FastQCFastQC Report
Sat 14 Jan 2017
SRR2934596.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934596.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592218
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15740.26578050650267304No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.1526464916635428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGCG406.8048394E-945.020
CGTATAG253.8885075E-545.02
GTCGAAC253.8885075E-545.08
AATAGGT302.1637134E-644.9999964
TAGTGCG302.1637134E-644.9999961
TCGACGG302.1637134E-644.9999962
CGTTTAG302.1637134E-644.9999962
AATAGGC750.042.04
CGTTTTA3100.040.6451641
CGACCCG403.4544428E-739.37532
CACGACG403.4544428E-739.37526
TTTAGCG356.243161E-638.5714261
CGCATAG301.1392537E-437.4999962
CGCGAAG301.1392537E-437.4999962
CGTTATT1200.037.4999961
AGGCGAT2250.037.0000047
GTTACGG552.744855E-936.818182
AGCGTCG250.002106285736.01
TCTTCGT250.002106285736.038
CCCGTCA250.002106285736.037
Produced by FastQC (version 0.11.5)