##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934592.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 639115 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92203124633282 31.0 31.0 34.0 30.0 34.0 2 32.12428592663292 33.0 31.0 34.0 30.0 34.0 3 32.05367735071153 33.0 31.0 34.0 30.0 34.0 4 35.80923777410951 37.0 35.0 37.0 35.0 37.0 5 32.12933196686043 37.0 35.0 37.0 10.0 37.0 6 33.979072623862685 37.0 35.0 37.0 19.0 37.0 7 20.708202749114008 32.0 0.0 37.0 0.0 37.0 8 28.052741681856944 35.0 17.0 37.0 17.0 37.0 9 34.79158993295416 37.0 32.0 39.0 32.0 39.0 10 36.225641707673894 37.0 35.0 39.0 32.0 39.0 11 36.612920992309675 38.0 35.0 39.0 32.0 39.0 12 36.957660201998074 39.0 37.0 39.0 33.0 39.0 13 36.69229324925874 39.0 35.0 39.0 33.0 39.0 14 37.99491484318159 40.0 37.0 41.0 33.0 41.0 15 38.19478497609976 40.0 37.0 41.0 33.0 41.0 16 38.27983539738545 40.0 37.0 41.0 33.0 41.0 17 38.27238446914875 40.0 37.0 41.0 34.0 41.0 18 38.160891232407316 40.0 37.0 41.0 33.0 41.0 19 38.14092925373368 40.0 37.0 41.0 34.0 41.0 20 38.10745640455943 40.0 37.0 41.0 34.0 41.0 21 38.01012024440046 40.0 37.0 41.0 33.0 41.0 22 38.00214202451828 40.0 37.0 41.0 33.0 41.0 23 37.94087292584277 40.0 36.0 41.0 33.0 41.0 24 37.816491554728024 40.0 36.0 41.0 33.0 41.0 25 37.66852444395766 40.0 36.0 41.0 33.0 41.0 26 37.53791884089718 39.0 36.0 41.0 33.0 41.0 27 37.52677061248758 39.0 36.0 41.0 33.0 41.0 28 37.40864007260039 39.0 36.0 41.0 33.0 41.0 29 37.366484904907566 39.0 36.0 41.0 32.0 41.0 30 37.210143714355006 39.0 35.0 41.0 32.0 41.0 31 37.07881054270358 39.0 35.0 41.0 31.0 41.0 32 36.98925232548133 39.0 35.0 41.0 31.0 41.0 33 36.85210642842055 39.0 35.0 41.0 31.0 41.0 34 36.664487611775655 39.0 35.0 41.0 30.0 41.0 35 36.519945549705454 39.0 35.0 41.0 30.0 41.0 36 36.355641785907075 39.0 35.0 40.0 30.0 41.0 37 36.26727897170306 39.0 35.0 40.0 30.0 41.0 38 36.111310171095965 39.0 35.0 40.0 29.0 41.0 39 35.993160855245144 39.0 35.0 40.0 29.0 41.0 40 35.85170118053871 39.0 35.0 40.0 28.0 41.0 41 35.715406460496155 38.0 35.0 40.0 27.0 41.0 42 35.60639634494574 38.0 35.0 40.0 27.0 41.0 43 35.53682201168804 38.0 34.0 40.0 27.0 41.0 44 35.36699342058941 38.0 34.0 40.0 26.0 41.0 45 35.255328070847966 38.0 34.0 40.0 26.0 41.0 46 35.13584722624254 38.0 34.0 40.0 26.0 41.0 47 35.04208006383828 38.0 34.0 40.0 25.0 41.0 48 34.92257418461466 38.0 34.0 40.0 25.0 41.0 49 34.791025089381414 37.0 34.0 40.0 24.0 41.0 50 34.592536554454206 37.0 34.0 40.0 24.0 41.0 51 32.606620091845755 35.0 31.0 39.0 18.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 4.0 8 22.0 9 48.0 10 45.0 11 26.0 12 10.0 13 15.0 14 23.0 15 35.0 16 61.0 17 133.0 18 258.0 19 447.0 20 713.0 21 1227.0 22 1954.0 23 3007.0 24 4285.0 25 5704.0 26 7272.0 27 8477.0 28 9617.0 29 11688.0 30 14331.0 31 18435.0 32 24133.0 33 34652.0 34 46732.0 35 59253.0 36 76264.0 37 120248.0 38 116932.0 39 73052.