##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934579.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	720554
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.702773143997536	31.0	31.0	34.0	30.0	34.0
2	31.889247717728303	33.0	31.0	34.0	30.0	34.0
3	31.11713070776097	31.0	31.0	34.0	27.0	34.0
4	35.26539162921863	37.0	35.0	37.0	33.0	37.0
5	31.741096434132626	37.0	35.0	37.0	0.0	37.0
6	33.75437510582135	37.0	35.0	37.0	17.0	37.0
7	21.206234369665562	32.0	0.0	37.0	0.0	37.0
8	28.35336005351438	35.0	17.0	37.0	17.0	37.0
9	34.974526544852985	37.0	32.0	39.0	32.0	39.0
10	36.307806215772864	37.0	35.0	39.0	32.0	39.0
11	36.73384506921064	39.0	37.0	39.0	33.0	39.0
12	37.08979063331825	39.0	37.0	39.0	34.0	39.0
13	36.899602250490595	39.0	37.0	39.0	33.0	39.0
14	38.24223444738354	40.0	38.0	41.0	34.0	41.0
15	38.41818101072231	40.0	38.0	41.0	34.0	41.0
16	38.498383188491076	40.0	38.0	41.0	34.0	41.0
17	38.483978993940774	40.0	38.0	41.0	34.0	41.0
18	38.29556147075722	40.0	38.0	41.0	34.0	41.0
19	38.11275629585014	40.0	37.0	41.0	34.0	41.0
20	37.90588769197034	40.0	36.0	41.0	34.0	41.0
21	37.79080818370309	40.0	36.0	41.0	33.0	41.0
22	37.70948325871482	39.0	35.0	41.0	33.0	41.0
23	37.59412202277692	39.0	35.0	41.0	33.0	41.0
24	37.397971560771296	39.0	35.0	41.0	33.0	41.0
25	37.2382944234575	39.0	35.0	41.0	33.0	41.0
26	37.104269770204596	39.0	35.0	41.0	33.0	41.0
27	37.042117592852165	39.0	35.0	41.0	33.0	41.0
28	36.94443303347147	39.0	35.0	41.0	33.0	41.0
29	36.88482334426011	39.0	35.0	40.0	32.0	41.0
30	36.66613744424429	39.0	35.0	40.0	31.0	41.0
31	36.406095865126	38.0	35.0	40.0	31.0	41.0
32	36.11748598994662	38.0	35.0	40.0	30.0	41.0
33	35.69734260027701	38.0	35.0	40.0	27.0	41.0
34	35.183250665460186	38.0	34.0	40.0	24.0	41.0
35	34.72946926947876	38.0	34.0	40.0	21.0	41.0
36	34.38257507417904	38.0	34.0	40.0	18.0	41.0
37	34.16451369363018	38.0	33.0	40.0	17.0	41.0
38	33.95910368966101	38.0	33.0	40.0	15.0	41.0
39	33.820200845460576	38.0	33.0	40.0	15.0	41.0
40	33.718340332577434	38.0	33.0	40.0	15.0	41.0
41	33.56231455241384	38.0	33.0	40.0	13.0	41.0
42	33.418097741460045	38.0	33.0	40.0	12.0	41.0
43	33.33783866302873	38.0	33.0	40.0	12.0	41.0
44	33.18106345950477	38.0	33.0	40.0	10.0	41.0
45	33.10750616886451	38.0	32.0	40.0	10.0	41.0
46	33.00346816477322	37.0	32.0	40.0	10.0	41.0
47	32.93682777418486	37.0	32.0	40.0	10.0	41.0
48	32.789907487849625	37.0	32.0	40.0	10.0	41.0
49	32.679250965229535	37.0	31.0	40.0	10.0	41.0
50	32.504441027320645	37.0	31.0	40.0	10.0	41.0
51	30.834711902230783	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	15.0
9	34.0
10	34.0
11	29.0
12	30.0
13	12.0
14	20.0
15	27.0
16	67.0
17	169.0
18	358.0
19	654.0
20	1274.0
21	2422.0
22	4324.0
23	7199.0
24	12010.0
25	17327.0
26	20487.0
27	19960.0
28	17817.0
29	16957.0
30	17523.0
31	20234.0
32	25047.0
33	34817.0
34	46446.0
35	59562.0
36	75318.0
37	121189.0
38	121122.0
39	78062.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.44023071136931	18.260671649869405	22.661174596213467	23.63792304254782
2	40.6176358746187	21.41518886856502	23.161900426616185	14.805274830200096
3	28.37261329477041	20.76929695761872	36.6376427026982	14.220447044912666
4	24.28645181346575	23.050319615184982	36.917566206002604	15.745662365346663
5	20.155880058954637	34.238377692719766	31.483414150778426	14.122328097547165
6	22.873649997085575	31.041115586062944	33.713226212053506	12.372008204797975
7	43.71913832967411	41.21578674186806	12.847059346003215	2.218015582454611
8	76.30489873069888	3.5948728339583154	17.832528859738478	2.267699575604327
9	71.38895905095247	5.893382036599617	19.204528737610225	3.513130174837694
10	35.30824893068389	26.75496909322549	26.16278585643824	11.773996119652379
11	26.967860840408907	23.024089797572422	34.579920450098115	15.428128911920549
