FastQCFastQC Report
Sat 14 Jan 2017
SRR2934580.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934580.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557193
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122102.191341240826787No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69311.2439136887936495No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13290.23851699500891077No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG207.030152E-445.0000042
CGTATAG406.8048394E-945.0000042
CGGTTTA2100.045.01
CGGTTAG253.88821E-545.031
GCGTTAA253.88821E-545.01
ACGGGCG502.1827873E-1145.05
CGTTAAG253.88821E-545.02
CTTTGCG302.1634805E-644.9999961
ATAACGG302.1634805E-644.9999962
CGTTTTA26750.044.5794371
CGTTATT11750.043.8510631
CGTTTTT54350.043.509661
GTTTTAT29350.040.7836462
CGTAAAG451.9252184E-840.02
CTTACGG451.9252184E-840.02
ATAGGAT2500.039.6000025
GCGAGAC403.4539516E-739.37500421
CGAATAT403.4539516E-739.37500414
CGGTTTT5850.039.230771
CGGGCAT356.242495E-638.5714266
Produced by FastQC (version 0.11.5)