##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934587.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	541545
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57481834381261	31.0	31.0	34.0	30.0	34.0
2	31.763125871349565	31.0	31.0	34.0	30.0	34.0
3	31.257838222123738	31.0	31.0	34.0	28.0	34.0
4	35.27290991515017	37.0	35.0	37.0	33.0	37.0
5	31.685926377309364	35.0	35.0	37.0	0.0	37.0
6	33.685725101330455	37.0	35.0	37.0	17.0	37.0
7	21.227307056661957	32.0	0.0	37.0	0.0	37.0
8	28.372044797754572	35.0	17.0	37.0	17.0	37.0
9	34.890254734140285	37.0	32.0	39.0	32.0	39.0
10	36.084419577320446	37.0	35.0	39.0	32.0	39.0
11	36.473942147005324	38.0	35.0	39.0	32.0	39.0
12	36.9619588399856	39.0	37.0	39.0	33.0	39.0
13	36.8072016175941	39.0	37.0	39.0	33.0	39.0
14	38.19168490153173	40.0	37.0	41.0	33.0	41.0
15	38.345252933735885	40.0	38.0	41.0	34.0	41.0
16	38.373714095781516	40.0	38.0	41.0	34.0	41.0
17	38.33448189901116	40.0	38.0	41.0	34.0	41.0
18	38.19589692453997	40.0	37.0	41.0	34.0	41.0
19	38.08353691752301	40.0	37.0	41.0	34.0	41.0
20	37.9765873565447	40.0	36.0	41.0	34.0	41.0
21	37.873626383772354	39.0	36.0	41.0	33.0	41.0
22	37.830481308109206	39.0	36.0	41.0	33.0	41.0
23	37.742196862679926	39.0	36.0	41.0	33.0	41.0
24	37.602452243119224	39.0	35.0	41.0	33.0	41.0
25	37.45102623050716	39.0	35.0	41.0	33.0	41.0
26	37.32959218532162	39.0	35.0	41.0	33.0	41.0
27	37.29320555078525	39.0	35.0	41.0	33.0	41.0
28	37.22939737233286	39.0	35.0	41.0	33.0	41.0
29	37.233461669852	39.0	35.0	41.0	33.0	41.0
30	37.045937087407324	39.0	35.0	40.0	32.0	41.0
31	36.85074739864646	39.0	35.0	40.0	31.0	41.0
32	36.66942174703856	39.0	35.0	40.0	31.0	41.0
33	36.34981765134938	39.0	35.0	40.0	30.0	41.0
34	36.008789666602034	39.0	35.0	40.0	28.0	41.0
35	35.691772613540884	39.0	35.0	40.0	25.0	41.0
36	35.40123904753991	39.0	35.0	40.0	24.0	41.0
37	35.241160014403235	39.0	35.0	40.0	24.0	41.0
38	35.13027541570876	39.0	35.0	40.0	23.0	41.0
39	35.00475676074934	39.0	35.0	40.0	22.0	41.0
40	34.92821095199845	39.0	34.0	40.0	22.0	41.0
41	34.82777977822711	39.0	34.0	40.0	21.0	41.0
42	34.72667091377448	38.0	34.0	40.0	21.0	41.0
43	34.61713800330536	38.0	34.0	40.0	20.0	41.0
44	34.479928722451504	38.0	34.0	40.0	18.0	41.0
45	34.38861221135824	38.0	34.0	40.0	19.0	41.0
46	34.20198690782853	38.0	33.0	40.0	18.0	41.0
47	34.15703588806101	38.0	33.0	40.0	18.0	41.0
48	34.01934280623032	38.0	33.0	40.0	17.0	41.0
49	33.880748598916064	38.0	33.0	40.0	15.0	41.0
50	33.663852496099125	38.0	33.0	40.0	15.0	41.0
51	31.712600060936765	36.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	19.0
10	19.0
11	19.0
12	12.0
13	5.0
14	5.0
15	12.0
16	23.0
17	62.0
18	153.0
19	351.0
20	647.0
21	1333.0
22	2308.0
23	4183.0
24	6717.0
25	9534.0
26	11441.0
27	11269.0
28	11071.0
29	11267.0
30	12596.0
31	15100.0
32	19351.0
33	26016.0
34	35272.0
35	46458.0
36	60427.0
37	95482.0
38	97447.0
39	62930.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.112973067796766	20.641497936459572	25.95647637777101	17.289052617972654
2	36.53029757453213	22.91185404721676	25.495388194886853	15.062460183364262
3	28.537609986243062	22.260569297103658	34.51919969716275	14.682621019490533
4	23.637001541884793	24.307490605582178	35.04233258547305	17.013175267059985
5	19.877941814622975	35.37434562224746	29.460524979456927	15.28718758367264
6	22.779639734463434	31.95819368658191	32.253644664801634	13.008521914153025
7	47.09562455566943	40.752661367014746	9.731416595112133	2.420297482203695
8	80.96058499293687	3.014338605286726	13.17194323648081	2.8531331652955894
9	75.33759890683139	6.569352500715546	14.793230479461542	3.2998181129915336
10	31.244494917319894	37.39227580348817	21.030200629679896	10.333028649512045
