##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934597.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	834419
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.834240351669845	31.0	31.0	34.0	30.0	34.0
2	32.0315285246381	33.0	31.0	34.0	30.0	34.0
3	31.787100964862976	33.0	31.0	34.0	30.0	34.0
4	35.63167665165822	37.0	35.0	37.0	33.0	37.0
5	31.942660701637905	37.0	35.0	37.0	0.0	37.0
6	33.860238081827	37.0	35.0	37.0	19.0	37.0
7	20.8770917249008	32.0	0.0	37.0	0.0	37.0
8	28.15296152172949	35.0	17.0	37.0	17.0	37.0
9	34.79180843197482	37.0	32.0	39.0	32.0	39.0
10	36.09311988341589	37.0	35.0	39.0	32.0	39.0
11	36.579048415723996	38.0	35.0	39.0	32.0	39.0
12	36.981633927319486	39.0	37.0	39.0	33.0	39.0
13	36.81828194228559	39.0	37.0	39.0	33.0	39.0
14	38.15268468239577	40.0	37.0	41.0	33.0	41.0
15	38.32860109848889	40.0	38.0	41.0	34.0	41.0
16	38.37239084920166	40.0	38.0	41.0	34.0	41.0
17	38.36860857674621	40.0	38.0	41.0	34.0	41.0
18	38.2500314590152	40.0	37.0	41.0	34.0	41.0
19	38.18203444552437	40.0	37.0	41.0	34.0	41.0
20	38.137675436441405	40.0	37.0	41.0	34.0	41.0
21	38.05877263101631	40.0	37.0	41.0	34.0	41.0
22	38.02698644206328	40.0	37.0	41.0	34.0	41.0
23	37.951120480238345	40.0	36.0	41.0	33.0	41.0
24	37.83813407892198	40.0	36.0	41.0	33.0	41.0
25	37.69383966568355	40.0	36.0	41.0	33.0	41.0
26	37.55905845864008	39.0	36.0	41.0	33.0	41.0
27	37.529820150308176	39.0	36.0	41.0	33.0	41.0
28	37.443723117522495	39.0	36.0	41.0	33.0	41.0
29	37.4340397330358	39.0	36.0	41.0	33.0	41.0
30	37.27772737677354	39.0	36.0	41.0	32.0	41.0
31	37.15329708455824	39.0	35.0	41.0	32.0	41.0
32	37.03115581021046	39.0	35.0	41.0	31.0	41.0
33	36.85741456031083	39.0	35.0	41.0	31.0	41.0
34	36.65317184771679	39.0	35.0	41.0	30.0	41.0
35	36.47282839916157	39.0	35.0	41.0	30.0	41.0
36	36.291329655724525	39.0	35.0	41.0	30.0	41.0
37	36.20748448920746	39.0	35.0	41.0	29.0	41.0
38	36.07947565911131	39.0	35.0	41.0	29.0	41.0
39	36.00417655877922	39.0	35.0	41.0	28.0	41.0
40	35.906590094424985	39.0	35.0	40.0	27.0	41.0
41	35.79523716502141	39.0	35.0	40.0	27.0	41.0
42	35.685003577339444	39.0	35.0	40.0	26.0	41.0
43	35.623808901762786	39.0	35.0	40.0	26.0	41.0
44	35.5074369111921	39.0	35.0	40.0	26.0	41.0
45	35.42219076986502	39.0	35.0	40.0	25.0	41.0
46	35.30333441592293	38.0	34.0	40.0	24.0	41.0
47	35.2450579385177	38.0	34.0	40.0	24.0	41.0
48	35.09763320346253	38.0	34.0	40.0	24.0	41.0
49	34.97676227410929	38.0	34.0	40.0	24.0	41.0
50	34.78884589157246	38.0	34.0	40.0	23.0	41.0
51	32.84710439239758	36.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	19.0
9	46.0
10	43.0
11	31.0
12	15.0
13	21.0
14	22.0
15	49.0
16	97.0
17	135.0
18	297.0
19	547.0
20	960.0
21	1704.0
22	2993.0
23	4581.0
24	6889.0
25	9459.0
26	11526.0
27	12415.0
28	12977.0
29	14529.0
30	17169.0
31	22165.0
32	28880.0
33	40425.0
34	55542.0
35	70312.0
36	94228.0
37	157937.0
38	161543.0
39	106849.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.39375781232211	20.893819531913824	25.110765694453267	14.601656961310805
2	33.86200458043261	23.005708163404716	26.35474503816428	16.77754221799839
3	30.254824015272902	23.23377104308507	30.44801232953708	16.06339261210495
4	27.03521851731564	25.100938497325686	29.59508352518339	18.268759460175282
5	21.646558863113135	36.785236194286085	25.030829834891104	16.537375107709675
6	25.531297825193338	33.57689601986532	27.24123012539264	13.650576029548702
7	48.013767663488004	42.45972347226034	6.711855794271224	2.8146530699804293
8	82.86879852927605	4.284298416023605	9.593980961603224	3.252922093097113
9	76.63619836077558	7.387175987123975	11.621619354305212	4.355006297795232
10	36.85570438832289	30.171772215158093	19.795810018707627	13.176713377811385
11	29.076638954769724	25.47952527447242	27.381207762526984	18.062628008230877
