##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934588.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	622375
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.672657160072305	31.0	31.0	34.0	30.0	34.0
2	31.84821209078128	31.0	31.0	34.0	30.0	34.0
3	31.007500301265313	31.0	31.0	34.0	27.0	34.0
4	35.1982679252862	37.0	35.0	37.0	33.0	37.0
5	31.657920064269934	37.0	35.0	37.0	0.0	37.0
6	33.707258485639684	37.0	35.0	37.0	17.0	37.0
7	21.28340791323559	32.0	0.0	37.0	0.0	37.0
8	28.40683832094798	35.0	17.0	37.0	17.0	37.0
9	35.00396384816228	37.0	32.0	39.0	32.0	39.0
10	36.30608716609761	37.0	35.0	39.0	32.0	39.0
11	36.73600321349669	39.0	37.0	39.0	33.0	39.0
12	37.14658043783892	39.0	37.0	39.0	34.0	39.0
13	36.9935119501908	39.0	37.0	39.0	33.0	39.0
14	38.38766177947379	40.0	38.0	41.0	34.0	41.0
15	38.54496404900583	40.0	38.0	41.0	34.0	41.0
16	38.607265716007234	40.0	38.0	41.0	34.0	41.0
17	38.589925687889135	40.0	38.0	41.0	34.0	41.0
18	38.387019080136575	40.0	38.0	41.0	34.0	41.0
19	38.20913597107853	40.0	37.0	41.0	34.0	41.0
20	37.99680899779072	40.0	36.0	41.0	34.0	41.0
21	37.889934525005025	40.0	36.0	41.0	33.0	41.0
22	37.78560674834304	39.0	35.0	41.0	33.0	41.0
23	37.650745932918255	39.0	35.0	41.0	33.0	41.0
24	37.4525133560956	39.0	35.0	41.0	33.0	41.0
25	37.297476601727254	39.0	35.0	41.0	33.0	41.0
26	37.176155854589275	39.0	35.0	41.0	33.0	41.0
27	37.11797228359108	39.0	35.0	41.0	33.0	41.0
28	37.03586583651336	39.0	35.0	41.0	33.0	41.0
29	36.990359509941754	39.0	35.0	41.0	33.0	41.0
30	36.79918537859008	39.0	35.0	41.0	32.0	41.0
31	36.571719622414136	39.0	35.0	40.0	31.0	41.0
32	36.29945772243423	39.0	35.0	40.0	30.0	41.0
33	35.85413456517373	39.0	35.0	40.0	28.0	41.0
34	35.362116087567784	38.0	34.0	40.0	24.0	41.0
35	34.92614420566379	38.0	34.0	40.0	21.0	41.0
36	34.578827877083754	38.0	34.0	40.0	19.0	41.0
37	34.40517212291625	38.0	34.0	40.0	18.0	41.0
38	34.20102349869452	38.0	33.0	40.0	16.0	41.0
39	34.055336412934324	38.0	33.0	40.0	15.0	41.0
40	33.96599638481623	38.0	33.0	40.0	15.0	41.0
41	33.821059650532234	38.0	33.0	40.0	15.0	41.0
42	33.69756818638281	38.0	33.0	40.0	14.0	41.0
43	33.60319903595099	38.0	33.0	40.0	13.0	41.0
44	33.44532797750552	38.0	33.0	40.0	12.0	41.0
45	33.36835669813215	38.0	33.0	40.0	12.0	41.0
46	33.248297248443464	38.0	33.0	40.0	10.0	41.0
47	33.2096276360715	38.0	33.0	40.0	10.0	41.0
48	33.085075718015666	38.0	33.0	40.0	10.0	41.0
49	32.991503514762	37.0	32.0	40.0	10.0	41.0
50	32.82791243221531	37.0	32.0	40.0	10.0	41.0
51	31.329150431813616	35.0	28.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	33.0
10	25.0
11	21.0
12	19.0
13	13.0
14	24.0
15	37.0
16	61.0
17	111.0
18	269.0
19	525.0
20	1051.0
21	1920.0
22	3555.0
23	6148.0
24	10045.0
25	14446.0
26	16982.0
27	16479.0
28	15083.0
29	13983.0
30	14511.0
31	16608.0
32	20717.0
33	29189.0
34	38387.0
35	52150.0
36	62475.0
37	104703.0
38	109770.0
39	73012.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.01895963044788	17.601446073508736	22.52098815023097	23.85860614581241
2	42.21940148624222	20.54822253464551	22.44675637678249	14.785619602329787
3	28.13480618598112	20.91745330387628	36.972564772042574	13.97517573810002
4	23.19052018477606	22.83591082546696	37.71664992970476	16.25691906005222
5	19.108736694115283	34.48596103635268	32.5123920465957	13.892910222936333
6	21.529303072906206	31.217031532436234	35.798513757782686	11.455151636874875
7	43.0308093994778	40.958746736292426	13.746856798553928	2.263587065675839
8	75.02920265113477	3.6307692307692303	19.13573006627837	2.2042980518176343
9	70.44723840128539	6.176983329985941	20.228640289214702	3.1471379795139587
10	32.1261297449287	28.85800361518377	27.585619602329785	11.430247037557741
11	23.409519983932515	24.984454709781083	36.81703153243623	14.788993773850171
