##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934581.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	339731
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96634984737925	33.0	31.0	34.0	30.0	34.0
2	32.17104120613073	33.0	31.0	34.0	30.0	34.0
3	32.02497564249362	33.0	31.0	34.0	30.0	34.0
4	35.80229946634249	37.0	35.0	37.0	35.0	37.0
5	32.062832064191966	37.0	35.0	37.0	0.0	37.0
6	33.95361624343966	37.0	35.0	37.0	19.0	37.0
7	20.815521692162328	32.0	0.0	37.0	0.0	37.0
8	28.134412226143624	35.0	17.0	37.0	17.0	37.0
9	34.84807097379986	37.0	32.0	39.0	32.0	39.0
10	36.27814359007568	37.0	35.0	39.0	32.0	39.0
11	36.68270778939808	38.0	35.0	39.0	33.0	39.0
12	36.94522136631629	39.0	37.0	39.0	33.0	39.0
13	36.62068813266967	39.0	35.0	39.0	33.0	39.0
14	37.91089420747591	40.0	37.0	41.0	33.0	41.0
15	38.13920719628177	40.0	37.0	41.0	33.0	41.0
16	38.253500563681264	40.0	37.0	41.0	34.0	41.0
17	38.25993212276772	40.0	37.0	41.0	34.0	41.0
18	38.154725356237726	40.0	37.0	41.0	34.0	41.0
19	38.10214552101515	40.0	37.0	41.0	34.0	41.0
20	38.05536439123895	40.0	37.0	41.0	34.0	41.0
21	37.955606053024304	40.0	36.0	41.0	33.0	41.0
22	37.95065213359982	40.0	36.0	41.0	33.0	41.0
23	37.91351392719534	40.0	36.0	41.0	34.0	41.0
24	37.81256346933309	40.0	36.0	41.0	33.0	41.0
25	37.67056288651904	40.0	36.0	41.0	33.0	41.0
26	37.54291189205578	39.0	35.0	41.0	33.0	41.0
27	37.463157615878444	39.0	35.0	41.0	33.0	41.0
28	37.37781951014185	39.0	35.0	41.0	33.0	41.0
29	37.36914794352002	39.0	35.0	41.0	33.0	41.0
30	37.2025484868911	39.0	35.0	41.0	32.0	41.0
31	37.06203437425493	39.0	35.0	41.0	32.0	41.0
32	36.94758205756908	39.0	35.0	41.0	31.0	41.0
33	36.832835390352955	39.0	35.0	41.0	31.0	41.0
34	36.64402718621498	39.0	35.0	41.0	31.0	41.0
35	36.47580880166956	39.0	35.0	41.0	30.0	41.0
36	36.29324377227866	39.0	35.0	41.0	30.0	41.0
37	36.21095219453038	39.0	35.0	41.0	30.0	41.0
38	36.07757902575862	39.0	35.0	40.0	29.0	41.0
39	36.0062637792842	39.0	35.0	40.0	29.0	41.0
40	35.854061007090905	39.0	35.0	40.0	28.0	41.0
41	35.722601116765915	39.0	35.0	40.0	27.0	41.0
42	35.626460346568315	38.0	35.0	40.0	27.0	41.0
43	35.53904118258269	38.0	35.0	40.0	27.0	41.0
44	35.401688394641646	38.0	34.0	40.0	26.0	41.0
45	35.29558385899432	38.0	34.0	40.0	26.0	41.0
46	35.18473439279901	38.0	34.0	40.0	26.0	41.0
47	35.13754117228042	38.0	34.0	40.0	26.0	41.0
48	35.01943302200859	38.0	34.0	40.0	25.0	41.0
49	34.91495624479367	38.0	34.0	40.0	24.0	41.0
50	34.732123356420225	37.0	34.0	40.0	24.0	41.0
51	32.86267664711198	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	24.0
10	21.0
11	14.0
12	19.0
13	21.0
14	18.0
15	21.0
16	31.0
17	65.0
18	112.0
19	228.0
20	431.0
21	679.0
22	1053.0
23	1644.0
24	2491.0
25	3373.0
26	4120.0
27	4763.0
28	5164.0
29	6024.0
30	7218.0
31	9316.0
32	12072.0
33	18214.0
34	24961.0
35	31603.0
36	38747.0
37	63367.0
38	63537.0