11	21.981368122686018	26.111957455059137	36.30557017422375	15.601104248031097
12	22.038981063438865	22.71722571531452	37.754572565530104	17.489220655716515
13	19.807033579850245	25.49040245962939	38.30115687523659	16.401407085283772
14	17.58912001772706	28.328024448568446	35.897478510557754	18.18537702314674
15	16.155998116500015	27.545448670008955	38.95096437045859	17.347588843032437
16	19.71525911974074	26.567875245824446	36.17335586147043	17.543509772964388
17	19.8513512265832	25.965893877701763	34.68280567635192	19.49994921936312
18	19.66503245344339	25.254226333915003	36.622441348364404	18.458299864277205
19	18.92585103730992	28.504187094331957	33.961166662050246	18.608795206307878
20	19.701963825720853	27.35654470080972	34.97899528201719	17.962496191452235
21	19.26229583875763	27.059801124560284	36.51164723153201	17.16625580515008
22	19.343175544045277	24.361041095384504	35.10308469286947	21.192698667700746
23	16.92860242454459	27.25424479960114	35.49381861156506	20.323334164289207
24	17.20374114801171	26.149442797920763	37.48183438126102	19.16498167280651
25	17.0100361004164	28.720789592739294	34.699609450738166	19.56956485610614
26	17.146682177843022	28.82807522920533	34.66895641174787	19.356286181203778
27	17.57120830217249	27.54009362102872	35.54459924844657	19.344098828352216
28	15.782252629052065	26.718370587855116	37.1134439427933	20.385932840299514
29	16.395682722580766	24.829146239001375	36.724002622127436	22.05116841629043
30	18.181683885918993	26.47628544257633	35.30048287769253	20.041547793812146
31	18.14863030773066	27.598075875504346	34.54634425578668	19.706949560978313
32	18.015308053809008	27.02933274243138	34.419854305736365	20.535504898023248
33	18.156570552770315	26.86960455733134	34.07085283771431	20.90297205218403
34	16.898318699277066	26.73886749946911	34.29798077722073	22.06483302403309
35	16.63093556398822	26.390973972615388	34.291148473349395	22.686941990046996
36	18.951149027319982	26.855939949588674	33.206104755837465	20.986806267253876
37	16.92934105199014	28.09775734241845	34.33251161030016	20.64038999529125
38	17.44785751876575	28.159063420399043	33.00224358086586	21.390835479969347
39	18.276782169533465	28.11566905797302	32.105549861968996	21.501998910524517
40	19.569195542383365	27.166902104164937	32.16297814586046	21.10092420759124
41	17.397261538745624	27.02637823264918	32.373856281564784	23.202503947040412
42	17.76362075173808	26.830457302717225	32.54595647637777	22.85996546916692
43	18.122778347136435	26.815869410667624	32.6722617695667	22.389090472629235
44	18.154354670433666	26.899703625737477	31.533667562252443	23.412274141576418
45	18.63353922573378	26.977628821242927	30.483523991542715	23.905307961480577
46	19.490531719432365	27.682094747435578	30.841388988911355	21.985984544220702
47	17.292561098339014	27.215466858709803	32.854702748617385	22.6372692943338
48	17.67867859549991	25.944289024919442	33.192070834372025	23.184961545208616
49	18.400317609801586	25.346739421470055	33.018308727806556	23.234634240921807
50	17.985762955987035	25.680229713135567	32.647702407002186	23.68630492387521
51	17.917070603550954	25.752799859660787	31.046542761912676	25.283586774875587
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	390.0
1	578.0
2	766.0
3	7421.0
4	14076.0
5	10231.0
6	6386.0
7	5631.0
8	4876.0
9	4712.5
10	4549.0
11	4530.5
12	4512.0
13	4351.0
14	4190.0
15	4042.5
16	3895.0
17	3763.0
18	3631.0
19	3517.5
20	3404.0
21	3518.0
22	3632.0
23	3877.0
24	4122.0
25	4621.5
26	6281.0
27	7441.0
28	8724.0
29	10007.0
30	11441.5
31	12876.0
32	15187.5
33	17499.0
34	19944.0
35	22389.0
36	24132.5
37	25876.0
38	27952.5
39	30029.0
40	32640.0
41	35251.0
42	38231.5
43	41212.0
44	44146.0
