##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934590.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	615747
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.888873189800357	31.0	31.0	34.0	30.0	34.0
2	32.08236174922492	33.0	31.0	34.0	30.0	34.0
3	31.674083673976487	33.0	31.0	34.0	30.0	34.0
4	35.604337495757186	37.0	35.0	37.0	33.0	37.0
5	32.00565491995901	37.0	35.0	37.0	0.0	37.0
6	33.90233163945582	37.0	35.0	37.0	19.0	37.0
7	20.856106485293473	32.0	0.0	37.0	0.0	37.0
8	28.15527156445748	35.0	17.0	37.0	17.0	37.0
9	34.89110300171986	37.0	32.0	39.0	32.0	39.0
10	36.302853282273404	37.0	35.0	39.0	32.0	39.0
11	36.74192484900454	38.0	37.0	39.0	33.0	39.0
12	37.088976478975944	39.0	37.0	39.0	34.0	39.0
13	36.923168119373706	39.0	37.0	39.0	33.0	39.0
14	38.282034666835564	40.0	38.0	41.0	34.0	41.0
15	38.45494172119393	40.0	38.0	41.0	34.0	41.0
16	38.51144707160571	40.0	38.0	41.0	34.0	41.0
17	38.474420500627694	40.0	38.0	41.0	34.0	41.0
18	38.328698312781064	40.0	38.0	41.0	34.0	41.0
19	38.22515741042994	40.0	37.0	41.0	34.0	41.0
20	38.14826381614527	40.0	37.0	41.0	34.0	41.0
21	38.037093156767305	40.0	37.0	41.0	33.0	41.0
22	37.995248048305555	40.0	36.0	41.0	34.0	41.0
23	37.90153098594065	40.0	36.0	41.0	33.0	41.0
24	37.75206537750083	40.0	36.0	41.0	33.0	41.0
25	37.61304074563092	39.0	35.0	41.0	33.0	41.0
26	37.48176442597366	39.0	35.0	41.0	33.0	41.0
27	37.424932642789976	39.0	35.0	41.0	33.0	41.0
28	37.33492327205817	39.0	35.0	41.0	33.0	41.0
29	37.29593485636146	39.0	35.0	41.0	33.0	41.0
30	37.124947421587116	39.0	35.0	41.0	32.0	41.0
31	36.9834493712515	39.0	35.0	41.0	32.0	41.0
32	36.799796020118656	39.0	35.0	41.0	31.0	41.0
33	36.55090808400203	39.0	35.0	41.0	30.0	41.0
34	36.234726275564476	39.0	35.0	41.0	29.0	41.0
35	35.95438873433407	39.0	35.0	41.0	27.0	41.0
36	35.708977875653474	39.0	35.0	41.0	26.0	41.0
37	35.580645947117894	39.0	35.0	40.0	25.0	41.0
38	35.41624563335266	39.0	35.0	40.0	24.0	41.0
39	35.29613461372934	39.0	35.0	40.0	24.0	41.0
40	35.18743250068616	39.0	35.0	40.0	23.0	41.0
41	35.034899073807914	38.0	34.0	40.0	23.0	41.0
42	34.92918682510837	38.0	34.0	40.0	23.0	41.0
43	34.8079454711107	38.0	34.0	40.0	22.0	41.0
44	34.67862937212849	38.0	34.0	40.0	22.0	41.0
45	34.560727051857334	38.0	34.0	40.0	22.0	41.0
46	34.44544918611053	38.0	34.0	40.0	21.0	41.0
47	34.38946352966397	38.0	34.0	40.0	20.0	41.0
48	34.28444312355562	38.0	33.0	40.0	20.0	41.0
49	34.172892437965594	38.0	33.0	40.0	20.0	41.0
50	34.00922619192623	37.0	33.0	40.0	20.0	41.0
51	32.320815204946186	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	17.0
9	38.0
10	46.0
11	37.0
12	21.0
13	14.0
14	21.0
15	35.0
16	83.0
17	145.0
18	267.0
19	480.0
20	873.0
21	1479.0
22	2468.0
23	4005.0
24	6409.0
25	9245.0
26	11087.0
27	11436.0
28	11471.0
29	11798.0
30	13181.0
31	16107.0
32	20897.0
33	29682.0
34	40022.0
35	52065.0
36	69555.0
37	115345.0
38	114439.0
39	72975.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.60254292753355	19.609514946885653	24.306898774983882	17.481043350596916