39	40362.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.29255793554312	20.84060624435215	25.740953872328404	14.125881947776328
2	32.140134400452126	24.43433186844886	25.97702299760693	17.448510733492085
3	31.95793142221346	23.72112053359864	27.580055985470857	16.740892058717044
4	27.934748374449196	26.34437245938698	26.52186582914129	19.199013337022528
5	23.15861667024793	36.73788968330827	23.229555148043598	16.873938498400204
6	26.779422543129712	33.17330476170853	25.44925249682837	14.598020198333387
7	49.13534531732459	42.91542426213681	5.2406168409712395	2.7086135795673636
8	84.47830783767158	4.603642293461592	7.925682378116804	2.9923674907500346
9	77.95226223100042	7.7078629857151695	9.63762506218155	4.702249721102873
10	41.88931831360694	29.83419234629751	16.054760972651895	12.221728367443655
11	34.1084563963842	24.054914034927634	24.60534952653717	17.231280042150996
12	31.199684456231548	22.172542393835123	28.66591509164604	17.96185805828729
13	25.45013554841919	26.021764278208348	29.456834966488195	19.07126520688427
14	21.1705731887876	27.898837609755955	30.801133838242613	20.129455363213836
15	19.914579476114927	25.187869225946415	35.424203266702186	19.47334803123648
16	24.348087163079025	23.080319429195452	32.70440436698447	19.867189040741057
17	25.067479859064967	23.44796324150578	28.473409844847836	23.011147054581418
18	25.815718907017608	23.016445364126323	30.56418166137326	20.6036540674828
19	26.385581533625135	25.312085149721398	27.84938672067018	20.452946595983292
20	27.345752963373965	25.30973034547922	28.385399036296366	18.959117654850456
21	26.219567834551455	24.830233331665347	29.549555383524023	19.400643450259174
22	25.483397158339983	23.006143095566784	29.033558903956365	22.476900842136867
23	22.134865525960244	25.503707344928785	29.664352090330294	22.69707503878068
24	22.34679790775643	24.347204111488207	32.46215388057021	20.843844100185148
25	22.591697548943134	25.825726825046875	30.4022888697234	21.18028675628659
26	22.88693113080643	26.89245314675434	28.564952859762577	21.655662862676646
27	21.866123491821472	26.61811845254039	30.279839049130047	21.23591900650809
28	20.613073284451524	26.556893542243714	31.19556354880773	21.63446962449703
29	21.757508146150926	25.503707344928785	30.851467778919208	21.88731673000109
30	22.89487859512379	25.044814868233985	30.904156523837976	21.156150012804247
31	24.53146754343878	25.328568779416656	29.088308102587046	21.05165557455752
32	25.25056588889445	25.732123356420228	28.724785197700538	20.292525556984792
33	25.879004271026197	25.15578501814671	27.56622151054805	21.398989200279043
34	22.44864319123071	25.747723934524668	29.588409653519992	22.21522322072463
35	22.13515987649052	26.626948968448566	29.874812719475113	21.363078435585802
36	25.683555518925267	26.13008527334862	28.126959270717126	20.059399937008987
37	23.54892547338924	27.618615904936554	28.039537163226196	20.792921458448006
