##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934598.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518487
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90973737046445	31.0	31.0	34.0	30.0	34.0
2	32.10052325323489	33.0	31.0	34.0	30.0	34.0
3	31.98443355378245	33.0	31.0	34.0	30.0	34.0
4	35.7513071687429	37.0	35.0	37.0	35.0	37.0
5	32.096123914389366	37.0	35.0	37.0	10.0	37.0
6	33.94933527745151	37.0	35.0	37.0	19.0	37.0
7	20.740442865491325	32.0	0.0	37.0	0.0	37.0
8	28.05803038456123	35.0	17.0	37.0	17.0	37.0
9	34.772165936658006	37.0	32.0	39.0	31.0	39.0
10	36.1611997986449	37.0	35.0	39.0	32.0	39.0
11	36.59114886197725	38.0	35.0	39.0	32.0	39.0
12	36.98512209563596	39.0	37.0	39.0	33.0	39.0
13	36.78206589557694	39.0	37.0	39.0	33.0	39.0
14	38.18512132416049	40.0	37.0	41.0	33.0	41.0
15	38.33979251167339	40.0	38.0	41.0	34.0	41.0
16	38.41995074129149	40.0	38.0	41.0	34.0	41.0
17	38.38475024446129	40.0	38.0	41.0	34.0	41.0
18	38.28309099360254	40.0	37.0	41.0	34.0	41.0
19	38.243346506276914	40.0	37.0	41.0	34.0	41.0
20	38.24697244096766	40.0	37.0	41.0	34.0	41.0
21	38.191069400004245	40.0	37.0	41.0	34.0	41.0
22	38.175697751341886	40.0	37.0	41.0	34.0	41.0
23	38.12781034047141	40.0	37.0	41.0	34.0	41.0
24	38.019931068667105	40.0	37.0	41.0	34.0	41.0
25	37.89201272163044	40.0	36.0	41.0	33.0	41.0
26	37.76013863414126	40.0	36.0	41.0	33.0	41.0
27	37.73160754271563	40.0	36.0	41.0	33.0	41.0
28	37.66186808926743	40.0	36.0	41.0	33.0	41.0
29	37.68227361534619	40.0	36.0	41.0	33.0	41.0
30	37.55288753623524	40.0	36.0	41.0	33.0	41.0
31	37.461259395124664	40.0	36.0	41.0	33.0	41.0
32	37.37734793736391	40.0	36.0	41.0	33.0	41.0
33	37.23677739268294	40.0	36.0	41.0	32.0	41.0
34	37.102714243558665	40.0	36.0	41.0	31.0	41.0
35	36.99925938355253	40.0	36.0	41.0	31.0	41.0
36	36.82947113428109	39.0	35.0	41.0	31.0	41.0
37	36.75539020264732	39.0	35.0	41.0	31.0	41.0
38	36.67140545471728	39.0	35.0	41.0	30.0	41.0
39	36.613639300503195	39.0	35.0	41.0	30.0	41.0
40	36.494849436919345	39.0	35.0	41.0	30.0	41.0
41	36.423792689112744	39.0	35.0	40.0	30.0	41.0
42	36.31465205492134	39.0	35.0	40.0	30.0	41.0
43	36.22308948922538	39.0	35.0	40.0	30.0	41.0
44	36.099113381820565	39.0	35.0	40.0	29.0	41.0
45	36.019628264546654	39.0	35.0	40.0	29.0	41.0
46	35.87216458657594	39.0	35.0	40.0	28.0	41.0
47	35.79290705456453	39.0	35.0	40.0	28.0	41.0
48	35.687741447712284	38.0	35.0	40.0	27.0	41.0
49	35.55820300219678	38.0	35.0	40.0	27.0	41.0
50	35.40108430876763	38.0	34.0	40.0	26.0	41.0
51	33.45442412249488	36.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	27.0
10	32.0
11	12.0
12	10.0
13	13.0
14	14.0
15	14.0
16	31.0
17	57.0
18	135.0
19	246.0
20	436.0
21	725.0
22	1201.0
23	1979.0
24	2987.0
25	4114.0
26	5252.0
27	5910.0
28	6812.0
29	8097.0
30	10007.0
31	13028.0
32	17623.0
33	25134.0
34	35297.0
35	45351.0
36	59526.0
37	99567.0
38	105405.0
39	69422.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.15136541514059	21.969113979714052	26.930472702304975	13.94904790284038
