##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934595.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	462846
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.902941799216155	31.0	31.0	34.0	30.0	34.0
2	32.09284513639526	33.0	31.0	34.0	30.0	34.0
3	31.95701594050721	33.0	31.0	34.0	30.0	34.0
4	35.74944365944612	37.0	35.0	37.0	35.0	37.0
5	32.10321143533702	37.0	35.0	37.0	10.0	37.0
6	33.957193105266114	37.0	35.0	37.0	19.0	37.0
7	20.621595519892146	32.0	0.0	37.0	0.0	37.0
8	28.016899789562835	35.0	17.0	37.0	17.0	37.0
9	34.751902360612384	37.0	32.0	39.0	31.0	39.0
10	36.15101783314537	37.0	35.0	39.0	32.0	39.0
11	36.62952040203437	38.0	35.0	39.0	33.0	39.0
12	36.99511500585508	39.0	37.0	39.0	33.0	39.0
13	36.774426050997526	39.0	37.0	39.0	33.0	39.0
14	38.07237396455841	40.0	37.0	41.0	33.0	41.0
15	38.26665240706412	40.0	38.0	41.0	34.0	41.0
16	38.346830263197695	40.0	38.0	41.0	34.0	41.0
17	38.33301789364065	40.0	37.0	41.0	34.0	41.0
18	38.23516245144173	40.0	37.0	41.0	34.0	41.0
19	38.18470722443318	40.0	37.0	41.0	34.0	41.0
20	38.1687969648652	40.0	37.0	41.0	34.0	41.0
21	38.06366264373031	40.0	37.0	41.0	34.0	41.0
22	38.08588169715197	40.0	37.0	41.0	34.0	41.0
23	38.0183884056468	40.0	37.0	41.0	34.0	41.0
24	37.91450071946176	40.0	36.0	41.0	33.0	41.0
25	37.78017526347857	40.0	36.0	41.0	33.0	41.0
26	37.660435652463235	40.0	36.0	41.0	33.0	41.0
27	37.610632910298456	40.0	36.0	41.0	33.0	41.0
28	37.540343872475944	40.0	36.0	41.0	33.0	41.0
29	37.538068817706105	40.0	36.0	41.0	33.0	41.0
30	37.39298600398405	39.0	36.0	41.0	33.0	41.0
31	37.27370442868686	39.0	36.0	41.0	32.0	41.0
32	37.17707185543356	39.0	35.0	41.0	32.0	41.0
33	37.027683073851776	39.0	35.0	41.0	31.0	41.0
34	36.8438551915756	39.0	35.0	41.0	31.0	41.0
35	36.68605972612921	39.0	35.0	41.0	30.0	41.0
36	36.49976666104925	39.0	35.0	41.0	30.0	41.0
37	36.404717767896884	39.0	35.0	41.0	30.0	41.0
38	36.25005725446477	39.0	35.0	40.0	30.0	41.0
39	36.16731915151044	39.0	35.0	40.0	29.0	41.0
40	36.010299322020714	39.0	35.0	40.0	28.0	41.0
41	35.86360474110179	39.0	35.0	40.0	27.0	41.0
42	35.70777753291591	39.0	35.0	40.0	27.0	41.0
43	35.60532660971468	39.0	35.0	40.0	26.0	41.0
44	35.448079922911724	38.0	35.0	40.0	26.0	41.0
45	35.37233766738829	38.0	34.0	40.0	25.0	41.0
46	35.18835854690329	38.0	34.0	40.0	24.0	41.0
47	35.11744511133293	38.0	34.0	40.0	24.0	41.0
48	34.96621338414937	38.0	34.0	40.0	24.0	41.0
49	34.79983190953362	38.0	34.0	40.0	23.0	41.0
50	34.61572099575237	38.0	34.0	40.0	23.0	41.0
51	32.62728423708966	36.0	31.0	39.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	24.0
10	28.0
11	15.0
12	12.0
13	10.0
14	16.0
15	23.0
16	44.0
17	79.0
18	142.0
19	240.0
20	461.0
21	781.0
22	1378.0
23	2132.0
24	3115.0
25	4455.0
26	5583.0
27	6494.0
28	7296.0
29	8300.0
30	10172.0
31	12799.0
32	16928.0
33	23389.0
34	31758.0
35	41007.0
36	53592.0
37	87004.0
38	88302.0
39	57248.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.70943683212126	19.55963754682983	25.89306162308846	13.837863997960445
2	30.62465701334785	24.185798300082535	26.727032317444678	18.462512369124937
3	29.938035545300163	23.17098991889311	29.67272051611119	17.218254019695536
4	26.43838339318045	25.216162611322122	28.74908716938247	19.59636682611495
5	21.55274108450759	36.29068847953747	24.778652078661153	17.377918357293787
6	24.9942745535232	34.63398192919459	25.591449423782425	14.780294093499782
7	48.40292451484943	43.34638303020875	5.495348344805832	2.755344110135985
8	83.55478928196419	4.268590416682871	8.422023740077693	3.7545965612752403
9	77.25139679288576	7.188352065265768	10.635502953466164	4.9247481883823125