45	47080.0
46	50231.5
47	53383.0
48	51913.0
49	50443.0
50	45668.0
51	40893.0
52	34671.0
53	28449.0
54	23725.5
55	19002.0
56	15757.0
57	12512.0
58	10628.0
59	8744.0
60	7377.5
61	6011.0
62	4952.5
63	3894.0
64	3043.0
65	2192.0
66	1714.0
67	1236.0
68	962.5
69	689.0
70	535.5
71	382.0
72	289.5
73	197.0
74	171.5
75	123.5
76	101.0
77	59.5
78	18.0
79	20.0
80	22.0
81	17.5
82	13.0
83	8.0
84	3.0
85	3.0
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	541545.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.26550829815038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.29171930692065	42.76779241015544
2	8.099476863653676	8.628455041832437
3	3.0788929577526845	4.9199639517087705
4	1.7848990804977494	3.8029422713445538
5	1.2365513582556946	3.2932768317128907
6	0.9138137656409621	2.9204852830027628
7	0.7275082879850155	2.712576912544731
8	0.5913525527621826	2.5198955445029156
9	0.49730940042598276	2.3840494195624533
>10	2.746567139449275	22.45597452874847
>50	0.018993623025807753	0.657608239926345
>100	0.011776046251195821	1.1974540175971675
>500	3.798724597159943E-4	0.1284865328107809
>1k	3.798724597159943E-4	0.5590681734743111
>5k	3.798724597159943E-4	1.051970841075984
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5199	0.960031022352713	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2763	0.5102069080131845	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	635	0.11725710698095265	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09103583266395221	0.0
2	0.0	0.0	0.0	0.4138160263689998	0.0
3	0.0	0.0	0.0	0.5637573978155094	0.0
4	0.0	0.0	0.0	0.7238548966383218	0.0
5	0.0	0.0	0.0	1.1862356775521887	0.0
6	0.0	0.0	0.0	1.6395682722580764	0.0
7	0.0	0.0	0.0	2.0637250828647664	0.0
8	0.0	0.0	0.0	3.056809683405811	0.0
9	0.0	0.0	0.0	3.4274160042101762	0.0
10	0.0	0.0	0.0	4.308044576166339	0.0
11	0.0	0.0	0.0	5.4113693229556175	0.0
12	0.0	0.0	0.0	6.27334755191166	0.0
13	0.0	0.0	0.0	6.663896813745857	0.0
14	0.0	0.0	0.0	6.8422753418460145	0.0
15	0.0	0.0	0.0	7.1050420556001805	0.0
16	0.0	0.0	0.0	7.700560433574311	0.0
17	0.0	0.0	0.0	8.489045231698197	0.0
18	0.0	0.0	0.0	9.363580127228577	0.0
19	0.0	0.0	0.0	9.916996740806397	0.0
20	0.0	0.0	0.0	10.437175119334496	0.0
21	0.0	0.0	0.0	11.116343055517085	0.0
22	0.0	0.0	0.0	11.857186383403041	0.0
23	0.0	0.0	0.0	12.680755985190519	0.0
24	0.0	0.0	0.0	13.291231568937024	0.0
25	0.0	0.0	0.0	13.831537545356342	0.0
26	0.0	0.0	0.0	14.333065580884321	0.0
27	0.0	0.0	0.0	14.858414351531268	0.0
28	0.0	0.0	0.0	15.413677533722959	0.0
29	0.0	0.0	0.0	16.014920274400097	0.0
30	0.0	0.0	0.0	16.666389681374586	0.0
31	0.0	0.0	0.0	17.271140902418082	0.0
32	0.0	0.0	0.0	17.855949182431747	0.0
33	1.84656861387327E-4	0.0	0.0	18.408996482286792	0.0
34	1.84656861387327E-4	0.0	0.0	18.943208782280326	0.0
35	1.84656861387327E-4	0.0	0.0	19.52746309170983	0.0
36	1.84656861387327E-4	0.0	0.0	20.104885097268003	0.0
37	3.69313722774654E-4	0.0	0.0	20.691355289034153	0.0
38	3.69313722774654E-4	0.0	0.0	21.243848618305034	0.0
39	3.69313722774654E-4	0.0	0.0	21.823117192477078	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	20	7.0299796E-4	45.0	1
TCGCACA	20	7.0299796E-4	45.0	1
TAGCGAA	20	7.0299796E-4	45.0	1
CGTTCTA	20	7.0299796E-4	45.0	1
CGACAAT	25	3.888063E-5	45.0	20
CATCGAC	25	3.888063E-5	45.0	17
TGTAGCG	20	7.0299796E-4	45.0	1
ACCCGCT	20	7.0299796E-4	45.0	12
TTCATCG	25	3.888063E-5	45.0	15
AAGTGCG	20	7.0299796E-4	45.0	1
CGTTTTA	1305	0.0	44.482758	1
CGTTTTT	2560	0.0	43.945312	1
CGAAGGA	145	0.0	43.44828	4
CGTTATT	575	0.0	43.434784	1
CGGTTTA	100	0.0	42.75	1
CGGTTTT	440	0.0	41.931816	1
AGGCGAT	250	0.0	40.499996	7
CGAATAT	90	0.0	40.0	14
CGTTTAG	45	1.9250365E-8	40.0	2
AGCGACT	80	0.0	39.375	19
>>END_MODULE