##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934594.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	989418
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.892824872804013	31.0	31.0	34.0	30.0	34.0
2	32.10137777966441	33.0	31.0	34.0	30.0	34.0
3	31.704038131507613	33.0	31.0	34.0	30.0	34.0
4	35.62685538367	37.0	35.0	37.0	33.0	37.0
5	31.984721321019023	37.0	35.0	37.0	0.0	37.0
6	33.89709303853376	37.0	35.0	37.0	19.0	37.0
7	20.844883557808732	32.0	0.0	37.0	0.0	37.0
8	28.143950281882884	35.0	17.0	37.0	17.0	37.0
9	34.85704929564653	37.0	32.0	39.0	32.0	39.0
10	36.30953550471085	37.0	35.0	39.0	32.0	39.0
11	36.734793585724134	38.0	37.0	39.0	33.0	39.0
12	37.05184360907119	39.0	37.0	39.0	34.0	39.0
13	36.815322745290665	39.0	37.0	39.0	33.0	39.0
14	38.04304247547548	40.0	37.0	41.0	33.0	41.0
15	38.238814131135676	40.0	38.0	41.0	33.0	41.0
16	38.33417221032971	40.0	38.0	41.0	33.0	41.0
17	38.335759001756585	40.0	38.0	41.0	34.0	41.0
18	38.20515393898231	40.0	37.0	41.0	34.0	41.0
19	38.10751674216559	40.0	37.0	41.0	34.0	41.0
20	38.018784780547755	40.0	36.0	41.0	34.0	41.0
21	37.92085953560578	40.0	36.0	41.0	33.0	41.0
22	37.88467866968258	39.0	36.0	41.0	33.0	41.0
23	37.81859032279583	39.0	36.0	41.0	33.0	41.0
24	37.672267939334034	39.0	35.0	41.0	33.0	41.0
25	37.52128119763336	39.0	35.0	41.0	33.0	41.0
26	37.39266012949027	39.0	35.0	41.0	33.0	41.0
27	37.34286418884637	39.0	35.0	41.0	33.0	41.0
28	37.21771283724371	39.0	35.0	41.0	33.0	41.0
29	37.157191399388324	39.0	35.0	41.0	33.0	41.0
30	36.91116191538864	39.0	35.0	41.0	32.0	41.0
31	36.679484302893215	39.0	35.0	41.0	31.0	41.0
32	36.53145384458338	39.0	35.0	41.0	30.0	41.0
33	36.237143452009164	39.0	35.0	41.0	30.0	41.0
34	35.90585374432242	39.0	35.0	40.0	28.0	41.0
35	35.612353929279635	39.0	35.0	40.0	25.0	41.0
36	35.339141798511854	39.0	34.0	40.0	24.0	41.0
37	35.233444307663696	38.0	34.0	40.0	24.0	41.0
38	35.04801610643833	38.0	34.0	40.0	23.0	41.0
39	34.87494971791497	38.0	34.0	40.0	23.0	41.0
40	34.742696211308065	38.0	34.0	40.0	22.0	41.0
41	34.56276619184207	38.0	34.0	40.0	21.0	41.0
42	34.44058426266755	38.0	33.0	40.0	20.0	41.0
43	34.361214370468296	38.0	33.0	40.0	20.0	41.0
44	34.17794299274927	38.0	33.0	40.0	19.0	41.0
45	34.077710330719675	38.0	33.0	40.0	20.0	41.0
46	33.97455372754488	38.0	33.0	40.0	19.0	41.0
47	33.87814957884332	38.0	33.0	40.0	18.0	41.0
48	33.733085510876094	37.0	33.0	40.0	18.0	41.0
49	33.60601889191424	37.0	33.0	40.0	18.0	41.0
50	33.450930749187904	37.0	33.0	40.0	18.0	41.0
51	31.61234483302305	35.0	29.0	39.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	28.0
9	56.0
10	60.0
11	36.0
12	32.0
13	29.0
14	38.0
15	55.0
16	118.0
17	225.0
18	400.0
19	783.0
20	1437.0
21	2577.0
22	4468.0
23	7313.0
24	11289.0
25	16108.0
26	19000.0
27	20225.0
28	20598.0
29	21648.0
30	24166.0
31	28983.0
32	37184.0
33	51800.0
34	67940.0
35	84519.0
36	107670.0
37	175187.0
38	174442.0
39	110984.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.02287607462165	20.544805127863047	24.523103481036326	17.90921531647898
2	36.66711137254426	22.37234414575033	24.827524868154814	16.13301961355059
3	30.505812507959224	22.502622753982646	31.2942558150347	15.697308923023435
4	27.379530188454222	23.622978356973494	31.129209292735734	17.868282161836554
5	21.703466078037795	35.445383043364885	26.577745705050848	16.273405173546468
6	25.60596229298436	32.11898307894136	28.72052054844363	13.55453407963065
7	46.91576259983142	42.398561578625014	8.084853924226161	2.6008218973174126
8	81.65557934058204	3.6828721531243622	11.874253348938467	2.787295157355132
9	75.27566710935115	6.735575863790633	13.630841565445545	4.357915461412669
10	39.209717227703564	28.448239267933268	19.94495754069564	12.39708596366753