12	24.70973722996472	20.920985797039503	37.748732225482065	16.62054474751372
13	21.802252155979982	21.651257227078055	39.26978408280296	17.27670653413901
14	18.307996347255028	23.684137483103278	40.222939571496376	17.784926598145315
15	17.847794891153196	23.80293496393053	41.25020470360306	17.099065441313215
16	22.107156437963013	23.068638852882643	38.05585702112541	16.768347688028935
17	22.676024281316877	22.43704149862467	36.65915392878257	18.22778029127588
18	22.380279618182676	22.811614396700318	37.705848555417084	17.102257429699925
19	21.752429380726497	24.595797122769426	35.850193045906344	17.801580450597733
20	21.653338958634606	25.275551867035638	36.58268498960522	16.488424184724533
21	21.842221401865785	24.030676396217356	37.5644295916753	16.562672610241563
22	22.027773074606483	21.909669504298083	37.594128961882106	18.468428459213328
23	19.99572551120388	23.29277195047144	38.30524846160038	18.4062540767243
24	19.53927117190384	23.76712918115783	38.40572670472997	18.287872942208356
25	20.170452179850503	24.7991129047927	36.45986282776864	18.57057208758816
26	19.932440871884687	25.1702162502741	35.747910635427736	19.149432242413475
27	18.939593701513	24.606760908967267	38.05974292003098	18.393902469488754
28	18.514087771353708	24.29824829228621	37.778292813585104	19.409371122774978
29	19.685269945070043	23.88093050624936	37.18999547570342	19.24380407297718
30	20.388756429080956	23.680112802093944	37.151969179270395	18.779161589554704
31	20.98996050261327	23.78364425150648	36.478043283362524	18.748351962517727
32	22.037487821870393	23.978355543096004	35.89196645914116	18.09219017589244
33	21.55508122916534	24.102010397555215	35.62855802618541	18.71435034709404
34	19.892194061791344	25.22836595175379	35.918335058857494	18.96110492759738
35	20.511856155125084	26.212192285380414	34.88940454150556	18.386547017988935
36	22.492693122236503	25.584064483716695	33.01223780591045	18.91100458813635
37	20.663822558753402	27.848016942519227	33.05373365493773	18.434426843789637
38	21.23407822314497	26.914568512561164	32.680548577899785	19.17080468639408
39	20.595402981594717	27.0962342863963	32.584511362090836	19.723851369918147
40	21.97170510468334	26.076463387893206	32.797958237689336	19.15387326973412
41	20.651470951517858	25.93712615570797	32.58798091468509	20.823421978089083
42	20.638841780074777	26.086872045675968	32.75604604235075	20.51824013189851
43	20.2816166449704	26.302123088623475	33.086625013531254	20.329635252874873
44	20.504361921521497	26.93385922498522	32.152482673054344	20.40929618043894
45	20.13006658765339	26.367350677395446	31.715041481970818	21.787541252980347
46	20.51449301509672	26.94315762593782	31.95055471206877	20.591794646896695
47	20.132148319209943	26.820613028308777	32.747857898228304	20.299380754252976
48	20.12007427618194	26.794799557007526	32.670139920117016	20.414986246693516
49	20.945827793614356	25.815691814909087	32.57812738531741	20.66035300615915
50	19.577019904129322	26.818531296752223	32.519422555422636	21.085026243695822
51	19.894969703866746	26.37165292261232	31.85548897098621	21.87788840253472
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	622.0
1	1062.5
2	1503.0
3	17518.0
4	33533.0
5	23197.0
6	12861.0
7	11237.5
8	9614.0
9	9212.5
10	8811.0
11	8664.5
12	8518.0
13	8077.5
14	7637.0
15	7283.0
16	6929.0
17	6646.0
18	6363.0
19	6018.0
20	5673.0
21	5592.5
22	5512.0
23	5766.5
24	6021.0
25	6550.5
26	7984.0
27	8888.0
28	10087.5
29	11287.0
30	13052.5
31	14818.0
32	16974.5
33	19131.0
34	21228.5
35	23326.0
36	25129.5
37	26933.0
38	28903.5
39	30874.0
40	32184.5
41	33495.0
42	35851.5
43	38208.0
44	41098.5
45	43989.0
46	46351.0
47	48713.0
48	47958.5
49	47204.0
50	45608.0
51	44012.0
52	40955.0
53	37898.0
54	36193.0
55	34488.0
56	32701.0
57	30914.0
58	29028.5
59	27143.0
60	24665.5
61	22188.0
62	19804.0
63	17420.0
