##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934583.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	477823
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61065080584233	31.0	31.0	34.0	30.0	34.0
2	31.79355535417927	31.0	31.0	34.0	30.0	34.0
3	31.254351506729478	31.0	31.0	34.0	28.0	34.0
4	35.2739194220454	37.0	35.0	37.0	33.0	37.0
5	31.782733355238236	35.0	35.0	37.0	0.0	37.0
6	33.7350127557694	37.0	35.0	37.0	19.0	37.0
7	21.140951775029666	32.0	0.0	37.0	0.0	37.0
8	28.285844339849692	35.0	17.0	37.0	17.0	37.0
9	34.805821402485854	37.0	32.0	39.0	31.0	39.0
10	36.09540562090984	37.0	35.0	39.0	32.0	39.0
11	36.53858855685055	38.0	35.0	39.0	32.0	39.0
12	36.91100888822849	39.0	37.0	39.0	33.0	39.0
13	36.680710221148836	39.0	35.0	39.0	33.0	39.0
14	38.02612892221597	40.0	37.0	41.0	33.0	41.0
15	38.19952158016672	40.0	37.0	41.0	33.0	41.0
16	38.27721980733452	40.0	37.0	41.0	34.0	41.0
17	38.25937428713143	40.0	37.0	41.0	34.0	41.0
18	38.09983822461455	40.0	37.0	41.0	33.0	41.0
19	37.961309941128825	40.0	37.0	41.0	34.0	41.0
20	37.82409385902311	39.0	36.0	41.0	33.0	41.0
21	37.70060043154055	39.0	36.0	41.0	33.0	41.0
22	37.66399901218652	39.0	35.0	41.0	33.0	41.0
23	37.573647564056145	39.0	35.0	41.0	33.0	41.0
24	37.392519824286396	39.0	35.0	41.0	33.0	41.0
25	37.22360581219405	39.0	35.0	41.0	33.0	41.0
26	37.10027562507456	39.0	35.0	40.0	32.0	41.0
27	37.06939808255358	39.0	35.0	40.0	32.0	41.0
28	36.96694382647968	39.0	35.0	40.0	32.0	41.0
29	36.950611418872676	39.0	35.0	40.0	32.0	41.0
30	36.74830847405838	39.0	35.0	40.0	31.0	41.0
31	36.551065980499054	39.0	35.0	40.0	31.0	41.0
32	36.35216387658191	39.0	35.0	40.0	30.0	41.0
33	35.982979052912896	38.0	35.0	40.0	29.0	41.0
34	35.61736668180477	38.0	35.0	40.0	26.0	41.0
35	35.29828827829552	38.0	34.0	40.0	25.0	41.0
36	34.994763751430966	38.0	34.0	40.0	23.0	41.0
37	34.850375557476305	38.0	34.0	40.0	23.0	41.0
38	34.667307768776304	38.0	34.0	40.0	21.0	41.0
39	34.573741741188684	38.0	34.0	40.0	21.0	41.0
40	34.47663883906802	38.0	34.0	40.0	20.0	41.0
41	34.35908694223593	38.0	33.0	40.0	20.0	41.0
42	34.21585608059888	38.0	33.0	40.0	18.0	41.0
43	34.10804209927107	38.0	33.0	40.0	18.0	41.0
44	33.961408303911696	38.0	33.0	40.0	18.0	41.0
45	33.84927473143821	38.0	33.0	40.0	18.0	41.0
46	33.69835483013585	37.0	33.0	40.0	18.0	41.0
47	33.64002779271822	37.0	33.0	40.0	17.0	41.0
48	33.496596019865095	37.0	33.0	40.0	15.0	41.0
49	33.3223955313997	37.0	33.0	40.0	15.0	41.0
50	33.10530886960234	37.0	32.0	40.0	14.0	41.0
51	31.184647871701447	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	12.0
9	20.0
10	22.0
11	18.0
12	10.0
13	13.0
14	11.0
15	27.0
16	49.0
17	93.0
18	201.0
19	392.0
20	720.0
21	1324.0
22	2309.0
23	3971.0
24	6359.0
25	8782.0
26	10706.0
27	10521.0
28	10578.0
29	10713.0
30	11958.0
31	14420.0
32	18668.0
33	25633.0
34	34403.0
35	43606.0
36	53204.0
37	81348.0
38	79147.0
39	48573.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.49275359285761	19.763175904048154	24.38120391860584	18.362866584488398
