##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934591.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	188905
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.770090786374105	31.0	31.0	34.0	30.0	34.0
2	31.951615891585718	33.0	31.0	34.0	30.0	34.0
3	31.723633572430586	31.0	31.0	34.0	30.0	34.0
4	35.58877742780763	37.0	35.0	37.0	33.0	37.0
5	32.0417140890924	37.0	35.0	37.0	10.0	37.0
6	33.886704957518326	37.0	35.0	37.0	19.0	37.0
7	20.81786612318361	32.0	0.0	37.0	0.0	37.0
8	28.084386331754057	35.0	17.0	37.0	17.0	37.0
9	34.72357004843705	37.0	32.0	39.0	30.0	39.0
10	36.156708398401314	37.0	35.0	39.0	32.0	39.0
11	36.563055504089355	38.0	35.0	39.0	32.0	39.0
12	36.8121013207697	39.0	35.0	39.0	33.0	39.0
13	36.44438739048728	39.0	35.0	39.0	32.0	39.0
14	37.73725417537916	40.0	36.0	41.0	33.0	41.0
15	37.95274873613721	40.0	37.0	41.0	33.0	41.0
16	38.0915698366904	40.0	37.0	41.0	33.0	41.0
17	38.03236547470951	40.0	36.0	41.0	33.0	41.0
18	37.934056801037556	40.0	36.0	41.0	33.0	41.0
19	37.86902940631534	40.0	36.0	41.0	33.0	41.0
20	37.84702363621926	39.0	36.0	41.0	34.0	41.0
21	37.757666551970566	39.0	36.0	41.0	33.0	41.0
22	37.73264339218125	39.0	36.0	41.0	33.0	41.0
23	37.71996506180355	39.0	35.0	41.0	33.0	41.0
24	37.55134062094704	39.0	35.0	41.0	33.0	41.0
25	37.42509197744898	39.0	35.0	41.0	33.0	41.0
26	37.26368809719171	39.0	35.0	41.0	33.0	41.0
27	37.227452952542286	39.0	35.0	41.0	33.0	41.0
28	37.181636272200315	39.0	35.0	41.0	33.0	41.0
29	37.21669622296922	39.0	35.0	41.0	33.0	41.0
30	37.06490564040126	39.0	35.0	41.0	32.0	41.0
31	36.924893464969166	39.0	35.0	40.0	32.0	41.0
32	36.78917445276726	39.0	35.0	40.0	31.0	41.0
33	36.6056324607607	39.0	35.0	40.0	31.0	41.0
34	36.381445700219686	39.0	35.0	40.0	30.0	41.0
35	36.23987718694582	39.0	35.0	40.0	30.0	41.0
36	36.030713850877426	39.0	35.0	40.0	29.0	41.0
37	35.88016727984966	39.0	35.0	40.0	29.0	41.0
38	35.80604007305259	38.0	35.0	40.0	29.0	41.0
39	35.7189698525714	38.0	35.0	40.0	28.0	41.0
40	35.612260130753555	38.0	35.0	40.0	27.0	41.0
41	35.51316799449459	38.0	35.0	40.0	27.0	41.0
42	35.437722664831526	38.0	35.0	40.0	26.0	41.0
43	35.343934782033294	38.0	35.0	40.0	26.0	41.0
44	35.14455414097033	38.0	34.0	40.0	25.0	41.0
45	35.03889785871205	38.0	34.0	40.0	25.0	41.0
46	34.85935258463249	38.0	34.0	40.0	24.0	41.0
47	34.81852783144967	38.0	34.0	40.0	24.0	41.0
48	34.61963420767052	38.0	34.0	40.0	23.0	41.0
49	34.510288240120694	37.0	34.0	40.0	23.0	41.0
50	34.25560996267966	37.0	33.0	40.0	22.0	41.0
51	32.10862602895635	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	10.0
10	7.0
11	10.0
12	3.0
13	2.0
14	2.0
15	8.0
16	16.0
17	26.0
18	69.0
19	124.0
20	223.0
21	365.0
22	653.0
23	905.0
24	1545.0
25	2144.0
26	2579.0
27	2817.0
28	3125.0
29	3569.0
30	4317.0
31	5611.0
32	7696.0
33	11190.0
34	15226.0
35	19113.0
36	21332.0
37	33899.0
38	32552.0
39	19761.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.520340912098675	19.436753923930016	24.157645377306054	14.885259786665255
2	32.702151875281224	25.070802784468384	26.240173632249014	15.986871708001377
3	30.068552976363783	24.94163733093354	29.872157962997274	15.11765172970541
4	25.827267674227787	25.553055768772666	30.614859320822635	18.004817236176915
5	23.125909848865835	34.70368703845848	25.66581085730923	16.504592255366454
6	24.57531563484291	34.22619835366984	27.614409359201712	13.58407665228554
7	46.144358275323576	42.72782615600434	8.3984013128292	2.729414255842884
8	79.20489134750271	5.564701834255314	11.162224398507187	4.068182419734788
9	73.9673380799873	7.016754453296631	12.234191789523836	6.781715677192239
10	41.35570789550303	26.3598104867526	18.948677906884413	13.335803710859956
11	33.04094650750377	21.99147719753315	27.386252349064343	17.581323945898735
12	30.512162197930177	21.200603477938646	31.170694264312747	17.116540059818426
13	23.23601810433816	27.22903046504857	32.5444006246526	16.99055080596067