64	15312.0
65	13204.0
66	11242.0
67	9280.0
68	7715.5
69	6151.0
70	5191.0
71	4231.0
72	3404.0
73	2577.0
74	2105.0
75	1258.5
76	884.0
77	670.0
78	456.0
79	377.5
80	299.0
81	208.5
82	118.0
83	89.5
84	61.0
85	40.0
86	19.0
87	16.0
88	13.0
89	11.5
90	10.0
91	8.5
92	7.0
93	4.5
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	720554.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.51708998289586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2420855705614	33.61350788240662
2	7.406710490687746	6.446369738010348
3	3.2788668814007633	4.280602353595624
4	1.9309010014170371	3.361087705069157
5	1.43051671937878	3.112596239962168
6	1.1980662361702525	3.128181372293414
7	1.0054593544584023	3.0628265641477475
8	0.8940011038463255	3.1123461184711014
9	0.770773069106751	3.0187620904240164
>10	4.803780647847763	31.60870233133878
>50	0.023793216311301664	0.6865691937572329
>100	0.013296207788710868	0.970393684640484
>500	3.4990020496607167E-4	0.0867918406158908
>1k	6.998004099321433E-4	0.6025180935387366
>5k	3.4990020496607167E-4	1.0526175336450054
>10k+	3.4990020496607167E-4	1.8561272580836996
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12190	1.691753844958185	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6913	0.9594006833630789	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2603	0.3612498161137125	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1354	0.1879109685047894	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01984584083913211	0.0
2	0.0	0.0	0.0	0.07563624655473428	0.0
3	0.0	0.0	0.0	0.1285122280911632	0.0
4	0.0	0.0	0.0	0.18527410853315643	0.0
5	0.0	0.0	0.0	0.3538943646138943	0.0
6	0.0	0.0	0.0	0.588713684192996	0.0
7	0.0	0.0	0.0	0.8077118439423	0.0
8	0.0	0.0	0.0	1.2541738717708875	0.0
9	0.0	0.0	0.0	1.5111983279532137	0.0
10	0.0	0.0	0.0	1.8742523114159384	0.0
11	0.0	0.0	0.0	2.2324489212467076	0.0
12	0.0	0.0	0.0	2.511262167720948	0.0
13	0.0	0.0	0.0	2.658509979821082	0.0
14	0.0	0.0	0.0	2.768980534422125	0.0
15	0.0	0.0	0.0	2.8623808902594394	0.0
16	0.0	0.0	0.0	3.022535438010198	0.0
17	0.0	0.0	0.0	3.2051726865717214	0.0
18	0.0	0.0	0.0	3.412096803293022	0.0
19	0.0	0.0	0.0	3.5658673742703533	0.0
20	0.0	0.0	0.0	3.727409743058813	0.0
21	0.0	0.0	0.0	3.919345392572937	0.0
22	0.0	0.0	0.0	4.1280736766432495	0.0
23	0.0	0.0	0.0	4.340965423826667	0.0
24	0.0	0.0	0.0	4.519716773482626	0.0
25	0.0	0.0	0.0	4.690973889534996	0.0
26	0.0	0.0	0.0	4.866116904492932	0.0
27	0.0	0.0	0.0	5.064714094988023	0.0
28	1.3878210377015463E-4	0.0	0.0	5.251237242455111	0.0
29	1.3878210377015463E-4	0.0	0.0	5.459965526525424	0.0
30	1.3878210377015463E-4	0.0	0.0	5.743497364527849	0.0
31	1.3878210377015463E-4	0.0	0.0	5.972349053644835	0.0
32	1.3878210377015463E-4	0.0	0.0	6.223822225676354	0.0
33	1.3878210377015463E-4	0.0	0.0	6.44420820646336	0.0
34	1.3878210377015463E-4	0.0	0.0	6.673892588202966	0.0
35	1.3878210377015463E-4	0.0	0.0	6.924810631819406	0.0
36	1.3878210377015463E-4	0.0	0.0	7.168373223936027	0.0
37	1.3878210377015463E-4	0.0	0.0	7.41373998340166	0.0
38	1.3878210377015463E-4	0.0	0.0	7.706153876045376	0.0
39	2.7756420754030927E-4	0.0	0.0	8.206879706448094	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	45	3.8380676E-10	45.000004	2
GCGAACG	20	7.0315314E-4	45.000004	11
CGCATAG	20	7.0315314E-4	45.000004	2
CGTTTTA	2800	0.0	44.517857	1
CGTTTTT	5465	0.0	43.92955	1
CGTTATT	1335	0.0	43.651684	1
CGGTTTA	210	0.0	41.785713	1
GTTTTAT	3085	0.0	41.426258	2
GGCACCG	165	0.0	40.90909	8
GCGATAT	45	1.9266736E-8	40.000004	9
AGGCGAT	380	0.0	39.671055	7
GCGATGC	190	0.0	37.894737	9
GTTATTT	1545	0.0	37.86408	2
TGCGATA	30	1.1394991E-4	37.500004	1
CGGTTTT	560	0.0	36.964287	1
TCGTAAC	135	0.0	36.666664	34
CGTAACC	135	0.0	36.666664	35
TTAACGG	80	0.0	36.562504	2
ATAGGCG	105	0.0	36.42857	5
CTGTCGA	25	0.0021065876	36.0	25
>>END_MODULE