2	38.0724661642491	21.170182264143836	24.961335055030837	15.796016516576222
3	29.664331771388152	21.144440514583852	33.92699807250802	15.264229641519977
4	24.197244586384496	24.217544990509037	33.828216724603045	17.756993698503422
5	18.942997720913393	35.38883645199164	29.525577462784337	16.142588364310633
6	23.02358823246265	31.333569124968868	32.264457759463234	13.378384883105252
7	45.354869899523464	41.20647185254791	10.758167773422377	2.68049047450625
8	77.91043963978294	3.3836797307789706	15.427470004583288	3.2784106248548097
9	72.56138779422507	6.034242805390281	16.973230673282785	4.431138727101876
10	36.42311064975943	26.389269666801308	24.366972707466992	12.820646975972275
11	27.52190664744058	23.604347216437883	31.766574652120138	17.107171484001398
12	25.35918111936847	21.210783072392918	35.38967358205863	18.040362226179987
13	20.866094767309235	24.24265889251878	37.33495457522974	17.556291764942248
14	18.175977297032585	25.52974637051795	36.95782748005014	19.336448852399318
15	16.428468282188174	24.839951195317095	39.8842249117351	18.847355610759635
16	20.47515502602428	23.485684029441863	37.32302547177511	18.71613547275874
17	20.32363448389885	23.188084290626445	34.521360420071865	21.966920805402836
18	21.153021097770512	23.63971596176827	36.154810463288705	19.05245247717251
19	20.603026643757207	26.050441272186564	33.5808447898071	19.765687294249126
20	21.712433265037472	25.389945649330404	34.302450907553634	18.595170178078494
21	20.842655125433478	25.441010583416872	35.55709959545689	18.159234695692756
22	20.474108613440542	23.511844344035342	34.29596314953445	21.718083892989664
23	18.941742025812903	24.79202549898184	34.979479849232874	21.28675262597238
24	19.056638127507465	23.385856268953148	37.24893946084638	20.308566142693003
25	18.860331126798	25.175221787147123	34.96859715836199	20.99584992769289
26	18.612749909485313	26.256584551183177	33.88367659154122	21.246988947790292
27	18.26220169393269	25.964007592769704	34.33614539275003	21.437645320547567
28	16.803083987166794	25.01993415972023	36.197504096705266	21.979477756407707
29	17.87000625754725	23.7431015250417	35.41269465890089	22.97419755851016
30	18.42188425421129	24.711242447517176	34.91460226904105	21.952271029230488
31	19.558079037635277	24.97284559345197	34.190275478576794	21.27879989033596
32	20.292242106386674	25.110134924438547	33.001132218415606	21.596490750759173
33	19.586960024946475	25.597972470977748	32.964298495467986	21.85076900860779
34	18.002063525615135	25.027468330323156	34.83088926234191	22.1395788817198
35	18.548500176843728	25.56762650604931	33.16290760386168	22.72096571324528
36	20.45987740230169	26.848435508546054	31.7891771639289	20.902509925223356
37	19.091588307804354	27.717167235566308	32.429163100143775	20.76208135648556
38	19.285593200829595	27.68347275036991	31.418956391801984	21.611977656998512
39	19.251480150599697	27.62696647084799	31.907840350924925	21.213713027627385