14	17.978878272147377	29.461898838040284	31.826579497631087	20.732643392181256
15	17.017019136603054	23.847965908790133	41.096847621820494	18.03816733278632
16	20.491252216722692	22.21328180831635	35.86088245414361	21.43458352081734
17	19.875598845980786	22.581720970858367	32.18072576162621	25.361954421534634
18	21.412350123077736	23.913607368783254	33.58725285196263	21.086789656176386
19	22.810936714221434	25.457769778460072	30.358116513591487	21.373176993727007
20	24.681188957412456	24.80188454514174	31.58783515523676	18.92909134220905
21	22.218575474444826	26.153886874354836	31.75564437150949	19.87189327969085
22	21.173076414070565	24.020010057965642	30.49945739922183	24.30745612874196
23	19.747492125671634	26.225351367089278	31.258039755432627	22.769116751806465
24	21.114316720044467	23.5525793388211	32.884253990100845	22.44884995103359
25	18.546888647732988	26.63508112543342	32.595749186098836	22.222281040734764
26	19.91424260871867	25.630872660861282	32.16802096291787	22.286863767502183
27	19.8369550832429	27.037399751197693	31.792700034408828	21.33294513115058
28	16.600407612291892	26.715015483973424	34.57134538524655	22.11323151848813
29	19.47116275376512	24.083004684894522	35.0154839734258	21.430348587914562
30	21.815727482067707	25.624520261507104	32.21672269129986	20.343029565125327
31	22.901987771631244	25.331251157989463	30.23530345941082	21.531457610968477
32	24.25504883407004	25.22802466848416	31.54072152669331	18.976204970752494
33	24.140705645694926	23.50864190995474	28.83883433471851	23.511818109631825
34	19.47592705328075	24.724067653053122	33.02982980863397	22.77017548503216
35	20.769699055080597	23.47105688044255	32.4522908340171	23.306953230459754
36	23.836319843307482	24.21217013842937	30.244302691829226	21.707207326433924
37	20.65482650009264	26.44292104496969	33.37233000714645	19.52992244779122
38	20.921627272967893	26.756835446388394	29.11092877372224	23.210608506921467
39	21.156136682459437	25.41224424975517	32.33159524628781	21.10002382149758
40	22.77017548503216	23.18043460998915	32.74344247108335	21.305947433895344
41	19.808898652761968	23.234430004499615	30.61591805404833	26.340753288690085
42	20.61035970461343	23.59651676768746	31.78846510150605	24.00465842619306
43	22.629893332627514	23.066620788226885	29.45342897223472	24.85005690691088
44	20.695587729281915	25.065509118339907	29.954209787988674	24.284693364389508
45	20.264153939811017	25.579524099415053	29.566713427384133	24.589608533389796
46	22.801937481803023	26.728779015907467	29.354437415632194	21.114846086657316
47	19.53151054762976	24.197347873269635	33.8154098621	22.45573171700061
48	20.58918504009952	23.394828088192476	31.76411423731505	24.25187263439295
49	20.827400015880997	22.436145152325242	33.20505015748657	23.53140467430719
50	19.83960191630714	24.78335671369207	31.821285831502603	23.55575553849819
51	20.785580053466028	23.270426934173262	29.81710383526111	26.1268891770996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	326.0
1	420.0
2	514.0
3	1797.5
4	3081.0
5	2146.0
6	1211.0
7	1108.0
8	1005.0
9	974.5
10	944.0
11	929.5
12	915.0
13	912.5
14	910.0
15	873.5
16	837.0
17	774.0
18	711.0
19	861.0
20	1011.0
21	984.0
22	957.0
23	944.5
24	932.0
25	1084.5
26	1631.0
27	2025.0
28	2171.0
29	2317.0
30	2657.5
31	2998.0
32	3533.0
33	4068.0
34	4707.5
35	5347.0
36	5377.5
37	5408.0
38	6474.5
39	7541.0
40	8689.0
41	9837.0
42	11262.0
43	12687.0
44	14346.0
45	16005.0
46	18294.0
47	20583.0
48	18664.0
49	16745.0
50	15835.0
51	14925.0
52	13102.5
53	11280.0
54	10341.5
55	9403.0
56	8781.5
57	8160.0
58	7455.5
59	6751.0
60	6241.5
61	5732.0
62	4968.0
63	4204.0
64	3598.0
65	2992.0
66	2525.0
67	2058.0
68	1710.5
69	1363.0
70	1033.5
71	704.0
72	574.5
73	445.0
74	373.5
75	258.0
76	214.0
77	152.0
78	90.0
79	74.0
80	58.0
81	55.0
82	52.0
83	31.0
84	10.0
85	8.5
86	7.0