2	36.00082501416978	23.179974892285305	24.335319538706642	16.483880554838272
3	30.258693911622792	22.885373375753353	31.44960511378862	15.406327598835237
4	26.569516376044056	24.495125433010635	31.051227208577547	17.884130982367758
5	21.76007353669608	36.36623483346245	26.369596603799938	15.50409502604154
6	25.230329989427474	33.104018371181674	28.560431475914623	13.105220163476233
7	46.70619588889593	42.70065465199181	8.180795034324163	2.412354424788103
8	81.60786816663337	4.244113247811195	11.800301097691097	2.3477174878643337
9	75.65526100817381	7.121918580196088	13.445782115056994	3.7770382965731057
10	36.61341427566842	32.07145142404267	19.556408719815117	11.758725580473799
11	29.02442074423424	25.907393783485748	29.22547734702727	15.842708125252742
12	26.420429169772653	23.303727017752422	32.61566844824254	17.660175364232387
13	23.893417263908713	23.73101289977864	33.92416041003854	18.451409426274104
14	20.463761902209836	25.385913370264085	33.959564561418894	20.190760166107182
15	20.18085349989525	24.75156192397202	35.558273121915335	19.5093114542174
16	24.004014635881298	25.0565573198083	32.878763518133255	18.06066452617715
17	24.219200418353644	24.470439969662866	31.890208153673505	19.420151458309988
18	24.97941524684651	24.01310928027258	32.53462867054163	18.47284680233927
19	24.422205873516234	25.06760081656914	31.22532468692499	19.284868622989638
20	25.340115339579405	25.447464624269383	31.743719417228178	17.46870061892303
21	24.92354814558577	24.943686286737897	32.28095305377046	17.851812513905873
22	25.19541305113951	22.83616485342194	32.82078516013882	19.147636935299726
23	22.77412638632425	25.28457304704692	32.586922875791515	19.354377690837307
24	21.69494938667992	24.523058983641008	34.01364521467421	19.768346415004864
25	22.2537828036515	25.915838810420517	32.17327896035223	19.657099425575765
26	22.86003829494906	25.739630075339388	31.216392446897835	20.18393918281372
27	21.721096489304863	25.410598833611857	32.6640649487533	20.204239728329977
28	20.554383537394415	26.519495831891994	32.423544085476664	20.502576545236924
29	21.59036097618015	25.802642968621853	32.36930102785722	20.237695027340774
30	21.89454435019578	25.159846495395023	33.084042634393676	19.861566520015526
31	23.777785356648103	25.316241898052283	31.115864145501316	19.790108599798295
32	23.14554516708973	25.411086046704245	31.96426454371682	19.479104242489203
33	23.27611827585031	25.841457611648938	31.076724693745973	19.80569941875478
34	21.769005776723233	26.096757272061417	32.67332199750872	19.460914953706638
35	21.904938229500104	27.122340831542825	31.60535089898936	19.367370039967714
36	22.94530058611735	27.83009905042168	29.655361698879574	19.569238664581395
37	22.413101484863102	27.68767042307961	29.786097212004282	20.11313088005301
38	22.36616662362951	28.29327629692065	29.326330457152043	20.014226622297794
39	22.616918961846345	26.79996816874463	29.80477371387924	20.77833915552979
