##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934596.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592218
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.923416714790836	31.0	31.0	34.0	30.0	34.0
2	32.12284496587405	33.0	31.0	34.0	30.0	34.0
3	32.02282436535195	33.0	31.0	34.0	30.0	34.0
4	35.78379245480549	37.0	35.0	37.0	35.0	37.0
5	32.066428240951815	37.0	35.0	37.0	0.0	37.0
6	33.920927766464374	37.0	35.0	37.0	19.0	37.0
7	20.778660898520478	32.0	0.0	37.0	0.0	37.0
8	28.101396782941418	35.0	17.0	37.0	17.0	37.0
9	34.83505398349932	37.0	32.0	39.0	32.0	39.0
10	36.17353575879152	37.0	35.0	39.0	32.0	39.0
11	36.587378296505676	38.0	35.0	39.0	32.0	39.0
12	36.883946452151065	39.0	35.0	39.0	33.0	39.0
13	36.54885700873665	39.0	35.0	39.0	32.0	39.0
14	37.67938326764806	40.0	37.0	41.0	33.0	41.0
15	37.934716607735666	40.0	37.0	41.0	33.0	41.0
16	38.090868565291835	40.0	37.0	41.0	33.0	41.0
17	38.10536153916294	40.0	37.0	41.0	33.0	41.0
18	38.027763424955	40.0	37.0	41.0	33.0	41.0
19	37.976969629427	40.0	37.0	41.0	33.0	41.0
20	37.946850652969005	40.0	37.0	41.0	33.0	41.0
21	37.864619109854814	40.0	36.0	41.0	33.0	41.0
22	37.89622740274696	40.0	36.0	41.0	33.0	41.0
23	37.88379110395159	40.0	36.0	41.0	33.0	41.0
24	37.7500464356031	40.0	36.0	41.0	33.0	41.0
25	37.62344103016119	39.0	36.0	41.0	33.0	41.0
26	37.488073648555094	39.0	35.0	41.0	33.0	41.0
27	37.44950339233188	39.0	35.0	41.0	33.0	41.0
28	37.362228098436724	39.0	35.0	41.0	33.0	41.0
29	37.32970460202155	39.0	35.0	41.0	32.0	41.0
30	37.135598715337935	39.0	35.0	41.0	32.0	41.0
31	37.00377394810695	39.0	35.0	41.0	31.0	41.0
32	36.919764343535654	39.0	35.0	41.0	31.0	41.0
33	36.8044436339321	39.0	35.0	41.0	31.0	41.0
34	36.62510595760345	39.0	35.0	41.0	30.0	41.0
35	36.466794322361025	39.0	35.0	41.0	30.0	41.0
36	36.32445484601954	39.0	35.0	40.0	30.0	41.0
37	36.22072277438376	39.0	35.0	40.0	30.0	41.0
38	36.02357071213641	39.0	35.0	40.0	29.0	41.0
39	35.86560861034281	39.0	35.0	40.0	28.0	41.0
40	35.74949427406799	39.0	35.0	40.0	27.0	41.0
41	35.602816868112754	39.0	35.0	40.0	27.0	41.0
42	35.52328534424823	38.0	35.0	40.0	26.0	41.0
43	35.45011971942764	38.0	34.0	40.0	26.0	41.0
44	35.31452438122448	38.0	34.0	40.0	26.0	41.0
45	35.223865198288465	38.0	34.0	40.0	26.0	41.0
46	35.144593713801335	38.0	34.0	40.0	25.0	41.0
47	35.0402909063892	38.0	34.0	40.0	24.0	41.0
48	34.88715304161643	38.0	34.0	40.0	24.0	41.0
49	34.80419710309379	38.0	34.0	40.0	24.0	41.0
50	34.62181831690357	37.0	34.0	40.0	24.0	41.0
51	32.69345916537491	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	26.0
9	24.0
10	31.0
11	20.0
12	13.0
13	10.0
14	17.0
15	31.0
16	67.0
17	123.0
18	243.0
19	442.0
20	723.0
21	1271.0
22	1941.0
23	2971.0
24	4327.0
25	5920.0
26	7565.0
27	8623.0
28	9660.0
29	11137.0
30	13564.0
31	17240.0
32	22699.0
33	31949.0
34	42666.0
35	53102.0
36	67797.0
37	110294.0
38	108736.0
39	68977.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.56870611835506	21.431128402041143	26.318348986352998	12.681816493250796