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.92688326826941 20.49020911729501 24.5710083474805 13.011899266955087 2 30.318643749559936 23.613121269255142 26.105473975732068 19.962761005452855 3 30.82183957503736 23.76708417108032 29.109471691323158 16.301604562559163 4 27.40617885670028 25.801772763900082 26.351595565743253 20.440452813656385 5 21.99025214554501 38.01334658081879 22.471229747385056 17.525171526251142 6 26.057595268457163 35.80153806435462 24.009450568364066 14.131416098824158 7 48.656970967666226 43.01495036104613 4.487611775658528 3.840466895629112 8 84.1873528238267 3.934816112906128 8.793409636763338 3.0844214265038374 9 78.61245628720965 6.535130610297052 10.402353254109197 4.450059848384093 10 44.78051680839911 27.119845411232717 14.952864507952404 13.146773272415762 11 31.90161395054098 26.62916689484678 22.885083279222048 18.58413587539019 12 28.68357024948562 21.757117263716232 28.129992254915 21.42932023188315 13 25.250385298420476 23.025903006501178 29.64615131862028 22.077560376458074 14 21.194151287327006 24.055764612002534 30.540982452297317 24.209101648373142 15 20.51806013002355 23.523778975614718 35.03266235341058 20.92549854095116 16 26.514007651205183 22.821245002855512 30.088012329549457 20.57673501638985 17 25.412797383882403 23.235880866510723 26.671569279394163 24.679752470212716 18 25.04181563568372 25.891115057540503 28.600643076754572 20.466426230021202 19 25.124273409323834 25.40872925842767 27.885591794903892 21.581405537344608 20 26.097337724822605 25.628877432074038 28.979135210408142 19.29464963269521 21 26.125658136642077 26.382419439381017 27.832862630356036 19.659059793620866 22 26.684868920303856 23.16390633923472 28.070378570366834 22.080846170094585 23 24.201278330190966 25.51277939025058 28.531172011296874 21.75477026826158 24 24.469461677475884 24.835436502037975 28.859281975857243 21.8358198446289 25 23.958129601089006 26.101875249368266 27.09105560032232 22.848939549220407 26 22.703112898304685 25.091259006595056 28.602051274027367 23.60357682107289 27 21.99917072827269 24.961078992043685 29.58951049498134 23.450239784702283 28 21.988374549181287 24.884723406585668 28.802328219491013 24.32457382474203 29 21.335127480969778 25.510588861159572 28.86162897131189 24.292654686558755 30 22.70107883557732 25.576617666617118 28.386597091290298 23.335706406515257 31 24.692269779304194 25.33112194206051 27.03206778122873 22.94454049740657 32 24.543470267479247 25.243813711147446 26.762476236671024 23.450239784702283 33 23.821065066537322 24.949500481134066 26.17713557028078 25.05229888204783 34 21.732864977351493 26.12722280027851 29.19208593132691 22.947826291043082 35 23.02559007377389 26.395875546654356 28.010295486727742 22.56823889284401 36 23.35010131197046 27.31214257215055 26.57925412484451 22.75850199103448 37 22.451827918293265 29.084906472231133 26.051806013002356 22.41145959647325 38 21.9545778146343 28.47171479311235 25.51528285206888 24.058424540184472 39 22.09367641191335 26.56016522848001 26.417937303928092 24.928221055678556 40 23.391408431972337 24.388412101108564 28.590785695845035 23.629393771074064 41 20.952723688225124 