40	24.227807849652535	26.41831791303896	29.725520384183763	19.628353853124743
41	21.60968709551163	26.560259327288644	30.324630083459603	21.505423493740125
42	22.918666270400017	26.161556613349312	29.67436300948279	21.245414106767875
43	22.541238528161728	26.45810698225083	29.40509657375513	21.595557915832313
44	22.226336466113516	27.012555481390898	29.64707907630894	21.114028976186646
45	22.428042686363067	26.752221285690393	29.344519745934612	21.47521628201193
46	21.80944446339162	27.559208571052725	29.02360872241359	21.60773824314207
47	21.813179763766612	27.218159406379566	30.220366481688092	20.748294348165725
48	21.71979725439182	27.20110694814591	30.563851711823197	20.51524408563907
49	22.575343444629045	26.683199430935105	29.76011251374347	20.98134461069238
50	21.233558588186384	27.539720047357115	30.37140254032906	20.85531882412744
51	21.630799662848542	26.720714839049155	29.53437044760267	22.114115050499635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	359.0
1	864.5
2	1370.0
3	8840.0
4	16310.0
5	11154.5
6	5999.0
7	5237.0
8	4475.0
9	4347.5
10	4220.0
11	4144.5
12	4069.0
13	3972.0
14	3875.0
15	3643.5
16	3412.0
17	3330.0
18	3248.0
19	3256.0
20	3264.0
21	3299.0
22	3334.0
23	3723.5
24	4113.0
25	4729.0
26	6266.0
27	7187.0
28	8518.0
29	9849.0
30	11750.0
31	13651.0
32	15399.5
33	17148.0
34	19104.5
35	21061.0
36	23015.5
37	24970.0
38	26506.0
39	28042.0
40	29918.0
41	31794.0
42	33404.0
43	35014.0
44	36373.0
45	37732.0
46	38661.0
47	39590.0
48	39548.5
49	39507.0
50	39200.5
51	38894.0
52	37810.5
53	36727.0
54	35152.5
55	33578.0
56	31785.5
57	29993.0
58	28671.0
59	27349.0
60	25087.0
61	22825.0
62	20449.5
63	18074.0
64	15790.5
65	13507.0
66	11710.5
67	9914.0
68	8274.0
69	6634.0
70	5330.0
71	4026.0
72	3179.0
73	2332.0
74	1879.0
75	1064.5
76	703.0
77	539.0
78	375.0
79	297.0
80	219.0
81	153.5
82	88.0
83	71.0
84	54.0
85	45.5
86	37.0
87	29.5
88	22.0
89	19.0
90	16.0
91	13.5
92	11.0
93	7.0
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	615747.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.91018811606584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.37839706623848	28.886354424528854
2	7.696633648265201	6.143481935254126
3	4.011379558307177	4.802847383309416
4	2.79151206141502	4.456390859973607
5	2.061422026749813	4.113587043709337
6	1.7416368799553852	4.170543330533843
7	1.467892108929757	4.100869514103265
8	1.2701164485871719	4.05524691139388
9	1.0066214811444685	3.61570074067337
>10	5.539276939291605	32.49316240246411
>50	0.02587183871812185	0.6706043158161509
>100	0.007391953918236402	0.4672288523647585
>500	4.6199711988977515E-4	0.11413364625642679
>1k	9.239942397795503E-4	0.8238273529462276
>5k	4.6199711988977515E-4	1.086021286672623
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5890	0.9565617047261294	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3239	0.5260277354173062	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1229	0.19959496351585962	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	619	0.10052830139651514	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.872130923902187E-4	0.0	0.0	0.01234273167388554	0.0
