##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934585.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	496901
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.866405984290633	31.0	31.0	34.0	30.0	34.0
2	32.03732332999934	33.0	31.0	34.0	30.0	34.0
3	31.69238540473857	33.0	31.0	34.0	30.0	34.0
4	35.59929241438435	37.0	35.0	37.0	33.0	37.0
5	32.024137604875015	37.0	35.0	37.0	10.0	37.0
6	33.87964202124769	37.0	35.0	37.0	19.0	37.0
7	20.8303444750564	32.0	0.0	37.0	0.0	37.0
8	28.05102022334429	35.0	17.0	37.0	17.0	37.0
9	34.71851737066337	37.0	32.0	39.0	30.0	39.0
10	36.2349099720065	37.0	35.0	39.0	32.0	39.0
11	36.66226069176758	38.0	35.0	39.0	33.0	39.0
12	36.923540101549406	39.0	37.0	39.0	33.0	39.0
13	36.672294883689105	39.0	35.0	39.0	33.0	39.0
14	37.96948285473364	40.0	37.0	41.0	33.0	41.0
15	38.13152519314713	40.0	37.0	41.0	33.0	41.0
16	38.217576539391146	40.0	37.0	41.0	33.0	41.0
17	38.16739752989026	40.0	37.0	41.0	33.0	41.0
18	38.01087339329162	40.0	37.0	41.0	33.0	41.0
19	37.9049347858024	40.0	37.0	41.0	33.0	41.0
20	37.873588501532495	40.0	36.0	41.0	33.0	41.0
21	37.80183980309961	40.0	36.0	41.0	33.0	41.0
22	37.80601166027036	39.0	36.0	41.0	33.0	41.0
23	37.740952423118486	39.0	36.0	41.0	33.0	41.0
24	37.60513462440204	39.0	35.0	41.0	33.0	41.0
25	37.463341792429475	39.0	35.0	41.0	33.0	41.0
26	37.311448356916166	39.0	35.0	41.0	33.0	41.0
27	37.28238220490601	39.0	35.0	41.0	33.0	41.0
28	37.183398705174675	39.0	35.0	41.0	33.0	41.0
29	37.130859064481655	39.0	35.0	41.0	32.0	41.0
30	36.94003433279466	39.0	35.0	41.0	32.0	41.0
31	36.76661548276216	39.0	35.0	41.0	31.0	41.0
32	36.58339186276542	39.0	35.0	40.0	31.0	41.0
33	36.36011801143487	39.0	35.0	40.0	30.0	41.0
34	36.07648807307693	39.0	35.0	40.0	29.0	41.0
35	35.818317934558394	39.0	35.0	40.0	27.0	41.0
36	35.6446696625686	39.0	35.0	40.0	26.0	41.0
37	35.51765240963492	39.0	35.0	40.0	25.0	41.0
38	35.33233782986953	38.0	34.0	40.0	25.0	41.0
39	35.18757659976534	38.0	34.0	40.0	24.0	41.0
40	35.03046884590693	38.0	34.0	40.0	23.0	41.0
41	34.88689900000201	38.0	34.0	40.0	23.0	41.0
42	34.75822749400786	38.0	34.0	40.0	23.0	41.0
43	34.65740459367158	38.0	34.0	40.0	22.0	41.0
44	34.51437207814031	38.0	33.0	40.0	22.0	41.0
45	34.41579509801751	38.0	33.0	40.0	22.0	41.0
46	34.25596648024455	38.0	33.0	40.0	22.0	41.0
47	34.155212004000795	37.0	33.0	40.0	21.0	41.0
48	34.03104240080016	37.0	33.0	40.0	20.0	41.0
49	33.92155177791955	37.0	33.0	40.0	20.0	41.0
50	33.78944095503933	37.0	33.0	40.0	20.0	41.0
51	31.934129736104374	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	16.0
9	20.0
10	21.0
11	13.0
12	6.0
13	16.0
14	17.0
15	25.0
16	70.0
17	126.0
18	269.0
19	466.0
20	760.0
21	1331.0
22	2128.0
23	3343.0
24	5056.0
25	6987.0
26	8711.0
27	9472.0
28	9712.0
29	10724.0
30	12118.0
31	14641.0
32	19093.0
33	27074.0
34	35429.0
35	43538.0
36	55116.0
37	88315.0
38	87480.0
39	54799.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39013606332046	18.602095789704588	23.700093177514233	16.307674969460717