40	21.292821818958068	25.471775113378804	32.31803408375068	20.91736898391245
41	19.10833090914418	25.810812790510298	31.56168706822401	23.519169232121516
42	19.819891466086816	26.119085937679852	30.87544969580786	23.185572900425473
43	19.396722217222695	26.031815128196005	32.0118537617486	22.559608892832703
44	19.078822074282737	26.265792981920082	31.057734767895223	23.597650175901954
45	18.698346458835175	25.967146830520925	30.992647905186647	24.34185880545725
46	19.664394556143176	26.590390165395974	30.646494622485733	23.09872065597512
47	17.877958993183668	26.702984159406306	32.14098107458201	23.278075772828014
48	19.033407768148454	25.03332824079209	32.26968982238193	23.663574168677524
49	19.21150718990086	25.07895182944312	32.88393400903682	22.825606971619198
50	18.358680934153444	25.750120860653418	32.303384307578334	23.587813897614808
51	17.975693928504906	25.560720182996633	30.715976418046015	25.74760947045245
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	400.0
1	802.0
2	1204.0
3	8029.5
4	14855.0
5	10454.0
6	6053.0
7	5292.5
8	4532.0
9	4331.0
10	4130.0
11	4021.5
12	3913.0
13	3914.0
14	3915.0
15	3853.0
16	3791.0
17	3601.0
18	3411.0
19	3340.5
20	3270.0
21	3338.5
22	3407.0
23	3489.5
24	3572.0
25	3974.5
26	5129.5
27	5882.0
28	6649.5
29	7417.0
30	8316.5
31	9216.0
32	10439.0
33	11662.0
34	13073.5
35	14485.0
36	16013.5
37	17542.0
38	19427.0
39	21312.0
40	23246.5
41	25181.0
42	27209.5
43	29238.0
44	31512.5
45	33787.0
46	36460.5
47	39134.0
48	39391.0
49	39648.0
50	37626.0
51	35604.0
52	31716.0
53	27828.0
54	24927.5
55	22027.0
56	19835.0
57	17643.0
58	16360.5
59	15078.0
60	13901.0
61	12724.0
62	11461.0
63	10198.0
64	8897.5
65	7597.0
66	6349.5
67	5102.0
68	4188.0
69	3274.0
70	2739.0
71	2204.0
72	1839.5
73	1475.0
74	1121.0
75	602.0
76	437.0
77	339.5
78	242.0
79	186.5
80	131.0
81	104.5
82	78.0
83	63.5
84	49.0
85	31.0
86	13.0
87	9.5
88	6.0
89	5.0
90	4.0
91	2.5
92	1.0
93	2.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	477823.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.72175446307235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.89215674572657	38.60315642207925
2	6.970065927721804	6.65247549588333
3	2.6263802056613166	3.760064139037284
4	1.5489296663630576	2.956705648749857
5	1.0912197507256123	2.6037460504691343
6	0.8057946457678643	2.307236053979543
7	0.7031572700699947	2.348912901384318
8	0.599584924971542	2.289059565540127
9	0.5636807736043917	2.420985192615311
>10	4.162472039272837	32.01434928238594
>50	0.024063526658035902	0.7457674001919686
>100	0.010643482944855636	0.9599795346827047
>500	4.627601280372015E-4	0.14553174910418737
>1k	9.25520256074403E-4	0.926853946874468
>5k	4.627601280372015E-4	1.265176617022594
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5729	1.1989795384483375	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3027	0.6334981781956917	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1170	0.2448605445949651	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	659	0.1379171785368222	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.092825167478334E-4	0.0	0.0	0.07931807384742887	0.0
