##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934593.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	768390
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01038795403376	33.0	31.0	34.0	30.0	34.0
2	32.21118312315361	33.0	31.0	34.0	30.0	34.0
3	32.097883887088585	33.0	31.0	34.0	30.0	34.0
4	35.84280248311404	37.0	35.0	37.0	35.0	37.0
5	32.12898397948958	37.0	35.0	37.0	0.0	37.0
6	33.9882702794154	37.0	35.0	37.0	19.0	37.0
7	20.682124962584105	32.0	0.0	37.0	0.0	37.0
8	28.06385429274197	35.0	17.0	37.0	17.0	37.0
9	34.8289488410833	37.0	32.0	39.0	32.0	39.0
10	36.27247101081482	37.0	35.0	39.0	32.0	39.0
11	36.75773890862713	39.0	37.0	39.0	33.0	39.0
12	37.059830294511904	39.0	37.0	39.0	34.0	39.0
13	36.79840836033785	39.0	37.0	39.0	33.0	39.0
14	38.02845299912805	40.0	37.0	41.0	33.0	41.0
15	38.23949817150145	40.0	38.0	41.0	33.0	41.0
16	38.35374744595843	40.0	38.0	41.0	34.0	41.0
17	38.342453701896176	40.0	38.0	41.0	34.0	41.0
18	38.24087507645857	40.0	37.0	41.0	34.0	41.0
19	38.22679758976562	40.0	37.0	41.0	34.0	41.0
20	38.19760668410573	40.0	37.0	41.0	34.0	41.0
21	38.11130155259699	40.0	37.0	41.0	34.0	41.0
22	38.14138132979346	40.0	37.0	41.0	34.0	41.0
23	38.08293184450604	40.0	37.0	41.0	34.0	41.0
24	37.987330652403074	40.0	37.0	41.0	34.0	41.0
25	37.85671078488788	40.0	36.0	41.0	33.0	41.0
26	37.72383555225862	40.0	36.0	41.0	33.0	41.0
27	37.68257785759835	40.0	36.0	41.0	33.0	41.0
28	37.597650932469186	40.0	36.0	41.0	33.0	41.0
29	37.525802001587735	40.0	36.0	41.0	33.0	41.0
30	37.36117856817501	40.0	36.0	41.0	32.0	41.0
31	37.25553039472143	40.0	36.0	41.0	32.0	41.0
32	37.14247452465545	39.0	35.0	41.0	32.0	41.0
33	36.99989198193626	39.0	35.0	41.0	31.0	41.0
34	36.8325147386093	39.0	35.0	41.0	31.0	41.0
35	36.692009266127876	39.0	35.0	41.0	30.0	41.0
36	36.549605018284986	39.0	35.0	41.0	30.0	41.0
37	36.48039016645193	39.0	35.0	41.0	30.0	41.0
38	36.2945678626739	39.0	35.0	41.0	30.0	41.0
39	36.181605695024665	39.0	35.0	41.0	29.0	41.0
40	36.083704889444164	39.0	35.0	41.0	29.0	41.0
41	35.944723382657244	39.0	35.0	40.0	28.0	41.0
42	35.8441494553547	39.0	35.0	40.0	28.0	41.0
43	35.785399341480236	39.0	35.0	40.0	27.0	41.0
44	35.63647106287172	38.0	35.0	40.0	27.0	41.0
45	35.555482242090605	38.0	35.0	40.0	27.0	41.0
46	35.457239162404505	38.0	34.0	40.0	26.0	41.0
47	35.387881154101436	38.0	34.0	40.0	26.0	41.0
48	35.256565025572954	38.0	34.0	40.0	26.0	41.0
49	35.15237444526868	38.0	34.0	40.0	26.0	41.0
50	34.97992946290295	38.0	34.0	40.0	25.0	41.0
51	33.13957755827119	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	22.0
9	43.0
10	47.0
11	27.0
12	23.0
13	13.0
14	32.0
15	48.0
16	74.0
17	152.0
18	280.0
19	499.0
20	899.0
21	1505.0
22	2423.0
23	3611.0
24	5177.0
25	7133.0
26	9006.0
27	9972.0
28	10977.0
29	12618.0
30	15400.0
31	20089.0
32	26567.0
33	38194.0
34	51580.0
35	65957.0
36	88438.0
37	147486.0
38	151829.0
39	98255.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.54555629302828	21.444318640273817	25.525709600593448	12.484415466104453
2	32.8533687320241	24.41976079855282	26.029490232824475	16.6973802365986
3	32.73337758169679	23.298585353791694	27.4971043350382	16.470932729473315
4	28.72655812803394	25.286247868920732	26.865914444487828	19.121279558557504
5	23.05326722107263	36.88387407436328	22.934447350954592	17.128411353609497
6	27.718866721326407	33.860019000767835	24.901807675789637	13.519306602116114
7	49.724619008576376	43.09426202839704	4.751493382266818	2.42962558075977
8	85.96181626517783	4.394643345176278	6.83702286599253	2.806517523653353
9	78.49555564231704	8.11658142349588	8.92554562136415	4.462317312822915
10	37.979021070029546	34.84454508778094	15.10209659157459	12.074337250614922
11	32.6487851221385	25.318132719061936	25.037806322310285	16.99527583648928