11	30.75424138230758	24.085876747744635	28.672310388531443	16.487571481416346
12	28.533339801782464	21.5017313208371	31.522167577303023	18.44276130007742
13	25.00207192511153	21.682039340299045	34.08074241624875	19.235146318340682
14	20.402398177514456	23.28985322684649	35.69360977867797	20.614138816961084
15	20.65577945822696	23.652692795158366	36.50711832612708	19.1844094204876
16	25.208759088676373	23.467634508367546	33.07307932542161	18.25052707753447
17	25.29001898085541	23.213848949584502	31.57846329862606	19.917668770934025
18	25.444048925732098	23.458133973709796	32.246431740679874	18.851385359878233
19	24.262546264571696	25.36511363245868	30.722808762322902	19.64953134064672
20	24.648025404833955	26.332551055266833	30.996707155115434	18.02271638478378
21	24.607597597779705	24.511581556025867	33.00627237426447	17.874548471929963
22	23.8706997446984	22.559221683858592	33.50222049730245	20.067858074140553
23	22.223165537720156	24.054646266795228	33.479176647281534	20.243011548203086
24	22.1631302442446	24.57838850718301	33.18971354877312	20.068767699799274
25	23.176048949988783	24.894533958347232	31.203293249162638	20.726123842501348
26	21.798976772203456	25.53005908524001	30.484688978773377	22.186275163783154
27	21.10705485447	24.262647334089333	33.19971943101904	21.430578380421622
28	20.391785878162718	24.76809599178507	32.62129858159039	22.218819548461823
29	22.025877839295426	24.895847862076494	32.15840019081925	20.919874107808834
30	23.96499760465243	23.429632369736552	32.44988467968038	20.15548534593064
31	23.892935038578234	23.778726483649983	32.152740297831656	20.175598179940128
32	24.364626477383673	23.61883450675043	31.74765367114809	20.268885344717805
33	24.091233432179322	24.203117388201953	31.423018380502477	20.282630799116248
34	21.76501741427789	24.45953075444352	33.30847023199497	20.466981599283617
35	22.079848961712845	26.004580470539246	31.673670784238816	20.241899783509094
36	23.195959644963	26.276558542496698	30.29356652092442	20.23391529161588
37	21.889029712416793	26.662037682758953	30.346526948165486	21.102405656658764
38	22.814624354923804	26.148200255099464	30.395747803254036	20.6414275867227
39	22.31513879876857	25.541378871215198	29.350891129936997	22.792591200079237
40	24.277201344628864	24.483383160605527	29.928503423224562	21.310912071541047
41	21.65838907317231	24.340470862668763	30.596674004313645	23.404466059845284
42	21.13019977400856	24.900901337958274	31.263126403602925	22.705772484430238
43	21.636558057363015	24.419709364495088	31.35712105500405	22.586611523137844
44	22.213260724991866	24.86107994800984	30.684200206586095	22.241459120412202
45	21.9673585885844	24.819237167708692	29.636715725810525	23.57668851789638
46	21.689720623639353	25.309121119688545	30.05332427750455	22.94783397916755
47	21.21388533461085	25.6313307419109	31.345093782405414	21.80969014107283
48	21.413194423388298	25.279810959574217	31.432619984677864	21.87437463235963
49	22.185163399089163	24.495208294168897	31.206931751797523	22.11269655494442
50	20.872573573555364	25.381587963833287	31.26100394373258	22.484834518878774
51	21.333248434938522	24.8901879690889	30.471347802445475	23.305215793527104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	639.0
1	1346.0
2	2053.0
3	14862.0
4	27671.0
5	18295.5
6	8920.0
7	7800.0
8	6680.0
9	6460.0
10	6240.0
11	5974.0
12	5708.0
13	5450.5
14	5193.0
15	4886.5
16	4580.0
17	4514.0
18	4448.0
19	4373.5
20	4299.0
21	4447.0
22	4595.0
23	5185.0
24	5775.0
25	6801.0
26	9342.0
27	10857.0
28	12333.5
29	13810.0
30	15998.5
31	18187.0
32	21032.5
33	23878.0
34	26999.5
35	30121.0
36	32476.5
37	34832.0
38	37313.5
39	39795.0
40	42282.5
41	44770.0
42	47196.5