2	4.872130923902187E-4	0.0	0.0	0.05148218342923311	0.0
3	4.872130923902187E-4	0.0	0.0	0.08022775588025602	0.0
4	4.872130923902187E-4	0.0	0.0	0.10280196249433615	0.0
5	4.872130923902187E-4	0.0	0.0	0.17637113944525917	0.0
6	4.872130923902187E-4	0.0	0.0	0.2830708066787171	0.0
7	4.872130923902187E-4	0.0	0.0	0.37434205931981807	0.0
8	4.872130923902187E-4	0.0	0.0	0.5844933065041323	0.0
9	4.872130923902187E-4	0.0	0.0	0.6944410610201918	0.0
10	4.872130923902187E-4	0.0	0.0	0.8812060797697756	0.0
11	4.872130923902187E-4	0.0	0.0	1.0791769996443343	0.0
12	4.872130923902187E-4	0.0	0.0	1.2438550248722284	0.0
13	4.872130923902187E-4	0.0	0.0	1.3328526164155083	0.0
14	4.872130923902187E-4	0.0	0.0	1.3861212478501723	0.0
15	4.872130923902187E-4	0.0	0.0	1.4566047418826238	0.0
16	4.872130923902187E-4	0.0	0.0	1.556808234550879	0.0
17	4.872130923902187E-4	0.0	0.0	1.6854324909418965	0.0
18	4.872130923902187E-4	0.0	0.0	1.8257498615502796	0.0
19	4.872130923902187E-4	0.0	0.0	1.9103625352620475	0.0
20	4.872130923902187E-4	0.0	0.0	2.009429197381392	0.0
21	4.872130923902187E-4	0.0	0.0	2.1348053664898083	0.0
22	4.872130923902187E-4	0.0	0.0	2.276584376375362	0.0
23	4.872130923902187E-4	0.0	0.0	2.4268084131956793	0.0
24	4.872130923902187E-4	0.0	0.0	2.5387050200812995	0.0
25	4.872130923902187E-4	0.0	0.0	2.655311353526692	0.0
26	4.872130923902187E-4	0.0	0.0	2.7545404200101666	0.0
27	4.872130923902187E-4	0.0	0.0	2.8927465338848584	0.0
28	4.872130923902187E-4	0.0	0.0	3.0155242331671936	0.0
29	4.872130923902187E-4	0.0	0.0	3.143174063373431	0.0
30	4.872130923902187E-4	0.0	0.0	3.3098009409708857	0.0
31	4.872130923902187E-4	0.0	0.0	3.4470326286607973	0.0
32	4.872130923902187E-4	0.0	0.0	3.6008295614919765	0.0
33	4.872130923902187E-4	0.0	0.0	3.7393604840949286	0.0
34	4.872130923902187E-4	0.0	0.0	3.902252061317392	0.0
35	4.872130923902187E-4	0.0	0.0	4.053612928686619	0.0
36	4.872130923902187E-4	0.0	0.0	4.205948222240628	0.0
37	6.496174565202916E-4	0.0	0.0	4.36591652090875	0.0
38	8.120218206503645E-4	0.0	0.0	4.562588205870268	0.0
39	8.120218206503645E-4	0.0	0.0	4.8336410896033595	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	20	7.030731E-4	45.000004	1
ATCGCTA	20	7.030731E-4	45.000004	1
ATCGTTG	35	1.2107012E-7	45.0	23
CGGTCTA	30	2.1638534E-6	44.999996	31
CGTTTTA	1370	0.0	44.178833	1
CGTTATT	470	0.0	43.085106	1
CGTTTTT	2440	0.0	42.049183	1
GTTTTAT	1540	0.0	40.324677	2
AGGCGAT	415	0.0	40.12048	7
TTGCACG	40	3.4547338E-7	39.375004	1
TATACGG	40	3.4547338E-7	39.375004	2
CGAATAT	110	0.0	38.863636	14
GCTACGA	110	0.0	38.863636	10
ATACGGT	35	6.2435647E-6	38.571426	12
TGCGTAG	35	6.2435647E-6	38.571426	2
GACGTAA	30	1.13930655E-4	37.499996	1
CCCCCGT	30	1.13930655E-4	37.499996	23
TACGAAT	115	0.0	37.173916	12
ACACGCG	695	0.0	36.582733	36
TTACACG	690	0.0	36.52174	34
>>END_MODULE