12	29.81402673121722	23.12757844323846	27.99385728601361	19.064537539530708
13	26.880490375980948	23.99471622483374	29.46810864274652	19.65668475643879
14	21.49819752990018	26.359010398365413	31.28216140241284	20.86063066932157
15	20.632491313005115	25.92849985033642	32.901911789586016	20.53709704707245
16	25.99331068858262	25.066567758560105	29.638985411054282	19.301136141802992
17	25.904293392678195	25.282473743801976	27.89221619229818	20.921016671221647
18	26.063977927875165	23.98859953929645	29.966943869649526	19.980478663178854
19	26.26491755488749	24.980673876547066	27.63063027889483	21.12377828967061
20	25.726649227605776	27.06724449823657	27.887531071461108	19.318575202696547
21	25.85692161532555	25.250979320397192	29.941045562800138	18.951053501477112
22	25.237704811358814	23.095693593097256	30.002212418173063	21.664389177370865
23	22.704355860956024	25.82698889886646	30.047632061843593	21.42102317833392
24	22.328895482762658	25.462981038274833	30.39745441767852	21.81066906128398
25	23.30144848319213	26.71586043545595	28.32624058095498	21.656450500396936
26	22.237665768685172	26.9779669178412	27.398846939705095	23.38552037376853
27	21.34358854227671	26.60602038027564	30.039042673642292	22.011348403805357
28	20.66932156847434	26.077773005895445	30.16059553091529	23.092309894714923
29	22.166608102656205	26.444904280378456	29.80322492484285	21.58526269212249
30	23.250823149702626	25.18799047358763	29.82105441247283	21.740131964236912
31	24.189148739572353	25.436952589179974	28.727469123752265	21.646429547495412
32	24.543135647262456	25.810200549200275	28.155493954892698	21.491169848644567
33	24.21804031806765	25.695545230937416	28.22655162092167	21.859862830073272
34	21.727378024180428	25.91639662150731	30.774086076081158	21.582139278231107
35	22.117154049375966	27.162638764169238	29.08965499290725	21.630552193547548
36	23.931987662515127	27.01011205247335	27.521961503923787	21.53593878108773
37	22.279701713973374	28.297088717968737	27.663035698017936	21.760173870039953
38	22.978565572170382	27.891565481070813	27.740730618566094	21.389138328192715
39	22.10752352321087	26.8052681580968	27.960540871172192	23.126667447520138
40	24.513202930803367	25.205039107744764	28.82117154049376	21.46058642095811
41	21.618188680227487	24.555108733846094	29.188563099467718	24.638139486458698
42	22.108304376683716	25.573601946927994	29.17359674123817	23.14449693515012
43	22.056637905230417	25.13892684704382	29.222660367781984	23.58177487994378
44	22.3266830645896	25.951795312276317	29.00050755475735	22.721014068376736
45	21.62274365881909	26.381915433568892	28.247634664688505	23.747706242923517
46	21.7508036283658	26.768307760382097	28.591860903968037	22.88902770728406
47	20.892515519462773	27.198037454938245	29.7852652949674	22.12418173063158
48	22.22543239761059	26.85016723278543	29.274977550462655	21.64942281914132
49	22.76409115162873	26.055388539673864	29.239188432957224	21.941331875740183
50	20.98075196189435	27.407826754642823	28.791629250771095	22.819792032691733
51	21.777222504197088	26.254896601985973	28.109944168976693	23.85793672484025
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	411.0
1	1105.5
2	1800.0
3	4211.0
4	6622.0
5	4603.5
6	2585.0
7	2335.5
8	2086.0
9	2063.5
10	2041.0
11	2035.5
12	2030.0
13	2070.0
14	2110.0
15	2103.5
16	2097.0
17	2118.5
18	2140.0
19	2244.5
20	2349.0
21	2676.5
22	3004.0
23	3549.5
24	4095.0
25	5178.5
26	7628.0
27	8994.0
28	10446.5
29	11899.0
30	13970.0
31	16041.0
32	19021.5
33	22002.0
34	24572.5
35	27143.0
36	28798.0
37	30453.0
38	32367.0
39	34281.0
40	37312.0
41	40343.0
42	42641.5
43	44940.0
44	47108.5
45	49277.0
46	51071.5
47	52866.0
48	54503.0
49	56140.0
50	55453.5
51	54767.0
52	51881.5
53	48996.0
54	46540.0
55	44084.0
56	42819.0
57	41554.0
58	39431.5
59	37309.0
60	34663.5
61	32018.0
62	28513.5