38	22.50957375099711	27.62509161660255	27.84938672067018	22.015947911730162
39	22.727687493929018	26.05620329025023	28.77335303519549	22.44275618062526
40	24.56001954487521	26.09594061183701	28.471938092196474	20.872101751091304
41	21.730722247896132	25.49252202477843	28.851061575187426	23.925694152138018
42	22.97582499094872	25.616737948553414	28.132551930792303	23.27488512970556
43	22.86250003679382	25.587891596586708	29.312897557184947	22.236710809434523
44	21.758685548272016	25.572291018482268	29.450359254822196	23.218664178423516
45	21.80754773629725	27.25185514421704	27.634216483040998	23.306380636444718
46	22.157236166260954	26.855953681000557	28.22144579093459	22.765364361803897
47	21.469044626483893	27.18179971801219	29.513055917770238	21.836099737733676
48	21.829918376597956	25.966720729047392	30.33135039192773	21.87201050242692
49	23.12535506032714	25.832202536712867	28.970273539947783	22.072168863012205
50	20.903008556769915	27.457017463816964	29.28876081370261	22.35121316571052
51	21.123477103944	27.633333431450176	27.602426625771564	23.640762838834252
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	290.0
1	722.0
2	1154.0
3	2414.5
4	3675.0
5	2562.5
6	1450.0
7	1312.5
8	1175.0
9	1197.0
10	1219.0
11	1250.5
12	1282.0
13	1226.5
14	1171.0
15	1157.0
16	1143.0
17	1130.5
18	1118.0
19	1205.0
20	1292.0
21	1414.0
22	1536.0
23	1665.0
24	1794.0
25	2104.0
26	2890.5
27	3367.0
28	4157.5
29	4948.0
30	5811.5
31	6675.0
32	7843.0
33	9011.0
34	9909.5
35	10808.0
36	11478.0
37	12148.0
38	13441.5
39	14735.0
40	16320.0
41	17905.0
42	19439.5
43	20974.0
44	22119.5
45	23265.0
46	23514.0
47	23763.0
48	24060.5
49	24358.0
50	23886.5
51	23415.0
52	22072.5
53	20730.0
54	19789.5
55	18849.0
56	18328.0
57	17807.0
58	16617.0
59	15427.0
60	14493.5
61	13560.0
62	12290.0
63	11020.0
64	9668.5
65	8317.0
66	7175.0
67	6033.0
68	5230.0
69	4427.0
70	3726.0
71	3025.0
72	2438.5
73	1852.0
74	1553.5
75	967.5
76	680.0
77	500.5
78	321.0
79	254.0
80	187.0
81	131.5
82	76.0
83	54.5
84	33.0
85	29.0
86	25.0
87	17.0
88	9.0
89	7.5
90	6.0
91	4.0
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	339731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.97321110982496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.23713536530117	20.206035658697008
2	8.665448004063022	5.367935007821106
3	4.3803187688933605	4.070176138717879
4	3.1446357218612553	3.8959786430682186
5	2.466990392343754	3.8205307113986495
6	1.9631372929124826	3.648279948656917
7	1.7337776397374045	3.759046259715648
8	1.5822269071886386	3.92053184159993
9	1.3289897696053241	3.7046772627105007
>10	9.449484550821932	45.82503107627871
>50	0.039879656059725016	0.8059686564966102
>100	0.005981948408958752	0.4029843282483051
>500	9.969914014931254E-4	0.2220273808509819
>1k	9.969914014931254E-4	0.3507970857395208
