##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934586.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368103
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.073761963363516	33.0	31.0	34.0	30.0	34.0
2	32.27369513424232	33.0	31.0	34.0	30.0	34.0
3	32.16395411067011	33.0	31.0	34.0	30.0	34.0
4	35.90172587563807	37.0	35.0	37.0	35.0	37.0
5	32.13835529729451	37.0	35.0	37.0	0.0	37.0
6	34.019089765636245	37.0	35.0	37.0	19.0	37.0
7	20.691483090330696	32.0	0.0	37.0	0.0	37.0
8	28.07147184347859	35.0	17.0	37.0	17.0	37.0
9	34.858629242358795	37.0	32.0	39.0	32.0	39.0
10	36.26793316001228	37.0	35.0	39.0	32.0	39.0
11	36.754848507075465	39.0	37.0	39.0	33.0	39.0
12	37.10275928204877	39.0	37.0	39.0	34.0	39.0
13	36.85704816314999	39.0	37.0	39.0	33.0	39.0
14	38.27354571954046	40.0	38.0	41.0	34.0	41.0
15	38.46563054362502	40.0	38.0	41.0	34.0	41.0
16	38.54430689236437	40.0	38.0	41.0	34.0	41.0
17	38.53752889816165	40.0	38.0	41.0	34.0	41.0
18	38.457219854225585	40.0	38.0	41.0	34.0	41.0
19	38.39980657587686	40.0	37.0	41.0	34.0	41.0
20	38.370885322858	40.0	37.0	41.0	34.0	41.0
21	38.26958758825655	40.0	37.0	41.0	34.0	41.0
22	38.21756410569868	40.0	37.0	41.0	34.0	41.0
23	38.18351113682855	40.0	37.0	41.0	34.0	41.0
24	38.08424544217244	40.0	37.0	41.0	34.0	41.0
25	37.97497167912242	40.0	36.0	41.0	33.0	41.0
26	37.81427480895293	40.0	36.0	41.0	33.0	41.0
27	37.811080050963994	40.0	36.0	41.0	33.0	41.0
28	37.72838852169094	40.0	36.0	41.0	33.0	41.0
29	37.72503348247637	40.0	36.0	41.0	33.0	41.0
30	37.57088640951038	40.0	36.0	41.0	33.0	41.0
31	37.487817268536254	40.0	36.0	41.0	33.0	41.0
32	37.397467556634965	40.0	36.0	41.0	33.0	41.0
33	37.28105992072871	40.0	36.0	41.0	32.0	41.0
34	37.15320983529067	40.0	36.0	41.0	32.0	41.0
35	37.04466412933336	40.0	35.0	41.0	31.0	41.0
36	36.91736008671486	40.0	35.0	41.0	31.0	41.0
37	36.81447583964271	40.0	35.0	41.0	31.0	41.0
38	36.73928764503413	40.0	35.0	41.0	31.0	41.0
39	36.691648804818215	39.0	35.0	41.0	30.0	41.0
40	36.589457298636525	39.0	35.0	41.0	30.0	41.0
41	36.527773476445454	39.0	35.0	41.0	30.0	41.0
42	36.420175874687246	39.0	35.0	41.0	30.0	41.0
43	36.31776975466106	39.0	35.0	41.0	30.0	41.0
44	36.18390776494623	39.0	35.0	40.0	29.0	41.0
45	36.113571473201795	39.0	35.0	40.0	29.0	41.0
46	35.99752514921096	39.0	35.0	40.0	29.0	41.0
47	35.967166798423264	39.0	35.0	40.0	29.0	41.0
48	35.86310625015281	39.0	35.0	40.0	28.0	41.0
49	35.755921576297936	39.0	35.0	40.0	28.0	41.0
50	35.630089947650525	38.0	35.0	40.0	27.0	41.0
51	33.88477953181582	37.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	10.0
9	22.0
10	31.0
11	13.0
12	10.0
13	8.0
14	13.0
15	22.0
16	34.0
17	69.0
18	88.0
19	205.0
20	327.0
21	591.0
22	862.0
23	1426.0
24	2170.0
25	2926.0
26	3687.0
27	4021.0
28	4573.0
29	5243.0
30	6582.0
31	8391.0
32	11341.0
33	17108.0
34	24308.0
35	31822.0
36	39713.0
37	71063.0
38	78468.0
39	52949.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91300532731328	20.444006161318978	25.948172114870022	12.694816396497718
2	32.43874676381339	25.32198868251549	25.481726581962118	16.757537971709006
3	32.08449808885013	24.304338731279017	27.124201650081638	16.486961529789216
4	29.149450018065597	26.43336240128442	25.796312445158016	18.620875135491968
5	22.844692925621363	37.99751699931812	22.300551747744517	16.857238327315997
6	25.76262622146519	37.05049945259887	23.86804780183807	13.318826524097874
7	48.92136168409385	43.8132805220278	4.747584235933964	2.5177735579443796
8	85.23592581424221	5.463144826312201	6.608204768773958	2.6927245906716326
9	78.52068578631524	9.0844138732909	8.36179004246094	4.033110297932915
10	39.15616009649473	31.630005732091288	16.427738975232476	12.786095196181504
11	32.78185725191047	26.82455725707207	23.643925749043067	16.749659741974394
12	30.625395609381066	24.274727454000647	27.331752254124524	17.768124682493756