63	25009.0
64	21430.5
65	17852.0
66	15025.0
67	12198.0
68	10254.0
69	8310.0
70	6772.0
71	5234.0
72	4225.0
73	3216.0
74	2493.5
75	1367.5
76	964.0
77	730.5
78	497.0
79	385.5
80	274.0
81	213.5
82	153.0
83	116.0
84	79.0
85	55.5
86	32.0
87	26.5
88	21.0
89	18.5
90	16.0
91	15.0
92	14.0
93	9.0
94	4.0
95	4.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	768390.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.951414599596816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0741952528061	25.149237141595066
2	6.453653693397871	4.382213444535408
3	2.8090830497155608	2.861170297967785
4	2.0067597323128648	2.7252932669412
5	1.4492613768585854	2.460223693445418
6	1.285968518512908	2.6196270200436627
7	1.1438960186735592	2.7185821592169908
8	1.0422429794356007	2.8308498806669715
9	0.9542940466246815	2.9159669544193383
>10	8.728944558945251	49.161270258149266
>50	0.036190540926050827	0.8059818482445632
>100	0.014217712475033306	0.7960350653895056
>500	4.3083977197070624E-4	0.07738010834087623
>1k	8.616795439414125E-4	0.496168861043955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2153	0.28019625450617525	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1239	0.16124624214266192	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022644750712528795	0.0
2	0.0	0.0	0.0	0.11205247335337525	0.0
3	0.0	0.0	0.0	0.1486224443316545	0.0
4	0.0	0.0	0.0	0.19182966982912322	0.0
5	0.0	0.0	0.0	0.3275680318588217	0.0
6	0.0	0.0	0.0	0.4418329233852601	0.0
7	0.0	0.0	0.0	0.5542758234750582	0.0
8	0.0	0.0	0.0	0.8070120641861555	0.0
9	0.0	0.0	0.0	0.9247907963404001	0.0
10	0.0	0.0	0.0	1.2163094262028398	0.0
11	0.0	0.0	0.0	1.6075170160985959	0.0
12	0.0	0.0	0.0	1.9292286469110738	0.0
13	0.0	0.0	0.0	2.0608024570855945	0.0
14	0.0	0.0	0.0	2.1377165241609077	0.0
15	1.301422454743034E-4	0.0	0.0	2.24937857077786	0.0
16	1.301422454743034E-4	0.0	0.0	2.466716120719947	0.0
17	1.301422454743034E-4	0.0	0.0	2.719582503676518	0.0
18	1.301422454743034E-4	0.0	0.0	2.996525202045836	0.0
19	1.301422454743034E-4	0.0	0.0	3.170655526490454	0.0
20	1.301422454743034E-4	0.0	0.0	3.3774515545491224	0.0
21	1.301422454743034E-4	0.0	0.0	3.6238108252319785	0.0
22	1.301422454743034E-4	0.0	0.0	3.8941162690821067	0.0
23	1.301422454743034E-4	0.0	0.0	4.133838285245774	0.0
24	1.301422454743034E-4	0.0	0.0	4.324106248129206	0.0
25	1.301422454743034E-4	0.0	0.0	4.5085178099662935	0.0
26	1.301422454743034E-4	0.0	0.0	4.677312302346465	0.0
27	1.301422454743034E-4	0.0	0.0	4.89217714962454	0.0
28	1.301422454743034E-4	0.0	0.0	5.084917815171983	0.0
29	1.301422454743034E-4	0.0	0.0	5.307330912687568	0.0
30	1.301422454743034E-4	0.0	0.0	5.610041775660798	0.0
31	1.301422454743034E-4	0.0	0.0	5.831804161949011	0.0
32	1.301422454743034E-4	0.0	0.0	6.049011569645622	0.0
33	1.301422454743034E-4	0.0	0.0	6.2673902575515035	0.0
34	1.301422454743034E-4	0.0	0.0	6.504119002069261	0.0
35	1.301422454743034E-4	0.0	0.0	6.779369851247413	0.0
36	1.301422454743034E-4	0.0	0.0	7.000871953044678	0.0
37	2.602844909486068E-4	0.0	0.0	7.245018805554471	0.0
38	2.602844909486068E-4	0.0	0.0	7.524564348833275	0.0
39	2.602844909486068E-4	0.0	0.0	7.990083160894858	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGAA	25	3.8895938E-5	45.0	1
CGACGGT	100	0.0	42.75	28
CGTTTTA	455	0.0	41.53846	1
CGCATAG	55	6.002665E-11	40.909092	2
CGAATAT	105	0.0	40.714287	14
CGGTCTA	105	0.0	40.714287	31
TATACGG	50	1.0804797E-9	40.5	2
TCACGGG	85	0.0	39.705883	3
AGGCGAT	465	0.0	39.67742	7
TCACGAC	115	0.0	39.130436	25
ACGGTCT	110	0.0	38.863636	30
TTACACG	460	0.0	38.641308	34
ATGGGCG	140	0.0	38.571426	5
CGGGATA	140	0.0	38.571426	6
TAAGGAC	225	0.0	38.0	5
CGTTATT	340	0.0	37.72059	1
GTCTTAC	490	0.0	37.65306	31
ACACGCG	455	0.0	37.582417	36
TATTACG	30	1.13956965E-4	37.500004	1
GTCGGCC	30	1.13956965E-4	37.500004	35
>>END_MODULE