2	30.89217146388661	24.56510946982361	27.29214579766235	17.25057326862743
3	31.64071338594909	23.365382342313133	28.286880844553863	16.70702342718391
4	28.36573694146412	25.36852983192	27.75481326133282	18.510919965283055
5	23.16494939363545	36.14800630848775	23.78380934047935	16.903234957397444
6	26.38150140657663	34.0280099557933	25.541607988950016	14.048880648680045
7	49.89243825753354	42.773607016335205	4.832848714493649	2.5011060116376065
8	86.8636211665299	3.7553738657048585	6.647552083861011	2.733452883904238
9	81.79623044216827	5.707357763526269	8.203060359529768	4.293351434775708
10	43.53819032856144	25.623334650415895	16.132066232367134	14.706408788655528
11	33.32421506945077	25.106970743881472	23.909945324188055	17.658868862479697
12	29.852351667798004	22.998963219625207	27.5417160572627	19.606969055314092
13	26.173132191186355	23.442887585314867	30.525752341198682	19.8582278823001
14	21.9873424988771	24.99113502122529	31.974712014832374	21.04681046506523
15	20.6314228881932	24.456872300402893	34.463322627816105	20.448382183587803
16	26.25215714483518	23.800357300858803	29.757623037462555	20.18986251684346
17	27.543235767909792	22.95354075695099	27.449182564528602	22.05404091061062
18	27.167698381339306	23.751051133197574	28.903039083580705	20.178211401882415
19	26.00005403415634	24.96546879696328	27.404773242285778	21.629703926594598
20	25.745924642614714	27.259049876903436	27.49257874634003	19.50244673414182
21	25.631102060389928	24.49621592048874	30.23987788280667	19.632804136314668
22	24.647173844766623	22.69198166891246	30.751513800661247	21.909330685659672
23	23.583545248540233	24.003660814092108	30.75202037087694	21.660773566490718
24	23.06414192071163	25.084681654390785	30.077775413783435	21.773401011114153
25	24.131316508447902	25.1093347382214	28.26628032244883	22.49306843088187
26	21.756853050734694	26.499532266834176	27.713612217122748	24.030002465308385
27	21.741149374048067	25.576392477094583	30.784103151204455	21.89835499765289
28	20.49616864060194	25.95885298994627	30.03150866741639	23.5134697020354
29	22.1778128999794	26.231387765991578	29.62051136574707	21.97028796828195
30	24.235669972881606	24.914305205177822	29.61071767491025	21.239307147030317
31	24.934061443590032	24.28902870226842	29.110564015278158	21.66634583886339
32	25.6777065202341	24.497904487874397	27.681698293533803	22.1426906983577
33	25.260968089453545	24.66844979382592	28.298700816253476	21.771881300467058
34	23.13489289417073	24.173361836350804	30.27550665464407	22.416238614834402
35	22.65736603750646	26.249455437018128	29.026473359472355	22.066705166003057
36	25.210479924622355	25.68800678128662	27.816783684386493	21.284729609704534
37	22.833990186046353	27.101506539821486	28.402041140255786	21.662462133876375
38	24.05381126544617	26.0655704487199	27.550496607668123	22.33012167816581
39	23.402024254581928	25.243406988642697	27.580046536917145	23.774522219858227
40	25.22044247219774	24.640926145439686	27.638133254983803	22.50049812737877
