##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934582.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	758460
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77004060860164	31.0	31.0	34.0	30.0	34.0
2	31.9434643883659	33.0	31.0	34.0	30.0	34.0
3	30.832101890673208	31.0	31.0	34.0	26.0	34.0
4	35.15636289323102	37.0	35.0	37.0	32.0	37.0
5	31.676109485009096	37.0	35.0	37.0	0.0	37.0
6	33.768329246103946	37.0	35.0	37.0	17.0	37.0
7	21.208525169422252	32.0	0.0	37.0	0.0	37.0
8	28.39651398887219	35.0	17.0	37.0	17.0	37.0
9	35.11677082509295	37.0	32.0	39.0	32.0	39.0
10	36.57359781662843	37.0	35.0	39.0	33.0	39.0
11	36.99365688368536	39.0	37.0	39.0	34.0	39.0
12	37.38399256387944	39.0	37.0	39.0	35.0	39.0
13	37.27927642855259	39.0	37.0	39.0	34.0	39.0
14	38.820533713050125	40.0	38.0	41.0	35.0	41.0
15	38.93658070300346	40.0	38.0	41.0	35.0	41.0
16	38.95037839833346	40.0	38.0	41.0	35.0	41.0
17	38.90779606043826	40.0	38.0	41.0	35.0	41.0
18	38.652772723676925	40.0	38.0	41.0	35.0	41.0
19	38.420745985285976	40.0	37.0	41.0	35.0	41.0
20	38.16443583049864	40.0	36.0	41.0	34.0	41.0
21	38.051558421010995	40.0	36.0	41.0	34.0	41.0
22	37.881326635550984	39.0	35.0	41.0	34.0	41.0
23	37.719476307254176	39.0	35.0	41.0	34.0	41.0
24	37.482867916567784	39.0	35.0	41.0	33.0	41.0
25	37.32706405083986	39.0	35.0	41.0	33.0	41.0
26	37.21127152387733	39.0	35.0	41.0	33.0	41.0
27	37.15599108720302	39.0	35.0	41.0	33.0	41.0
28	37.05489149065211	39.0	35.0	41.0	33.0	41.0
29	37.01270996492894	39.0	35.0	41.0	33.0	41.0
30	36.81430925823379	39.0	35.0	41.0	33.0	41.0
31	36.56305935711837	39.0	35.0	41.0	32.0	41.0
32	36.20073042744509	39.0	35.0	41.0	30.0	41.0
33	35.62977744376764	38.0	35.0	40.0	25.0	41.0
34	34.97767977216992	38.0	34.0	40.0	21.0	41.0
35	34.37469082087387	38.0	33.0	40.0	18.0	41.0
36	33.9462410674261	38.0	33.0	40.0	15.0	41.0
37	33.685759301743005	38.0	33.0	40.0	12.0	41.0
38	33.47878332410411	38.0	33.0	40.0	10.0	41.0
39	33.31850460142921	38.0	33.0	40.0	10.0	41.0
40	33.19424491733249	38.0	33.0	40.0	10.0	41.0
41	33.041580307465125	38.0	33.0	40.0	10.0	41.0
42	32.88197927379163	38.0	32.0	40.0	10.0	41.0
43	32.77390633652401	38.0	32.0	40.0	10.0	41.0
44	32.61197821902276	38.0	31.0	40.0	9.0	41.0
45	32.53951559739472	38.0	31.0	40.0	8.0	41.0
46	32.38761569496084	38.0	31.0	40.0	8.0	41.0
47	32.33675078448435	38.0	31.0	40.0	8.0	41.0
48	32.21567122854204	37.0	31.0	40.0	8.0	41.0
49	32.10116024576115	37.0	30.0	40.0	8.0	41.0
50	31.974464045566016	37.0	30.0	40.0	8.0	41.0
51	30.502009334704532	35.0	26.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	14.0
9	21.0
10	28.0
11	28.0
12	17.0
13	27.0
14	19.0
15	23.0
16	44.0
17	111.0
18	238.0
19	513.0
20	1031.0
21	2097.0
22	4198.0
23	7941.0
24	14068.0
25	21578.0
26	26486.0
27	25848.0
28	21944.0
29	18509.0
30	17527.0
31	18515.0
32	22894.0
33	31546.0
34	42321.0
35	57497.0
36	72100.0
37	126264.0
38	134622.0
39	90370.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.712100835904334	17.879255333175117	21.712944651003347	29.695699179917202
2	44.90546633968832	20.309706510560872	21.52967855918572	13.255148590565092
3	25.67597500197769	20.08095351106189	40.81784141549983	13.425230071460593
4	21.793766315956017	20.96379505840783	42.48463992827572	14.75779869736044
5	18.526619729451784	32.71800754159745	35.56205996361047	13.193312765340295
6	21.035651187933443	28.365503783983335	39.24676317801862	11.352081850064605
7	40.24852991588218	40.98634074308467	16.906099201012577	1.8590301400205682
8	71.2753474144978	3.2376130580386575	23.724652585502202	1.7623869419613427
9	66.59164623051974	5.5430741238826045	24.65865042322601	3.2066292223716477
10	30.372069720222555	29.056904780739924	30.64499116631068	9.926034332726841