26.267416662103066 27.233909390328815 25.545950259342998 42 21.23123381551051 25.003324910227427 27.18556128396298 26.579879990299087 43 21.557935582798088 25.036339312956198 27.907497085813976 25.49822801843174 44 21.73552490553343 24.69555557294071 28.48391916947654 25.08500035204932 45 21.48330112734015 26.57925412484451 26.575185999389785 25.36225874842556 46 21.74804221462491 25.82868497844676 27.773248945807875 24.650023861120456 47 21.079304976412697 24.881437612949156 29.870524084085027 24.168733326553124 48 21.004670520954758 25.069197249321327 29.236209445874373 24.689922783849543 49 22.01716436009169 25.42140303388279 28.465456138566616 24.095976467458907 50 20.698935246395404 25.279800974785445 29.09085219404958 24.930411584769562 51 21.03862372186539 25.937585567542616 27.429335878519517 25.59445483207248 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 405.0 1 1041.0 2 1677.0 3 3149.0 4 4621.0 5 3231.0 6 1841.0 7 1686.0 8 1531.0 9 1514.5 10 1498.0 11 1505.0 12 1512.0 13 1496.5 14 1481.0 15 1401.5 16 1322.0 17 1339.0 18 1356.0 19 1455.5 20 1555.0 21 1985.0 22 2415.0 23 2652.0 24 2889.0 25 3430.0 26 4771.0 27 5571.0 28 7530.5 29 9490.0 30 11368.5 31 13247.0 32 14195.5 33 15144.0 34 16771.5 35 18399.0 36 19978.5 37 21558.0 38 23928.0 39 26298.0 40 27943.0 41 29588.0 42 32055.0 43 34522.0 44 37304.5 45 40087.0 46 42639.5 47 45192.0 48 46127.5 49 47063.0 50 45926.0 51 44789.0 52 42653.0 53 40517.0 54 38921.5 55 37326.0 56 36522.0 57 35718.0 58 35482.0 59 35246.0 60 33117.5 61 30989.0 62 28140.0 63 25291.0 64 22695.0 65 20099.0 66 16902.5 67 13706.0 68 11113.0 69 8520.0 70 7063.0 71 5606.0 72 4474.5 73 3343.0 74 2588.5 75 1352.5 76 871.0 77 664.5 78 458.0 79 368.0 80 278.0 81 195.5 82 113.0 83 98.0 84 83.0 85 59.0 86 35.0 87 34.5 88 34.0 89 22.0 90 10.0 91 9.5 92 9.0 93 6.5 94 4.0 95 2.5 96 1.0 97 0.5 98 0.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 639115.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.922753849384385 #Duplication Level Percentage of deduplicated Percentage of total 1 74.2879360879196 27.429151781530543 2 7.115500420803243 5.254477411050183 3 3.24034157458556 3.5892700303904763 4 2.0809928923798693 3.0734395331064137 5 1.681973985902208 3.105155573126757 6 1.328174710975683 2.942392075339943 7 1.1054590689684884 2.8571615165865185 8 1.0292589429966847 3.0402459679635307 9 0.9512748028851362 3.1611316851044577 >10 7.121220249957008 41.76231317854015 >50 0.039200403715595876 0.9478660834860153 >100 0.015400158601524641 1.1530848628154728 >500 0.0014000144183204217 0.4469668461062965 >1k 0.0018666858910938958 1.2373434548532443 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA 2516 0.393669370926985 No Hit GCTCAAATTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA 1631 0.2551966391025089 No Hit GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT 1608 0.25159791273870896 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1426 0.2231210345555964 No Hit GCTCAAATTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT 992 0.15521463273432792 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 882 0.1380033327335456 No Hit GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCTCA 720 0.11265578182330255 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.129327272869515E-4 0.0 0.0 0.04224591818373845 0.0 2 3.129327272869515E-4 0.0 0.0 0.19464415637248383 0.0 3 3.129327272869515E-4 0.0 0.0 0.25644837001165677 0.0 4 3.129327272869515E-4 0.0 0.0 0.3240418391056383 0.0 5 3.129327272869515E-4 0.0 0.0 0.5818984063900863 0.0 6 3.129327272869515E-4 0.0 0.0 0.7737261682169876 0.0 7 3.129327272869515E-4 0.0 0.0 0.9808876336809494 0.0 8 3.129327272869515E-4 0.0 0.0 1.5078663464321758 0.0 9 3.129327272869515E-4 0.0 0.0 1.7034493009865204 0.0 10 3.129327272869515E-4 0.0 0.0 2.172378992826017 0.0 11 3.129327272869515E-4 0.0 0.0 2.7802508155809207 0.0 12 3.129327272869515E-4 0.0 0.0 3.267174139239417 0.0 13 3.129327272869515E-4 0.0 0.0 3.605923816527542 0.0 14 3.129327272869515E-4 0.0 0.0 3.6918238501678102 0.0 15 3.129327272869515E-4 0.0 0.0 3.9438911619974495 0.0 16 3.129327272869515E-4 0.0 0.0 4.224435352010202 0.0 17 3.129327272869515E-4 0.0 0.0 4.557552240207161 0.0 18 3.129327272869515E-4 0.0 0.0 4.937609037497164 0.0 19 3.129327272869515E-4 0.0 0.0 5.189363416599517 0.0 20 3.129327272869515E-4 0.0 0.0 5.496506888431659 0.0 21 3.129327272869515E-4 0.0 0.0 5.822426323901019 0.0 22 3.129327272869515E-4 0.0 0.0 6.199510260281796 0.0 23 3.129327272869515E-4 0.0 0.0 6.5715872730259814 0.0 24 3.129327272869515E-4 0.0 0.0 6.872315623948742 0.0 25 3.129327272869515E-4 0.0 0.0 7.342497046697386 0.0 26 3.129327272869515E-4 0.0 0.0 7.604578205800208 0.0 27 3.129327272869515E-4 0.0 0.0 7.907340619450333 0.0 28 3.129327272869515E-4 0.0 0.0 8.147672954006712 0.0 29 3.129327272869515E-4 0.0 0.0 8.434006399474272 0.0 30 3.129327272869515E-4 0.0 0.0 8.78589925130845 0.0 31 3.129327272869515E-4 0.0 0.0 9.050796804956855 0.0 32 3.129327272869515E-4 0.0 0.0 9.377185639517144 0.0 33 3.129327272869515E-4 0.0 0.0 9.652097040438731 0.0 34 3.129327272869515E-4 0.0 0.0 10.061882446820995 0.0 35 3.129327272869515E-4 0.0 0.0 10.372624645016938 0.0 36 3.129327272869515E-4 0.0 0.0 10.650978305938681 0.0 37 3.129327272869515E-4 0.0 0.0 10.93856348231539 0.0 38 3.129327272869515E-4 0.0 0.0 11.26229238869374 0.0 39 3.129327272869515E-4 0.0 0.0 11.727623354169438 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCGG 35 1.2107921E-7 45.000004 2 GCGCATA 20 7.0309336E-4 45.0 1 ATCCGCG 20 7.0309336E-4 45.0 1 AAGTGCG 20 7.0309336E-4 45.0 1 ACGTAAG 30 2.1639826E-6 44.999996 2 TTGACCC 1225 0.0 42.979595 8 AATTGAC 535 0.0 42.056072 6 CCTGCAT 1195 0.0 41.98745 13 TGCATTA 1175 0.0 41.553192 15 CATTAGA 1155 0.0 41.493507 17 GAAATTG 1015 0.0 41.4532 22 CTCAAAT 555 0.0 41.351353 2 CTTGACC 800 0.0 41.343754 7 CGTATAG 60 3.6379788E-12 41.249996 2 AGGCGAT 400 0.0 41.062504 7 CTGCATT 1190 0.0 41.02941 14 ATTAGAA 1180 0.0 40.614407 18 ACCCTGC 1230 0.0 40.609756 11 GCTCAAA 605 0.0 40.537193 1 CGTTTTA 350 0.0 40.5 1 >>END_MODULE