2	35.49982793353203	24.115266421279088	23.837142609896137	16.547763035292746
3	30.451136141806916	24.18167804049499	29.948822803737567	15.418363013960526
4	26.280285207717434	24.059520910603922	31.712151917585192	17.94804196409345
5	23.26801515794897	35.10739563816535	25.336233978196866	16.288355225688818
6	25.349315054709088	33.25410896737982	28.108818456795216	13.287757521115875
7	46.95301478564141	42.195930376473385	8.442929275650481	2.408125562234731
8	80.49369995230438	5.143680531936945	11.546968108335463	2.815651407423209
9	74.41743928871144	7.061567595959758	12.842195930376473	5.678797184952335
10	38.46500610785649	30.07178492295246	19.43988842847972	12.023320540711328
11	32.23378499942645	23.7822020885448	27.40666651908529	16.57734639294346
12	28.922662663186426	22.22957893020944	30.409075449636845	18.438682956967284
13	25.342271398125582	22.57471810280116	33.102770974499954	18.980239524573307
14	21.017063761191867	24.63549077180364	33.593814462035695	20.7536310049688
15	20.555804878637797	23.71518672733603	36.24544929472873	19.483559099297445
16	25.327379095634743	24.307457622343286	31.497823510115698	18.867339771906273
17	25.247483905244707	23.268216405279926	31.16697289802194	20.31732679145343
18	25.80997019527029	22.701101426642328	32.00858923608526	19.48033914200213
19	24.489385209528656	24.79165870062648	29.857657762813922	20.861298327030937
20	25.610936584953542	26.121501063592145	30.676533152479063	17.59102919897525
21	25.62220643548715	25.181676028021677	31.96914475921763	17.22697277727354
22	24.810777197067424	22.322152702449785	31.775746074167692	21.0913240263151
23	22.59202537326349	25.093328449731434	32.71355863642859	19.601087540576493
24	22.943403213114884	23.694257004916473	32.6324559620528	20.72988381991584
25	23.026920855462155	25.949233348292715	31.14262197097611	19.881223825269018
26	22.706535104578176	24.822650789593904	30.470254638247862	22.00055946758006
27	22.0154517700709	24.454971915934966	31.837931499433488	21.691644814560647
28	20.261782528109222	25.356962453285465	32.63507217735525	21.746182841250068
29	22.69788146934701	24.554790592089773	30.997723892686874	21.749604045876342
30	22.92408346934299	24.1764456098901	32.56725182682265	20.332219093944268
31	24.347103346541864	23.38896480385429	31.687800990539362	20.57613085906448
32	25.637903727301815	24.19314913835955	30.581946906929147	19.587000227409483
33	24.32194743017221	23.796490649042767	30.47266960621935	21.408892314565676
34	22.052883773628952	24.83452438212038	32.22794882682869	20.88464301742198
35	22.587195437320513	26.02570733405648	31.200581202291804	20.1865160263312
36	23.108224777168893	27.204211704142274	29.735903127584773	19.951660391104063
37	23.215690851900074	26.931119076033255	28.892475563542842	20.960714508523832
38	23.28854238570661	27.12471900841415	28.965125850018413	20.621612755860824
39	23.537082839438842	25.43243020239444	28.427594229031538	22.602892729135178
40	24.83311565080368	24.82365702624869	29.442887013710983	20.900340309236647
41	21.81561317043033	26.17201414366242	28.81338536247663	23.19898732343062