41	22.069069160342984	24.245632520456994	27.944945948957983	25.740352370242036
42	21.96370255547788	24.495540493534477	29.48255541033876	24.05820154064888
43	21.964715695909277	24.6101942190207	29.689911485297642	23.73517859977238
44	23.184874488786225	24.73768105663793	28.591667257665254	23.485777196910597
45	22.284699215491592	24.6166107750862	27.55269174526948	25.545998264152725
46	22.553350286549886	24.892691542641394	28.351890688901722	24.202067481907
47	21.73422624776687	25.197477955752777	30.026780678736547	23.041515117743806
48	21.466588317139973	26.271913383247387	29.440003512220166	22.821494787392478
49	23.531875086539078	24.16525671289964	29.53118615104573	22.771682049515547
50	22.239276752817375	25.24593983972118	28.82570269731754	23.6890807101439
51	21.876910191855025	26.142400264767367	27.256010455609253	24.724679087768354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	365.0
1	899.0
2	1433.0
3	3082.0
4	4731.0
5	3279.0
6	1827.0
7	1661.5
8	1496.0
9	1531.0
10	1566.0
11	1538.0
12	1510.0
13	1489.0
14	1468.0
15	1412.0
16	1356.0
17	1474.5
18	1593.0
19	1507.5
20	1422.0
21	1648.0
22	1874.0
23	2292.0
24	2710.0
25	3114.0
26	4413.5
27	5309.0
28	6281.0
29	7253.0
30	8396.5
31	9540.0
32	11409.5
33	13279.0
34	14593.0
35	15907.0
36	17709.5
37	19512.0
38	21909.0
39	24306.0
40	26956.0
41	29606.0
42	31915.5
43	34225.0
44	36417.5
45	38610.0
46	41721.5
47	44833.0
48	46051.5
49	47270.0
50	46540.0
51	45810.0
52	42448.5
53	39087.0
54	37865.0
55	36643.0
56	35953.0
57	35263.0
58	33372.0
59	31481.0
60	28558.5
61	25636.0
62	22743.0
63	19850.0
64	17525.5
65	15201.0
66	12871.5
67	10542.0
68	8550.0
69	6558.0
70	5315.5
71	4073.0
72	3291.0
73	2509.0
74	1990.0
75	1097.5
76	724.0
77	573.5
78	423.0
79	305.5
80	188.0
81	149.5
82	111.0
83	89.5
84	68.0
85	49.5
86	31.0
87	23.0
88	15.0
89	10.5
90	6.0
91	6.5
92	7.0
93	3.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	592218.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.50971833080961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.78583939876552	28.05195770980859
2	7.1613350273082546	5.372393194937868
3	3.159909218922345	3.5558191425811727
4	2.1246964531951673	3.1878706199128373
5	1.500867387640393	2.8148556481144595
6	1.1761234623931736	2.6469635877974693
7	1.026322791434018	2.69479551762262
8	0.9173177921887059	2.7526665603870804
9	0.8736549072842553	2.94934945415058
>10	7.229862014072073	44.00848769727513
>50	0.03147967628299425	0.789860215564721
>100	0.011623265088990089	0.7248257267890333
>500	4.8430271204125364E-4	0.16422116717225219
>1k	4.8430271204125364E-4	0.2859337578862001
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1574	0.26578050650267304	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	904	0.1526464916635428	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.032758207281777994	0.0
2	0.0	0.0	0.0	0.15365963209493802	0.0
3	1.6885673856586596E-4	0.0	0.0	0.2063429345274882	0.0
4	1.6885673856586596E-4	0.0	0.0	0.27743162146371775	0.0
5	1.6885673856586596E-4	0.0	0.0	0.5167016200115498	0.0