12	22.429564169511952	22.249929704759992	39.133159268929504	16.187346856798555
13	20.20341434022896	23.83402289616389	39.359710785298255	16.602851978308898
14	16.743442458324964	25.202490459931713	40.21964249849368	17.834424583249646
15	16.624061056437036	25.50118497690299	41.94175537256477	15.9329985940952
16	19.794978911428	24.74006828680458	40.15649728861217	15.308455513155254
17	19.97718417352882	24.447158063868248	38.4867644105242	17.08889335207873
18	20.172725446876882	24.019280980116488	39.428800964049	16.37919260895762
19	19.68989756979313	26.31146816629845	37.41827676240209	16.580357501506327
20	19.791283390239002	26.253143201446072	38.380076320546294	15.575497087768628
21	19.476521389837316	25.894998995782288	39.572604940751155	15.055874673629244
22	19.386382807792728	24.10765213898373	39.23181361719221	17.27415143603133
23	17.183370154649527	25.63615183771842	39.99084153444466	17.189636473187385
24	17.124081140791322	25.2609760996184	40.70214902590882	16.91279373368146
25	17.400120506125727	27.002851978308897	38.72793733681462	16.869090178750753
26	17.786222132958425	27.082707370958026	37.56384816228158	17.56722233380197
27	16.78473589074111	26.514721831693112	39.60666800562362	17.09387427194216
28	16.223980719019885	26.664149427595902	39.3654950793332	17.746374774051013
29	16.565575416750352	25.613818035750153	39.855874673629245	17.964731873870253
30	17.575256075517174	26.27628037758586	38.57240409720827	17.576059449688692
31	18.011327575818438	26.411889937738504	37.89821249246837	17.678569993974694
32	18.290259088170316	26.00875677846957	37.69270937939345	18.00827475396666
33	18.427314721831692	26.95240008033742	36.65330387628038	17.966981321550513
34	16.80755171721229	27.368065876682063	37.81819642498493	18.006185981120705
35	17.46390841534445	27.580317332797748	36.529102229363325	18.42667202249448
36	18.234022896163886	28.778951596706165	34.786422976501306	18.20060253062864
37	18.080578429403495	29.551797549708773	34.89327174131351	17.474352279574212
38	18.208314922675235	29.48077927294637	34.2936332596907	18.01727254468769
39	18.082988551918056	29.288933520787307	33.675838521791526	18.952239405503114
40	19.552520586463146	28.719019883510743	33.6040168708576	18.12444265916851
41	17.237196224141393	28.84016870857602	34.12299658565977	19.799638481622818
42	18.162201245229966	29.15027113878289	33.484474794135366	19.203052821851777
43	18.698533842136975	28.554167503514762	33.66651938140189	19.080779272946373
44	18.546374774051014	28.61522394055031	32.89254870455915	19.945852580839528
45	18.422333801968264	29.193171319542078	32.2125728057843	20.17192207270536
46	18.237397067684274	29.483189395460936	32.53713597107853	19.74227756577626
47	17.888732677244427	29.514681662984536	33.492508535850575	19.104077123920465
48	17.80052219321149	29.117975497087766	33.623458525808395	19.458043783892347
49	18.40482024502912	28.516087567784698	33.44478811006226	19.63430407712392
50	17.267885117493474	29.283791926089574	33.2376782486443	20.210644707772644
51	16.879052018477605	29.130026109660573	33.42839927696325	20.562522594898574
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	614.0
1	1011.5
2	1409.0
3	18359.0
4	35309.0
5	23814.0
6	12319.0
7	10580.0
8	8841.0
9	8724.0
10	8607.0
11	8486.0
12	8365.0
13	8244.5
14	8124.0
15	7839.5
16	7555.0
17	7192.0
18	6829.0
19	6449.0
20	6069.0
21	6083.5
22	6098.0
23	6217.0
24	6336.0
25	6834.0
26	8622.0
27	9912.0
28	11172.5
29	12433.0
30	13913.0
31	15393.0
32	17416.5
33	19440.0
34	21503.5
35	23567.0
36	24644.0
37	25721.0
38	27872.0
39	30023.0
40	32369.0
41	34715.0
42	36472.5
43	38230.0
44	39478.5
45	40727.0
46	42021.5
47	43316.0
48	42681.0
49	42046.0
50	39790.0
51	37534.0
52	34769.5
53	32005.0
54	29024.0
55	26043.0
56	23589.0
57	21135.0
58	18580.5
59	16026.0
60	13681.5