2	2.092825167478334E-4	0.0	0.0	0.3495018029688818	0.0
3	2.092825167478334E-4	0.0	0.0	0.5026966052282958	0.0
4	2.092825167478334E-4	0.0	0.0	0.6552635599374664	0.0
5	2.092825167478334E-4	0.0	0.0	1.09538469265816	0.0
6	2.092825167478334E-4	0.0	0.0	1.553922686852663	0.0
7	2.092825167478334E-4	0.0	0.0	2.004926510444244	0.0
8	2.092825167478334E-4	0.0	0.0	2.9150961757805716	0.0
9	2.092825167478334E-4	0.0	0.0	3.233624166270774	0.0
10	2.092825167478334E-4	0.0	0.0	3.9784606433763128	0.0
11	2.092825167478334E-4	0.0	0.0	4.983853853832905	0.0
12	2.092825167478334E-4	0.0	0.0	5.725132528153731	0.0
13	2.092825167478334E-4	0.0	0.0	6.074425048605865	0.0
14	2.092825167478334E-4	0.0	0.0	6.216527877477644	0.0
15	2.092825167478334E-4	0.0	0.0	6.4337631298618945	0.0
16	2.092825167478334E-4	0.0	0.0	6.913857223281425	0.0
17	2.092825167478334E-4	0.0	0.0	7.501941095342836	0.0
18	2.092825167478334E-4	0.0	0.0	8.154902547596077	0.0
19	2.092825167478334E-4	0.0	0.0	8.556097132201673	0.0
20	2.092825167478334E-4	0.0	0.0	8.947664721036869	0.0
21	2.092825167478334E-4	0.0	0.0	9.515029623940245	0.0
22	2.092825167478334E-4	0.0	0.0	10.077581028958422	0.0
23	2.092825167478334E-4	0.0	0.0	10.755028535671158	0.0
24	2.092825167478334E-4	0.0	0.0	11.273212047138793	0.0
25	2.092825167478334E-4	0.0	0.0	11.701194793888114	0.0
26	2.092825167478334E-4	0.0	0.0	12.101761530943467	0.0
27	2.092825167478334E-4	0.0	0.0	12.506723200850525	0.0
28	2.092825167478334E-4	0.0	0.0	12.929473884681148	0.0
29	2.092825167478334E-4	0.0	0.0	13.381733403373216	0.0
30	2.092825167478334E-4	0.0	0.0	13.886313551252242	0.0
31	2.092825167478334E-4	0.0	0.0	14.353432128633406	0.0
32	2.092825167478334E-4	0.0	0.0	14.79166971870337	0.0
33	2.092825167478334E-4	0.0	0.0	15.210653317232532	0.0
34	2.092825167478334E-4	0.0	0.0	15.613940727005607	0.0
35	2.092825167478334E-4	0.0	0.0	16.031040782884038	0.0
36	2.092825167478334E-4	0.0	0.0	16.433072497556626	0.0
37	2.092825167478334E-4	0.0	0.0	16.856241746420746	0.0
38	2.092825167478334E-4	0.0	0.0	17.284224493170065	0.0
39	2.092825167478334E-4	0.0	0.0	17.785037555747632	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAG	20	7.0291426E-4	45.000004	2
CTAACGG	30	2.1628293E-6	45.000004	2
ACGTGCA	20	7.0291426E-4	45.000004	1
GCGATAC	20	7.0291426E-4	45.000004	9
TTACGCG	20	7.0291426E-4	45.000004	1
CGTCCAC	20	7.0291426E-4	45.000004	41
CGGTTTA	155	0.0	45.0	1
CGCGCAA	25	3.8873717E-5	45.0	18
GCGCAAA	25	3.8873717E-5	45.0	1
TACGCGG	25	3.8873717E-5	45.0	2
AACGACG	25	3.8873717E-5	45.0	13
AACGAAG	25	3.8873717E-5	45.0	2
AATAGGC	55	1.8189894E-12	45.0	4
CGCAATC	25	3.8873717E-5	45.0	20
ACGCGCA	25	3.8873717E-5	45.0	17
CGTTTTA	1165	0.0	43.648067	1
CGTTTTT	2550	0.0	42.97059	1
CGTTATT	595	0.0	42.73109	1
CATACGA	90	0.0	42.5	18
CGGTTTT	310	0.0	42.09677	1
>>END_MODULE