6	1.6885673856586596E-4	0.0	0.0	0.6728941031849758	0.0
7	1.6885673856586596E-4	0.0	0.0	0.8528953864961889	0.0
8	1.6885673856586596E-4	0.0	0.0	1.2346804723936118	0.0
9	1.6885673856586596E-4	0.0	0.0	1.378039843436032	0.0
10	1.6885673856586596E-4	0.0	0.0	1.836485888642358	0.0
11	1.6885673856586596E-4	0.0	0.0	2.3536940788696055	0.0
12	1.6885673856586596E-4	0.0	0.0	2.7994758686834915	0.0
13	1.6885673856586596E-4	0.0	0.0	2.966306326386567	0.0
14	1.6885673856586596E-4	0.0	0.0	3.0417852885255092	0.0
15	1.6885673856586596E-4	0.0	0.0	3.1788969602409924	0.0
16	1.6885673856586596E-4	0.0	0.0	3.4456906071750604	1.6885673856586596E-4
17	1.6885673856586596E-4	0.0	0.0	3.768713548051562	1.6885673856586596E-4
18	1.6885673856586596E-4	0.0	0.0	4.132937533138135	1.6885673856586596E-4
19	1.6885673856586596E-4	0.0	0.0	4.37372724233306	1.6885673856586596E-4
20	1.6885673856586596E-4	0.0	0.0	4.635286330371586	1.6885673856586596E-4
21	1.6885673856586596E-4	0.0	0.0	4.9630372599279315	1.6885673856586596E-4
22	1.6885673856586596E-4	0.0	0.0	5.3046344420466784	1.6885673856586596E-4
23	1.6885673856586596E-4	0.0	0.0	5.6337362255115515	1.6885673856586596E-4
24	1.6885673856586596E-4	0.0	0.0	5.8898918979159705	1.6885673856586596E-4
25	1.6885673856586596E-4	0.0	0.0	6.153308410078721	1.6885673856586596E-4
26	1.6885673856586596E-4	0.0	0.0	6.394604689489343	1.6885673856586596E-4
27	1.6885673856586596E-4	0.0	0.0	6.696351681306546	1.6885673856586596E-4
28	1.6885673856586596E-4	0.0	0.0	6.95064992958674	1.6885673856586596E-4
29	1.6885673856586596E-4	0.0	0.0	7.248344359678362	1.6885673856586596E-4
30	1.6885673856586596E-4	0.0	0.0	7.590110398535674	1.6885673856586596E-4
31	1.6885673856586596E-4	0.0	0.0	7.8800374186532665	1.6885673856586596E-4
32	1.6885673856586596E-4	0.0	0.0	8.152065624482876	1.6885673856586596E-4
33	1.6885673856586596E-4	0.0	0.0	8.42679553812954	1.6885673856586596E-4
34	1.6885673856586596E-4	0.0	0.0	8.73884279099926	1.6885673856586596E-4
35	1.6885673856586596E-4	0.0	0.0	9.054267178640298	1.6885673856586596E-4
36	1.6885673856586596E-4	0.0	0.0	9.340648207248007	1.6885673856586596E-4
37	1.6885673856586596E-4	0.0	0.0	9.640537774940984	1.6885673856586596E-4
38	1.6885673856586596E-4	0.0	0.0	10.006956897628914	1.6885673856586596E-4
39	1.6885673856586596E-4	0.0	0.0	10.661276759571644	1.6885673856586596E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGCG	40	6.8048394E-9	45.0	20
CGTATAG	25	3.8885075E-5	45.0	2
GTCGAAC	25	3.8885075E-5	45.0	8
AATAGGT	30	2.1637134E-6	44.999996	4
TAGTGCG	30	2.1637134E-6	44.999996	1
TCGACGG	30	2.1637134E-6	44.999996	2
CGTTTAG	30	2.1637134E-6	44.999996	2
AATAGGC	75	0.0	42.0	4
CGTTTTA	310	0.0	40.645164	1
CGACCCG	40	3.4544428E-7	39.375	32
CACGACG	40	3.4544428E-7	39.375	26
TTTAGCG	35	6.243161E-6	38.571426	1
CGCATAG	30	1.1392537E-4	37.499996	2
CGCGAAG	30	1.1392537E-4	37.499996	2
CGTTATT	120	0.0	37.499996	1
AGGCGAT	225	0.0	37.000004	7
GTTACGG	55	2.744855E-9	36.81818	2
AGCGTCG	25	0.0021062857	36.0	1
TCTTCGT	25	0.0021062857	36.0	38
CCCGTCA	25	0.0021062857	36.0	37
>>END_MODULE