12	25.97244310112785	23.536376808294154	31.202549318747536	19.28863077183046
13	23.57376809492593	24.025100099590254	32.90349332889112	19.497638476592698
14	20.36446916956589	26.456852013197206	32.49051136179785	20.688167455439054
15	19.881857915507677	26.45577341839052	34.55122666190487	19.11114200419693
16	22.37269285574753	25.65293935061402	32.84812546214791	19.126242331490534
17	22.937037627379052	25.66851905337726	30.792563448339504	20.601879870904185
18	22.870164749364527	24.950534443726713	31.957206151825403	20.22209465508336
19	23.18679224706053	26.67628613442407	29.96168591558917	20.175235702926226
20	24.05494122257523	26.060528343673862	30.603449825567253	19.28108060818366
21	23.840420699912155	26.342281275953688	30.977961911222057	18.839336112912097
22	22.650371096535434	24.20726277805275	30.925709985031503	22.216656140380312
23	20.958295532580156	26.050820990413687	31.903875630828153	21.087007846178
24	21.11061708805768	25.407618954026695	32.245430652945345	21.236333304970284
25	21.27887787790067	26.535709277952684	30.68590240634501	21.499510437801632
26	20.949786617994075	27.621614560550515	30.256022453946997	21.172576367508412
27	20.68457213941677	26.888289935871544	31.291353624498004	21.135784300213682
28	18.819442030922115	27.05367447289671	32.32860229692756	21.798281199253612
29	20.337624143266154	25.25278067733357	31.964516627737382	22.445078551662895
30	20.612426131236226	25.826233582888214	31.941985980664388	21.61935430521117
31	21.26509583314857	25.768588682664223	31.44763002760004	21.518685456587157
32	21.932626174619703	26.023616432511727	30.49031721473265	21.553440178135926
33	21.575970825208916	25.79830996178179	31.333658509693574	21.29206070331572
34	19.586922157812804	26.103192760471654	32.124987566198755	22.184897515516784
35	20.888186870145574	25.768229151061995	30.85020834856349	22.493375630228936
36	21.66105997106969	26.19966707373634	30.836905679281035	21.30236727591294
37	20.897654535670927	27.137445336215976	31.107153600289543	20.85774652782355
38	21.038710767611953	27.20719446704833	30.153675791179253	21.600418974160462
39	20.617938949137063	26.06400381582874	30.655701751757807	22.662355483276386
40	21.770956797484235	26.023017213174676	30.493912530754937	21.712113458586156
41	20.26811470016862	25.706030183876443	30.406786039148198	23.61906907680674
42	20.719566548700353	25.842292661121093	30.117482943221567	23.320657846956987
43	20.459505356421655	25.843491099795184	30.775665463034755	22.921338080748402
44	20.79315068328981	26.29410404125505	29.723556151046417	23.18918912440872
45	20.771099411686457	26.09756009870341	29.474041219099757	23.65729927051038
46	20.70698294262235	26.797568128242528	29.64937279711991	22.84607613201521
47	20.45770769841051	26.09156790533293	31.25575999587737	22.194964400379185
48	20.064979344909453	25.70447221360012	30.981796914979164	23.24875152651126
49	20.675104473891416	25.261768967389287	30.92906561331897	23.134060945400332
50	19.557440566430053	25.459631192482433	31.50779164903963	23.47513659204788
51	19.95951674158906	25.030829834891104	30.322775488094113	24.686877935425727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	466.0
1	992.0
2	1518.0
3	7406.0
4	13294.0
5	9233.5
6	5173.0
7	4502.5
8	3832.0
9	3717.5
10	3603.0
11	3477.0
12	3351.0
13	3325.5
14	3300.0
15	3222.0
16	3144.0
17	3147.5
18	3151.0
19	3189.5
20	3228.0
21	3493.0
22	3758.0
23	4063.0
24	4368.0
25	5476.5
26	8075.0
27	9565.0
28	11866.0
29	14167.0
30	16601.0
31	19035.0
32	21288.0
33	23541.0
34	25630.0
35	27719.0
36	30927.5
37	34136.0
38	37542.0
39	40948.0
40	44659.5
41	48371.0
42	52213.5
43	56056.0
44	59057.0
45	62058.0
46	65580.0
47	69102.0
48	69059.0
49	69016.0
50	65670.0
51	62324.0
52	56323.0
53	50322.0
54	45331.0
55	40340.0