10	37.369232963015776	31.55455594301344	17.275292429879485	13.800918664091297
11	29.53314925482774	24.48028069811557	27.87298583114038	18.11358421591631
12	27.505044874537106	22.61810623836006	30.525920068446094	19.350928818656747
13	23.31315383518492	24.756614511090085	32.57541385255571	19.35481780116929
14	19.708931264394636	27.075744416069274	32.06487687049256	21.150447449043526
15	19.38398517001335	25.796053114858935	34.81870859854034	20.001253116587375
16	22.69955881653941	25.195421371255232	32.19623805758287	19.90878175462249
17	23.022992528832482	25.23474330554871	29.57830466289003	22.16395950272877
18	23.127130838335	25.10532660971468	31.30695738971494	20.460585162235386
19	22.431219023174016	26.78731154638908	29.113571252641268	21.66789817779564
20	23.810511487622232	27.125653024980227	29.47697506297991	19.586860424417623
21	23.03617185845832	26.7594405050492	31.241924959921874	18.96246267657061
22	22.84280300575137	23.969959770636454	30.811760283117927	22.375476940494245
23	20.67577552792938	25.89932720602533	31.358378380714104	22.06651888533119
24	21.25653024980231	25.034028596984744	31.949935831788544	21.759505321424406
25	21.038099065347872	26.897283329660404	29.65543614938878	22.409181455602944
26	20.69046723964342	27.10296729365707	29.956616239526756	22.249949227172753
27	20.15270737999248	26.517675425519506	31.853143378143056	21.476473816344964
28	18.406338177277107	26.530422645977282	31.48973956780441	23.573499608941205
29	19.80161868094355	24.783405279509815	31.633199811600406	23.781776227946228
30	20.94346715754268	25.57891825790868	30.8847867325201	22.592827852028535
31	21.41900329699295	25.478668930918708	29.85139765710409	23.25093011498425
32	21.80790154824715	25.86173370840409	29.564693224096132	22.765671519252624
33	21.666169741123397	25.062979911244774	29.805161976121646	23.46568837151018
34	19.391114971286346	25.084585369647787	30.791019043051037	24.73328061601483
35	19.26774780380515	26.149086305164136	31.423194755923134	23.159971135107575
36	21.357211685960344	26.520700189695923	29.910164503960278	22.211923620383452
37	19.980943985688544	26.932716281441344	30.170942386884626	22.91539734598549
38	19.845909870669725	27.713321493542132	28.999062323105306	23.441706312682836
39	20.48413511189467	25.63487639517248	29.29786581281895	24.583122680113902
40	21.371471288506328	25.118289884756486	29.861984331721565	23.64825449501562
41	18.995302973343186	25.46030429127615	30.787994278874613	24.75639845650605
42	19.744364216175576	26.0175090634898	30.591168552823184	23.64695816751144
43	20.1112248998587	25.451013944162852	30.504098555459052	23.933662600519394
44	19.765321510826496	26.01988566391413	29.502685558479495	24.712107266779878
45	20.142552814543066	25.156531546129813	29.305427723260003	25.39548791606712
46	20.476573201453615	25.86389425424439	29.407837596090275	24.251694948211718
47	19.116941704152136	25.9529087428648	31.32921101187004	23.600938541113024
48	20.03193286751965	25.102950009290346	31.172139329280153	23.692977793909854
49	20.594755058918086	24.685748607528204	30.834446014441085	23.885050319112622
50	19.699856971865373	25.132765541886503	30.664195002225362	24.503182484022766
51	19.46025243817598	24.901803191558315	29.35857715093141	26.279367219334294
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	239.0
1	623.5
2	1008.0
3	2984.5
4	4961.0
5	3443.0
6	1925.0
7	1730.0
8	1535.0
9	1587.0
10	1639.0
11	1599.5
12	1560.0
13	1545.5
14	1531.0
15	1515.0
16	1499.0
17	1433.5
18	1368.0
19	1468.0
20	1568.0
21	1695.0
22	1822.0
23	2182.5
24	2543.0
25	3208.0
26	4676.0
27	5479.0
28	6425.0
29	7371.0
30	8651.5
31	9932.0
32	11490.0
33	13048.0
34	14442.0
35	15836.0
36	17127.0
