FastQCFastQC Report
Sat 14 Jan 2017
SRR2934590.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934590.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615747
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58900.9565617047261294No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32390.5260277354173062No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12290.19959496351585962No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6190.10052830139651514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG207.030731E-445.0000041
ATCGCTA207.030731E-445.0000041
ATCGTTG351.2107012E-745.023
CGGTCTA302.1638534E-644.99999631
CGTTTTA13700.044.1788331
CGTTATT4700.043.0851061
CGTTTTT24400.042.0491831
GTTTTAT15400.040.3246772
AGGCGAT4150.040.120487
TTGCACG403.4547338E-739.3750041
TATACGG403.4547338E-739.3750042
CGAATAT1100.038.86363614
GCTACGA1100.038.86363610
ATACGGT356.2435647E-638.57142612
TGCGTAG356.2435647E-638.5714262
GACGTAA301.13930655E-437.4999961
CCCCCGT301.13930655E-437.49999623
TACGAAT1150.037.17391612
ACACGCG6950.036.58273336
TTACACG6900.036.5217434
Produced by FastQC (version 0.11.5)