11	21.75711309759249	23.138332937795006	41.1930754423437	13.911478522268808
12	20.205416238166812	20.981726129261926	43.654114917068796	15.158742715502466
13	18.555757719589693	20.42032539619756	45.08741397041373	15.936502913799014
14	16.63581467710888	22.70007647074335	44.5552830735965	16.108825778551275
15	16.38741660733592	23.0696411148907	45.187880705640374	15.355061572133005
16	19.14985628774095	23.20082799356591	43.026263744956886	14.623051973736256
17	19.491996941170267	22.283838303931653	42.566911900429815	15.657252854468265
18	18.657806608126997	22.728555230335154	42.8556548796245	15.75798328191335
19	18.560899717849328	23.075574189805657	42.09477098330828	16.268755109036732
20	19.45837618331883	23.31276534029481	42.48108008332674	14.74777839305962
21	19.854178203201222	22.49004561875379	42.68544155261978	14.970334625425203
22	19.4513883395301	21.00427181393877	43.0195395933866	16.52480025314453
23	18.271761200326978	22.367296891068744	43.19977322469214	16.161168683912138
24	17.66606017456425	22.662500329615273	43.31672072357144	16.35471877224903
25	17.496506078105636	24.27194578487989	42.07077499143001	16.16077314558447
26	17.912876091026554	24.42739234765182	41.598370382090025	16.0613611792316
27	17.390238114073252	23.875088996123726	42.507976689607894	16.226696200195132
28	16.833847533159297	23.513830656857316	43.47902328402289	16.1732985259605
29	17.264061387548455	22.821770429554626	43.25158874561612	16.662579437280804
30	17.721567386546422	22.815046277984337	43.04274450860955	16.42064182685969
31	18.31883026131899	22.818737969042534	42.06853360757324	16.793898162065236
32	18.41375945995834	23.71502781952905	41.30079371357751	16.570419006935104
33	18.59517970624687	23.58819186245814	40.983703820900246	16.83292461039475
34	18.126862326292752	24.557128919125596	40.255913297998575	17.060095456583078
35	18.18672045987923	25.37404741186088	39.21156026685652	17.227671861403373
36	18.999024338791763	26.31951586108694	37.67832186272183	17.00313793739947
37	19.18598212166759	26.702792500593308	36.63700129209187	17.474224085647233
38	19.487909711784404	27.206180945600295	35.559027503098385	17.746881839516917
39	19.577037681618016	26.844527068006226	35.35638003322522	18.222055217150544
40	19.64876196503441	27.092793291669963	35.66384515992933	17.594599583366293
41	19.176489201803655	27.280278458982675	35.03282968119611	18.51040265801756
42	19.12796983361021	27.403422724995387	34.86643989135881	18.6021675500356
43	19.875141734567414	26.975318408353772	34.343538222187064	18.806001634891754
44	19.61052659336023	27.315085831817104	34.3412968383303	18.733090736492368
45	19.38019144055059	27.324183213353376	33.74152888748253	19.554096458613508
46	19.65350842496638	27.328665981066898	33.86243176963848	19.155393824328243
47	19.079318619307546	26.822640613875485	35.19658254884898	18.90145821796799
48	19.35698652532764	26.42472905624555	35.11312396171189	19.105160456714923
49	18.788993486802205	26.66297497560847	35.24483822482399	19.30319331276534
50	18.592410937953222	26.655327901273633	35.410832476333624	19.34142868443952
51	18.994541571078237	26.120691928381195	35.02610552962582	19.858660970914748
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	537.0
1	1003.0
2	1469.0
3	27886.5
4	54304.0
5	37169.5
6	20035.0
7	17461.0
8	14887.0
9	14148.0
10	13409.0
11	12982.0
12	12555.0
13	11981.5
14	11408.0
15	10756.5
16	10105.0
17	9581.0
18	9057.0
19	8577.0
20	8097.0
21	7894.5
22	7692.0
23	7741.5
24	7791.0
25	8465.0
26	10165.5
27	11192.0
28	12460.0
29	13728.0
30	15793.5
31	17859.0
32	19988.0
33	22117.0
34	24332.5
35	26548.0
36	28049.0
37	29550.0
38	31447.5
39	33345.0
40	34584.5
41	35824.0
42	37824.5
43	39825.0
44	41982.0
45	44139.0
46	46062.0
47	47985.0
