FastQCFastQC Report
Sat 14 Jan 2017
SRR2934582.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934582.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758460
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187382.4705323945890356No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108991.4369907444031327No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19420.2560451441077974No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8560.11286026949344724No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG207.031768E-445.02
AGTGCGA253.8895458E-545.01
ACTAGCG253.8895458E-545.01
TTAACGG253.8895458E-545.02
GCGCTAA253.8895458E-545.01
ACGAGAC207.031768E-445.04
ATCGCTC207.031768E-445.019
CCGCATT207.031768E-445.030
CGTTTTA44950.044.449391
CGTTATT19550.044.0792851
CGTTTTT81550.043.813611
CGGTTTA2750.042.5454561
GTTTTAT47950.041.9968722
CGAATAT650.041.5384614
CGGTTTT8150.040.3067471
CGCTAAG403.4561526E-739.3752
GTTATTT22350.039.161072
TACGAAT700.038.57142612
CTAGGCG356.24548E-638.5714265
AGGCGAT3100.038.4677437
Produced by FastQC (version 0.11.5)