FastQCFastQC Report
Sat 14 Jan 2017
SRR2934593.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934593.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768390
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.28019625450617525No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.16124624214266192No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAA253.8895938E-545.01
CGACGGT1000.042.7528
CGTTTTA4550.041.538461
CGCATAG556.002665E-1140.9090922
CGAATAT1050.040.71428714
CGGTCTA1050.040.71428731
TATACGG501.0804797E-940.52
TCACGGG850.039.7058833
AGGCGAT4650.039.677427
TCACGAC1150.039.13043625
ACGGTCT1100.038.86363630
TTACACG4600.038.64130834
ATGGGCG1400.038.5714265
CGGGATA1400.038.5714266
TAAGGAC2250.038.05
CGTTATT3400.037.720591
GTCTTAC4900.037.6530631
ACACGCG4550.037.58241736
TATTACG301.13956965E-437.5000041
GTCGGCC301.13956965E-437.50000435
Produced by FastQC (version 0.11.5)