37	18418.0
38	20287.5
39	22157.0
40	23927.0
41	25697.0
42	28088.5
43	30480.0
44	32699.0
45	34918.0
46	36916.5
47	38915.0
48	39277.5
49	39640.0
50	37786.0
51	35932.0
52	32256.0
53	28580.0
54	25946.5
55	23313.0
56	21830.0
57	20347.0
58	19077.5
59	17808.0
60	16176.5
61	14545.0
62	12736.0
63	10927.0
64	9423.0
65	7919.0
66	6586.0
67	5253.0
68	4511.5
69	3770.0
70	3029.5
71	2289.0
72	1815.5
73	1342.0
74	1091.5
75	644.0
76	447.0
77	372.5
78	298.0
79	225.5
80	153.0
81	111.0
82	69.0
83	46.5
84	24.0
85	18.0
86	12.0
87	9.5
88	7.0
89	5.5
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	462846.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.25924226830181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35603393172185	31.844888942990057
2	7.653004417721202	6.468203355377286
3	3.3671835797745278	4.268838799786186
4	2.25778977840786	3.8164994094653295
5	1.5902122287003975	3.3600581915333128
6	1.2790401529915922	3.2430760617694503
7	1.1028634598388367	3.2624321896730866
8	0.9723770964089473	3.2873535434634857
9	0.8715172430926301	3.314669248516855
>10	5.52174796936581	35.4441399343153
>50	0.02038843587178905	0.5551795962838074
>100	0.006796145290595771	0.560039903135437
>500	5.227804069689054E-4	0.2127488862798524
>1k	5.227804069689054E-4	0.36187193741059004
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1638	0.35389740864131913	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	963	0.20806056442099533	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07324250398620707	0.0
2	0.0	0.0	0.0	0.35411346322534926	0.0
3	0.0	0.0	0.0	0.4688384473453373	0.0
4	0.0	0.0	0.0	0.5930698331626502	0.0
5	0.0	0.0	0.0	0.9934189773704428	0.0
6	0.0	0.0	0.0	1.3827493377927	0.0
7	0.0	0.0	0.0	1.7552274406606085	0.0
8	0.0	0.0	0.0	2.682965824485898	0.0
9	0.0	0.0	0.0	3.030597650190344	0.0
10	0.0	0.0	0.0	3.7878689672158776	0.0
11	0.0	0.0	0.0	4.783448490426621	0.0
12	0.0	0.0	0.0	5.533590006179161	0.0
13	0.0	0.0	0.0	5.856159500136115	0.0
14	0.0	0.0	0.0	6.00480505394883	0.0
15	0.0	0.0	0.0	6.231662367180444	0.0
16	0.0	0.0	0.0	6.700932923693842	0.0
17	0.0	0.0	0.0	7.263323005924216	0.0
18	0.0	0.0	0.0	7.844293782381181	0.0
19	0.0	0.0	0.0	8.197975136438469	0.0
20	0.0	0.0	0.0	8.55230465424785	0.0
21	0.0	0.0	0.0	8.996512879013753	0.0
22	0.0	0.0	0.0	9.475289837224476	0.0
23	0.0	0.0	0.0	9.981937836775083	0.0
24	0.0	0.0	0.0	10.385095690575266	0.0
25	0.0	0.0	0.0	10.756493520523025	0.0
26	0.0	0.0	0.0	11.116440457517188	0.0
27	0.0	0.0	0.0	11.499289180418542	0.0
28	0.0	0.0	0.0	11.860964554084944	0.0
29	0.0	0.0	0.0	12.256776552028104	0.0
30	0.0	0.0	0.0	12.66533577042904	0.0
31	0.0	0.0	0.0	13.028307471599625	0.0
32	0.0	0.0	0.0	13.39365577319454	0.0
33	0.0	0.0	0.0	13.736966507218384	0.0
34	0.0	0.0	0.0	14.077684586233866	0.0
35	0.0	0.0	0.0	14.48041033086599	0.0
36	0.0	0.0	0.0	14.830634811578797	0.0
37	0.0	0.0	0.0	15.211971152391941	0.0
38	0.0	0.0	0.0	15.577535508570886	0.0
39	0.0	0.0	0.0	16.034274899210537	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	20	7.028913E-4	45.000004	22
TACGCGG	20	7.028913E-4	45.000004	2
TGCGAAA	20	7.028913E-4	45.000004	1
GGACGTA	20	7.028913E-4	45.000004	8
TAGGCCG	20	7.028913E-4	45.000004	6
TCACGAC	45	3.8380676E-10	45.0	25
GTTAGCG	25	3.887183E-5	45.0	1
GCCGACC	25	3.887183E-5	45.0	14
GCGTAAA	35	1.209828E-7	45.0	1
CACGACC	30	2.162682E-6	44.999996	27
TAAGGCG	30	2.162682E-6	44.999996	5
CGAAGGA	95	0.0	42.63158	4
CGTTTTA	395	0.0	41.582275	1
AGTACGG	65	0.0	41.53846	2
TTAATCG	55	6.002665E-11	40.909092	20
CTCACGA	50	1.0786607E-9	40.5	24
CGTTGAT	45	1.9239451E-8	40.0	25
ATAGCGG	45	1.9239451E-8	40.0	2
TACGGGT	45	1.9239451E-8	40.0	4
CGGTCTA	45	1.9239451E-8	40.0	31
>>END_MODULE