13	24.487167993740883	27.611565241250414	28.09023561340168	19.811031151607022
14	19.867537075220795	30.45370453378538	28.467847314474483	21.21091107651934
15	20.356530644955352	27.92071784256037	32.86824611589691	18.854505396587367
16	22.802041819816736	24.83951502704406	32.793267101870946	19.56517605126826
17	22.168251820821887	26.699592233695462	28.334732398268965	22.79742354721369
18	23.142435676970848	26.784622782210416	29.25621361412431	20.81672792669443
19	25.21957169596553	27.74359350507874	27.299424345903184	19.737410453052544
20	27.013906433797064	27.086712143068652	27.41026288837635	18.489118534757935
21	24.94763693857426	27.59390714012111	27.58141063778345	19.877045283521188
22	23.463269791335577	26.950608932825865	27.80390271201267	21.782218563825886
23	22.40378372357737	28.287191356766993	28.40672311825766	20.90230180139798
24	21.99221413571745	27.080463891899818	31.05679660312467	19.87052536925806
25	22.155483655389933	28.094853886004735	28.343697280380763	21.405965178224573
26	21.175051548072144	30.280926805812502	28.024493144581815	20.51952850153354
27	21.071819572239292	29.862022314406566	28.65013325074775	20.416024862606392
28	20.242975471539214	29.236653871334926	30.678098249674683	19.842272407451176
29	21.000643841533485	28.008465022018292	30.233657427404832	20.757233709043394
30	20.535013297908467	28.505065158393165	30.068757929166566	20.891163614531802
31	23.81235686750719	28.51185673575059	27.199452327201897	20.476334069540318
32	23.284243812193868	28.284474725824023	27.70882062900873	20.72246083297338
33	22.858547743430506	30.019586909098813	26.670795945699982	20.4510694017707
34	20.214179183543738	29.593347514146856	28.536578077331615	21.65589522497779
35	21.988682515491586	28.32984246257162	28.334189072080367	21.347285949856428
36	22.389385579579628	29.10788556463816	28.130441751357637	20.372287104424576
37	21.94983469300712	30.915803457184538	27.64199150781167	19.492370341996672
38	20.830039418314982	29.99649554608357	27.887031618867546	21.286433416733903
39	21.26252706443577	29.038068149403834	28.717505698133404	20.981899088026992
40	21.90066367293937	28.982377215072958	28.142666590601	20.97429252138668
41	19.716492394791675	29.031548235140708	29.04757635770423	22.204383012363387
42	21.002545483193565	29.19155779768163	26.661016074305287	23.144880644819523
43	22.241600856282073	27.887303281961845	28.213027332023916	21.658068529732166
44	21.368204008117292	29.453169357489617	27.24780835798676	21.930818276406335
45	20.711050983012907	30.602304246365826	26.582233776959168	22.1044109936621
46	20.625205445215062	31.662876966501223	26.785709434587602	20.926208153696113
47	21.944944757309777	28.80416622521414	28.145111558449678	21.105777459026413
48	21.71484611644024	27.899528121205208	29.074199340945334	21.311426421409223
49	20.619228857140527	27.687630907653567	28.799819615705385	22.89332061950052
50	19.174796184763505	29.358087274485673	29.75281375049918	21.71430279025164
51	20.189186178868415	28.999491990013667	27.79385117752368	23.017470653594238
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	302.0
1	733.0
2	1164.0
3	2001.0
4	2838.0
5	2088.0
6	1338.0
7	1220.5
8	1103.0
9	1081.0
10	1059.0
11	1071.0
12	1083.0
13	1085.5
14	1088.0
15	1056.0
16	1024.0
17	1081.0
18	1138.0
19	1242.0
20	1346.0
21	1639.0
22	1932.0
23	2250.5
24	2569.0
25	3035.5
26	4053.5
27	4605.0
28	5654.5
29	6704.0
30	8207.5
31	9711.0
32	11154.0
33	12597.0
34	13813.0
35	15029.0
36	15684.0
37	16339.0
38	18097.0
39	19855.0
40	21566.0
41	23277.0
42	24387.5
43	25498.0
44	26174.0
45	26850.0
46	27053.5
47	27257.0
48	26861.0
49	26465.0
50	24803.5
51	23142.0
52	21542.0
53	19942.0
54	19184.5
55	18427.0
56	17439.5
57	16452.0
58	15428.5
59	14405.0
60	13393.5
61	12382.0
62	10963.0
63	9544.0
64	8012.5
65	6481.0
66	5306.0
67	4131.0
68	3574.5
69	3018.0
70	2403.5
71	1789.0
72	1408.0
73	1027.0
74	867.0
75	581.5
