FastQCFastQC Report
Sat 14 Jan 2017
SRR2934597.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934597.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834419
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45310.5430125632326205No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25130.30116763880017117No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.11421120564129052No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG207.0321735E-445.00000445
TCGACGA207.0321735E-445.0000041
CTAGCGT207.0321735E-445.00000444
CGTTTTA11750.042.893621
CGTTATT5100.041.9117661
CGTTTTT20250.041.777781
ACGTTAG603.6379788E-1241.2500042
TCACGAC451.9272193E-840.025
GCGACGC356.2462314E-638.57142619
CGTATAG356.2462314E-638.5714262
TCGATAG356.2462314E-638.5714262
AGGCGAT3650.037.602747
CAACCCG301.1396535E-437.50000413
CGAGACA301.1396535E-437.50000422
ACCCGGT301.1396535E-437.50000415
ACGGTTC301.1396535E-437.50000438
TATGCGG850.037.0588232
AATCCGT250.002106778336.020
CCGTATG250.002106778336.039
GCCGATC250.002106778336.016
Produced by FastQC (version 0.11.5)