87	3.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	188905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.85971784759535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65233674648393	29.35761361530928
2	8.507643066788848	6.782245043805086
3	3.684077718899823	4.4053889521187894
4	2.269678738860778	3.618750165427066
5	1.673373441172955	3.3350096609406847
6	1.387837496845824	3.3191286625552525
7	1.1142542199556424	3.108970117254705
8	0.9761345073508905	3.112675683544639
9	0.9057465768888535	3.249252269659353
>10	5.767826075408051	34.816971493607895
>50	0.025233409033560434	0.7252322596013869
>100	0.02921763151254366	2.216987374606284
>500	0.005312296638644302	1.3927635584023716
>1k	0.0013280741596610754	0.5590111431672005
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1056	0.5590111431672005	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	923	0.4886053836584527	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	601	0.31814933432148435	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	572	0.3027977025489002	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	535	0.2832111378735343	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	460	0.24350864190995475	No Hit
GAATCTATCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	396	0.20962917868770017	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	344	0.18210211481961833	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	324	0.17151478256266378	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	232	0.12281305418067281	No Hit
GCTGTCACTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	226	0.11963685450358645	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	210	0.11116698869802283	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	198	0.10481458934385009	TruSeq Adapter, Index 23 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09687409015113417	0.0
2	0.0	0.0	0.0	0.6511209338027051	0.0
3	0.0	0.0	0.0	0.7908737195945051	0.0
4	0.0	0.0	0.0	0.9930917657023371	0.0
5	0.0	0.0	0.0	1.7839654852968423	0.0
6	0.0	0.0	0.0	2.0613535904290514	0.0
7	0.0	0.0	0.0	2.461554749741934	0.0
8	0.0	0.0	0.0	3.247134803207962	0.0
9	0.0	0.0	0.0	3.469468780604007	0.0
10	0.0	0.0	0.0	5.0861544162409675	0.0
11	0.0	0.0	0.0	6.02683888727138	0.0
12	0.0	0.0	0.0	7.239088430692676	0.0
13	0.0	0.0	0.0	7.556708398401313	0.0
14	0.0	0.0	0.0	7.703872316772981	0.0
15	0.0	0.0	0.0	8.000317619967708	0.0
16	0.0	0.0	0.0	8.553505730393583	0.0
17	0.0	0.0	0.0	9.19721553161642	0.0
18	0.0	0.0	0.0	9.88433339509277	0.0
19	0.0	0.0	0.0	10.325295783594928	0.0
20	0.0	0.0	0.0	10.737143008390461	0.0
21	0.0	0.0	0.0	11.251157989465604	0.0
22	0.0	0.0	0.0	11.794288134247372	0.0
23	0.0	0.0	0.0	12.349064344511792	0.0
24	0.0	0.0	0.0	12.766205235435802	0.0
25	0.0	0.0	0.0	13.128291998623647	0.0
26	0.0	0.0	0.0	13.48190889600593	0.0
27	0.0	0.0	0.0	13.83605516000106	0.0
28	0.0	0.0	0.0	14.200259389640296	0.0
29	0.0	0.0	0.0	14.56499298589238	0.0
30	0.0	0.0	0.0	15.025541939069903	0.0
31	0.0	0.0	0.0	15.449564595960933	0.0
32	0.0	0.0	0.0	15.838649056404012	0.0
33	0.0	0.0	0.0	16.210793785235964	0.0
34	0.0	0.0	0.0	16.55911701648977	0.0
35	0.0	0.0	0.0	17.003255604669015	0.0
36	0.0	0.0	0.0	17.378047166565203	0.0
37	0.0	0.0	0.0	17.734840263624573	0.0
38	0.0	0.0	0.0	18.081046028426986	0.0
39	0.0	0.0	0.0	18.481776554352717	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACATTG	35	1.2047349E-7	45.000004	12
CGAATAT	70	0.0	45.000004	14
GCTACGA	70	0.0	45.000004	10
TTGGATC	20	7.018309E-4	45.0	36
ATCTAGC	20	7.018309E-4	45.0	15
GGTCGAT	25	3.878397E-5	45.0	43
GTTTCGT	20	7.018309E-4	45.0	42
GGGTCGA	25	3.878397E-5	45.0	42
CGGTTTA	20	7.018309E-4	45.0	1
ACCGAGT	20	7.018309E-4	45.0	11
GTCTATG	45	3.8198777E-10	45.0	11
TCGATTT	25	3.878397E-5	45.0	45
CCTGTCG	20	7.018309E-4	45.0	9
AGGCGAC	20	7.018309E-4	45.0	7
TATTGCC	20	7.018309E-4	45.0	24
TTATACG	25	3.878397E-5	45.0	4
GGAAGTT	20	7.018309E-4	45.0	8
AAACGGC	50	2.1827873E-11	45.0	6
GTAGAGT	40	6.7630026E-9	45.0	13
CGGGCAT	25	3.878397E-5	45.0	6
>>END_MODULE