76	456.0
77	343.5
78	231.0
79	185.0
80	139.0
81	103.5
82	68.0
83	52.0
84	36.0
85	31.5
86	27.0
87	21.5
88	16.0
89	10.5
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	368103.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.631710939551176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.07450793627558	22.213876653287272
2	8.044103207038418	5.249857012399885
3	4.255455469881754	4.1658837842794
4	3.034955308308434	3.96143137340709
5	2.3203644233082117	3.7858730567905967
6	1.805807964374302	3.535596210348093
7	1.4904416624885402	3.40449630518131
8	1.3441168515827875	3.5088666055863307
9	1.1292845155792341	3.3165437292803386
>10	8.4332558840701	44.29332139436504
>50	0.047645509805956235	1.0171367620628975
>100	0.019225381149771816	1.3242689675021098
>500	8.358861369466006E-4	0.22284814550962384
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	817	0.22194874804062995	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	458	0.12442169718801532	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	408	0.11083854247316646	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01711477494070953	0.0
2	0.0	0.0	0.0	0.08801884255222044	0.0
3	0.0	0.0	0.0	0.12007508767926368	0.0
4	0.0	0.0	0.0	0.1581079208808404	0.0
5	0.0	0.0	0.0	0.26948978954260083	0.0
6	0.0	0.0	0.0	0.35234703330317874	0.0
7	0.0	0.0	0.0	0.44036587585539916	0.0
8	0.0	0.0	0.0	0.6310733680518767	0.0
9	0.0	0.0	0.0	0.7049657297006544	0.0
10	0.0	0.0	0.0	0.9119730075549507	0.0
11	2.7166309429697664E-4	0.0	0.0	1.200750876792637	0.0
12	2.7166309429697664E-4	0.0	0.0	1.4112897748727937	0.0
13	2.7166309429697664E-4	0.0	0.0	1.513978424517051	0.0
14	2.7166309429697664E-4	0.0	0.0	1.560161150547537	0.0
15	2.7166309429697664E-4	0.0	0.0	1.6359551538563935	0.0
16	2.7166309429697664E-4	0.0	0.0	1.8326392341274045	0.0
17	2.7166309429697664E-4	0.0	0.0	2.0429064691132646	0.0
18	2.7166309429697664E-4	0.0	0.0	2.2765367302086643	0.0
19	2.7166309429697664E-4	0.0	0.0	2.4259514320720017	0.0
20	2.7166309429697664E-4	0.0	0.0	2.5875909731787026	0.0
21	2.7166309429697664E-4	0.0	0.0	2.77829846537518	0.0
22	2.7166309429697664E-4	0.0	0.0	3.0067671276789376	0.0
23	2.7166309429697664E-4	0.0	0.0	3.2268142340594888	0.0
24	2.7166309429697664E-4	0.0	0.0	3.411001811992839	0.0
25	2.7166309429697664E-4	0.0	0.0	3.5805195828341523	0.0
26	2.7166309429697664E-4	0.0	0.0	3.7416157977522597	0.0
27	2.7166309429697664E-4	0.0	0.0	3.901625360293179	0.0
28	2.7166309429697664E-4	0.0	0.0	4.052126714533704	0.0
29	2.7166309429697664E-4	0.0	0.0	4.234684313901272	0.0
30	2.7166309429697664E-4	0.0	0.0	4.441148265566975	0.0
31	2.7166309429697664E-4	0.0	0.0	4.633214073234937	0.0
32	2.7166309429697664E-4	0.0	0.0	4.811153399999457	0.0
33	2.7166309429697664E-4	0.0	0.0	4.992081020801243	0.0
34	2.7166309429697664E-4	0.0	0.0	5.172193652320138	0.0
35	2.7166309429697664E-4	0.0	0.0	5.3743109944770895	0.0
36	2.7166309429697664E-4	0.0	0.0	5.57018008546521	0.0
37	2.7166309429697664E-4	0.0	0.0	5.776915700225208	0.0
38	2.7166309429697664E-4	0.0	0.0	5.997506132794354	0.0
39	2.7166309429697664E-4	0.0	0.0	6.221084859400766	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	20	7.027031E-4	45.000004	2
TCCGAAC	20	7.027031E-4	45.000004	36
GAGTACG	20	7.027031E-4	45.000004	1
GTCTAGT	20	7.027031E-4	45.000004	19
TACGGCT	20	7.027031E-4	45.000004	15
CGACACC	20	7.027031E-4	45.000004	35
ACGGGTC	20	7.027031E-4	45.000004	5
ACGATTT	20	7.027031E-4	45.000004	11
AGACGCA	20	7.027031E-4	45.000004	19
ACGGCTA	20	7.027031E-4	45.000004	23
GGTATCG	20	7.027031E-4	45.000004	8
AACGAGT	20	7.027031E-4	45.000004	36
CGCAGTA	20	7.027031E-4	45.000004	37
AGCGAAC	20	7.027031E-4	45.000004	41
GCGCTAC	20	7.027031E-4	45.000004	20
GCATGCG	20	7.027031E-4	45.000004	1
CCGCCAC	20	7.027031E-4	45.000004	40
CCCGATT	20	7.027031E-4	45.000004	30
CTATGCG	25	3.885622E-5	45.0	18
TCCAGGT	25	3.885622E-5	45.0	11
>>END_MODULE