48	45402.5
49	42820.0
50	41933.5
51	41047.0
52	38020.5
53	34994.0
54	32578.0
55	30162.0
56	28193.0
57	26224.0
58	24154.5
59	22085.0
60	20184.5
61	18284.0
62	16126.5
63	13969.0
64	11969.5
65	9970.0
66	8081.5
67	6193.0
68	4955.0
69	3717.0
70	3073.5
71	2430.0
72	2074.5
73	1719.0
74	1364.0
75	801.5
76	594.0
77	449.5
78	305.0
79	241.0
80	177.0
81	126.5
82	76.0
83	56.5
84	37.0
85	31.0
86	25.0
87	18.0
88	11.0
89	9.0
90	7.0
91	6.5
92	6.0
93	3.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	758460.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.29735527674627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5658014165377	32.58770441566935
2	8.721919940040689	7.727159725586399
3	4.264111203141983	5.666665467154034
4	2.9384409696097005	5.206606543621908
5	2.094318217170023	4.638637906427119
6	1.6363267848162313	4.349096936151633
7	1.3255508966830951	4.110287930544646
8	1.0950867595499212	3.880755779731494
9	0.9194893230862404	3.6657850696133525
>10	3.3895524339969	20.84385766747238
>50	0.028276180709951283	0.8796713988937148
>100	0.01917579391987783	1.5921903891878308
>500	9.750403688068149E-4	0.30507744232642936
>1k	3.250134562689383E-4	0.27959433364696834
>5k	0.0	0.0
>10k+	6.500269125378766E-4	4.2669089939728115
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18738	2.4705323945890356	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10899	1.4369907444031327	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1942	0.2560451441077974	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	856	0.11286026949344724	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009888458191598766	0.0
2	0.0	0.0	0.0	0.06078105635102708	0.0
3	0.0	0.0	0.0	0.10824565567070116	0.0
4	0.0	0.0	0.0	0.1766737863565646	0.0
5	0.0	0.0	0.0	0.4124146296442792	0.0
6	0.0	0.0	0.0	0.6235002505076075	0.0
7	0.0	0.0	0.0	0.7992511140996229	0.0
8	0.0	0.0	0.0	1.1082983941143896	0.0
9	0.0	0.0	0.0	1.2914326398227989	0.0
10	0.0	0.0	0.0	1.642670674788387	0.0
11	0.0	0.0	0.0	1.8413627613849115	0.0
12	0.0	0.0	0.0	2.091211138359307	0.0
13	0.0	0.0	0.0	2.1887772591830816	0.0
14	0.0	0.0	0.0	2.251931545500092	0.0
15	0.0	0.0	0.0	2.3198322917490706	0.0
16	0.0	0.0	0.0	2.4034227249953855	0.0
17	0.0	0.0	0.0	2.503889460222029	0.0
18	0.0	0.0	0.0	2.619914036336788	0.0
19	0.0	0.0	0.0	2.7151069271945785	0.0
20	0.0	0.0	0.0	2.804498589246631	0.0
21	0.0	0.0	0.0	2.91050286106057	0.0
22	0.0	0.0	0.0	3.0146612873454104	0.0
23	0.0	0.0	0.0	3.1335864778630382	0.0
24	0.0	0.0	0.0	3.2358990586187804	0.0
25	0.0	0.0	0.0	3.3296416422751363	0.0
26	0.0	0.0	0.0	3.425625609788255	0.0
27	0.0	0.0	0.0	3.5233235767212507	0.0
28	0.0	0.0	0.0	3.6301189251905175	0.0
29	0.0	0.0	0.0	3.746670885742162	0.0
30	0.0	0.0	0.0	3.887350684281307	0.0
31	0.0	0.0	0.0	4.01682356353664	0.0
32	0.0	0.0	0.0	4.163304590881523	0.0
33	0.0	0.0	0.0	4.288031010204889	0.0
34	0.0	0.0	0.0	4.420008965535427	0.0
35	0.0	0.0	0.0	4.556074150251826	0.0
36	0.0	0.0	0.0	4.7054557919995785	0.0
37	0.0	0.0	0.0	4.85576035651188	0.0
38	0.0	0.0	0.0	5.004746459931967	0.0
39	0.0	0.0	0.0	5.167444558711073	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	20	7.031768E-4	45.0	2
AGTGCGA	25	3.8895458E-5	45.0	1
ACTAGCG	25	3.8895458E-5	45.0	1
TTAACGG	25	3.8895458E-5	45.0	2
GCGCTAA	25	3.8895458E-5	45.0	1
ACGAGAC	20	7.031768E-4	45.0	4
ATCGCTC	20	7.031768E-4	45.0	19
CCGCATT	20	7.031768E-4	45.0	30
CGTTTTA	4495	0.0	44.44939	1
CGTTATT	1955	0.0	44.079285	1
CGTTTTT	8155	0.0	43.81361	1
CGGTTTA	275	0.0	42.545456	1
GTTTTAT	4795	0.0	41.996872	2
CGAATAT	65	0.0	41.53846	14
CGGTTTT	815	0.0	40.306747	1
CGCTAAG	40	3.4561526E-7	39.375	2
GTTATTT	2235	0.0	39.16107	2
TACGAAT	70	0.0	38.571426	12
CTAGGCG	35	6.24548E-6	38.571426	5
AGGCGAT	310	0.0	38.467743	7
>>END_MODULE