43	49623.0
44	54282.0
45	58941.0
46	63466.0
47	67991.0
48	69658.5
49	71326.0
50	70349.0
51	69372.0
52	65513.0
53	61654.0
54	59301.0
55	56948.0
56	55172.0
57	53396.0
58	51252.5
59	49109.0
60	45520.5
61	41932.0
62	36845.5
63	31759.0
64	27518.0
65	23277.0
66	19867.0
67	16457.0
68	13672.0
69	10887.0
70	8596.5
71	6306.0
72	5181.0
73	4056.0
74	3269.0
75	1979.5
76	1477.0
77	1110.5
78	744.0
79	558.5
80	373.0
81	291.5
82	210.0
83	156.0
84	102.0
85	80.5
86	59.0
87	42.5
88	26.0
89	27.0
90	28.0
91	14.5
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	989418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.041282450228046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21112471103521	28.230863560815887
2	6.954715992144016	5.291326308365369
3	3.221176525159607	3.6761305804692226
4	2.1924255636939853	3.336107204783334
5	1.7772681132946122	3.380477914381212
6	1.43111953323652	3.2664973430333393
7	1.2716448371773355	3.3862500299206095
8	1.1291002461471158	3.4361937102643556
9	1.0152384538934573	3.475887550100445
>10	6.759169203488449	39.18510491143987
>50	0.023064485559119458	0.5799503775452832
>100	0.013098110568451525	0.9482274255698524
>500	2.847415340312935E-4	0.10019538137290983
>1k	0.0	0.0
>5k	5.69483068062587E-4	1.706787701938314
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9929	1.0035192406040723	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5828	0.5890331487803941	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013139037292630617	0.0
2	0.0	0.0	0.0	0.0903561487662444	0.0
3	0.0	0.0	0.0	0.17707379489760647	0.0
4	0.0	0.0	0.0	0.23013529165630703	0.0
5	0.0	0.0	0.0	0.4377320808798708	0.0
6	0.0	0.0	0.0	0.7695433072776117	0.0
7	0.0	0.0	0.0	1.0297973151893336	0.0
8	0.0	0.0	0.0	1.526149716297864	0.0
9	0.0	0.0	0.0	1.8713021190235068	0.0
10	0.0	0.0	0.0	2.370888744696377	0.0
11	0.0	0.0	0.0	2.904030450224273	0.0
12	0.0	0.0	0.0	3.2733384676648294	0.0
13	0.0	0.0	0.0	3.468099428148669	0.0
14	0.0	0.0	0.0	3.623746485307524	0.0
15	0.0	0.0	0.0	3.748870548140422	0.0
16	0.0	0.0	0.0	3.941003701165736	0.0
17	0.0	0.0	0.0	4.152340062541818	0.0
18	0.0	0.0	0.0	4.398646477019824	0.0
19	0.0	0.0	0.0	4.554192464661043	0.0
20	0.0	0.0	0.0	4.74278818456911	0.0
21	0.0	0.0	0.0	4.951496738486666	0.0
22	0.0	0.0	0.0	5.172333634520496	0.0
23	0.0	0.0	0.0	5.396000477048124	0.0
24	0.0	0.0	0.0	5.580250207697858	0.0
25	0.0	0.0	0.0	5.76065929667744	0.0
26	0.0	0.0	0.0	5.955622396196552	0.0
27	0.0	0.0	0.0	6.181714907147434	0.0
28	0.0	0.0	0.0	6.3675817500793395	0.0
29	0.0	0.0	0.0	6.57133789763275	0.0
30	0.0	0.0	0.0	6.8240116917218	0.0
31	0.0	0.0	0.0	7.047476395214156	0.0
32	1.0106951763562014E-4	0.0	0.0	7.300958745444292	0.0
33	1.0106951763562014E-4	0.0	0.0	7.528466229642072	0.0
34	1.0106951763562014E-4	0.0	0.0	7.753143767346056	0.0
35	1.0106951763562014E-4	0.0	0.0	8.001774780729681	0.0
36	1.0106951763562014E-4	0.0	0.0	8.236559270197228	0.0
37	1.0106951763562014E-4	0.0	0.0	8.503584935790535	0.0
38	1.0106951763562014E-4	0.0	0.0	8.80507530689759	0.0
39	1.0106951763562014E-4	0.0	0.0	9.289703643960388	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACC	20	7.03281E-4	45.0	36
TGCGTTG	25	3.89041E-5	45.0	1
CGTTTTA	2200	0.0	43.05682	1
CGTTATT	850	0.0	42.61765	1
CGTTTTT	3720	0.0	42.15726	1
CGACTAG	45	1.927765E-8	40.0	2
GCTACGA	115	0.0	39.130436	10
AACACGT	95	0.0	37.894737	41
ATAACGG	30	1.1398073E-4	37.499996	2
GTTTTAT	2645	0.0	37.00378	2
GCCGATT	55	2.748493E-9	36.818184	9
AGGCGAT	525	0.0	36.42857	7
GACCGAT	355	0.0	36.126762	9
ACACGAC	100	0.0	36.0	26
TGCTCGA	25	0.002106967	36.0	34
CTCGATC	50	4.877802E-8	36.0	11
CCGCACG	25	0.002106967	36.0	39
GCGTAAA	50	4.877802E-8	36.0	1
CATAGGA	260	0.0	35.480766	4
TTAACGG	70	2.1827873E-11	35.357143	2
>>END_MODULE