56	37960.5
57	35581.0
58	33376.5
59	31172.0
60	28469.0
61	25766.0
62	22781.0
63	19796.0
64	16837.5
65	13879.0
66	11327.5
67	8776.0
68	7195.0
69	5614.0
70	4517.0
71	3420.0
72	2750.0
73	2080.0
74	1696.0
75	998.5
76	685.0
77	527.0
78	369.0
79	286.0
80	203.0
81	165.5
82	128.0
83	100.0
84	72.0
85	50.5
86	29.0
87	23.0
88	17.0
89	19.5
90	22.0
91	18.5
92	15.0
93	12.0
94	9.0
95	5.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	834419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.58573971322611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.78494307960237	30.757879740103984
2	7.5379697915602435	6.118681598528506
3	3.2060553567644035	3.9036038464750304
4	2.05742424632737	3.340083397644922
5	1.4844592447315086	3.0123938260782612
6	1.2013929345514365	2.9255652561008096
7	1.0310157396545077	2.92911755149
8	0.9200752722474097	2.9873548412807214
9	0.822335432562748	3.0037582640659406
>10	5.917199192866438	38.380230110832045
>50	0.024537900868450938	0.6614797709534004
>100	0.011300340748830632	0.8657674776043949
>500	6.457337570754521E-4	0.19105327415568044
>1k	6.457337570754521E-4	0.9230310446862915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4531	0.5430125632326205	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2513	0.30116763880017117	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	953	0.11421120564129052	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06100052851145528	0.0
2	0.0	0.0	0.0	0.2579040026653276	0.0
3	0.0	0.0	0.0	0.36025066543307377	0.0
4	0.0	0.0	0.0	0.46259732820081995	0.0
5	0.0	0.0	0.0	0.7723937254544779	0.0
6	0.0	0.0	0.0	1.099807171217338	0.0
7	0.0	0.0	0.0	1.391147612889927	0.0
8	0.0	0.0	0.0	2.0342298054095127	0.0
9	0.0	0.0	0.0	2.2631315921617317	0.0
10	0.0	0.0	0.0	2.796676489868999	0.0
11	0.0	0.0	0.0	3.449945411118395	0.0
12	0.0	0.0	0.0	3.9596413792111638	0.0
13	0.0	0.0	0.0	4.1777572178965245	0.0
14	0.0	0.0	0.0	4.2822610702776425	0.0
15	0.0	0.0	0.0	4.463225310066046	0.0
16	0.0	0.0	0.0	4.829228481134778	0.0
17	0.0	0.0	0.0	5.281998612208016	0.0
18	0.0	0.0	0.0	5.7858222307977165	0.0
19	0.0	0.0	0.0	6.0953789403165555	0.0
20	0.0	0.0	0.0	6.4112873748081	0.0
21	0.0	0.0	0.0	6.816599334387161	0.0
22	0.0	0.0	0.0	7.253669918829749	0.0
23	0.0	0.0	0.0	7.73160726205899	0.0
24	0.0	0.0	0.0	8.091857927492063	0.0
25	0.0	0.0	0.0	8.441921864195326	0.0
26	0.0	0.0	0.0	8.745486380343689	0.0
27	0.0	0.0	0.0	9.053005744116565	0.0
28	0.0	0.0	0.0	9.365318862585823	0.0
29	0.0	0.0	0.0	9.705435758294094	0.0
30	0.0	0.0	0.0	10.092052074557266	0.0
31	1.1984386740953885E-4	0.0	0.0	10.452662271592569	0.0
32	1.1984386740953885E-4	0.0	0.0	10.794097449842345	0.0
33	1.1984386740953885E-4	0.0	0.0	11.147277327098257	0.0
34	1.1984386740953885E-4	0.0	0.0	11.485117189325747	0.0
35	1.1984386740953885E-4	0.0	0.0	11.845847230228458	0.0
36	1.1984386740953885E-4	0.0	0.0	12.189439598091607	0.0
37	1.1984386740953885E-4	0.0	0.0	12.556761051701843	0.0
38	1.1984386740953885E-4	0.0	0.0	12.932112044428518	0.0
39	1.1984386740953885E-4	0.0	0.0	13.355760115721239	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTGCG	20	7.0321735E-4	45.000004	45
TCGACGA	20	7.0321735E-4	45.000004	1
CTAGCGT	20	7.0321735E-4	45.000004	44
CGTTTTA	1175	0.0	42.89362	1
CGTTATT	510	0.0	41.911766	1
CGTTTTT	2025	0.0	41.77778	1
ACGTTAG	60	3.6379788E-12	41.250004	2
TCACGAC	45	1.9272193E-8	40.0	25
GCGACGC	35	6.2462314E-6	38.571426	19
CGTATAG	35	6.2462314E-6	38.571426	2
TCGATAG	35	6.2462314E-6	38.571426	2
AGGCGAT	365	0.0	37.60274	7
CAACCCG	30	1.1396535E-4	37.500004	13
CGAGACA	30	1.1396535E-4	37.500004	22
ACCCGGT	30	1.1396535E-4	37.500004	15
ACGGTTC	30	1.1396535E-4	37.500004	38
TATGCGG	85	0.0	37.058823	2
AATCCGT	25	0.0021067783	36.0	20
CCGTATG	25	0.0021067783	36.0	39
GCCGATC	25	0.0021067783	36.0	16
>>END_MODULE