FastQCFastQC Report
Sat 14 Jan 2017
SRR2934579.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934579.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720554
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121901.691753844958185No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69130.9594006833630789No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26030.3612498161137125No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13540.1879109685047894No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG453.8380676E-1045.0000042
GCGAACG207.0315314E-445.00000411
CGCATAG207.0315314E-445.0000042
CGTTTTA28000.044.5178571
CGTTTTT54650.043.929551
CGTTATT13350.043.6516841
CGGTTTA2100.041.7857131
GTTTTAT30850.041.4262582
GGCACCG1650.040.909098
GCGATAT451.9266736E-840.0000049
AGGCGAT3800.039.6710557
GCGATGC1900.037.8947379
GTTATTT15450.037.864082
TGCGATA301.1394991E-437.5000041
CGGTTTT5600.036.9642871
TCGTAAC1350.036.66666434
CGTAACC1350.036.66666435
TTAACGG800.036.5625042
ATAGGCG1050.036.428575
CTGTCGA250.002106587636.025
Produced by FastQC (version 0.11.5)