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1136	0.3343822023895376	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	719	0.21163803126591332	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.943505302724803E-4	0.0	0.0	0.025608496133705784	0.0
2	2.943505302724803E-4	0.0	0.0	0.13069163544098125	0.0
3	2.943505302724803E-4	0.0	0.0	0.16630804960395137	0.0
4	2.943505302724803E-4	0.0	0.0	0.21870244399245287	0.0
5	2.943505302724803E-4	0.0	0.0	0.3720590702644151	0.0
6	2.943505302724803E-4	0.0	0.0	0.4836179212376851	0.0
7	2.943505302724803E-4	0.0	0.0	0.6022411849374947	0.0
8	2.943505302724803E-4	0.0	0.0	0.8753984770303563	0.0
9	2.943505302724803E-4	0.0	0.0	0.971356749899185	0.0
10	2.943505302724803E-4	0.0	0.0	1.2489292999461339	0.0
11	2.943505302724803E-4	0.0	0.0	1.643064659980985	0.0
12	2.943505302724803E-4	0.0	0.0	1.948306159873547	0.0
13	2.943505302724803E-4	0.0	0.0	2.075759939481531	0.0
14	2.943505302724803E-4	0.0	0.0	2.116380312659133	0.0
15	2.943505302724803E-4	0.0	0.0	2.216753843482049	0.0
16	2.943505302724803E-4	0.0	0.0	2.448407710806491	0.0
17	2.943505302724803E-4	0.0	0.0	2.713911889112268	0.0
18	2.943505302724803E-4	0.0	0.0	3.010028522566383	0.0
19	2.943505302724803E-4	0.0	0.0	3.1889936449720513	0.0
20	2.943505302724803E-4	0.0	0.0	3.3679587673777194	0.0
21	2.943505302724803E-4	0.0	0.0	3.600495686292979	0.0
22	2.943505302724803E-4	0.0	0.0	3.846867080131045	0.0
23	2.943505302724803E-4	0.0	0.0	4.108839052073552	0.0
24	2.943505302724803E-4	0.0	0.0	4.320771433869738	0.0
25	2.943505302724803E-4	0.0	0.0	4.506506618471673	0.0
26	2.943505302724803E-4	0.0	0.0	4.679879080802164	0.0
27	2.943505302724803E-4	0.0	0.0	4.882686596159903	0.0
28	2.943505302724803E-4	0.0	0.0	5.07460314189756	0.0
29	2.943505302724803E-4	0.0	0.0	5.295954740662466	0.0
30	2.943505302724803E-4	0.0	0.0	5.543209186091349	0.0
31	2.943505302724803E-4	0.0	0.0	5.768092991219524	0.0
32	2.943505302724803E-4	0.0	0.0	5.990916342635791	0.0
33	2.943505302724803E-4	0.0	0.0	6.22345326155105	0.0
34	2.943505302724803E-4	0.0	0.0	6.468352902737754	0.0
35	2.943505302724803E-4	0.0	0.0	6.734151431573804	0.0
36	2.943505302724803E-4	0.0	0.0	6.957269133520344	0.0
37	2.943505302724803E-4	0.0	0.0	7.196870465162143	0.0
38	2.943505302724803E-4	0.0	0.0	7.47061645831555	0.0
39	2.943505302724803E-4	0.0	0.0	7.857687405623861	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTAG	25	3.8849845E-5	45.000004	2
ACGATAG	25	3.8849845E-5	45.000004	2
CGGTCTA	25	3.8849845E-5	45.000004	31
TCGTGAC	20	7.026263E-4	45.0	17
GTATGCG	20	7.026263E-4	45.0	1
CTACGAT	20	7.026263E-4	45.0	27
CGCTAAG	20	7.026263E-4	45.0	2
TATTACG	20	7.026263E-4	45.0	1
GCGATAC	20	7.026263E-4	45.0	9
GCTACGA	70	0.0	45.0	10
AATAGGC	20	7.026263E-4	45.0	4
TCGACGT	35	1.2085547E-7	45.0	26
CGTCATA	20	7.026263E-4	45.0	38
TAGTCTC	20	7.026263E-4	45.0	42
ATCTGCG	20	7.026263E-4	45.0	14
CGCTTAG	20	7.026263E-4	45.0	2
AGGCGAT	115	0.0	44.999996	7
CGAATAT	60	0.0	44.999996	14
GCGGCTA	30	2.1609721E-6	44.999996	24
TAATCGT	30	2.1609721E-6	44.999996	21
>>END_MODULE