2	29.40941624380168	25.705369662113032	27.2290337076918	17.656180386393487
3	31.53039516902063	24.709009097624435	28.08517860621385	15.675417127141086
4	27.365970602927366	26.02128886548747	27.718534890942298	18.89420564064287
5	21.97952889850662	37.067081720467435	23.13847791747913	17.81491146354682
6	25.67296769253617	35.094804691342304	25.07391699309722	14.1583106230243
7	48.97789144182978	42.97291928245067	5.316044568137677	2.733144707581868
8	83.94733136992826	5.307365469143875	7.374341111734721	3.370962049193133
9	78.12732045355042	7.869628360981086	8.981131638787472	5.0219195466810165
10	37.49332191549644	33.67220393182471	15.98014993625684	12.854324216422011
11	29.262257298640083	26.020131652288292	26.641555140244595	18.07605590882703
12	27.85007145791505	23.61389967347301	29.21133991787644	19.324688950735506
13	23.001733891110096	26.625354155456165	30.854582660703162	19.51832929273058
14	19.456611255441313	29.517808546790953	29.600549290531873	21.42503090723586
15	18.477416020073793	28.402255022787458	34.15437609814711	18.965952858991646
16	21.55483165440985	26.547628002244995	32.41180588905797	19.485734454287183
17	21.30255917698997	25.77229515879858	29.664196016486432	23.260949647725017
18	21.2880940120003	26.640012189312362	31.465398361000375	20.606495437686963
19	22.691022147131942	27.917575561200188	28.22578000991346	21.165622281754413
20	23.793460588211467	27.12102714243559	29.96063546434144	19.124876805011503
21	22.847631666753458	28.01073122373367	29.54442445037195	19.597212659140922
22	22.07712054497027	25.67798228306592	29.013842198550783	23.231054973413027
23	20.835045044523778	27.74804382752123	29.602670848063696	21.8142402798913
24	20.322785335022864	26.91272876658431	31.371664091867295	21.392821806525525
25	20.711608969945246	28.666485369932133	29.165051389909486	21.45685427021314
26	20.45682919726049	29.68136134560751	28.028475159454334	21.833334297677666
27	20.98278259628496	28.901785387097455	29.48849247907855	20.626939537539034
28	18.61473865304241	29.04894433225905	31.364527943805726	20.97178907089281
29	19.435974286722715	27.514093892421602	30.589387969225847	22.46054385162984
30	20.501574774295207	28.220958288250237	29.480199117817808	21.79726781963675
31	22.051468985721918	27.859714901241496	27.41438068842613	22.674435424610454
32	21.770651916055755	28.782399558716033	26.952652621955806	22.494295903272405
33	20.94363021637958	29.463805264162847	27.491528235037716	22.10103628441986
34	20.14611745328234	28.337258214767196	28.387404120064723	23.129220211885738
35	20.355187304599728	27.562118240187317	27.87244424643241	24.210250208780547
36	20.785670614692364	30.26999712625389	27.34996248700546	21.594369772048285
37	19.883430057069898	29.23294123092768	29.026378674875165	21.85725003712726
38	20.306198612501376	28.864947433590427	27.74977964731999	23.079074306588208
39	21.379706723601554	28.012081305799374	28.294441326397767	22.3137706442013