42	22.57109565084393	24.964328910587824	30.04280530729461	22.421770131273632
43	22.611143869704428	25.18972592125997	29.112640143610097	23.086490065425508
44	22.977212764715706	24.918444519129565	29.586577608014476	22.517765108140253
45	22.100176896403912	24.82808446752975	29.548743109794508	23.522995526271835
46	22.821044835892863	26.04381959384264	28.85604979663957	22.279085773624928
47	21.154314440904727	25.884029213062558	31.2392206898356	21.72243565619711
48	22.19415939996096	26.359174161452685	29.92326439270599	21.523402045880367
49	22.73209351560975	25.246075173928006	30.681966830414915	21.339864480047332
50	21.08045667044341	26.304032392770395	30.201388204088943	22.41412273269726
51	21.85747261526944	25.46362353869282	28.882413196994978	23.796490649042767
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	425.0
1	866.5
2	1308.0
3	6903.5
4	12499.0
5	8286.5
6	4074.0
7	3541.5
8	3009.0
9	2889.5
10	2770.0
11	2709.5
12	2649.0
13	2597.5
14	2546.0
15	2431.5
16	2317.0
17	2201.5
18	2086.0
19	2169.0
20	2252.0
21	2337.5
22	2423.0
23	2560.0
24	2697.0
25	3175.0
26	4449.5
27	5246.0
28	6105.0
29	6964.0
30	7888.0
31	8812.0
32	10237.0
33	11662.0
34	13079.5
35	14497.0
36	15594.0
37	16691.0
38	18293.5
39	19896.0
40	20925.0
41	21954.0
42	23381.5
43	24809.0
44	27326.0
45	29843.0
46	33881.5
47	37920.0
48	36088.0
49	34256.0
50	33934.0
51	33612.0
52	32239.5
53	30867.0
54	29794.0
55	28721.0
56	27734.5
57	26748.0
58	25726.5
59	24705.0
60	22944.5
61	21184.0
62	19030.0
63	16876.0
64	14824.0
65	12772.0
66	10806.0
67	8840.0
68	7321.0
69	5802.0
70	4767.0
71	3732.0
72	3055.5
73	2379.0
74	1991.0
75	1276.0
76	949.0
77	683.5
78	418.0
79	308.0
80	198.0
81	152.0
82	106.0
83	83.0
84	60.0
85	44.5
86	29.0
87	24.0
88	19.0
89	13.5
90	8.0
91	8.5
92	9.0
93	7.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	496901.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.487621355477685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.215818810916	29.833031228030265
2	8.939308090670062	7.596198746726955
3	4.69318675027553	5.982070247887545
4	3.281114822753775	5.5762705685203064
5	2.5297914216150144	5.374241001495717
6	2.092700832595961	5.334832835137806
7	1.7615506082646515	5.239086666972193
8	1.4365859588034162	4.882969620978832
9	1.157997693717918	4.4280510787082985
>10	3.8535017851659386	21.431374278548606
>50	0.021528206124141684	0.6348330518622424
>100	0.01281440840719505	0.9956969856290571
>500	0.0020503053451512085	0.674251502079519
>1k	0.0020503053451512085	2.017092187422644
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4432	0.8919281708026348	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2483	0.4996971227669093	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG	1271	0.2557853576466942	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	1076	0.21654212811002593	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	933	0.18776375978313586	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG	794	0.1597903807800749	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	768	0.1545579501751858	No Hit
GAATCTATCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	601	0.12094964590532119	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.012473309572732E-4	0.0	0.0	0.019118496440940953	0.0
2	2.012473309572732E-4	0.0	0.0	0.11591846263138936	0.0
3	2.012473309572732E-4	0.0	0.0	0.15636917615380125	0.0
4	2.012473309572732E-4	0.0	0.0	0.26625021885647243	0.0
5	2.012473309572732E-4	0.0	0.0	0.716038003545978	0.0
6	2.012473309572732E-4	0.0	0.0	0.8695897170663774	0.0
7	2.012473309572732E-4	0.0	0.0	1.0645983807639752	0.0
8	2.012473309572732E-4	0.0	0.0	1.3712993131428595	0.0
9	2.012473309572732E-4	0.0	0.0	1.523442295346558	0.0
10	2.012473309572732E-4	0.0	0.0	2.3401039643711727	0.0
11	2.012473309572732E-4	0.0	0.0	2.604945451910944	0.0
12	2.012473309572732E-4	0.0	0.0	3.12778601773794	0.0
13	2.012473309572732E-4	0.0	0.0	3.2352520924691235	0.0
14	2.012473309572732E-4	0.0	0.0	3.3072986369518276	0.0
15	2.012473309572732E-4	0.0	0.0	3.4075198077685496	0.0
16	2.012473309572732E-4	0.0	0.0	3.5264569803642978	0.0
17	2.012473309572732E-4	0.0	0.0	3.6395579803622855	0.0
18	4.024946619145464E-4	0.0	0.0	3.777009907406103	0.0
19	4.024946619145464E-4	0.0	0.0	3.8798472935252697	0.0
20	4.024946619145464E-4	0.0	0.0	3.987112120925496	0.0
21	4.024946619145464E-4	0.0	0.0	4.11671540206198	0.0
22	4.024946619145464E-4	0.0	0.0	4.255173565760584	0.0
23	4.024946619145464E-4	0.0	0.0	4.396046697430675	0.0
24	4.024946619145464E-4	0.0	0.0	4.509550192090577	0.0
25	4.024946619145464E-4	0.0	0.0	4.6141988041883595	0.0
26	4.024946619145464E-4	0.0	0.0	4.716834942976568	0.0
27	4.024946619145464E-4	0.0	0.0	4.8532806333656	0.0
28	4.024946619145464E-4	0.0	0.0	4.975236515925707	0.0
29	4.024946619145464E-4	0.0	0.0	5.115505905602927	0.0
30	4.024946619145464E-4	0.0	0.0	5.311520805955311	0.0
31	4.024946619145464E-4	0.0	0.0	5.452192690294445	0.0
32	4.024946619145464E-4	0.0	0.0	5.600511973209955	0.0
33	4.024946619145464E-4	0.0	0.0	5.744202567513448	0.0
34	4.024946619145464E-4	0.0	0.0	5.900772990998207	0.0
35	4.024946619145464E-4	0.0	0.0	6.077468147578693	0.0
36	4.024946619145464E-4	0.0	0.0	6.213712590636766	0.0
37	4.024946619145464E-4	0.0	0.0	6.374509208071628	0.0
38	4.024946619145464E-4	0.0	0.0	6.5781715070003886	0.0
39	4.024946619145464E-4	0.0	0.0	6.921499453613497	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	20	7.029417E-4	45.0	1
GCGAATA	20	7.029417E-4	45.0	1
TCGATCA	50	2.1827873E-11	45.0	17
GCGCAAA	20	7.029417E-4	45.0	1
TCCGTCC	20	7.029417E-4	45.0	43
CGGTAAT	20	7.029417E-4	45.0	40
CGACGGT	30	2.1630058E-6	44.999996	28
CGGTCTA	30	2.1630058E-6	44.999996	31
CGTTTTA	845	0.0	44.467457	1
CGTTATT	405	0.0	42.77778	1
CGTTTTT	1745	0.0	41.647564	1
TATGCGG	60	3.6379788E-12	41.249996	2
AGGCGAT	290	0.0	40.344826	7
CGTAGGA	40	3.452933E-7	39.375	4
GTTTTAT	1060	0.0	38.84434	2
TCACGAC	35	6.2411327E-6	38.571426	25
CTCACGT	35	6.2411327E-6	38.571426	45
TACGGCT	140	0.0	38.571426	7
ACGACCA	170	0.0	38.38235	28
AAACACG	180	0.0	37.500004	40
>>END_MODULE