FastQCFastQC Report
Sat 14 Jan 2017
SRR2934592.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934592.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639115
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA25160.393669370926985No Hit
GCTCAAATTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA16310.2551966391025089No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT16080.25159791273870896No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14260.2231210345555964No Hit
GCTCAAATTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT9920.15521463273432792No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8820.1380033327335456No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCTCA7200.11265578182330255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGG351.2107921E-745.0000042
GCGCATA207.0309336E-445.01
ATCCGCG207.0309336E-445.01
AAGTGCG207.0309336E-445.01
ACGTAAG302.1639826E-644.9999962
TTGACCC12250.042.9795958
AATTGAC5350.042.0560726
CCTGCAT11950.041.9874513
TGCATTA11750.041.55319215
CATTAGA11550.041.49350717
GAAATTG10150.041.453222
CTCAAAT5550.041.3513532
CTTGACC8000.041.3437547
CGTATAG603.6379788E-1241.2499962
AGGCGAT4000.041.0625047
CTGCATT11900.041.0294114
ATTAGAA11800.040.61440718
ACCCTGC12300.040.60975611
GCTCAAA6050.040.5371931
CGTTTTA3500.040.51
Produced by FastQC (version 0.11.5)