40	21.06070161836266	27.575040454244753	28.857811285528857	22.506446641863732
41	19.04985081593174	27.76058030384561	29.018278182480948	24.1712906977417
42	20.38141747044767	28.118544920123355	28.459151338413502	23.040886271015474
43	21.168515314752348	27.778709977299332	28.619811104232124	22.432963603716196
44	20.104072040379027	28.164640579223782	28.139760495441546	23.59152688495565
45	19.843313332831876	28.03117532358574	27.621521851078235	24.503989492504154
46	20.68094282016714	29.209796966944207	27.104247551047568	23.005012661841086
47	20.502539118627855	27.630200950072037	29.35001263291076	22.517247298389353
48	20.309477383232366	26.981582951935152	28.833895546079262	23.87504411875322
49	19.81669742925088	26.95921016341779	30.004223828176986	23.219868579154348
50	19.33105362333096	27.532609303608385	29.366406486565722	23.769930586494937
51	18.86662539272923	28.083828524148146	27.639844393398484	25.40970168972414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	276.0
1	747.0
2	1218.0
3	3032.5
4	4847.0
5	3477.5
6	2108.0
7	1852.0
8	1596.0
9	1562.5
10	1529.0
11	1495.0
12	1461.0
13	1473.5
14	1486.0
15	1458.0
16	1430.0
17	1522.5
18	1615.0
19	1742.5
20	1870.0
21	2186.5
22	2503.0
23	2955.0
24	3407.0
25	4312.0
26	6171.0
27	7125.0
28	8753.0
29	10381.0
30	11689.5
31	12998.0
32	14509.0
33	16020.0
34	17450.5
35	18881.0
36	20739.5
37	22598.0
38	24845.0
39	27092.0
40	29136.5
41	31181.0
42	32911.5
43	34642.0
44	37721.5
45	40801.0
46	43322.5
47	45844.0
48	43382.5
49	40921.0
50	38677.5
51	36434.0
52	33182.5
53	29931.0
54	26785.0
55	23639.0
56	22286.0
57	20933.0
58	20052.0
59	19171.0
60	17584.0
61	15997.0
62	13889.0
63	11781.0
64	9955.5
65	8130.0
66	6551.5
67	4973.0
68	4016.0
69	3059.0
70	2642.5
71	2226.0
72	1777.5
73	1329.0
74	1064.5
75	624.0
76	448.0
77	345.5
78	243.0
79	202.5
80	162.0
81	134.5
82	107.0
83	74.0
84	41.0
85	27.0
86	13.0
87	12.5
88	12.0
89	8.0
90	4.0
91	4.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	518487.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.815485952159676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.30458091126928	26.98743768767786
2	8.104361536492984	5.967320165959614
3	3.5291233754768907	3.897791761599234
4	2.268744342852664	3.3409970193333596
5	1.6914474800808739	3.113573047086665
6	1.3271213766172654	2.9315171038598247
7	1.1065722612118993	2.8517296876387372
8	0.9735584351244617	2.8673621513544942
9	0.8587804371070044	2.8454777206472217
>10	6.7608063795408455	41.47579636526479
>50	0.046547152768913885	1.1073763571018422
>100	0.025146162990098204	1.6128064055404696
>500	0.0026751237223508724	0.6921594661784575
>1k	5.350247444701745E-4	0.3086550607574396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1567	0.30222551385087765	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	864	0.16663870068101996	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	824	0.15892394601986162	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	737	0.14214435463184227	No Hit
GAATCTATCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	564	0.10877804072233248	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	525	0.10125615492770311	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.857377330579166E-4	0.0	0.0	0.05573910242686895	0.0
2	3.857377330579166E-4	0.0	0.0	0.28467444699674244	0.0
3	3.857377330579166E-4	0.0	0.0	0.37937306046246094	0.0
4	3.857377330579166E-4	0.0	0.0	0.5114882340347974	0.0
5	3.857377330579166E-4	0.0	0.0	0.9830526126981004	0.0
6	3.857377330579166E-4	0.0	0.0	1.2181597609969006	0.0
7	3.857377330579166E-4	0.0	0.0	1.4740967468808281	0.0
8	3.857377330579166E-4	0.0	0.0	2.1068994979623406	0.0
9	3.857377330579166E-4	0.0	0.0	2.2885819702326193	0.0
10	3.857377330579166E-4	0.0	0.0	3.0419277628947303	0.0
11	3.857377330579166E-4	0.0	0.0	3.7075182212861653	0.0
12	3.857377330579166E-4	0.0	0.0	4.433669503767693	0.0
13	3.857377330579166E-4	0.0	0.0	4.670705340731783	0.0
14	3.857377330579166E-4	0.0	0.0	4.768682724928494	0.0
15	3.857377330579166E-4	0.0	0.0	4.936285769942159	0.0
16	3.857377330579166E-4	0.0	0.0	5.266284400573206	0.0
17	3.857377330579166E-4	0.0	0.0	5.703518120994355	0.0
18	3.857377330579166E-4	0.0	0.0	6.157338563936993	0.0
19	3.857377330579166E-4	0.0	0.0	6.470750472046551	0.0
20	3.857377330579166E-4	0.0	0.0	6.77085442836561	0.0
21	3.857377330579166E-4	0.0	0.0	7.171057326413198	0.0
22	3.857377330579166E-4	0.0	0.0	7.600962029906246	0.0
23	3.857377330579166E-4	0.0	0.0	8.078505343431948	0.0
24	3.857377330579166E-4	0.0	0.0	8.421811925853493	0.0
25	3.857377330579166E-4	0.0	0.0	8.754317851749416	0.0
26	3.857377330579166E-4	0.0	0.0	9.034749183682523	0.0
27	3.857377330579166E-4	0.0	0.0	9.330224287204887	0.0
28	3.857377330579166E-4	0.0	0.0	9.652701032041305	0.0
29	3.857377330579166E-4	0.0	0.0	9.963219907152927	0.0
30	3.857377330579166E-4	0.0	0.0	10.33661403275299	0.0
31	3.857377330579166E-4	0.0	0.0	10.673170204846024	0.0
32	3.857377330579166E-4	0.0	0.0	11.003747442076657	0.0
33	3.857377330579166E-4	0.0	0.0	11.327574268978779	0.0
34	3.857377330579166E-4	0.0	0.0	11.620542077236266	0.0
35	3.857377330579166E-4	0.0	0.0	11.990850300971866	0.0
36	3.857377330579166E-4	0.0	0.0	12.319498849537212	0.0
37	3.857377330579166E-4	0.0	0.0	12.653933464098424	0.0
38	3.857377330579166E-4	0.0	0.0	12.968888323140213	0.0
39	3.857377330579166E-4	0.0	0.0	13.294258100974567	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATTC	30	2.1631877E-6	45.000004	9
CGTGGCT	25	3.8878337E-5	45.0	34
GACGTAG	20	7.0297E-4	45.0	2
CCGCTTA	20	7.0297E-4	45.0	19
TGTGCGA	40	6.8030204E-9	45.0	1
CCCGTAC	20	7.0297E-4	45.0	10
TGCGATA	20	7.0297E-4	45.0	1
GGGCGTA	20	7.0297E-4	45.0	7
TGCGACA	25	3.8878337E-5	45.0	1
TGCGAAA	20	7.0297E-4	45.0	1
CGTACTA	20	7.0297E-4	45.0	31
TATAGCG	25	3.8878337E-5	45.0	1
TTACCGC	20	7.0297E-4	45.0	42
CGTTTTA	325	0.0	42.23077	1
CGTTATT	155	0.0	42.09677	1
AGGCGAT	340	0.0	41.02941	7
TACGGGT	55	6.002665E-11	40.90909	4
TACGGGA	260	0.0	40.67308	4
AAGGCGA	175	0.0	39.857143	6
TAGGCGA	130	0.0	39.807693	6
>>END_MODULE