61	11337.0
62	9674.5
63	8012.0
64	6321.0
65	4630.0
66	3631.5
67	2633.0
68	2099.5
69	1566.0
70	1253.5
71	941.0
72	723.0
73	505.0
74	393.0
75	207.0
76	133.0
77	118.0
78	103.0
79	91.0
80	79.0
81	56.0
82	33.0
83	25.0
84	17.0
85	15.0
86	13.0
87	10.5
88	8.0
89	6.5
90	5.0
91	3.5
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	622375.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.69452155695288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.31722254066923	34.55656269567664
2	8.08863967119955	7.23035760301705
3	3.5427962599375666	4.750307514350148
4	2.0032218409806766	3.5813216702027875
5	1.3961617603831142	3.1200390948218186
6	1.0705032873864586	2.8707379352929796
7	0.8519525192369636	2.665432716557574
8	0.7382333835289374	2.6395990299357086
9	0.6171969753305575	2.4826791166917945
>10	4.335363027670129	30.23535561974654
>50	0.025539783056096308	0.797258293196236
>100	0.011572714181839224	0.8734169704295353
>500	3.9905910971859386E-4	0.11414883828362317
>1k	3.9905910971859386E-4	0.2641475460906967
>5k	3.9905910971859386E-4	1.3845897368683853
>10k+	3.9905910971859386E-4	2.4340456188384456
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13647	2.1927294637477406	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7763	1.2473187387025508	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1481	0.23795942960433822	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	640	0.10283189395460936	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02345852580839526	0.0
2	0.0	0.0	0.0	0.09158465555332396	0.0
3	0.0	0.0	0.0	0.14508937537658165	0.0
4	0.0	0.0	0.0	0.20004016870857602	0.0
5	0.0	0.0	0.0	0.3512351877887126	0.0
6	0.0	0.0	0.0	0.585338421369753	0.0
7	0.0	0.0	0.0	0.7938943562964451	0.0
8	0.0	0.0	0.0	1.2201646917051616	0.0
9	0.0	0.0	0.0	1.4378790921871862	0.0
10	0.0	0.0	0.0	1.756336613777867	0.0
11	0.0	0.0	0.0	2.110785298252661	0.0
12	0.0	0.0	0.0	2.3738100020084354	0.0
13	0.0	0.0	0.0	2.508937537658164	0.0
14	0.0	0.0	0.0	2.582044587266519	0.0
15	0.0	0.0	0.0	2.668648322956417	0.0
16	0.0	0.0	0.0	2.829483832094798	0.0
17	0.0	0.0	0.0	3.0406105643703554	0.0
18	0.0	0.0	0.0	3.2732677244426593	0.0
19	0.0	0.0	0.0	3.4305683872263506	0.0
20	0.0	0.0	0.0	3.599276963245632	0.0
21	0.0	0.0	0.0	3.7964249849367344	0.0
22	0.0	0.0	0.0	4.013818035750151	0.0
23	0.0	0.0	0.0	4.258043783892348	0.0
24	0.0	0.0	0.0	4.440249045993172	0.0
25	0.0	0.0	0.0	4.622936332596907	0.0
26	0.0	0.0	0.0	4.780558345049207	0.0
27	0.0	0.0	0.0	4.982365936935127	0.0
28	0.0	0.0	0.0	5.169391444065074	0.0
29	0.0	0.0	0.0	5.378750753163286	0.0
30	0.0	0.0	0.0	5.644988953605141	0.0
31	1.6067483430407713E-4	0.0	0.0	5.873468567985539	0.0
32	1.6067483430407713E-4	0.0	0.0	6.095681863828078	0.0
33	1.6067483430407713E-4	0.0	0.0	6.312432215304278	0.0
34	1.6067483430407713E-4	0.0	0.0	6.5304679654549105	0.0
35	1.6067483430407713E-4	0.0	0.0	6.799758987748544	0.0
36	1.6067483430407713E-4	0.0	0.0	7.024221731271339	0.0
37	1.6067483430407713E-4	0.0	0.0	7.261699136372766	0.0
38	1.6067483430407713E-4	0.0	0.0	7.535489054026913	0.0
39	1.6067483430407713E-4	0.0	0.0	7.91211086563567	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTTCG	45	3.8380676E-10	45.000004	23
CTCAACG	20	7.0307904E-4	45.0	29
GCGCGAA	20	7.0307904E-4	45.0	1
CTCGCTA	20	7.0307904E-4	45.0	29
TAGTGCG	20	7.0307904E-4	45.0	1
TCGACGT	20	7.0307904E-4	45.0	26
ACGGGTC	25	3.888738E-5	44.999996	5
GCGCAGT	25	3.888738E-5	44.999996	36
CTAGCGG	30	2.1638916E-6	44.999996	2
TAATCCG	25	3.888738E-5	44.999996	31
CGTTATT	1170	0.0	44.23077	1
CGTTTTA	2495	0.0	44.188377	1
CGGTTTA	215	0.0	43.95349	1
CGTTTTT	5110	0.0	43.458904	1
AGGCGAT	275	0.0	43.363636	7
ATCGTTG	85	0.0	42.35294	23
CGGTTTT	480	0.0	40.781246	1
CGTTGTT	50	1.0804797E-9	40.499996	1
GTTTTAT	2775	0.0	40.216213	2
AATCGTT	90	0.0	40.000004	22
>>END_MODULE