90	10.0
91	5.5
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	444391.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.856345220400016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.67440862036659	22.235337322171794
2	7.788180553130511	5.11782297784924
3	4.19145826802172	4.131479994930647
4	3.077912817214375	4.045158643227579
5	2.3909898237668057	3.927959353407278
6	1.905204461233651	3.7558833296243423
7	1.6475814202250263	3.7893452745141283
8	1.4582380065046407	3.832989708417952
9	1.2304397630959576	3.6384978266269195
>10	8.585811640754727	43.798121343456074
>50	0.03525702652760712	0.761148134197092
>100	0.013134970667147753	0.5919283908438143
>500	6.913142456393554E-4	0.12629017087841934
>1k	6.913142456393554E-4	0.24803752985473726
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1092	0.24572954897826463	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	556	0.12511504508417137	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.500541190078107E-4	0.0	0.0	0.01980238123634367	0.0
2	4.500541190078107E-4	0.0	0.0	0.10778796150237066	0.0
3	4.500541190078107E-4	0.0	0.0	0.14559250749902675	0.0
4	4.500541190078107E-4	0.0	0.0	0.19622359588740546	0.0
5	4.500541190078107E-4	0.0	0.0	0.34204113044593615	0.0
6	4.500541190078107E-4	0.0	0.0	0.42755141305742017	0.0
7	4.500541190078107E-4	0.0	0.0	0.5256632110011229	0.0
8	4.500541190078107E-4	0.0	0.0	0.808297197738028	0.0
9	4.500541190078107E-4	0.0	0.0	0.9100094286337932	0.0
10	4.500541190078107E-4	0.0	0.0	1.2565511002698075	0.0
11	4.500541190078107E-4	0.0	0.0	1.656874239127255	0.0
12	4.500541190078107E-4	0.0	0.0	1.9980152613351756	0.0
13	4.500541190078107E-4	0.0	0.0	2.1361818758705735	0.0
14	4.500541190078107E-4	0.0	0.0	2.189738316032503	0.0
15	4.500541190078107E-4	0.0	0.0	2.3031519540224714	0.0
16	4.500541190078107E-4	0.0	0.0	2.5338046900139743	0.0
17	4.500541190078107E-4	0.0	0.0	2.7970863496335436	0.0
18	4.500541190078107E-4	0.0	0.0	3.078595201072929	0.0
19	4.500541190078107E-4	0.0	0.0	3.2466904145223463	0.0
20	4.500541190078107E-4	0.0	0.0	3.4489897410163572	0.0
21	4.500541190078107E-4	0.0	0.0	3.706195670029321	0.0
22	4.500541190078107E-4	0.0	0.0	3.9737528437794647	0.0
23	4.500541190078107E-4	0.0	0.0	4.234559205744491	0.0
24	4.500541190078107E-4	0.0	0.0	4.446759722856673	0.0
25	4.500541190078107E-4	0.0	0.0	4.642533264625071	0.0
26	4.500541190078107E-4	0.0	0.0	4.825030209882739	0.0
27	6.75081178511716E-4	0.0	0.0	5.008652290437925	0.0
28	6.75081178511716E-4	0.0	0.0	5.207801238098881	0.0
29	6.75081178511716E-4	0.0	0.0	5.408300348116861	0.0
30	6.75081178511716E-4	0.0	0.0	5.636927840572829	0.0
31	6.75081178511716E-4	0.0	0.0	5.864430197731277	0.0
32	6.75081178511716E-4	0.0	0.0	6.075505579545941	0.0
33	6.75081178511716E-4	0.0	0.0	6.27195420249285	0.0
34	6.75081178511716E-4	0.0	0.0	6.499231532591794	0.0
35	6.75081178511716E-4	0.0	0.0	6.724258592095699	0.0
36	6.75081178511716E-4	0.0	0.0	6.968187924597933	0.0
37	6.75081178511716E-4	0.0	0.0	7.208066770029096	0.0
38	6.75081178511716E-4	0.0	0.0	7.455146481364384	0.0
39	6.75081178511716E-4	0.0	0.0	7.697950678569098	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.02861E-4	45.000004	6
ACACGAA	20	7.02861E-4	45.000004	15
CGGCCTA	20	7.02861E-4	45.000004	45
TCGATGG	20	7.02861E-4	45.000004	2
ACGGATT	20	7.02861E-4	45.000004	26
CCACACG	20	7.02861E-4	45.000004	13
TCGTACG	50	2.1827873E-11	45.0	30
ACGGGTA	30	2.1624855E-6	44.999996	5
CGTTTTA	230	0.0	41.08696	1
TAGGACC	100	0.0	40.5	6
CGAATAT	40	3.4518416E-7	39.375004	14
CGTAAAG	40	3.4518416E-7	39.375004	2
AGGATAC	40	3.4518416E-7	39.375004	7
TCACGAC	75	0.0	39.000004	25
TATGCGG	35	6.239643E-6	38.571426	2
CTAGCGA	35	6.239643E-6	38.571426	1
CATACGA	100	0.0	38.25	18
TAAGGAT	295	0.0	38.135593	5
TACGGGA	160	0.0	37.968754	4
TTAGGAT	155	0.0	37.741936	5
>>END_MODULE